Objectif THE TECHNIQUES OF DNA SEQUENCING ARE OF CENTRAL IMPORTANCE IN MOLECULAR BIOLOGY AND BIOTECHNOLOGY. DETERMINATION OF THE COMPLETE NUCLEOTIDE SEQUENCES OF LARGE MOLECULES BY THE PRESENT MANUAL METHODS IS, HOWEVER, A TEDIOUS AND TIME CONSUMING PROCESS AS ARE THE ITERATIVE MANUAL DETERMINATIONS OF RELATIVELY SHORT SEQUENCES. THE REPETITIVE NATURE OF THE TASK MAKES IT AN IDEAL SUBJECT FOR AUTOMATION. THE AUTOMATION OF THE PROCESS AIMED AT IN THE PRESENT PROPOSAL SHOULD SPEED UP THE DETERMINATION OF NUCLEOTIDE SEQUENCING FOR THE BENEFIT OR BOTH ACADEMIC AND INDUSTRIAL RESEARCH. Deoxyribonucleic acid (DNA) sequencing is an obvious candidate for automation allowing more rapid access to the genetic data encoded in very large DNA molecules, such as the human genome.Research was carried out into the production of fast and accurate instrument modules to automate aspects of sequence analysis. A novel machine was designed and built which incorporated a vision controlled robot to identify and select plaques and colonies on Petri dishes for automatic sampling and transfer of candidate material for further growth in individual containers. Electrophoresis and direct blotting were automated and a gas counter was built for directly imaging radiolabelled DNA sequences and mapping gels for genetic data abstraction.The first robot system (APSCIR) to image mixed randomly disposed arrays of biological specimens and to pick a particular set of these for distribution into an ordered arrangement was produced. Imaging hardware and software were developed jointly with a patented robot picking head to automate this process, which is recognized as a serious bottleneck in many DNA analysis procedures. The direct blotting electrophoresis system was developed into a key technology for the second generation of automatic mappers and sequencers and the gas counter capabilities were extended into the area of sequence and genetic analysis.THESE PARTS OF THE GLOBAL PROJECT WILL BE TREATED : 1. TRANSFER OF ELECTROPHORESIS DATA TO NEW MULTIWIRE PROPORTIONAL COUNTER (MWPC). WORK WILL INCLUDE THE STANDARDIZATION OF ELECTROPHORESIS CONDITIONS, OFF-LINE AND LATER ON-LINE AUTOMATIC READING OF THE GELS. 2. SOFTWARE DEVELOPMENT FOR MWPC IMAGE ANALYSIS; WITH SPECIAL ATTENTION TO PORTABILITY, LOW COST HARDWARE, DATA COMPRESSION AND ROBUSTNESS. 3. IMPROVED BIOCHEMISTRY FOR OBTAINING ORDERED SUBCLONES. DEVELOPMENT OF SIMPLIFIED PROTOCOLS ALLOWING THE CONSTRUCTION OF ORDERED SUBCLONES FROM COSMIDES WITH A MINIMUM OF MANUAL HANDLING.PREPARATION OF EXPERIMENTAL KITS PERMITTING TO SEQUENCE DIRECTLY THE PLASMID DNA AND BEING ROBUST AND RELIABLE FOR AUTOMATIC PROCEDURES. Champ scientifique natural sciencesbiological sciencesgeneticsDNAsocial sciencessociologyindustrial relationsautomationnatural sciencescomputer and information sciencessoftwaresoftware developmentnatural sciencesbiological sciencesgeneticsnucleotidesnatural scienceschemical scienceselectrochemistryelectrophoresis Programme(s) FP1-BAP - Multiannual research action programme (EEC) in the field of biotechnology (BAP), 1985-1989 Thème(s) Data not available Appel à propositions Data not available Régime de financement CSC - Cost-sharing contracts Coordinateur Christian-Albrechts-Universität Contribution de l’UE Aucune donnée Adresse Olshausen str. 40 24098 Kiel Allemagne Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée Participants (3) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire Rutherford Appleton Laboratory (RAL) Royaume-Uni Contribution de l’UE Aucune donnée Adresse Chilton OX11 0QX Didcot Voir sur la carte Coût total Aucune donnée Steinbeis-Transferzentrum System- und Softwareengineering Allemagne Contribution de l’UE Aucune donnée Adresse Reichenaustraße 81c 78467 Konstanz Voir sur la carte Coût total Aucune donnée University of Manchester Institute of Science and Technology (UMIST) Royaume-Uni Contribution de l’UE Aucune donnée Adresse Sackville Street M60 1QD Manchester Voir sur la carte Coût total Aucune donnée