Biogenic Organotropic Wetsuits

Informations projet

BOW

N° de convention de subvention: 952183

DOI

10.3030/952183

Projet clôturé

Date de signature de la CE 28 Mai 2020

Date de début 1 Novembre 2020

Date de fin 31 Octobre 2024

Financé au titre de

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coût total

€ 4 442 551,25

Contribution de l’UE

€ 4 442 551,25

Coordonné par

CONSORZIO INTERUNIVERSITARIO PERLO SVILUPPO DEI SISTEMI A GRANDE INTERFASE
Italy

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Livrables

Websites, patent fillings, videos etc. (5)

Promotional material

First version of the promotional material newsletters flyer videos video news release

Training materials elaborated during BOW project
Second promotional material

Second version of the promotional material newsletters flyer videos video news release

Third promotional material

Third version of the promotional material (newsletters, flyer, videos, video news release)

Website, social network profiles and CMS guide

Project web site and social network profiles CMS guide for all partners

Documents, reports (16)

evBOW products in C. elegans

Determination in vivo (C. elegans) of toxicity, stability and biodistribution of evBOW products.

First report on C&D activities

First report on CD activities

Functionalization of EVs

Setting up and validation of strategy to attach different proteinsantibodies or sugars to target cells

SOPs for synthesis of single-core magnetite MBDs

Standard Operating Procedure SOPs on single core magnetite MBDs SPIONS synthesis

Project management handbook

CSGI will produce a project handbook for project management and quality assurance It will define the guidelines for reporting and internal procedures

evBOW products in vitro cellular uptake, cytotoxicity, genotoxicity and mutagenic potential

In vitro evaluation of cellular uptake, cytotoxicity, genotoxicity and mutagenic potential of evBOW products.

evBOW products in M. musculus

Determination in vivo (M.musculus) of toxicity, stability and biodistribution of evBOW products.

evBOW products in vitro and ex vivo immunotoxicity/genotoxicity.

In vitro and ex vivo evaluation of immunotoxicity and genotoxicity of evBOW products.

SOPs for synthesis of multi-core magnetite MBDs

SOPs on multicore magnetite MBDs

Updated report on C&D activities
SOPs for derivatization of multi-core magnetite MBDs

SOPs on derivatized MBDs SPMbeads functionalization

SOPs for derivatization of single-core magnetite MBDs

SOPs on derivatized MBDs SPIONS functionalization

Project Exploitation and Dissemination Plan

First version of the project Exploitation and Dissemination Plan PEDR

Updated Project Exploitation and Dissemination Plan

Updated version of the project Exploitation and Dissemination Plan PEDR

Production of microalgal-derived EVs

We will generate two SOPs related to 1 the cultivation of a microalgal strains suitable for the production of EVs 2 the production and separation of microalgal EVs

Model of evBOW products toxicity

Predictive mathematical model of evBOW products toxicity risk using cell system in vitro

Open Research Data Pilot (4)

Evaluation of evMBDs dynamic parameters using mathematical models

Mathematical model of evMBDs transport in the microfluidic device

Characterization of EVs

A quality check of EDTMSC and microalgal EVs will be generated it will include size distribution density morphology concentration stability in biological fluids and EVaggregate ratio purity

Updated Data Management Plan

An updated Data Management Plan will be elaborated considering the H2020 open access rules and research data management to ensure that outputs are findable, accessible, interoperable and reusable to scientific publications rules

Data Management Plan

A Data Management Plan will be elaborated considering the H2020 open access rules and research data management to ensure that outputs are findable accessible interoperable and reusable to scientific publications rules

Other (1)

Final event

Final event (M48; CSGI) project results will be presented in a final event.

Publications

Peer reviewed articles (34)

Opportunities and Pitfalls of Fluorescent Labeling Methodologies for Extracellular Vesicle Profiling on High-Resolution Single-Particle Platforms

Auteurs: Fortunato D, Mladenović D, Criscuoli M, Loria F, Veiman K-L, Zocco D, Koort K, Zarovni N
Publié dans: International Journal of Molecular Sciences, Numéro 2021, 22(19),, 2021, Page(s) 10510, ISSN 1422-0067
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms221910510

Selective isolation of extracellular vesicles from minimally processed human plasma as a translational strategy for liquid biopsies

Auteurs: Diogo Fortunato; Stavros Giannoukakos; Ana Giménez-Capitán; Michael Hackenberg; Miguel A. Molina-Vila; Nataša Zarovni
Publié dans: Biomarker Research, Numéro Volume 10, Article number: 57 (2022), 2022, ISSN 1741-7007
Éditeur: BioMed Central
DOI: 10.1186/s40364-022-00404-1

Particle profiling of EV-lipoprotein mixtures by AFMnanomechanical imaging

Auteurs: Andrea Ridolfi, Laura Conti, Marco Brucale, Roberto Frigerio, Jacopo Cardellini, Angelo Musicò, Miriam Romano, Andrea Zendrini, Laura Polito, Greta Bergamaschi, Alessandro Gori, Costanza Montis, Stefano Panella, Lucio Barile, Debora Berti, Annalisa Radeghieri, Paolo Bergese, Marina Cretich, Francesco Valle
Publié dans: Journal of Extracellular Vesicles, Numéro Volume 12, Numéro 10, 2023, Page(s) n/a, ISSN 2001-3078
Éditeur: Wiley Periodicals LLC on behalf of International Society for Extracellular Vesicle
DOI: 10.1002/jev2.12349

Membrane Phase Drives the Assembly of Gold Nanoparticles on Biomimetic Lipid Bilayers

Auteurs: Jacopo Cardellini, Lucrezia Caselli, Enrico Lavagna, Sebastian Salassi, Heinz Amenitsch, Martino Calamai, Costanza Montis, Giulia Rossi, and Debora Berti*
Publié dans: The Journal of Physical Chemistry C, Numéro 2022 126 (9), 2022, Page(s) 4483–4494, ISSN 1932-7447
Éditeur: American Chemical Society
DOI: 10.1021/acs.jpcc.1c08914

Fluorescent Single-Core and Multi-Core Nanoprobes as Cell Trackers and Magnetic Nanoheaters

Auteurs: García Acevedo, Pelayo; González Gómez Manuel Antonio; Arnosa Prieto Ángela; de Castro Alves Lisandra; Seco Gudiña Román; Piñeiro Yolanda; Rivas José
Publié dans: Magnetochemistry, Numéro Volume 8; Numéro 8, 2022, Page(s) 83, ISSN 2312-7481
Éditeur: MDPI
DOI: 10.3390/magnetochemistry8080083

Nanoscale structural and mechanical characterization of thin bicontinuous cubic phase lipid films

Auteurs: Andrea Ridolfi, Ben Humphreys, Lucrezia Caselli, Costanza Montis, Tommy Nylander, Debora Berti, Marco Brucale, Francesco Valle
Publié dans: Colloids and Surfaces B: Biointerfaces, Numéro Volume 210, February 2022, 2022, Page(s) 112231, ISSN 0927-7765
Éditeur: Elsevier BV
DOI: 10.1016/j.colsurfb.2021.112231

Interaction of Metallic Nanoparticles With Biomimetic Lipid Liquid Crystalline Cubic Interfaces

Auteurs: Jacopo Cardellini, Costanza Montis, Francesco Barbero, Ilaria De Santis, Lucrezia Caselli and Debora Berti
Publié dans: Frontiers in bioengineering and biotechnology - Sec. Nanobiotechnology, Numéro Volume 10 - 15 March 2022, 2022, ISSN 2296-4185
Éditeur: Frontiers
DOI: 10.3389/fbioe.2022.848687

Significant Surface Spin Effects and Exchange Bias in Iron Oxide-Based Hollow Magnetic Nanoparticles

Auteurs: Pelayo García Acevedo; Manuel A. González Gómez; Ángela Arnosa Prieto; Jose S. Garitaonandia; Yolanda Piñeiro; José Rivas
Publié dans: Nanomaterials, Numéro Nanomaterials 2022, 12(3), 2022, Page(s) 456, ISSN 2079-4991
Éditeur: Nanomaterials
DOI: 10.3390/nano12030456

Two Dimensional-Difference in Gel Electrophoresis (2D-DIGE) Proteomic Approach for the Identification of Biomarkers in Endometrial Cancer Serum

Auteurs: Blendi Ura, Stefania Biffi, Lorenzo Monasta, Giorgio Arrigoni, Ilaria Battisti, Giovanni Di Lorenzo, Federico Romano, Michelangelo Aloisio, Fulvio Celsi, Riccardo Addobbati, Francesco Valle, Enrico Rampazzo, Marco Brucale, Andrea Ridolfi, Danilo Licastro, Giuseppe Ricci
Publié dans: Cancers, Numéro 13/14, 2021, Page(s) 3639, ISSN 2072-6694
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cancers13143639

Versatile magnetic confguration for the control and manipulation of superparamagnetic nanoparticles

Auteurs: Surpi Alessandro; Shelyakova Tatiana; Murgia Mauro; Rivas José; Piñeiro Yolanda; Greco Pierpaolo; Fini Milena; Dediu Valentin Alek
Publié dans: Scientific Reports, Numéro 13, Article number: 5301 (2023), 2023, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-023-32299-9

Magnetic separation and concentration of Aβ 1–42 molecules dispersed at the threshold concentration for Alzheimer’s disease diagnosis in clinically-relevant volumes of sample

Auteurs: Alessandro Surpi, Mauro Murgia, Sonia López-Amoedo, Manuel A. González-Gómez, Yolanda Piñeiro, José Rivas, Valeria Perugini, Matteo Santin, Tomás Sobrino, Pierpaolo Greco, Francisco Campos, Valentin Alek Dediu
Publié dans: Journal of Nanobiotechnology, Numéro 21, Article number: 329 (2023), 2023, ISSN 1477-3155
Éditeur: BioMed Central
DOI: 10.1186/s12951-023-02095-8

Biocompatible magnetic gelatin nanoparticles with enhanced MRI contrast performance prepared by single-step desolvation method

Auteurs: C Teijeiro-Valiño, M A González Gómez, S Yáñez, P García Acevedo, A Arnosa Prieto, S Belderbos, W Gsell, U Himmelreich, Y Piñeiro, J Rivas
Publié dans: Nano Express, Numéro 2/2, 2021, Page(s) 020011, ISSN 2632-959X
Éditeur: https://ioppublishing.org/
DOI: 10.1088/2632-959x/abf58e

Impact of the flame retardant 2,2’4,4’-tetrabromodiphenyl ether (PBDE-47) in THP-1 macrophage-like cell function via small extracellular vesicles

Auteurs: Valeria Longo, Noemi Aloi, Alessandra Longo, Giorgia Adamo, Antonella Bongiovanni and Paolo Colombo
Publié dans: Front. Immunol., 06 January 2023 Sec. Molecular Innate Immunity, Numéro Volume 13 - 2022, 2023, ISSN 1664-3224
Éditeur: Frontiers
DOI: 10.3389/fimmu.2022.1069207

Role of Dipolar Interactions on the Determination of the Effective Magnetic Anisotropy in Iron Oxide Nanoparticles

Auteurs: Pelayo García-Acevedo, Manuel A. González-Gómez, Ángela Arnosa-Prieto, Lisandra de Castro-Alves, Yolanda Piñeiro, and José Rivas
Publié dans: Advanced Sciences, Numéro Volume 10, Numéro 5, February 14, 2023, 2023, Page(s) 2203397, ISSN 2198-3844
Éditeur: Wiley-VCH GmbH
DOI: 10.1002/advs.202203397

Multiparametric Orthogonal Characterization of Extracellular Vesicles by Liquid Chromatography Combined with In-Line Light Scattering and Fluorescence Detection

Auteurs: Karl Normak, Marcell Papp, Michael Ullmann, Carolina Paganini, Mauro Manno, Antonella Bongiovanni, Paolo Bergese and Paolo Arosio
Publié dans: Analytical Chemistry, Numéro Volume 95, Numéro 33, August 22, 2023, 2023, Page(s) 12195-12574, ISSN 0003-2700
Éditeur: American Chemical Society
DOI: 10.1021/acs.analchem.3c02108

High-Yield Separation of Extracellular Vesicles Using Programmable Zwitterionic Coacervates

Auteurs: Carolina Paganini, Umberto Capasso Palmiero,* Sabrina Picciotto, Alessandro Molinelli, Ilaria Porello, Giorgia Adamo, Mauro Manno, Antonella Bongiovanni, and Paolo Arosio
Publié dans: Small, Numéro 16136810, 2022, ISSN 1613-6810
Éditeur: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.202204736

Comparative effects of extracellular vesicles and liposomal nanocarriers on bleomycin-induced stress in A549 human adenocarcinoma cells

Auteurs: Thomas Conlon, Maximilian Schaaf, Ana Mateos-Maroto, Sabrina Picciotto, Svenja Morsbach, Giorgia Adamo, Shutian Si, Ingo Lieberwirth, Christine Rosenauer, Katharina Landfester, Antonella Bongiovanni, Nicolas Touzet
Publié dans: Biomedicine & Pharmacotherapy, Numéro 187, 2025, Page(s) 118081, ISSN 0753-3322
Éditeur: Elsevier Masson
DOI: 10.1016/j.biopha.2025.118081

Synthesis and Characterization of a Biocompatible Nanoplatform Based on Silica-Embedded SPIONs Functionalized with Polydopamine

Auteurs: Miriam Romano, Manuel Antonio González Gómez, Pamela Santonicola, Noemi Aloi, Svenja Offer, Jana Pantzke, Samuele Raccosta, Valeria Longo, Alessandro Surpi, Silvia Alacqua, Giuseppina Zampi, Valentin Alek Dediu, Bernhard Michalke, Ralf Zimmerman, Mauro Manno, Yolanda Piñeiro, Paolo Colombo, Elia Di Schiavi, José Rivas, Paolo Bergese, and Sebastiano Di Bucchianico
Publié dans: ACS Biomaterials Science & Engineering, Numéro 2023, 9, 1, 2023, Page(s) 303–317, ISSN 2373-9878
Éditeur: American Chemical Society
DOI: 10.1021/acsbiomaterials.2c00946

Extracellular Vesicles From Microalgae: Uptake Studies in Human Cells and Caenorhabditis elegans

Auteurs: Picciotto; Santonicola; Paterna; Rao; Raccosta; Romancino; Noto; Touzet; Manno; Di Schiavi; Bongiovanni; Adamo
Publié dans: Frontiers in bioengineering and biotechnology - Sec. Nanobiotechnology, Numéro Volume 10 - 24 March 2022, 2022, ISSN 2296-4185
Éditeur: Frontiers
DOI: 10.3389/fbioe.2022.830189

An alternative in vitro model considering cell-cell interactions in fiber-induced pulmonary fibrosis

Auteurs: Jana Pantzke and Svenja Offer and Elias J. Zimmermann and Evelyn Kuhn and Thorsten Streibel and Sebastian Oeder and Sebastiano Di Bucchianico and Ralf Zimmermann
Publié dans: Toxicology Mechanisms and Methods, 2022, ISSN 1537-6516
Éditeur: Taylor & Francis
DOI: 10.1080/15376516.2022.2156008

Fluorescent Magnetic Mesoporous Nanoprobes for Biotechnological Enhancement Procedures in Gene Therapy

Auteurs: González-Gómez MA, Seco-Gudiña R, García-Acevedo P, Arnosa-Prieto Á, de Castro-Alves L, Piñeiro Y, Rivas J.
Publié dans: Magnetochemistry, Numéro Magnetochemistry 2023, 9(3), 2023, Page(s) 67, ISSN 2312-7481
Éditeur: Magnetochemistry
DOI: 10.3390/magnetochemistry9030067

Probing the coverage of nanoparticles by biomimetic membranes through nanoplasmonics

Auteurs: Cardellini, Jacopo; Ridolfi, Andrea; Donati, Melissa; Giampietro, Valentina; Severi, Mirko; Brucale, Marco; Valle, Francesco; Bergese, Paolo; Montis, Costanza; Caselli, Lucrezia; Berti, Debora
Publié dans: Journal of Colloid and Interface Science, Numéro Volume 640, 15 June 2023, 2023, Page(s) Pages 100-109, ISSN 0021-9797
Éditeur: Academic Press
DOI: 10.1016/j.jcis.2023.02.073

Malignant‐ascites‐derived small extracellular vesicles in advanced ovarian cancer patients: insights into the dynamics of the extracellular matrix

Auteurs: Barbara Bortot, Maura Apollonio, Enrico Rampazzo, Francesco Valle, Marco Brucale, Andrea Ridolfi, Blendi Ura, Riccardo Addobbati, Giovanni Di Lorenzo, Federico Romano, Francesca Buonomo, Chiara Ripepi, Giuseppe Ricci, Stefania Biffi
Publié dans: Molecular Oncology, Numéro Volume15, Numéro12 December 2021, 2021, Page(s) Pages 3596-3614, ISSN 1574-7891
Éditeur: Elsevier BV
DOI: 10.1002/1878-0261.13110

Large‐scale production of extracellular vesicles: Report on the “massivEVs” ISEV workshop

Auteurs: Lucia Paolini; Marta Monguió‐Tortajada; Marta Costa; Fabio Antenucci; Mario Barilani; Marta Clos‐Sansalvador; André Cronemberger Andrade; Tom A. P. Driedonks; Sara Giancaterino; Stephanie M. Kronstadt; Rachel R. Mizenko; Muhammad Nawaz; Xabier Osteikoetxea; Carla Pereira; Surya Shrivastava; Anders Toftegaard Boysen; Simonides Immanuel van de Wakker; Martijn J. C. van Herwijnen; Xiaoqin Wang;
Publié dans: Paolini , L , Monguió-Tortajada , M , Costa , M , Antenucci , F , Barilani , M , Clos-Sansalvador , M , Andrade , A C , Driedonks , T A P , Giancaterino , S , Kronstadt , S M , Mizenko , R R , Nawaz , M , Osteikoetxea , X , Pereira , C , Shrivastava , S , Boysen , A T , van de Wakker , S I , van Herwijnen , M J C , Wang , X , Watson , D C , Gimona , M , Kaparakis-Liaskos , M , Konstantinov , K ,, Numéro 16, 2022, ISSN 2768-2811
Éditeur: Journal of Extracellular Biology
DOI: 10.1002/jex2.63

Bioactivity of autotrophic and mixotrophic Tetraselmis chuii (CCAP 66/21B) extracts against H2O2-mediated stress in A549 cells.

Auteurs: Thomas Conlon, Helen Herbert, Eva Campion, Nicolas Touzet
Publié dans: Process Biochemistry, Numéro 155, 2025, Page(s) 117-127, ISSN 1359-5113
Éditeur: Elsevier Applied Science
DOI: 10.1016/j.procbio.2025.05.007

Effect of glycerol and H3PO4 on the bioactivity and degradability of rod-like SBA-15 particles with active surface for bone tissue engineering applications

Auteurs: Zulema Vargas-Osorio, Artem Klotschan, Marcela Arango-Ospina, Yolanda Piñeiro, Liliana Liverani, José Rivas, Martin Michálek, Dušan Galusek, Aldo R.Boccaccini
Publié dans: Microporous and Mesoporous Materials, Numéro Volume 329, January 2022, 2021, Page(s) 111543, ISSN 1387-1811
Éditeur: Elsevier BV
DOI: 10.1016/j.micromeso.2021.111543

Isolation of Extracellular Vesicles From Microalgae: A Renewable and Scalable Bioprocess

Auteurs: Angela Paterna; Estella Rao; Giorgia Adamo; Samuele Raccosta; Sabrina Picciotto; Daniele Romancino; Rosina Noto; Nicolas Touzet; Antonella Bongiovanni; Mauro Manno
Publié dans: Frontiers in bioengineering and biotechnology - Sec. Nanobiotechnology, Numéro Volume 10 - 14 March 2022, 2022, ISSN 2296-4185
Éditeur: Frontiers
DOI: 10.3389/fbioe.2022.836747

On the interaction and nanoplasmonics of gold nanoparticles and lipoproteins

Auteurs: Andrea Zendrini, Jacopo Cardellini, Roberto Frigerio, Marianna Bertoni, Debora Berti, Paolo Bergese
Publié dans: JCIS Open, Special issue for 11th Colloids Conference, Numéro Volume 11, October 2023, Article 100088, 2023, ISSN 2666-934X
Éditeur: Elsevier
DOI: 10.1016/j.jciso.2023.100088

The biomolecular corona of extracellular nanoparticles holds new promises for advancing clinical molecular diagnostics

Auteurs: Annalisa Radeghieri and Paolo Bergese
Publié dans: Expert Review of Molecular Diagnostics, 2023, ISSN 1744-8352
Éditeur: Taylor & Francis
DOI: 10.1080/14737159.2023.2215927

Extracellular Vesicles Analysis in the COVID-19 Era: Insights on Serum Inactivation Protocols towards Downstream Isolation and Analysis

Auteurs: Roberto Frigerio, Angelo Musicò, Marco Brucale, Andrea Ridolfi, Silvia Galbiati, Riccardo Vago, Greta Bergamaschi, Anna Maria Ferretti, Marcella Chiari, Francesco Valle, Alessandro Gori, Marina Cretich
Publié dans: Cells, Numéro 10/3, 2021, Page(s) 544, ISSN 2073-4409
Éditeur: Cells
DOI: 10.3390/cells10030544

Stiffness of Fluid and Gel Phase Lipid Nanovesicles: Weighting the Contributions of Membrane Bending Modulus and Luminal Pressurization

Auteurs: Andrea Ridolfi, Lucrezia Caselli, Matteo Baldoni, Costanza Montis, Francesco Mercuri, Debora Berti, Francesco Valle, Marco Brucale
Publié dans: Langmuir, 2021, ISSN 0743-7463
Éditeur: American Chemical Society
DOI: 10.1021/acs.langmuir.1c01660

Surface functionalization of extracellular vesicle nanoparticles with antibodies: a first study on the protein corona “variable”

Auteurs: Angelo Musicò, Rossella Zenatelli, Miriam Romano, Andrea Zendrini, Silvia Alacqua, Selene Tassoni, Lucia Paolini, Chiara Urbinati, Marco Rusnati, Paolo Bergese, Giuseppe Pomarico, and Annalisa Radeghieri
Publié dans: Nanoscale Advances, 5, Numéro Numéro 18, 2023, 2023, Page(s) 4703-4717, ISSN 2516-0230
Éditeur: ROYAL SOCIETY OF CHEMISTRY
DOI: 10.1039/d3na00280b

A plasmon-based nanoruler to probe the mechanical properties of synthetic and biogenic nanosized lipid vesicles

Auteurs: Lucrezia Caselli, Andrea Ridolfi, Jacopo Cardellini, Lewis Sharpnack, Lucia Paolini, Marco Brucale, Francesco Valle, Costanza Montis, Paolo Bergese, Debora Berti
Publié dans: Nanoscale Horizons, Numéro 6/7, 2021, Page(s) 543-550, ISSN 2055-6756
Éditeur: Nanoscale Horizons
DOI: 10.1039/d1nh00012h

Extracellular vesicles from the microalga Tetraselmis chuii are biocompatible and exhibit unique bone tropism along with antioxidant and anti-inflammatory properties

Auteurs: Giorgia Adamo, Pamela Santonicola, Sabrina Picciotto, Paola Gargano, Aldo Nicosia, Valeria Longo, Noemi Aloi, Daniele P. Romancino, Angela Paterna, Estella Rao, Samuele Raccosta, Rosina Noto, Monica Salamone, Irene Deidda, Salvatore Costa, Caterina Di Sano, Giuseppina Zampi, Svenja Morsbach, Katharina Landfester, Paolo Colombo, Mingxing Wei, Paolo Bergese, Nicolas Touzet, Mauro Manno, Elia Di Schi
Publié dans: Communications Biology, Numéro 7, 2024, ISSN 2399-3642
Éditeur: Communications Biology (Nature)
DOI: 10.1038/s42003-024-06612-9