Objective
We aim to identify relevant aquatic chemoautotrophic prokaryotes, which can play a key biogeochemical role with the capacity to fix CO2 independently of light. Although severely underestimated, chemoautotrophy can be widespread in natural systems and it can importantly participate in carbon cycling. Chemoautotrophs are highly diverse at the phylogenetical and metabolical levels, but our knowledge about both aspects is still very limited and will be assessed in this project. Firstly, we will identify active chemoautotrophs through Stable Isotope Probing with 13CO2. By this approach, we will label and isolate the nucleic acids of aquatic chemoautotrophs with 13C, and identify them through appropriate molecular tools. Large inserts of the isolated genomes will be analysed in fosmid clone libraries, in order to find genes that can shed light to the metabolic fashion of these organisms. This is particularly relevant in order to establish links between chemoautotrophy and other biogeochemical processes. We will focus on a specific group, Crenarchaoeta, which grows chemoautotrophically and could play a key role in nitrogen cycle in marine waters, but has not yet been studied in freshwater/brackish systems such as the ones we will sample. Finally, a survey of distribution and activity of chemoautotrophs will be done by the combination of in situ hybridization with specific probes and microautoradiography with 14CO2. This will allow us to quantify the amount of active cells of different groups in CO2 uptake along environmental gradients (transcending the freshwater-coastal waters boundary), and evaluate their biogeochemical relevance in aquatic ecosystems.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules nucleic acids
- natural sciences biological sciences ecology ecosystems
- natural sciences earth and related environmental sciences hydrology limnology
- natural sciences biological sciences genetics genomes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2007-2-1-IEF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
751 05 Uppsala
Sweden
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.