Obiettivo The overall objectives of the INTEGRA project is to develop new genetic engineering tools that will permit targeted integration of a transgene into the genome of the desired eukaryotic organism. This project will allow the easy and rapid generation of transgenic knock out or knock in small and large animals without the need of embryonic stem cells. This will have visible repercussions on fundamental and applied research particularly in pharmaceutics agronomy and food sciences.At the same time it will strengthen European research in the field of gene therapy with the development of safe viral vectors based on non integrative or targeted integration vectors adequate for clinical use. We plan to meet the objectives by two alternative means. The first one will combine the use of a lentiviral episomal vector with a phage site specific integration system to direct the integration of the transgene into a unique locus in the host genome. The alternative method will use lentiviral vectors for which the integrase specificity will be modified HIV integrases will be generated whose DNA binding domain will be replaced by a synthetic DNA binding domain able to recognize any given DNA sequence of 9 or 18 base pairs. Such specific integrase will be incorporated into a gene transfer vector system that will allow the genetic modification of somatic cells and zygotes.These technologies will be used by the partners to generate transgenic livestock with modified traits improved growth traits disease resistance and to generate sensor animals to monitor contamination of livestock by toxic agents. On the other hand gene therapy preclinical studies will be performed using the secured vectors Specific ethical and societal implications of the project will be anticipated through the participation of European stakeholders. The overall INTEGRA project clearly cuts across the Thematic Priority 1 and 5 and the Science and Society programme of FP6. Campo scientifico agricultural sciencesagriculture, forestry, and fisheriesagricultureagronomy Parole chiave Knock Knock out livestock and ethics food food safety livestock out recombinase safety sciences site social social sciences and ethics specific specific recombinase targeted targeted site Programma(i) FP6-POLICIES - Policy support: Specific activities covering wider field of research under the Focusing and Integrating Community Research programme 2002-2006. Argomento(i) NEST-2004-ADV - Adventure activities Invito a presentare proposte FP6-2004-NEST-C-1 Vedi altri progetti per questo bando Meccanismo di finanziamento STREP - Specific Targeted Research Project Coordinatore CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE Contributo UE Nessun dato Indirizzo 3 rue Michel Ange PARIS Francia Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato Partecipanti (8) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto CENTRE DE REGULACIO GENOMICA Spagna Contributo UE Nessun dato Indirizzo Passeig Maritim, 37-49 BARCELONA Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato ROSLIN INSTITUTE Regno Unito Contributo UE Nessun dato Indirizzo Roslin Midlothian ROSLIN Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato THE UNIVERSITY OF COPENHAGEN Danimarca Contributo UE Nessun dato Indirizzo Norregade 10 COPENHAGEN Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato FONDATION ASILE DES AVEUGLES Svizzera Contributo UE Nessun dato Indirizzo 15, av. de France LAUSANNE Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato THE UNIVERSITY OF EDINBURGH Regno Unito Contributo UE Nessun dato Indirizzo Old College, South Bridge EDINBURGH Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE Francia Contributo UE Nessun dato Indirizzo 101 rue de Tolbiac PARIS Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato UNIVERSITÉ DE STRASBOURG Francia Contributo UE Nessun dato Indirizzo Blaise Pascal 4 STRASBOURG Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato UNIVERSITÉ PARIS SUD XI Francia Contributo UE Nessun dato Indirizzo 15 rue Georges Clémenceau ORSAY Mostra sulla mappa Collegamenti Sito web Opens in new window Costo totale Nessun dato