General-purpose virus-neutralizing engulfing shells with modular target-specificity

Risultati finali

Public website: the website will present the partners, the overall research goals and objectives and regular updates about achievements (publications, patents), ongoing events, symposium and related activities from other entities around the world that are of interest.

VIROFIGHT symposium

VIROFIGHT symposium:A stakeholder symposium will be organised to promote the results including the broader science and society implications of the project outputs. In this symposium, the consortium will make potential users aware of the technology and achievements especially in the industry and ensure exploitation and dissemination to a broad public.

VIROFIGHT communication kit

VIROFIGHT communication kit: This deliverable covers project flyer, slide show presentation (powerpoint, keynote) and poster.

Report on polymerized nucleic acids as engulfing shells on viruses

Report on polymerized nucleic acids as engulfing shells on virusesTriangular subunits for prefabricated shells furnished with virusspecific aptamers such that shell assembly will be templated by a virus surface AU will design small nucleic acid tiles featuring multiple aptamers that bind a repetitive subunit in the viral surface to yield 24 multivalent 46 nm shell building blocks with sticky DNA linkers stabilized into duplexes by the avidity effect from the viral surface CryoEM and electrophoretic mobility shift assays will be used to assess particle shape integrity and proper basic functionality Designs will be iteratively improved Report will cover results of these actions

Characterization of sugar-conjugated aptamers directed towards influenza virus with neutralization potential

Selection of sugar modified, serum stable aptamers directed to influenza proteins. Further multimerization of aptamers in multivalent scaffolds and nanoshells.

Generation of non-infectious and infectious virus particles from non-enveloped viruses

Generation of noninfectious and infectious virus particles from nonenveloped virusesThis deliverable comprises solutions containing virus particles to be handed over to partners of the consortium for further experiments

Generation of influenza A viruses as exemplary enveloped virus

Production of several influenza strains as alternative exemplary enveloped virus: This deliverable comprises solutions containing the influenza A viruses for neutralization assays (UREG) and to be handed over to partners of the consortium for further experiments.

Report on library of prototype DNA origami shell designs

Report on library of prototype DNA origami shell designs: This report will contain blueprints, 3D models, and DNA sequences needed to build the shells design variants. In addition, wherever applicable, experimental data obtained on the designs will be added and discussed, comprising e.g. cryo EM images, electrophoretic mobility shift analysis. etc

Generation of VLPs and LVs pseudotyped with surface proteins of enveloped viruses

Generation of VLPs and LVs pseudotyped with surface proteins of enveloped viruses: This deliverable comprises solutions containing the virus-like particles and lentiviral pseudotyped particles to be handed over to partners of the consortium for further experiments.

Report on assembly procedures for prefabricated DNA origami shells

Report on assembly procedures for prefabricated DNA origami shellsIn this report experimental procedures will be described leading to successful assembly of prefabricated DNA origami shells including thermal annealing conditions reaction mixture conditions purification steps and quality control procedures In addition troubleshooting measures and inluence of environmental conditions such as temperature or cation content on assembly will be described

Dissemination and communication plan

Dissemination and communication planA detailed communication and dissemination plan covering all communication and dissemination activities required during the project and adapted to the different target audiences and objectives of the communication or dissemination measures

Neutralization potency / breadth scores for shells binding to virus samples

Neutralization potency / breadth scores for shells binding to virus samples:This deliverable provides results from neutralization experiments using shells and on-virus-assembling shells to provide quantitative feedback on neutralization performance, to be used for refining designs.

Generation of hepatitis B viruses as exemplary enveloped virus

Generation of hepatitis B viruses as exemplary enveloped virusThis deliverable comprises solutions containing the hepatitis B viruses to be handed over to partners of the consortium for further experiments

Exploitation Innovation Plan

An initial exploitation innovation plan will be presented in M12 An updated version will be created in M36

Design details and protocols for modular swap out of virus recognizing moieties

Design details and protocols for modular swap out of virus recognizing moietiesSubsets of shellforming DNA oligonucleotides will be modified with terminal extensions pointing into the shell interior The sequences of the extensions will first be dummies to deal with and remove potential aggregation issues induced through Velcro effects Iterative refinements will be made so that arbitrary sequences can be attached in tunable decoration density and geometry in a plug and play fashion without affecting the shell quality and functionality AU will deliver virusbinding candidate DNA aptamer sequences to be included as direct terminal extensions or sticky linkers

Data Management Plan

Pubblicazioni

A DNA turbine powered by a transmembrane potential across a nanopore

Autori: Xin Shi, Anna-Katharina Pumm, Christopher Maffeo, Fabian Kohler, Elija Feigl, Wenxuan Zhao, Daniel Verschueren, Ramin Golestanian, Aleksei Aksimentiev, Hendrik Dietz, Cees Dekker
Pubblicato in: Nature Nanotechnology, Numero 19, 2024, Pagina/e 338-344, ISSN 1748-3387
Editore: Nature Publishing Group
DOI: 10.1038/s41565-023-01527-8

Computationally designed Spike antigens induce neutralising responses against the breadth of SARS-COV-2 variants

Autori: Sneha Vishwanath, George William Carnell, Martina Billmeier, Luis Ohlendorf, Patrick Neckermann, Benedikt Asbach, Charlotte George, Maria Suau Sans, Andrew Chan, Joey Olivier, Angalee Nadesalingam, Sebastian Einhauser, Nigel Temperton, Diego Cantoni, Joe Grove, Ingo Jordan, Volker Sandig, Paul Tonks, Johannes Geiger, Christian Dohmen, Verena Mummert, Anne Rosalind Samuel, Christian Plank, Rebecca
Pubblicato in: npj Vaccines, Numero 9, 2024, ISSN 2059-0105
Editore: Nature portfolio
DOI: 10.1038/s41541-024-00950-9

Comparable cellular and humoral immunity upon homologous and heterologous COVID-19 vaccination regimens in kidney transplant recipients

Autori: Nina Körber, Christopher Holzmann-Littig, Gesa Wilkens, Bo-Hung Liao, Maia L. Werz, Louise Platen, Cho-Chin Cheng, Myriam Tellenbach, Verena Kappler, Viktor Lehner, Hrvoje Mijočević, Catharina Christa, Volker Assfalg, Uwe Heemann, Christoph Schmaderer, Ulrike Protzer, Matthias C. Braunisch, Tanja Bauer, Lutz Renders
Pubblicato in: Frontiers in Immunology, Numero 14, 2023, ISSN 1664-3224
Editore: Frontiers
DOI: 10.3389/fimmu.2023.1172477

Triple-Stranded DNA As a Structural Element in DNA Origami

Autori: Ken Sachenbacher, Ali Khoshouei, Maximilian Nicolas Honemann, Wouter Engelen, Elija Feigl, Hendrik Dietz
Pubblicato in: ACS Nano, Numero 17, 2023, Pagina/e 9014-9024, ISSN 1936-0851
Editore: American Chemical Society
DOI: 10.1021/acsnano.2c11402

Active Nuclear Import of Mammalian Cell-Expressible DNA Origami

Autori: Anna Liedl, Johanna Grießing, Jessica A. Kretzmann, Hendrik Dietz
Pubblicato in: Journal of the American Chemical Society, Numero 145, 2023, Pagina/e 4946-4950, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.2c12733

Exploiting Pan Influenza A and Pan Influenza B Pseudotype Libraries for Efficient Vaccine Antigen Selection

Autori: Del Rosario JMM, da Costa KAS, Asbach B, Ferrara F, Ferrari M, Wells DA, Mann GS, Ameh VO, Sabeta CT, Banyard AC, Kinsley R, Scott SD, Wagner R, Heeney JL, Carnell GW, Temperton NJ.
Pubblicato in: Vaccines, 2021, ISSN 2076-393X
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/vaccines9070741

Gene-encoding DNA origami for mammalian cell expression

Autori: Jessica A. Kretzmann, Anna Liedl, Alba Monferrer, Volodymyr Mykhailiuk, Samuel Beerkens, Hendrik Dietz
Pubblicato in: Nature Communications, Numero 14, 2023, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-023-36601-1

Influenza A (N1-N9) and Influenza B (B/Victoria and B/Yamagata) Neuraminidase Pseudotypes as Tools for Pandemic Preparedness and Improved Influenza Vaccine Design

Autori: da Costa KAS, Del Rosario JMM, Ferrari M, Vishwanath S, Asbach B, Kinsley R, Wagner R, Heeney JL, Carnell GW, Temperton NJ.
Pubblicato in: Vaccines, Numero 2022 Sep 14;10(9), 2022, Pagina/e 1520, ISSN 2076-393X
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/vaccines10091520

Coronavirus Pseudotypes for All Circulating Human Coronaviruses for Quantification of Cross-Neutralizing Antibody Responses

Autori: Sampson AT, Heeney J, Cantoni D, Ferrari M, Sans MS, George C, Di Genova C, Mayora Neto M, Einhauser S, Asbach B, Wagner R, Baxendale H, Temperton N, Carnell G.
Pubblicato in: Viruses (Basel), 2021, ISSN 1999-4915
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v13081579

A serum-stable RNA aptamer specific for SARS-CoV-2 neutralizes viral entry.

Autori: Valero J, Civit L, Dupont DM, Selnihhin D, Reinert LS, Idorn M, Israels BA, Bednarz AM, Bus C, Asbach B, Peterhoff D, Pedersen FS, Birkedal V, Wagner R, Paludan SR, Kjems J.
Pubblicato in: Proc Natl Acad Sci, Numero 2021 Dec 14;118(50), 2021, ISSN 1091-6490
Editore: National Academy of Science
DOI: 10.1073/pnas.2112942118

SARS‐CoV‐2 Productively Infects Human Hepatocytes and Induces Cell Death

Autori: Chunkyu Ko, Cho‐Chin Cheng, Daniele Mistretta, Shubhankar Ambike, Julia Sacherl, Stoyan Velkov, Bo‐Hung Liao, Romina Bester, Merve Gültan, Olga Polezhaeva, Alexander Herrmann, Constanze A. Jakwerth, Carsten B. Schmidt‐Weber, Joachim J. Bugert, Roman Wölfel, Vincent Grass, Sandra Essbauer, Daniel Schnepf, Oliver T. Keppler, Florian W. R. Vondran, Andreas Pichlmair, Carolin Mogler, Gregor Eb
Pubblicato in: Journal of Medical Virology, Numero 97, 2025, ISSN 0146-6615
Editore: John Wiley & Sons Inc.
DOI: 10.1002/jmv.70156

SARS-CoV-2 Neutralization Capacity in Hemodialysis Patients with and without a Fifth Vaccination with the Updated Comirnaty Original/Omicron BA.4-5 Vaccine

Autori: Bo-Hung Liao, Louise Platen, Myriam Grommes, Cho-Chin Cheng, Christopher Holzmann-Littig, Catharina Christa, Bernhard Haller, Verena Kappler, Romina Bester, Maia Lucia Werz, Eva Platen, Peter Eggerer, Laëtitia Tréguer, Claudius Küchle, Christoph Schmaderer, Uwe Heemann, Lutz Renders, Ulrike Protzer, Matthias Christoph Braunisch
Pubblicato in: Vaccines, Numero 12, 2025, Pagina/e 308, ISSN 2076-393X
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/vaccines12030308

Programmable icosahedral shell system for virus trapping

Autori: Christian Sigl, Elena M. Willner, Wouter Engelen, Jessica A. Kretzmann, Ken Sachenbacher, Anna Liedl, Fenna Kolbe, Florian Wilsch, S. Ali Aghvami, Ulrike Protzer, Michael F. Hagan, Seth Fraden, Hendrik Dietz
Pubblicato in: Nature Materials, 2021, ISSN 1476-1122
Editore: Nature Publishing Group
DOI: 10.1038/s41563-021-01020-4

A Multi-Faceted Binding Assessment of Aptamers Targeting the SARS-CoV-2 Spike Protein

Autori: Laia Civit, Nima Moradzadeh, Anna Jonczyk, Patrick Neckermann, Benedikt Asbach, David Peterhoff, Ralf Wagner, Michael Famulok, Günter Mayer, Jørgen Kjems, Julián Valero
Pubblicato in: International Journal of Molecular Sciences, Numero 25, 2025, Pagina/e 4642, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms25094642

Preorganized cyclic modules facilitate the self-assembly of protein nanostructures

Autori: Jaka Snoj, Fabio Lapenta, Roman Jerala
Pubblicato in: Chemical Science, Numero 15, 2024, Pagina/e 3673-3686, ISSN 2041-6520
Editore: Royal Society of Chemistry
DOI: 10.1039/d3sc06658d

Beyond Dimerization: Harnessing Tetrameric Coiled‐Coils for Nanostructure Assembly

Autori: Sara Vidmar, Tamara Šmidlehner, Jana Aupič, Žiga Strmšek, Ajasja Ljubetič, Fei Xiao, Guang Hu, Chuan Liu, Florian Beck, Philipp S. Erdmann, Roman Jerala
Pubblicato in: Angewandte Chemie International Edition, 2024, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202422075

Synthetic virions reveal fatty acid-coupled adaptive immunogenicity of SARS-CoV-2 spike glycoprotein.

Autori: Staufer O, Gupta K, Hernandez Bücher JE, Kohler F, Sigl C, Singh G, Vasileiou K, Yagüe Relimpio A, Macher M, Fabritz S, Dietz H, Cavalcanti Adam EA, Schaffitzel C, Ruggieri A, Platzman I, Berger I, Spatz JP
Pubblicato in: Nat Commun, Numero 2022 Feb 14;13(1), 2022, Pagina/e 868, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-022-28446-x

Coupling of Spectrin Repeat Modules for the Assembly of Nanorods and Presentation of Protein Domains

Autori: Klemen Mezgec, Jaka Snoj, Liza Ulčakar, Ajasja Ljubetič, Magda Tušek Žnidarič, Miha Škarabot, Roman Jerala
Pubblicato in: ACS Nano, Numero 18, 2024, Pagina/e 28748-28763, ISSN 1936-0851
Editore: American Chemical Society
DOI: 10.1021/acsnano.4c07701

The art of designed coiled-coils for the regulation of mammalian cells

Autori: Tjaša Plaper, Erik Rihtar, Taja Železnik Ramuta, Vida Forstnerič, Vid Jazbec, Filip Ivanovski, Mojca Benčina, Roman Jerala
Pubblicato in: Cell Chemical Biology, Numero 31, 2024, Pagina/e 1460-1472, ISSN 2451-9448
Editore: Elsevier
DOI: 10.1016/j.chembiol.2024.06.001

Activation of CD4 T cells during prime immunization determines the success of a therapeutic hepatitis B vaccine in HBV-carrier mouse models

Autori: Jinpeng Su, Livia Brunner, Edanur Ates Oz, Julia Sacherl, Geraldine Frank, Helene Anne Kerth, Frank Thiele, Marian Wiegand, Carolin Mogler, Julio Cesar Aguilar, Percy A. Knolle, Nicolas Collin, Anna D. Kosinska, Ulrike Protzer
Pubblicato in: Journal of Hepatology, Numero 78, 2023, Pagina/e 717-730, ISSN 0168-8278
Editore: Elsevier BV
DOI: 10.1016/j.jhep.2022.12.013

DNA origami traps for large viruses

Autori: Alba Monferrer, Fabian Kohler, Christian Sigl, Michael Schachtner, David Peterhoff, Benedikt Asbach, Ralf Wagner, Hendrik Dietz
Pubblicato in: Cell Reports Physical Science, Numero 4, 2023, Pagina/e 101237, ISSN 2666-3864
Editore: Elsevier
DOI: 10.1016/j.xcrp.2022.101237

A Nanoscaffolded Spike-RBD Vaccine Provides Protection against SARS-CoV-2 with Minimal Anti-Scaffold Response

Autori: Duško Lainšček, Tina Fink, Vida Forstnerič, Iva Hafner-Bratkovič, Sara Orehek, Žiga Strmšek, Mateja Manček-Keber, Peter Pečan, Hana Esih, Špela Malenšek, Jana Aupič, Petra Dekleva, Tjaša Plaper, Sara Vidmar, Lucija Kadunc, Mojca Benčina, Neža Omersa, Gregor Anderluh, Florence Pojer, Kelvin Lau, David Hacker, Bruno E. Correia, David Peterhoff, Ralf Wagner, Valter Bergant, Alexander H
Pubblicato in: Vaccines, Numero 9/5, 2021, Pagina/e 431, ISSN 2076-393X
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/vaccines9050431

Influenza A (N1-N9) and Influenza B (B/Vic and B/Yam) Neuraminidase Pseudotypes as Tools for Pandemic Preparedness and Improved Influenza Vaccine design

Autori: Kelly A.S. da Costa, Joanne Marie M. Del Rosario, Matteo Ferrari, Sneha Vishwanath, Benedikt Asbach, Rebecca Kinsley, Ralf Wagner, Jonathan L. Heeney, George W. Carnell, Nigel J. Temperton
Pubblicato in: Vaccines , 2021, ISSN 2076-393X
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.1101/2021.09.19.460966

Antigen-Triggered Logic-Gating of DNA Nanodevices

Autori: Wouter Engelen; Christian Sigl; Karoline Kadletz; Elena M. Willner; Hendrik Dietz
Pubblicato in: J Am Chem Soc, Numero 1, 2021, Pagina/e 21630-21636, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.1c09967

A Multiplexed Bacterial Two-Hybrid for Rapid Characterization of Protein-Protein Interactions and Iterative Protein Design

Autori: W. Clifford Boldridge, Ajasja Ljubetič, Hwangbeom Kim, Nathan Lubock, Dániel Szilágyi, Jonathan Lee, Andrej Brodnik, Roman Jerala, Sriram Kosuri
Pubblicato in: Nat Commun., 2024, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1101/2020.11.12.377184

Broad-Spectrum Virus Trapping with Heparan Sulfate-Modified DNA Origami Shells

Autori: Alba Monferrer, Jessica A. Kretzmann, Christian Sigl, Pia Sapelza, Anna Liedl, Barbara Wittmann, and Hendrik Dietz
Pubblicato in: ACS Nano, 2022, ISSN 1936-0851
Editore: American Chemical Society
DOI: 10.1021/acsnano.1c11328

Hepatitis B virus neutralization with DNA origami nanoshells

Autori: Elena M. Willner, Fenna Kolbe, Frank Momburg, Ulrike Protzer, Hendrik Dietz
Pubblicato in: ACS Appl Mater Interfaces., 2023, ISSN 1944-8244
Editore: American Chemical Society
DOI: 10.1101/2023.12.20.572526

Mucoadhesive film for oral delivery of vaccines for protection of the respiratory tract

Autori: Hana Esih, Klemen Mezgec, Martina Billmeier, Špela Malenšek, Mojca Benčina, Blaž Grilc, Sara Vidmar, Mirjana Gašperlin, Marjan Bele, Mihaela Zidarn, Tatjana Lejko Zupanc, Tina Morgan, Ingo Jordan, Volker Sandig, Silke Schrödel, Christian Thirion, Ulrike Protzer, Ralf Wagner, Duško Lainšček, Roman Jerala
Pubblicato in: Journal of Controlled Release, Numero 371, 2024, Pagina/e 179-192, ISSN 0168-3659
Editore: Elsevier BV
DOI: 10.1016/j.jconrel.2024.05.041

Applications of HBVenv-specific antibodies as research and therapeutic tools

Autori: Kolbe, Fenna Meret
Pubblicato in: 2023
Editore: Technische Universität München

Programmable DNA origami shell platform for virus neutralization

Autori: Sigl, Christian
Pubblicato in: 2022
Editore: Fakultät für Physik

Functional DNA Origami Shells for Broad-Spectrum Antiviral Applications

Autori: Alba Monferrer i Sureda
Pubblicato in: 2023
Editore: TUM School of Natural Sciences

Nanoschalen aus DNA fangen und neutralisieren Viren

Autori: Christian Sigl, Hendrik Dietz
Pubblicato in: BIOspektrum, Numero 28, 2022, Pagina/e 165-167, ISSN 0947-0867
Editore: Spektrum Akademischer Verlag GmbH
DOI: 10.1007/s12268-022-1729-2

Diritti di proprietà intellettuale

PROGRAMMABLE SHELLS FOR VIRUS ENCAPSULATION

Numero candidatura/pubblicazione: 20 158577
Data: 2020-02-20
Candidato/i: TECHNISCHE UNIVERSITAET MUENCHEN

CONFORMATION SWITCHING NANOSTRUCTURES

Numero candidatura/pubblicazione: 21 169934
Data: 2021-04-22
Candidato/i: TECHNISCHE UNIVERSITAET MUENCHEN

PROGRAMMABLE SHELLS FOR VIRUS ENCAPSULATION

Numero candidatura/pubblicazione: 20 158577
Data: 2020-02-20
Candidato/i: TECHNISCHE UNIVERSITAET MUENCHEN

CONFORMATION SWITCHING NANOSTRUCTURES

Numero candidatura/pubblicazione: 21 169934
Data: 2021-04-22
Candidato/i: TECHNISCHE UNIVERSITAET MUENCHEN

LIPOSOME-RECEPTOR-ASSAY FOR DETECTING NEUTRALIZING ANTIBODIES

Numero candidatura/pubblicazione: 20 217081
Data: 2020-12-23
Candidato/i: UNIVERSITAET REGENSBURG

LIPOSOME-RECEPTOR-ASSAY FOR DETECTING NEUTRALIZING ANTIBODIES

Numero candidatura/pubblicazione: 20 217081
Data: 2020-12-23
Candidato/i: UNIVERSITAET REGENSBURG

Risultati finali

Pubblicazioni

Diritti di proprietà intellettuale

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