Ultramicroelectrodes for Trace Element Speciation in Molecules of Biological Interest, 1992-1994

Projektinformationen

ID Finanzhilfevereinbarung: 2429

Startdatum 1 Januar 1992

Enddatum 31 Dezember 1994

Finanziert unter

• Specific research programmes (EEC) to be implemented by the Joint Research Centre - Exploratory research -, 1988-1991

Gesamtkosten € 0,00

EU-Beitrag Keine Daten

Koordiniert durch

Joint Research Centre Geel
Belgium

Ziel

Determination and speciation of metal elements in biological samples using electrochemical methods of analysis with ultramicroelectrodes (UME).

The increasing interest of these microelectrodes is due to their remarkable characteristics allowing the extension of applications for electrochemical techniques (in vivo, small volume, low temperature, study of phenomena in a very short time- microseconds-...)
The project involves the determination and speciation of metal elements in biological samples using electrochemical methods of analysis with ultramicroelectrodes (UME). The increasing interest of these microelectrodes is due to their remarkable characteristics, allowing the extension of applications for electrochemical techniques (in vivo, smallvolume, low temperature, study of phenomena in a very short time of the order of microseconds.) A voltammetric study of the behaviour of cadmium thionein and zinc thionein using conventional electrodes has been undertaken.
Progress to end 1991

Basic research : Voltammetric study on behaviour of Cd, Zn thionein using conventional electrodes.

Detailed description of work foreseen in 1992 (expected results)

- Checking these UME using well known electrochemical systems.
- Study "in vitro" using commercial UMEs for metal-ion analysis and speciation in metallothioneins. Optimisation of experimental conditions as a function of different parameters :

Redox potentials for elements : cadnium, zinc, copper, lead, iron
Nature of electrode : platinum, gold, pyrolitic graphite, mercury film
Electrochemical method : cyclic voltammetry, differential pulse polarography, square-wave voltammetry, stripping voltammetry, chronoamperometry.

Short description of evolution of work in 1993

- Characterization and analysis of other metalloproteins
- Satisfying the high interest of pharmaceutical industry in specific method developments
- Applications "in vivo".

Programm/Programme

• JRC-EXPLORES 1C - Specific research programmes (EEC) to be implemented by the Joint Research Centre - Exploratory research -, 1988-1991

Thema/Themen

Data not available

Aufforderung zur Vorschlagseinreichung

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Finanzierungsplan

Data not available

Koordinator

Joint Research Centre Geel

• EU-Beitrag: Keine Daten
• Adresse: Steenweg op Retie ; 2240 GEEL ; Belgien
• Gesamtkosten: Keine Daten