Objective Enzymes are extremely powerful natural catalysts able to perform almost any type of chemical reaction while being mild by nature and highly specific. In fact, the delicate functioning of enzymes forms the basis of every living creature. The catalytic potential of enzymes is more and more appreciated by the industry as many industrial processes rely on these sophisticated catalysts. However, the number of reactions catalyzed by enzymes is restricted as enzymes only have evolved to catalyze reactions that are physiologically relevant. Furthermore, enzymes have adapted to the direct (cellular) environment in which they have to function (e.g. operative at ambient temperature, resilient towards proteolysis, catalytic turnover rate should fit with metabolic enzyme partners). This excludes the existence of enzymes that do not fit within boundaries set by nature. It is a great challenge to go beyond these natural boundaries and develop methodologies to design ‘unnatural’ tailor-made enzymes. Ideally it should become possible to (re)design enzymes to convert pre-defined substrates. Such designer enzymes could theoretically exhibit unsurpassed catalytic properties and, obviously, will be of significant interest for industrial biotechnology. The OXYGREEN project aims at the design and construction of novel oxygenating enzymes (designer oxygenases) for the production of compounds that can be used in medicine, food and agriculture and the development of novel powerful and generic enzyme redesign tools for this purpose. The enzymes and whole-cell biocatalysts that will be developed should catalyze the specific incorporation of oxygen to afford synthesis of bioactive compounds in a selective and clean way, with minimal side products and with no use of toxic materials. For this, generic platform technologies (novel high-throughput methodology and methods for engineering dedicated host cells) will be developed that allow effective structure-inspired directed evolution of enzyme. Fields of science engineering and technologyindustrial biotechnologyagricultural sciencesagriculture, forestry, and fisheriesagriculturenatural scienceschemical sciencescatalysisbiocatalysisnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Keywords biocatalysis enzyme redesign flow cytometry high-throughput screening industrial biotechnology oxyfunctionalization oxygenase Programme(s) FP7-KBBE - Specific Programme "Cooperation": Food, Agriculture and Biotechnology Topic(s) KBBE-2007-3-2-03 - DESIGNER ENZYMES - Improved biocatalysts for bioprocesses Call for proposal FP7-KBBE-2007-1 See other projects for this call Funding Scheme CP-IP - Large-scale integrating project Coordinator RIJKSUNIVERSITEIT GRONINGEN EU contribution € 1 203 149,00 Address Broerstraat 5 9712CP Groningen Netherlands See on map Region Noord-Nederland Groningen Overig Groningen Activity type Higher or Secondary Education Establishments Administrative Contact Jan Poutsma (Mr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data Participants (13) Sort alphabetically Sort by EU Contribution Expand all Collapse all CONSTRUCTOR UNIVERSITY BREMEN GGMBH Participation ended Germany EU contribution € 121 989,00 Address CAMPUS RING 1 28725 Bremen See on map Region Bremen Bremen Bremen, Kreisfreie Stadt Activity type Higher or Secondary Education Establishments Administrative Contact Ronald Kieschnick (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data TECHNISCHE UNIVERSITAT DORTMUND Germany EU contribution € 882 950,00 Address AUGUST SCHMIDT STRASSE 4 44227 Dortmund See on map Region Nordrhein-Westfalen Arnsberg Dortmund, Kreisfreie Stadt Activity type Higher or Secondary Education Establishments Administrative Contact Detlef Niehage (Mr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data BIOINFOBANK INSTITUTE Poland EU contribution € 409 200,00 Address Limanowskiego 24/a 16 60744 POZNAN See on map Activity type Research Organisations Administrative Contact Jadwiga Borowcyk (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data TECHNISCHE UNIVERSITAET WIEN Austria EU contribution € 363 600,00 Address KARLSPLATZ 13 1040 Wien See on map Region Ostösterreich Wien Wien Activity type Higher or Secondary Education Establishments Administrative Contact Marko Mihovilovic (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data TECHNISCHE UNIVERSITAET GRAZ Austria EU contribution € 832 013,00 Address RECHBAUERSTRASSE 12 8010 Graz See on map Region Südösterreich Steiermark Graz Activity type Higher or Secondary Education Establishments Administrative Contact Anton Glieder (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data UNIVERSITA DEGLI STUDI DI PAVIA Italy EU contribution € 373 350,00 Address STRADA NUOVA 65 27100 Pavia See on map Region Nord-Ovest Lombardia Pavia Activity type Higher or Secondary Education Establishments Administrative Contact Andrea Mattevi (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data DSM RESEARCH B.V. Netherlands EU contribution € 11 892,00 Address Urmonderbaan 22 6167RD GELEEN See on map Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Johan H.M. Jansen (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data EVONIK REXIM France EU contribution € 91 528,00 Address Rue de Verdun 33 80400 HAM See on map Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Dominique Frossard (Dr.) Links Contact the organisation Opens in new window Total cost No data BIOLOG LSI FORSCHUNGS- UND BETEILIGUNGS-GMBH Germany EU contribution € 258 400,00 Address FLUGHAFENDAMM 9A 28199 BREMEN See on map Region Bremen Bremen Bremen, Kreisfreie Stadt Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Hans-Gottfried Genieser (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data ENZYSCREEN Netherlands EU contribution € 98 400,00 Address Wassenaarseweg 2333 AL LEIDEN See on map Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Wouter Duetz (Dr.) Links Contact the organisation Opens in new window Total cost No data DECHEMA GESELLSCHAFT FUR CHEMISCHETECHNIK UND BIOTECHNOLOGIE Germany EU contribution € 250 140,00 Address THEODOR HEUSS ALLEE 25 60486 Frankfurt See on map Region Hessen Darmstadt Frankfurt am Main, Kreisfreie Stadt Activity type Research Organisations Administrative Contact Jens Schrader (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data RHEINISCH-WESTFAELISCHE TECHNISCHE HOCHSCHULE AACHEN Germany EU contribution € 578 530,00 Address TEMPLERGRABEN 55 52062 Aachen See on map Region Nordrhein-Westfalen Köln Städteregion Aachen Activity type Higher or Secondary Education Establishments Administrative Contact Ernst Schmachtenberg (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data DSM INNOVATIVE SYNTHESIS BV Netherlands EU contribution € 68 108,00 Address POSTSTRAAT 1 6135 KR SITTARD See on map Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Ine Weijer Van De (Mrs.) Links Contact the organisation Opens in new window Total cost No data