Objective The objective of the proposal is to use cytogenetic methods to examine fundamental aspects of variations in radiosensitivity between individuals. The proposal exploits recent advances in using the G2 assay in lymphocytes to identify normally responding and hypersensitive individuals amongst cohorts of healthy subjects and cancer patients. Current evidence suggests that G2 sensitivity is a marker of predisposition to cancer. Suitable normal and sensitive subjects will have small skin biopsiestaken and from these fibroblast cultures will be initiated. These will provide a stock of cells that will be irradiated in vi tro and examined for induced chromosomal damage. Particular emphasis will be placed on the presence of stable aberrations detected by using the fluorescence in situ hybridisation method on mitotic preparations. The data will be examined for both quantitative and qualitative differences in chromosomal responses between individuals that may correlate with their known radiosensitivity and disease status. Programme(s) EAEC-NFS 2 - Specific research and training programme in the field of nuclear safety and safeguards, 1994-1998 Topic(s) 040102 - Repair of, and recovery from, DNA damage and radiation sensitivity Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator National Radiological Protection Board (NRPB) EU contribution No data Address Chilton OX11 0RQ Didcot United Kingdom See on map Total cost No data Participants (2) Sort alphabetically Sort by EU Contribution Expand all Collapse all Christie Hospital NHS Trust United Kingdom EU contribution No data Address Wilmslow Road Didsbury M20 9BX Manchester See on map Total cost No data FUNDACION CENTRO ONCOLOGICO DE GALICIA JOSE ANTONIO QUIROGA Y PINEYRO Spain EU contribution No data Address S/N,Avenida de Montserrat S/N 15006 LA CORUNA See on map Total cost No data