Super-sensitive detection of Alzheimer’s disease biomarkers in plasma by an innovative droplet split-and-stack approach

Informazioni relative al progetto

SensApp

ID dell’accordo di sovvenzione: 829104

DOI

10.3030/829104

Progetto chiuso

Data della firma CE 17 Dicembre 2018

Data di avvio 1 Gennaio 2019

Data di completamento 30 Giugno 2022

Finanziato da

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Costo totale

€ 3 287 562,50

Contributo UE

€ 3 287 562,50

Coordinato da

CONSIGLIO NAZIONALE DELLE RICERCHE
Italy

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Demonstrators, pilots, prototypes (3)

Integrated system for calibration and testing
First prototypes of climate chamber module and liquid handling module and specification for the automated system

Realization of the first prototypes of climate chamber module and liquid handling module and specification for the automated system

First prototype of the three individual layers for successive full integration

Realization of the first prototype of the three individual layers for successive full integration of the super-sensor.

Websites, patent fillings, videos etc. (3)

Logo and webpage

Realization of the logo of the Project and of a dedicated website with domain .eu in the address.

Flyers with project targets and first results and promotional movie

Flyers which describe the project targets and the first results achieved. Movie which explains the aims of the Project and its impact in order to disseminate as best as possible the research activities.

International Symposium

Organization of an International Symposium involving the main Universities and Research Centers and Companies working in the field of early diagnosis in neurodegenerative diseases but also on fields regarding microscale fluid handling and immunoreactions at sub-microliter levels.

Documents, reports (5)

Final plan for dissemination and exploitation

Report on the final plan for dissemination and exploitation of the results

Technical/scientific review meeting 1 documents

Document delivered as first report with a summary on the work in progress and on the use of resources including those regarding the dissemination activities

Report on sensitivity and specificity for tau

Report containing the data obtained for the sensitivity and the specificity of the supersensor for the biomarker tau

Report on sensitivity and specificity for Abeta1-42

Report containing the data concerning the results on the sensitivity and specificity of the supersensor for the biomarker Abeta142

Report on sensitivity and specificity for P-tau

Report document containing the data obtained for the sensitivity and the specificity of the supersensor for the biomarker Ptau

Pubblicazioni

Peer reviewed articles (11)

High‐Rate Accumulation of Tiny Aqueous Droplets Using a Pyroelectrohydrodynamic Jet System

Autori: Volodymyr Tkachenko, Reinhard Schwödiauer, Romina Rega, Simona Itri, Martina Mugnano, Danila del Giudice, Heidi Ottevaere, Yunfeng Nie, Pietro Ferraro, Simonetta Grilli
Pubblicato in: Advanced Engineering Materials, 2021, Pagina/e 2100756, ISSN 1438-1656
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/adem.202100756

Direct printing of gold nanospheres from colloidal solutions by pyro-electrohydrodynamic jet allows hypersensitive SERS sensing

Autori: Marianna Pannico, Pellegrino Musto, Romina Rega, Veronica Vespini, Simonetta Grilli, Pietro Ferraro
Pubblicato in: Applied Surface Science, Numero 531, 2020, Pagina/e 147393, ISSN 0169-4332
Editore: Elsevier BV
DOI: 10.1016/j.apsusc.2020.147393

Miniaturized, high numerical aperture confocal fluorescence detection enhanced with pyroelectric droplet accumulation for sub-attomole analyte diagnosis

Autori: YUNFENG NIE, UUSITALO SANNA, TEEMU SIPOLA, ANNUKKA KOKKONEN, INKA PÄKKILÄ, JUHA SUMEN, KATARIINA RAHKAMAA-TOLONEN, VOLODYMYR TKACHENKO, VERONICA VESPINI, SARA COPPOLA, PIETRO FERRARO, SIMONETTA GRILLI, HEIDI OTTEVAERE
Pubblicato in: Biomedical Optics Express, 2023, ISSN 2156-7085
Editore: The Optical Society
DOI: 10.1364/boe.504757

High-accuracy characterization of pyroelectric materials: A noncontact method based on surface potential measurements

Autori: Reinhard Schwödiauer, Simona Bauer-Gogonea, Martin Kaltenbrunner, Volodymyr Tkachenko, Simonetta Grilli
Pubblicato in: JOURNAL OF ADVANCED DIELECTRICS, 2023, ISSN 2010-1368
Editore: World Scientific
DOI: 10.1142/s2010135x23410023

Rapid Formation of Self‐Supporting Polydimethylsiloxane Sheets with Periodic Clusters of Embedded Nickel Nanoparticles

Autori: Romina Rega, Juan Francisco Muñoz Martinez, Vito Pagliarulo, Angela Longo, Mariano Palomba, Gianfranco Carotenuto, Pietro Ferraro, Simonetta Grilli
Pubblicato in: Advanced Materials Interfaces, 2021, Pagina/e 2002216, ISSN 2196-7350
Editore: Wiley-VCH GmbH
DOI: 10.1002/admi.202002216

Cellular Uptake of Mildly Oxidized Nanographene for Drug-Delivery Applications

Autori: M. Mugnano, G. C. Lama, R. Castaldo, V. Marchesano, F. Merola, D. del Giudice, A. Calabuig, G. Gentile, V. Ambrogi, P. Cerruti, P. Memmolo, V. Pagliarulo, P. Ferraro, S. Grilli
Pubblicato in: ACS Applied Nano Materials, Numero 3/1, 2019, Pagina/e 428-439, ISSN 2574-0970
Editore: ACS Publications
DOI: 10.1021/acsanm.9b02035

Detecting Collagen Molecules at Picogram Level through Electric Field-Induced Accumulation

Autori: Romina Rega, Martina Mugnano, Emilia Oleandro, Volodymyr Tkachenko, Danila del Giudice, Gianluca Bagnato, Pietro Ferraro, Simonetta Grilli, Sebastiano Gangemi
Pubblicato in: Sensors, Numero 20/12, 2020, Pagina/e 3567, ISSN 1424-8220
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/s20123567

Quantitative determination of rapid biomass formation on pyro-electrifiedpolymer sheets

Autori: Emilia Oleandro, Romina Rega, Martina Mugnano, Filomena Nazzaro, Pietro Ferraro, Simonetta Grilli
Pubblicato in: Biofilm, Numero 3, 2021, Pagina/e 100040, ISSN 2590-2075
Editore: Elsevier
DOI: 10.1016/j.bioflm.2020.100040

Highly sensitive detection of the neurodegenerative biomarker Tau by using the concentration effect of the pyro-electrohydrodynamic jetting

Autori: Concetta Di Natale, Sara Coppola, Veronica Vespini, Volodymyr Tkachenko, Simone Russo, Giuseppina Luciani, Giuseppe Vitiello, Francesca Ferranti, Silvia Mari, Pietro Ferraro, Pier Luca Maffettone, Simonetta Grilli
Pubblicato in: Biosensors and bioelectronics, 2024, ISSN 0956-5663
Editore: Pergamon Press Ltd.
DOI: 10.1016/j.bios.2024.116234

Pyro-Electrification of Freestanding Polymer Sheets: A New Tool for Cation-Free Manipulation of Cell Adhesion in vitro

Autori: Romina Rega, Oriella Gennari, Laura Mecozzi, Vito Pagliarulo, Martina Mugnano, Emilia Oleandro, Filomena Nazzaro, Pietro Ferraro, Simonetta Grilli
Pubblicato in: Frontiers in Chemistry, Numero 7, 2019, ISSN 2296-2646
Editore: Frontiers
DOI: 10.3389/fchem.2019.00429

A pin-based pyro-electrohydrodynamic jet sensor for tuning theaccumulation of biomolecules down to sub-picogram level detection

Autori: The detection of low abundant biomarkers is of great interest in different clinical cases, such as the early diagnosis of Alzheimer's disease. Here we present what we call pin-based pyro-electrohydrodynamic jet (p-jet) sensor that can linearly tune the accumulation rate of biomolecules in tiny droplets. In this newly revealed pinbased configuration we demonstrate the ability to detect biomolecules
Pubblicato in: Sensing and Bio-Sensing Research, Numero 22141804, 2022, ISSN 2214-1804
Editore: Elsevier BV
DOI: 10.1016/j.sbsr.2022.100536

Thesis and dissertations (2)

Dispensing pico droplets by pyroelectrohydrodynamic jetting: simulations and experimental results

Autori: Anna Faraone
Pubblicato in: 2019
Editore: Zendo
DOI: 10.5281/zenodo.3530462

Numerical simulations of droplet dynamics through an orifice – pin system for pyroelectrodynamic shooting

Autori: Pentella Domenico
Pubblicato in: 2020
Editore: Zenodo
DOI: 10.5281/zenodo.4767244

Conference proceedings (2)

SensApp: a FET-open project for developing a supersensor able to detect Alzheimer's disease biomarkers in blood

Autori: Romina Rega, Martina Mugnano, Danila del Giudice, Simona Itri, Volodymyr Tkachenko, Veronica Vespini, Sara Coppola, Pietro Ferraro, Heidi Ottevaere, Yunfeng Nie, Sanna Uusitalo, Reinhard Schwoediauer, Martin Kaltenbrunner, Markku Känsäkoski, Emanuela Mazzon, Pier Luca Maffettone, Gaetano D'Avino, Simonetta Grilli
Pubblicato in: Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V, 2021, Pagina/e 58, ISBN 9781510644076
Editore: SPIE
DOI: 10.1117/12.2596966

Highly sensitive detection of low abundant molecules by pyro-electrohydro-dynamic jetting

Autori: ROMINA REGA, Martina Mugnano, Danila Del Giudice, Simona Itri, Volodymyr Tkachenko, veronica Vespini, Sara Coppola, Emanuela Mazzon, Emilia Oleandro, Pietro Ferraro, Simonetta Grilli
Pubblicato in: Biophotonics in Point-of-Care, 2020, Pagina/e 38, ISBN 9781510634954
Editore: SPIE
DOI: 10.1117/12.2564859