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Breaking the 2-dimensional sequencing barrier with ultra low-cost (<$10), fast 3D image-based sequencing

Objective

A huge variety and depth of applications in healthcare, basic research, and data storage could be brought to the market by highly efficient, ultra-high-throughput and ubiquitously available low-cost and accurate DNA sequencing. State-of-the-art next generation sequencing (NGS) is still too low-capacity and too expensive, at ~€200/genome, to serve this vast potential market in healthcare, research, and drug discovery, already estimated at over €20B. Single Technologies’ 3D sequencing is an imaging-based, massively parallel sequencing system that uniquely employs a hydrogel layer on a cylindrical surface to embed and sequence in 3 dimensions, not only DNA fragments, but also barcode-labelled RNAs, proteins, single cells, or whole tissues. Rather than being restricted to a small 2D flow-cell surface or tissue sections manually placed onto a slide, as in competitors’ systems, sample embedded in the spinning cylinder (a toroid) is imaged via a parallel array of 128 or 256 detectors that sweep across the large area and depth of the hydrogel. Sequencing-by-synthesis proceeds at unprecedented throughput, due to extremely fast, high-resolution confocal imaging. The open fluidics and reusability of Single’s platform greatly lowers costs by eliminating the most expensive and restrictive part of state-of-art NGS: the flow-cell. We have demonstrated sequencing quality of Q30—a standard in the field—and have collaborations ongoing using Single’s platform with SciLifeLab at Karolinska Institute, the Genome Institute of Singapore, and other consortia. Pharma, healthcare providers and large national genomics projects have also shown keen interest in our technology as potential customers. Commercialisation will proceed in 3-steps: 1) a central data production site for early adopters, on instruments in-house, followed by 2) revenue per data subscription on rented/leased instruments placed externally, finally overtaken by 3) a growing instrument sales + consumables revenue stream.

Fields of science (EuroSciVoc)

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Keywords

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Programme(s)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

HORIZON-EIC-ACC - HORIZON EIC Accelerator

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) HORIZON-EIC-2025-ACCELERATOR-02

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Coordinator

SINGLE TECHNOLOGIES AB
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 2 496 494,88
Address
SANKT ERIKSGATAN 48F
112 34 Stockholm
Sweden

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SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
Östra Sverige Stockholm Stockholms län
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Links
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

No data
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