Program for Innovative Global Prevention of Streptococcus suis.

Projektinformationen

PIGSs

ID Finanzhilfevereinbarung: 727966

DOI

10.3030/727966

Projekt abgeschlossen

EK-Unterschriftsdatum 16 Mai 2017

Startdatum 1 Juni 2017

Enddatum 31 August 2022

Finanziert unter

• SOCIETAL CHALLENGES - Food security, sustainable agriculture and forestry, marine, maritime and inland water research, and the bioeconomy

Gesamtkosten

€ 4 998 103,75

EU-Beitrag

€ 4 998 103,75

Koordiniert durch

WAGENINGEN UNIVERSITY
Netherlands

CORDIS bietet Links zu öffentlichen Ergebnissen und Veröffentlichungen von HORIZONT-Projekten.

Links zu Ergebnissen und Veröffentlichungen von RP7-Projekten sowie Links zu einigen Typen spezifischer Ergebnisse wie Datensätzen und Software werden dynamisch von OpenAIRE abgerufen.

Leistungen

Documents, reports (21)

Report on effects of PRRSV infection on S. suis interaction in lung slices

Report on effects of PRRSV infection on S. suis interaction in precision cut lung slices

Report on cost of S. suis infection at the farm level

Report on cost of S suis infection at the farm level

Report on the ‘cornerstone’ species that are predicted to stabilize microbial communities in healthy piglets

Report on ‘cornerstone’ taxa that are predicted to stabilize microbial communities in healthy piglets and species that might protect piglets against S. suis and other pathogens including viruses based on co-occurrence networks

Report on the impact of PRRSV in vivo infection on S. suis co-infection

Report on the impact of PRRSV in vivo infection on S suis coinfection

Report on retrospective data on incidence of S. suis infection in humans in the partner countries
Report on estimated economic losses due to S. suis infection per country

Report on estimated economic losses due to S suis infection per country

Identification of genes required for fitness under in vivo mimicking conditions by Tn-Seq

TnSeq technique applied to conditions mimicking those in vivo to identify conditionally essential genes

Report on virulence of selected mutants in porcine infection models.

2 most relevant gene mutants verified in virulence in porcine infection model using data from virulence studies in vitro

Report on identified risk factors for S. suis infection of pigs

Report on identified risk factors for S suis infection of pigs

Report on the effects of SIV on bacterial adherence and colonization at respiratory epithelia using PCLS and ALI cultures of airway epithelial cells
Report on precise definition of an immune status conferring colonisation resistance against S. suis

Report on precise definition of an immune status conferring colonisation resistance against S suis

Report on the precise definition of porcine immune responses to virulent and non-virulent S. suis

Report on the precise definition of porcine immune responses to virulent and nonvirulent S suis

Report on the burden of (zoonotic) disease estimates in humans in the EU

Report on the burden of zoonotic disease estimates in humans in the EU

Report on bacterial genes involved in S. suis/SIV interactions in epithelial cells
RReport on S. suis gene expression in blood/CSF vs artificial culture media

Report on gene expression of at least one strain of S. suis under conditions mimicking those in vivo. Information utilised in vaccine and virulence studies.

Report on the deep analysis of the microbiota composition in healthy and S. suis-diseased piglets

characterise diversity, composition and functional capacities of microbiota and key abundant taxa associated with healthy and disease-associated microbiota communities.

Report of retrospective data on incidence of S. suis infection in pigs in the partner countries
Report on virulence of mutants

Report on the growth and virulence of 4 to 20 mutants in vitro

Report on host genes important for the innate immune response against S. suis

Report on host genes important for the innate immune response against S suis

Report on the burden of disease estimates in pigs for the partner countries
Report on PRRSV /SIV on innate immunity of the lower respiratory tract

Report on PRRSV SIV on innate immunity of the lower respiratory tract

Demonstrators, pilots, prototypes (2)

Standardised diagnostic tests for identification of disease-causing and non-disease causing strains of S. suis in pure culture and in pig samples
Definition of a new generation probiotic product to stabilize the microbiota, support immune system and prevent/reduce risk of S. suis disease in pigs

Definition of a new generation probiotic product comprising 1 or more commensal strains to stabilize the microbiota, support immune system and prevent/reduce risk of S. suis disease in pigs

Websites, patent fillings, videos etc. (6)

Establishment of Project website and information share point
Public Science Event 1

Presentations to the public at National Science Events or at open days or local science events for the general public

Public Science Event 3

1 or 2 presentations to the public at National Science Events or at open days or local science events for the general pub

Public Science Event 4

1 or 2 presentations to the public at National Science Events or at open days or local science events for the general public

Public Science Event 2

1 or 2 presentations to the public at National Science Events or at open days or local science events for the general pub

Public Science Event 5

1 or 2 presentations to the public at National Science Events or at open days or local science events for the general pub

Other (7)

Genome database containing core genome, meta data, virulence, serotype, MLST, antibiotic resistance

Bioinformatic analyses of S. suis genome sequences in database

Molecular epidemiological analysis

Molecular epidemiological analysis of S. suis for input into WP6

Collection of isolates

Multinational collection of S. suis isolates for sequencing

Characterisation of Type I and Type III phase variable systems

Characterisation of phase variable restriction modification systems

Mutants constructed in selected candidate virulence genes in S. suis strains

Mutants constructed in selected candidate virulence genes made in two virulent strains of S. suis.

Sequencing of isolate collection

Genome sequencing of around 90% S. suis isolates completed

Final workshop for stakeholders

One year before the end of the project we will broadly advertise a Final PIGSs Workshop to present the project achievements to the most important stakeholders. The workshop will be an opportunity for politicians, decision makers, scientists and professionals to network, exchange knowledge and discuss the economic, environmental, social, and industrial impacts.

Veröffentlichungen

Peer reviewed articles (11)

Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain

Autoren: Neila-Ibáñez, Carlos; Brogaard, Louise; Pailler-García, Lola; Martínez, Jorge; Segalés, Joaquim; Segura, Mariela; Heegaard, Peter M. H.; Aragon, Virginia
Veröffentlicht in: Neila-Ibanez , C , Brogaard , L , Pailler-Garcia , L , Martinez , J , Segales , J , Segura , M , Heegaard , P M H & Aragon , V 2021 , ' Piglet innate immune response to Streptococcus suis colonization is modulated by the virulence of the strain ' , Veterinary Research , vol. 52 , 145 . https://doi.org/10.1186/s13567-021-01013-w, Ausgabe 15, 2021, ISSN 0928-4249
Herausgeber: BioMed Central
DOI: 10.1186/s13567-021-01013-w

Feed additives for the control of post-weaning Streptococcus suis disease and the effect on the faecal and nasal microbiota

Autoren: Florencia Correa-Fiz, Carlos Neila-Ibáñez, Sergio López-Soria, Sebastian Napp, Blanca Martinez, Laia Sobrevia, Simon Tibble, Virginia Aragon, Lourdes Migura-Garcia
Veröffentlicht in: Scientific Reports, Ausgabe 10/1, 2020, ISSN 2045-2322
Herausgeber: Nature Publishing Group
DOI: 10.1038/s41598-020-77313-6

Stochastic Assessment of the Economic Impact of Streptococcus suis-Associated Disease in German, Dutch and Spanish Swine Farms

Autoren: Carlos Neila-Ibáñez1,2, Jordi Casal1,2,3, Isabel Hennig-Pauka4, Norbert Stockhofe-Zurwieden5, Marcelo Gottschalk6, Lourdes Migura-García1,2, LolaPailler-García1,2 andSebastiánNapp1,2*
Veröffentlicht in: Frontiers in Veterinary Science, 2021, ISSN 2297-1769
Herausgeber: Frontiers
DOI: 10.3389/fvets.2021.676002

Streptococcus suis Induces Expression of Cyclooxygenase-2 in Porcine Lung Tissue

Autoren: Muriel Dresen; Josephine Schenk; Yenehiwot Berhanu Weldearegay; Désirée Vötsch; Wolfgang Baumgärtner; Peter Valentin-Weigand; Andreas Nerlich
Veröffentlicht in: Microorganisms, Ausgabe 1, 2021, ISSN 2076-2607
Herausgeber: MDPI
DOI: 10.17169/refubium-30219

Identification of Streptococcus suis putative zoonotic virulence factors: a systematic review and genomic meta-analysis

Autoren: Thomas J. Roodsant, Boas C.L. van der Putten, Sara M. Tamminga, Constance Schultsz, Kees C.H. van der Ark
Veröffentlicht in: Virulence, 2021, Seite(n) 0-0, ISSN 2150-5594
Herausgeber: Landes Bioscience
DOI: 10.1080/21505594.2021.1985760

Active Human and Porcine Serum Induce Competence for Genetic Transformation in the Emerging Zoonotic Pathogen Streptococcus suis

Autoren: Maria Laura Ferrando, Alex Gussak, Saskia Mentink, Marcela Fernandez Gutierrez, Peter van Baarlen, Jerry Mark Wells
Veröffentlicht in: Pathogens, Ausgabe 10/2, 2021, Seite(n) 156, ISSN 2076-0817
Herausgeber: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pathogens10020156

Identification of conditionally essential genes for Streptococcus suis infection in pigs

Autoren: Jesús Arenas, Aldert Zomer, Jose Harders-Westerveen, Hester J. Bootsma, Marien I. De Jonge, Norbert Stockhofe-Zurwieden, Hilde E. Smith, Astrid De Greeff
Veröffentlicht in: Virulence, Ausgabe 11/1, 2020, Seite(n) 446-464, ISSN 2150-5594
Herausgeber: Landes Bioscience
DOI: 10.1080/21505594.2020.1764173

Five Complete Genome Sequences Spanning the Dutch Streptococcus suis Serotype 2 and Serotype 9 Populations

Autoren: Boas C. L. van der Putten, Thomas J. Roodsant, Martin A. Haagmans, Constance Schultsz, Kees C. H. van der Ark
Veröffentlicht in: Microbiology Resource Announcements, Ausgabe 9/6, 2020, ISSN 2576-098X
Herausgeber: ASM Journals
DOI: 10.1128/mra.01439-19

Viral Coinfection Replaces Effects of Suilysin on Streptococcus suis Adherence to and Invasion of Respiratory Epithelial Cells Grown under Air-Liquid Interface Conditions

Autoren: Fandan Meng, Jie Tong, Désirée Vötsch, Ju-Yi Peng, Xuehui Cai, Maren Willenborg, Georg Herrler, Nai-Huei Wu, Peter Valentin-Weigand
Veröffentlicht in: Infection and Immunity, Ausgabe 87/8, 2019, ISSN 0019-9567
Herausgeber: American Society for Microbiology
DOI: 10.1128/iai.00350-19

Environmental and maternal factors shaping tonsillar microbiota development in piglets

Autoren: Fredriksen S, Guan X, Boekhorst J, Molist F, van Baarlen P, Wells JM
Veröffentlicht in: BMC, 2022, ISSN 1471-2180
Herausgeber: BioMed Central
DOI: 10.1186/s12866-022-02625-8

Identification and characterization of the cell division protein MapZ from Streptococcus suis.

Autoren: Muriel Dresen; Manfred Rohde; Jesús Arenas; Astrid de Greeff; Andreas Nerlich; Andreas Nerlich; Peter Valentin-Weigand
Veröffentlicht in: MicrobiologyOpen, 10(5), Ausgabe 17, 2021, ISSN 2045-8827
Herausgeber: John Wiley and Sons Inc.
DOI: 10.1002/mbo3.1234