Objective Harnessing the capacity of pluripotent stem cells (PSCs) to produce functional cell types with precision and at scale will enable new treatment modalities for degenerative diseases. A major target for such therapies is mesodermal tissue, muscle, bone and cartilage. The goal of PluriMes is to create a bioengineering platform for directing PSCs into specific classes of mesodermal/mesenchymal progenitor. The project pulls together stem cell experts, genetic engineers, developmental biologists, cell therapy pioneers, bioengineers and specialist SMEs in a cross-disciplinary collaborative effort. Close-working between partners will be maximised through specialist workshops, laboratory exchange schemes, regular workpackage meetings, and joint Deliverables. The concept underlying PluriMes is that refined control of early fate decisions to produce correctly specified progenitors will be obtained through confronting developmental principles with engineering logic. We aim to define ground states and resolve issues of hierarchy, heterogeneity and stability in order to provide standardised PSC and mesodermal progenitor cell populations. A toolbox of fluorescent reporters will be developed for live cell tracking. Cutting edge bioengineering approaches will be implemented for dynamic topological administration of morphogens, matrices, small molecule modulators and siRNAs in 2D and 3D contexts. Through SME involvement, culture systems will be optimised for specification and subsequent stable expansion of mesodermal progenitors. The capacity of PSC-derived mesodermal cells for maturation into skeletal muscle, bone and cartilage will be tested in vitro and in vivo using lineage reporters. Potency will then be evaluated in pre-clinical disease models. Scale up protocols will be implemented in partnership with SMEs using the latest bioreactor technologies. Finally, GMP compliance and validated applicability to clinical grade stem cells will underpin future cell therapy propositions. Fields of science engineering and technologyenvironmental biotechnologybioremediationbioreactorsmedical and health sciencesmedical biotechnologycells technologiesstem cells Programme(s) FP7-HEALTH - Specific Programme "Cooperation": Health Topic(s) HEALTH.2013.1.4-1 - Controlling differentiation and proliferation in human stem cells intended for therapeutic use Call for proposal FP7-HEALTH-2013-INNOVATION-1 See other projects for this call Funding Scheme CP-FP - Small or medium-scale focused research project Coordinator THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE EU contribution € 1 593 172,26 Address TRINITY LANE THE OLD SCHOOLS CB2 1TN Cambridge United Kingdom See on map Region East of England East Anglia Cambridgeshire CC Activity type Higher or Secondary Education Establishments Administrative Contact Renata Schaeffer (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data Participants (11) Sort alphabetically Sort by EU Contribution Expand all Collapse all UNIVERSITY COLLEGE LONDON EU contribution € 919 049,20 Address GOWER STREET WC1E 6BT LONDON See on map Activity type Higher or Secondary Education Establishments Administrative Contact Malgorzata Kielbasa (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data UNIVERSITA DEGLI STUDI DI ROMA LA SAPIENZA Italy EU contribution € 434 240,00 Address Piazzale Aldo Moro 5 00185 Roma See on map Region Centro (IT) Lazio Roma Activity type Higher or Secondary Education Establishments Administrative Contact Maria Letizia Savini (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data NCARDIA BV Netherlands EU contribution € 150 000,00 Address GALLILEIWEG 8 2333 BD Leiden See on map Region West-Nederland Zuid-Holland Agglomeratie Leiden en Bollenstreek Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Stefan Braam (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data STEMCELL TECHNOLOGIES SARL Participation ended France EU contribution € 21 327,74 Address MINIPAR POLYTEC RUE DES BERGES 40 38000 GRENOBLE See on map Region Auvergne-Rhône-Alpes Rhône-Alpes Isère Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Marie-Claude Genevay (Mrs.) Links Contact the organisation Opens in new window Total cost No data TECHNISCHE UNIVERSITAET DRESDEN Germany EU contribution € 441 340,00 Address HELMHOLTZSTRASSE 10 01069 Dresden See on map Region Sachsen Dresden Dresden, Kreisfreie Stadt Activity type Higher or Secondary Education Establishments Administrative Contact Sven Kreigenfeld (Mr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data THE UNIVERSITY OF SHEFFIELD United Kingdom EU contribution € 399 762,80 Address FIRTH COURT WESTERN BANK S10 2TN Sheffield See on map Region Yorkshire and the Humber South Yorkshire Sheffield Activity type Higher or Secondary Education Establishments Administrative Contact Joanne Watson (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data ACADEMISCH ZIEKENHUIS LEIDEN Netherlands EU contribution € 425 765,60 Address ALBINUSDREEF 2 2333 ZA Leiden See on map Region West-Nederland Zuid-Holland Agglomeratie Leiden en Bollenstreek Activity type Higher or Secondary Education Establishments Administrative Contact Liesbeth Dorama (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE Switzerland EU contribution € 402 791,60 Address BATIMENT CE 3316 STATION 1 1015 Lausanne See on map Region Schweiz/Suisse/Svizzera Région lémanique Vaud Activity type Higher or Secondary Education Establishments Administrative Contact Matthias Lutolf (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data CENTRE EUROPEEN DE RECHERCHE EN BIOLOGIE ET MEDECINE France EU contribution € 399 940,00 Address Rue Laurent Fries 1 67404 Illkirch Graffenstaden See on map Region Grand Est Alsace Bas-Rhin Activity type Research Organisations Administrative Contact Steve Brooks (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data STEMCELL TECHNOLOGIES UK LTD United Kingdom EU contribution € 734 072,00 Address BUILDING 7100 CAMBRIDGDE RESEARCH PARK BEACH DRIVE WATERBEACH CB25 9TL Cambridge See on map Region East of England East Anglia Cambridgeshire CC Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Frederic Bouffechoux (Mr.) Links Contact the organisation Opens in new window Total cost No data THE UNIVERSITY OF MANCHESTER United Kingdom EU contribution € 78 538,80 Address OXFORD ROAD M13 9PL Manchester See on map Region North West (England) Greater Manchester Manchester Activity type Higher or Secondary Education Establishments Administrative Contact Claire Faichnie (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data