Extracellular vesicles from a natural source for tailor-made nanomaterials

Informations projet

VES4US

N° de convention de subvention: 801338

DOI

10.3030/801338

Projet clôturé

Date de signature de la CE 25 Mai 2018

Date de début 1 Septembre 2018

Date de fin 28 Février 2022

Financé au titre de

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coût total

€ 2 946 303,75

Contribution de l’UE

€ 2 946 303,75

Coordonné par

CONSIGLIO NAZIONALE DELLE RICERCHE
Italy

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Livrables

Websites, patent fillings, videos etc. (2)

Website and Logo

Ves4Us Logo and Website with intranet section

Optimal culture conditions

Optimised cultivation conditions are determined

Documents, reports (10)

Gender Action Plan

The Gender Action Plan will promote equality within the framework of the project

Quality Management system

A quality plan, compatible to UNI EN ISO 9001:2015 and OECD GLP standards, for the management of procedures

Dissemination and Communication Plan
Training plan

A training plan will describe detailed actions to improve the interdisciplinary methodology and the scientific cross-fertilisation amongst the partners by training staff and students of each participant institution for targeted collaborative experimentation

Cellular response to natural source-derived EVs

The cellular response to EVs will be in depth described

Functionalization strategy report

Description of the state of the art strategy/ies to be applied for micoalgal EV functionalization

First report on outreach activities

Report on outreach activities

Second report on outreach activities

Report on outreach activities

Characterization and purification of functionalized EVs

Microfluidic technology for the characterization and purification of engineered EVs

SOPs for microalgal EVs

SOPs for EVs isolation, storage and transport

Demonstrators, pilots, prototypes (3)

Preclinical studies in rodents

The potential shorttermlongterm systemic toxicity and in vivo stability of microalgaederived EVs will be assessed in rodents

Characteristics of MiPs

MiPs will be characterised and then tested for selectively capturing EVs from the microalgae cultivation media

BubbleMumble game

The science app game BubbleMumble on microalgal EV

Open Research Data Pilot (1)

Data Management Plan

DMP will be based on the 7-point structure as described in the grant agreement

Publications

Peer reviewed articles (35)

Budding and Fission of Membrane Vesicles: A Mini Review

Auteurs: Samo Penič, Luka Mesarec, Miha Fošnarič, Lucyna Mrówczyńska, Henry Hägerstrand, Veronika Kralj-Iglič, Aleš Iglič
Publié dans: Frontiers in Physics, Numéro 8, 2020, ISSN 2296-424X
Éditeur: Frontiers in Physics
DOI: 10.3389/fphy.2020.00342

Enrichment of plasma in platelets and extracellular vesicles by the counterflow to erythrocyte settling

Auteurs: Božič, Darja, Vozel, Domen, Hočevar, Matej, Jeran, Marko, Jan, Zala, Pajnič, Manca, Pađen, Ljubiša, Iglič, Aleš, Battelino, Saba, Kralj-Iglič, Veronika
Publié dans: Platelets, 2021, ISSN 0953-7104
Éditeur: Taylor & Francis
DOI: 10.1080/09537104.2021.1961716

Correlation between adhesion strength and phase behaviour in solid-supported lipid membranes

Auteurs: N. Bibissidis, K. Betlem, G. Cordoyiannis, F. Prista-von Bonhorst, J. Goole, J. Raval, M. Daniel, W. Góźdź, A. Iglič, P. Losada-Pérez
Publié dans: Journal of Molecular Liquids, Numéro 320, 2020, Page(s) 114492, ISSN 0167-7322
Éditeur: Elsevier BV
DOI: 10.1016/j.molliq.2020.114492

Antibacterial Activity and Amphidinol Profiling of the Marine Dinoflagellate Amphidinium carterae (Subclade III)

Auteurs: Maria Elena Barone, Elliot Murphy, Rachel Parkes, Gerard T. A. Fleming, Floriana Campanile, Olivier P. Thomas, Nicolas Touzet
Publié dans: International Journal of Molecular Sciences, Numéro 22/22, 2021, Page(s) 12196, ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms222212196

Isolation of extracellular vesicles from microalgae: towards the production of sustainable and natural nanocarriers of bioactive compounds

Auteurs: Sabrina Picciotto, Maria E. Barone, David Fierli, Anita Aranyos, Giorgia Adamo, Darja Božič, Daniele P. Romancino, Christopher Stanly, Rachel Parkes, Svenja Morsbach, Samuele Raccosta, Carolina Paganini, Antonella Cusimano, Vincenzo Martorana, Rosina Noto, Rita Carrotta, Fabio Librizzi, Umberto Capasso Palmiero, Pamela Santonicola, Ales Iglič, Meiyu Gai, Laura Corcuera, Annamaria Kisslinger, El
Publié dans: Biomaterials Science, Numéro 9/8, 2021, Page(s) 2917-2930, ISSN 2047-4830
Éditeur: Royal Society of Chemistry
DOI: 10.1039/d0bm01696a

Autologous Platelet- and Extracellular Vesicle-Rich Plasma Is an Effective Treatment Modality for Chronic Postoperative Temporal Bone Cavity Inflammation: Randomized Controlled Clinical Trial

Auteurs: Domen Vozel, Darja Božič, Marko Jeran, Zala Jan,Manca Pajnič, Ljubiša Pađen,Nejc Steiner, Veronika Kralj-Iglič, and Saba Battelino
Publié dans: Front Bioeng Biotechnoly, 2021, ISSN 2296-4185
Éditeur: Frontiers Media S.A.
DOI: 10.3389/fbioe.2021.677541

A failure mode and effect analysis (FMEA)-based approach for risk assessment of scientific processes in non-regulated research laboratories

Auteurs: A. Mascia, A. M. Cirafici, A. Bongiovanni, G. Colotti, G. Lacerra, M. Di Carlo, F. A. Digilio, G. L. Liguori, A. Lanati, A. Kisslinger
Publié dans: Accreditation and Quality Assurance, 2020, ISSN 0949-1775
Éditeur: Springer Verlag
DOI: 10.1007/s00769-020-01441-9

Comparative Response of Marine Microalgae to H2O2-Induced Oxidative Stress

Auteurs: Maria Elena Barone, Rachel Parkes, Helen Herbert, Adam McDonnell, Thomas Conlon, Anita Aranyos, David Fierli, Gerard T. A. Fleming, Nicolas Touzet
Publié dans: Applied Biochemistry and Biotechnology, Numéro 193/12, 2021, Page(s) 4052-4067, ISSN 0273-2289
Éditeur: Humana Press, Inc.
DOI: 10.1007/s12010-021-03690-x

Sialylation of host proteins as targetable risk factor for COVID‐19 susceptibility and spreading: A hypothesis

Auteurs: Antonella Bongiovanni, Antonella Cusimano, Ida Annunziata, Alessandra d’Azzo
Publié dans: FASEB BioAdvances, Numéro 3/3, 2021, Page(s) 192-197, ISSN 2573-9832
Éditeur: PublishMed
DOI: 10.1096/fba.2020-00073

Light Scattering as an Easy Tool to Measure Vesicles Weight Concentration

Auteurs: Giulia Di Prima, Fabio Librizzi, Rita Carrotta
Publié dans: Membranes, Numéro 10/9, 2020, Page(s) 222, ISSN 2077-0375
Éditeur: Molecular Diversity Preservation International
DOI: 10.3390/membranes10090222

Subcellular Localization of uc.8+ as a Prognostic Biomarker in Bladder Cancer Tissue

Auteurs: Sara Terreri, Sara Mancinelli, Matteo Ferro, Maria Concetta Vitale, Sisto Perdonà, Luigi Castaldo, Vincenzo Gigantino, Vincenzo Mercadante, Rossella De Cecio, Gabriella Aquino, Marco Montella, Claudia Angelini, Eugenio Del Prete, Marianna Aprile, Angelo Ciaramella, Giovanna L. Liguori, Valerio Costa, George A. Calin, Evelina La Civita, Daniela Terracciano, Ferdinando Febbraio, and Amelia Cimmino
Publié dans: Cancers, 2021, ISSN 2072-6694
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cancers13040681

Curvature Potential Unveiled Topological Defect Attractors

Auteurs: Mesarec, Luka, Iglič, Aleš, Kralj-Iglič, Veronika, Góźdź, Wojciech, Virga, Epifanio G., Kralj, Samo
Publié dans: Crystals, 2021, ISSN 2073-4352
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cryst11050539

Nanoalgosomes: Introducing extracellular vesicles produced by microalgae

Auteurs: Giorgia Adamo, David Fierli, Daniele P. Romancino, Sabrina Picciotto, Maria E. Barone, Anita Aranyos, Darja Božič, Svenja Morsbach, Samuele Raccosta, Christopher Stanly, Carolina Paganini, Meiyu Gai, Antonella Cusimano, Vincenzo Martorana, Rosina Noto, Rita Carrotta, Fabio Librizzi, Loredana Randazzo, Rachel Parkes, Umberto Capasso Palmiero, Estella Rao, Angela Paterna, Pamela Santonicola, Ales
Publié dans: Journal of Extracellular Vesicles, Numéro 10/6, 2021, ISSN 2001-3078
Éditeur: ISEV
DOI: 10.1002/jev2.12081

The Transcription Regulator Patz1 Is Essential for Neural Stem Cell Maintenance and Proliferation

Auteurs: Sara Mancinelli, Michela Vitiello, Maria Donnini, Francesca Mantile, Giuseppe Palma, Antonio Luciano, Claudio Arra, Laura Cerchia, Giovanna Lucia Liguori and Monica Fedele
Publié dans: Frontiers in Cell and Developmental Biology, 2021, ISSN 2296-634X
Éditeur: Original Research
DOI: 10.3389/fcell.2021.657149

On the Role of Curved Membrane Nanodomains and Passive and Active Skeleton Forces in the Determination of Cell Shape and Membrane Budding

Auteurs: Luka Mesarec, Mitja Drab, Samo Penič, Veronika Kralj-Iglič, Aleš Iglič
Publié dans: International Journal of Molecular Sciences, Numéro 22/5, 2021, Page(s) 2348, ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22052348

Concentration of extracellular vesicles isolated from blood relative to the clinical pathological status of dogs with mast cell tumours

Auteurs: Metka Šimundić, Tanja Švara, Roman Štukelj, Judita L Krek, Mitja Gombač, Veronika Kralj-Iglič, Nataša Tozon
Publié dans: MEDLINE, 2021, ISSN 1476-5829
Éditeur: Blackwell Publishing Inc.
DOI: 10.1111/vco.12489

Isolation of Extracellular Vesicles From Microalgae: A Renewable and Scalable Bioprocess

Auteurs: Angela Paterna, Estella Rao, Giorgia Adamo, Samuele Raccosta, Sabrina Picciotto, Daniele Romancino, Rosina Noto, Nicolas Touzet, Antonella Bongiovanni and Mauro Manno
Publié dans: Frontiers in Bioengineering and Biotechnology, 2022, ISSN 2296-4185
Éditeur: Frontiers
DOI: 10.3389/fbioe.2022.836747

Species-specific responses in pigments and fatty acids of five freshwater chlorophytes exposed to varying cultivation conditions

Auteurs: Rachel Parkes, Maria Elena Barone, Anita Aranyos, David Fierli, Henry Koehler, Eoin Gillespie, Nicolas Touzet
Publié dans: Process Biochemistry, Numéro 112, 2022, Page(s) 35-44, ISSN 1359-5113
Éditeur: Elsevier Applied Science
DOI: 10.1016/j.procbio.2021.11.018

Green kiwifruit extracts protect motor neurons from death in a spinal muscular atrophy model in Caenorhabditis elegans

Auteurs: Nadia Mazzarella, Ivana Giangrieco, Serena Visone, Pamela Santonicola, Jannis Achenbach, Giuseppina Zampi, Maurizio Tamburrini, Elia Di Schiavi, Maria Antonietta Ciardiello
Publié dans: Food Science & Nutrition, Numéro 7/7, 2019, Page(s) 2327-2335, ISSN 2048-7177
Éditeur: Wiley
DOI: 10.1002/fsn3.1078

Viscosity of Plasma as a Key Factor in Assessment of Extracellular Vesicles by Light Scattering

Auteurs: Božič, Sitar, Junkar, Štukelj, Pajnič, Žagar, Kralj-Iglič, Kogej
Publié dans: Cells, Numéro 8/9, 2019, Page(s) 1046, ISSN 2073-4409
Éditeur: Department of Chemistry and Biochemistry, University of Ljubljana
DOI: 10.3390/cells8091046

Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Auteurs: Clotilde Théry, Kenneth W Witwer, Elena Aikawa, Maria Jose Alcaraz, Johnathon D Anderson, Ramaroson Andriantsitohaina, Anna Antoniou, Tanina Arab, Fabienne Archer, Georgia K Atkin-Smith, D Craig Ayre, Jean-Marie Bach, Daniel Bachurski, Hossein Baharvand, Leonora Balaj, Shawn Baldacchino, Natalie N Bauer, Amy A Baxter, Mary Bebawy, Carla Beckham, Apolonija Bedina Zavec, Abderrahim Benmoussa, Anna
Publié dans: Journal of Extracellular Vesicles, Numéro 7/1, 2018, Page(s) 1535750, ISSN 2001-3078
Éditeur: UK Limited
DOI: 10.1080/20013078.2018.1535750

Scalable Production and Isolation of Extracellular Vesicles: Available Sources and Lessons from Current Industrial Bioprocesses

Auteurs: Carolina Paganini, Umberto Capasso Palmiero, Gabriella Pocsfalvi, Nicolas Touzet, Antonella Bongiovanni, Paolo Arosio
Publié dans: Biotechnology Journal, 2019, Page(s) 1800528, ISSN 1860-6768
Éditeur: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/biot.201800528

Inception Mechanisms of Tunneling Nanotubes

Auteurs: Mitja Drab, David Stopar, Veronika Kralj-Iglič, Aleš Iglič
Publié dans: Cells, Numéro 8/6, 2019, Page(s) 626, ISSN 2073-4409
Éditeur: Univerza v Ljubljani
DOI: 10.3390/cells8060626

Membrane Transporters in Citrus clementina Fruit Juice-Derived Nanovesicles

Auteurs: Christopher Stanly, Maneea Moubarak, Immacolata Fiume, Lilla Turiák, Gabriella Pocsfalvi
Publié dans: International Journal of Molecular Sciences, Numéro 20/24, 2019, Page(s) 6205, ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms20246205

A bio-orthogonal functionalization strategy for site-specific coupling of antibodies on vesicle surfaces after self-assembly

Auteurs: Meiyu Gai, Johanna Simon, Ingo Lieberwirth, Volker Mailänder, Svenja Morsbach, Katharina Landfester
Publié dans: Polymer Chemistry, 2020, ISSN 1759-9954
Éditeur: Royal Society of Chemistry
DOI: 10.1039/c9py01136f

Vaccination with (1–11)E2 in alum efficiently induces an antibody response to β-amyloid without affecting brain β-amyloid load and microglia activation in 3xTg mice

Auteurs: Francesca Mantile, Angelo Capasso, Nadia Villacampa, Maria Donnini, Giovanna L. Liguori, Gabriela Constantin, Piergiuseppe De Berardinis, Michael T. Heneka, Antonella Prisco
Publié dans: Aging Clinical and Experimental Research, 2019, ISSN 1720-8319
Éditeur: Springer International Publishing
DOI: 10.1007/s40520-019-01414-0

Normal red blood cells’ shape stabilized by membrane’s in-plane ordering

Auteurs: L. Mesarec, W. Góźdź, A. Iglič, V. Kralj-Iglič, E. G. Virga, S. Kralj
Publié dans: Scientific Reports, Numéro 9/1, 2019, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-019-56128-0

Role of PA2G4P4 pseudogene in bladder cancer tumorigenesis

Auteurs: Laura Pisapia, Sara Terreri, Pasquale Barba, Marianna Mastroianni, Maria Donnini, Vincenzo Mercadante, Alessandro Palmieri, Paolo Verze, Vincenzo Mirone, Vincenzo Altieri, Gianluigi Califano, Giovanna Lucia Liguori, Maria Strazzullo, Amelia Cimmino, Giovanna Del Pozzo
Publié dans: Biology, Numéro 9/4, 2020, Page(s) 66, ISSN 2079-7737
Éditeur: Biology
DOI: 10.3390/biology9040066

Minimizing isotropic and deviatoric membrane energy – An unifying formation mechanism of different cellular membrane nanovesicle types

Auteurs: Veronika Kralj-Iglič, Gabriella Pocsfalvi, Luka Mesarec, Vid Šuštar, Henry Hägerstrand, Aleš Iglič
Publié dans: PLOS ONE, Numéro 15/12, 2020, Page(s) e0244796, ISSN 1932-6203
Éditeur: Public Library of Science
DOI: 10.1371/journal.pone.0244796

Quality Management Tools on the Stage: Old but New Allies for Rigor and Standardization of Extracellular Vesicle Studies

Auteurs: Giovanna L. Liguori, Annamaria Kisslinger
Publié dans: Frontiers in Bioengineering and Biotechnology, 2022, ISSN 2296-4185
Éditeur: Original Research
DOI: 10.3389/fbioe.2022.826252

Design of Transfections (DoT): implementation of Design of Experiments for cell transfection fine-tuning.

Auteurs: Sara Mancinelli; Andrea Turcato; Annamaria Kisslinger; Antonella Bongiovanni; Valeria Zazzu; Antonella Lanati; Giovanna L. Liguori
Publié dans: Biotechnology and Bioengineering, Numéro 1, 2021, ISSN 0006-3592
Éditeur: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.27918

Decrease in Cellular Nanovesicles Concentration in Blood of Athletes More Than 15 Hours After Marathon

Auteurs: Zala Jan, Mitja Drab, Damjana Drobne, Apolonija Bedina Zavec, Mojca Benčina, Barbara Drasler, Matej Hočevar, Judita Lea Krek, Ljubiša Pađen, Manca Pajnič, Neža Repar, Boštjan Šimunič, Roman Štukelj, Veronika Kralj-Iglič
Publié dans: International Journal of Nanomedicine, Numéro Volume 16, 2021, Page(s) 443-456, ISSN 1178-2013
Éditeur: Laboratory of Clinical Biophysics
DOI: 10.2147/ijn.s282200

Mechanical and Electrical Interaction of Biological Membranes with Nanoparticles and Nanostructured Surfaces

Auteurs: Jeel Raval, Ekaterina Gongadze, Metka Benčina, ,Ita Junkar, Niharika Rawat, Luka Mesarec,Veronika Kralj-Iglič,Wojciech Góźdź and Aleš Iglič
Publié dans: Membranes, 2021, ISSN 2077-0375
Éditeur: Molecular Diversity Preservation International
DOI: 10.3390/membranes11070533

The effect of lipid phase on liposome stability upon exposure to the mechanical stress

Auteurs: Joanna Doskocz, Paulina Dałek, Aleksander Foryś, Barbara Trzebicka, Magdalena Przybyło, Luka Mesarec, Aleš Iglič, Marek Langner
Publié dans: Biochimica et Biophysica Acta (BBA) - Biomembranes, Numéro 1862/9, 2020, Page(s) 183361, ISSN 0005-2736
Éditeur: Elsevier BV
DOI: 10.1016/j.bbamem.2020.183361

Rapid Characterization and Quantification of Extracellular Vesicles by Fluorescence‐Based Microfluidic Diffusion Sizing

Auteurs: Carolina Paganini, Britta Hettich, Marie R.G. Kopp, Adam Eördögh, Umberto Capasso Palmiero, Giorgia Adamo, Nicolas Touzet, Mauro Manno, Antonella Bongiovanni, Pablo Rivera‐Fuentes, Jean‐Christophe Leroux, Paolo Arosio
Publié dans: Advanced Healthcare Materials, 2021, Page(s) 2100021, ISSN 2192-2640
Éditeur: John Wiley and Sons Ltd
DOI: 10.1002/adhm.202100021

Droits de propriété intellectuelle

Patent (2)

EXTRACELLULAR VESICLES FROM MICROALGAE

Numéro de demande/publication: 20 20086622
Date: 2020-12-17
Demandeur(s): CONSIGLIO NAZIONALE DELLE RICERCHE

EXTRACELLULAR VESICLES FROM MICROALGAE

Numéro de demande/publication: 20 20086622
Date: 2020-12-17
Demandeur(s): INSTITUTE OF TECHNOLOGY SLIGO - ITS