RNA-protein Nanostructures for Synthetic Biology

Información del proyecto

RNA ORIGAMI

Identificador del acuerdo de subvención: 683305

DOI

10.3030/683305

Proyecto cerrado

Fecha de la firma de la CE 29 Marzo 2016

Fecha de inicio 1 Abril 2016

Fecha de finalización 31 Marzo 2021

Financiado con arreglo a

• EXCELLENT SCIENCE - European Research Council (ERC)

Coste total

€ 1 999 935,00

Aportación de la UE

€ 1 999 935,00

Coordinado por

AARHUS UNIVERSITET
Denmark

Periodic Reporting for period 4 - RNA ORIGAMI (RNA-protein Nanostructures for Synthetic Biology)

Período documentado: 2020-10-01 hasta 2021-03-31

Resumen del contexto y de los objetivos generales del proyecto

The RNA ORIGAMI project aimed at rationally designing RNA nanostructures that can be genetically expressed and function as scaffolds for controlling e.g. protein function in cells. This technology can potentially be used for a wide range of applications in synthetic biology such as production of cheaper drugs, sustainable fuel production, efficient diagnosis, and RNA medicine. The main objectives of the project were to develop design software, to characterize designed RNA scaffolds, to develop RNA sensor devices, and to demonstrate proof-of-concept applications in synthetic biology. The project succeeded in developing software tools to improve size, yield and functionalization of RNA nanostructures, demonstrated novel RNA sensors, and applied RNA nanostructures in metabolic engineering.

Trabajo realizado desde el comienzo del proyecto hasta el final del período abarcado por el informe y los principales resultados hasta la fecha

The RNA ORIGAMI project initially developed software for the rapid design of RNA origami structures and used biophysical characterization techniques to verify the folding of the designed structures. The software has been made publicly available in a code repository and as a webserver and has been used for obtain several research results: (1) RNA sensors were developed based on Förster Resonance Energy Transfer (FRET) between fluorescent aptamers, (2) RNA therapeutic particles were developed to control blood coagulation, (3) RNA scaffolds were used to stabilize functional motifs in E. coli cells, (4) RNA scaffolds were used to control enzymatic reactions in E. coli and yeast. These core results are being exploited for RNA medicine and metabolic engineering and have been or are in the process of being published in the scientific literature.

Avances que van más allá del estado de la técnica e impacto potencial esperado (incluida la repercusión socioeconómica y las implicaciones sociales más amplias del proyecto hasta la fecha)

The RNA ORIGAMI project has improved our ability to construct genetically expressible RNA nanostructures. We have improving folding yield, reached construct sizes of 2000 bases in length, and demonstrated precise scaffolding of proteins and small molecules. The development of an aptamer-based FRET system has provided a new way to develop genetically expressible sensors systems and to measure conformational changes in cells. RNA origami has been demonstrated as a powerful tool in synthetic biology by stabilizing functional motifs in cells, by regulating protein expression, and by controlling enzymatic pathways.

RNA origami folding