Biogenic Organotropic Wetsuits

Información del proyecto

BOW

Identificador del acuerdo de subvención: 952183

DOI

10.3030/952183

Proyecto cerrado

Fecha de la firma de la CE 28 Mayo 2020

Fecha de inicio 1 Noviembre 2020

Fecha de finalización 31 Octubre 2024

Financiado con arreglo a

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coste total

€ 4 442 551,25

Aportación de la UE

€ 4 442 551,25

Coordinado por

CONSORZIO INTERUNIVERSITARIO PERLO SVILUPPO DEI SISTEMI A GRANDE INTERFASE
Italy

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Websites, patent fillings, videos etc. (5)

Promotional material

First version of the promotional material newsletters flyer videos video news release

Training materials elaborated during BOW project
Second promotional material

Second version of the promotional material newsletters flyer videos video news release

Third promotional material

Third version of the promotional material (newsletters, flyer, videos, video news release)

Website, social network profiles and CMS guide

Project web site and social network profiles CMS guide for all partners

Documents, reports (16)

evBOW products in C. elegans

Determination in vivo (C. elegans) of toxicity, stability and biodistribution of evBOW products.

First report on C&D activities

First report on CD activities

Functionalization of EVs

Setting up and validation of strategy to attach different proteinsantibodies or sugars to target cells

SOPs for synthesis of single-core magnetite MBDs

Standard Operating Procedure SOPs on single core magnetite MBDs SPIONS synthesis

Project management handbook

CSGI will produce a project handbook for project management and quality assurance It will define the guidelines for reporting and internal procedures

evBOW products in vitro cellular uptake, cytotoxicity, genotoxicity and mutagenic potential

In vitro evaluation of cellular uptake, cytotoxicity, genotoxicity and mutagenic potential of evBOW products.

evBOW products in M. musculus

Determination in vivo (M.musculus) of toxicity, stability and biodistribution of evBOW products.

evBOW products in vitro and ex vivo immunotoxicity/genotoxicity.

In vitro and ex vivo evaluation of immunotoxicity and genotoxicity of evBOW products.

SOPs for synthesis of multi-core magnetite MBDs

SOPs on multicore magnetite MBDs

Updated report on C&D activities
SOPs for derivatization of multi-core magnetite MBDs

SOPs on derivatized MBDs SPMbeads functionalization

SOPs for derivatization of single-core magnetite MBDs

SOPs on derivatized MBDs SPIONS functionalization

Project Exploitation and Dissemination Plan

First version of the project Exploitation and Dissemination Plan PEDR

Updated Project Exploitation and Dissemination Plan

Updated version of the project Exploitation and Dissemination Plan PEDR

Production of microalgal-derived EVs

We will generate two SOPs related to 1 the cultivation of a microalgal strains suitable for the production of EVs 2 the production and separation of microalgal EVs

Model of evBOW products toxicity

Predictive mathematical model of evBOW products toxicity risk using cell system in vitro

Open Research Data Pilot (4)

Evaluation of evMBDs dynamic parameters using mathematical models

Mathematical model of evMBDs transport in the microfluidic device

Characterization of EVs

A quality check of EDTMSC and microalgal EVs will be generated it will include size distribution density morphology concentration stability in biological fluids and EVaggregate ratio purity

Updated Data Management Plan

An updated Data Management Plan will be elaborated considering the H2020 open access rules and research data management to ensure that outputs are findable, accessible, interoperable and reusable to scientific publications rules

Data Management Plan

A Data Management Plan will be elaborated considering the H2020 open access rules and research data management to ensure that outputs are findable accessible interoperable and reusable to scientific publications rules

Other (1)

Final event

Final event (M48; CSGI) project results will be presented in a final event.

Publicaciones

Peer reviewed articles (34)

Opportunities and Pitfalls of Fluorescent Labeling Methodologies for Extracellular Vesicle Profiling on High-Resolution Single-Particle Platforms

Autores: Fortunato D, Mladenović D, Criscuoli M, Loria F, Veiman K-L, Zocco D, Koort K, Zarovni N
Publicado en: International Journal of Molecular Sciences, Edición 2021, 22(19),, 2021, Página(s) 10510, ISSN 1422-0067
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms221910510

Selective isolation of extracellular vesicles from minimally processed human plasma as a translational strategy for liquid biopsies

Autores: Diogo Fortunato; Stavros Giannoukakos; Ana Giménez-Capitán; Michael Hackenberg; Miguel A. Molina-Vila; Nataša Zarovni
Publicado en: Biomarker Research, Edición Volume 10, Article number: 57 (2022), 2022, ISSN 1741-7007
Editor: BioMed Central
DOI: 10.1186/s40364-022-00404-1

Particle profiling of EV-lipoprotein mixtures by AFMnanomechanical imaging

Autores: Andrea Ridolfi, Laura Conti, Marco Brucale, Roberto Frigerio, Jacopo Cardellini, Angelo Musicò, Miriam Romano, Andrea Zendrini, Laura Polito, Greta Bergamaschi, Alessandro Gori, Costanza Montis, Stefano Panella, Lucio Barile, Debora Berti, Annalisa Radeghieri, Paolo Bergese, Marina Cretich, Francesco Valle
Publicado en: Journal of Extracellular Vesicles, Edición Volume 12, Edición 10, 2023, Página(s) n/a, ISSN 2001-3078
Editor: Wiley Periodicals LLC on behalf of International Society for Extracellular Vesicle
DOI: 10.1002/jev2.12349

Membrane Phase Drives the Assembly of Gold Nanoparticles on Biomimetic Lipid Bilayers

Autores: Jacopo Cardellini, Lucrezia Caselli, Enrico Lavagna, Sebastian Salassi, Heinz Amenitsch, Martino Calamai, Costanza Montis, Giulia Rossi, and Debora Berti*
Publicado en: The Journal of Physical Chemistry C, Edición 2022 126 (9), 2022, Página(s) 4483–4494, ISSN 1932-7447
Editor: American Chemical Society
DOI: 10.1021/acs.jpcc.1c08914

Fluorescent Single-Core and Multi-Core Nanoprobes as Cell Trackers and Magnetic Nanoheaters

Autores: García Acevedo, Pelayo; González Gómez Manuel Antonio; Arnosa Prieto Ángela; de Castro Alves Lisandra; Seco Gudiña Román; Piñeiro Yolanda; Rivas José
Publicado en: Magnetochemistry, Edición Volume 8; Edición 8, 2022, Página(s) 83, ISSN 2312-7481
Editor: MDPI
DOI: 10.3390/magnetochemistry8080083

Nanoscale structural and mechanical characterization of thin bicontinuous cubic phase lipid films

Autores: Andrea Ridolfi, Ben Humphreys, Lucrezia Caselli, Costanza Montis, Tommy Nylander, Debora Berti, Marco Brucale, Francesco Valle
Publicado en: Colloids and Surfaces B: Biointerfaces, Edición Volume 210, February 2022, 2022, Página(s) 112231, ISSN 0927-7765
Editor: Elsevier BV
DOI: 10.1016/j.colsurfb.2021.112231

Interaction of Metallic Nanoparticles With Biomimetic Lipid Liquid Crystalline Cubic Interfaces

Autores: Jacopo Cardellini, Costanza Montis, Francesco Barbero, Ilaria De Santis, Lucrezia Caselli and Debora Berti
Publicado en: Frontiers in bioengineering and biotechnology - Sec. Nanobiotechnology, Edición Volume 10 - 15 March 2022, 2022, ISSN 2296-4185
Editor: Frontiers
DOI: 10.3389/fbioe.2022.848687

Significant Surface Spin Effects and Exchange Bias in Iron Oxide-Based Hollow Magnetic Nanoparticles

Autores: Pelayo García Acevedo; Manuel A. González Gómez; Ángela Arnosa Prieto; Jose S. Garitaonandia; Yolanda Piñeiro; José Rivas
Publicado en: Nanomaterials, Edición Nanomaterials 2022, 12(3), 2022, Página(s) 456, ISSN 2079-4991
Editor: Nanomaterials
DOI: 10.3390/nano12030456

Two Dimensional-Difference in Gel Electrophoresis (2D-DIGE) Proteomic Approach for the Identification of Biomarkers in Endometrial Cancer Serum

Autores: Blendi Ura, Stefania Biffi, Lorenzo Monasta, Giorgio Arrigoni, Ilaria Battisti, Giovanni Di Lorenzo, Federico Romano, Michelangelo Aloisio, Fulvio Celsi, Riccardo Addobbati, Francesco Valle, Enrico Rampazzo, Marco Brucale, Andrea Ridolfi, Danilo Licastro, Giuseppe Ricci
Publicado en: Cancers, Edición 13/14, 2021, Página(s) 3639, ISSN 2072-6694
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cancers13143639

Versatile magnetic confguration for the control and manipulation of superparamagnetic nanoparticles

Autores: Surpi Alessandro; Shelyakova Tatiana; Murgia Mauro; Rivas José; Piñeiro Yolanda; Greco Pierpaolo; Fini Milena; Dediu Valentin Alek
Publicado en: Scientific Reports, Edición 13, Article number: 5301 (2023), 2023, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-023-32299-9

Magnetic separation and concentration of Aβ 1–42 molecules dispersed at the threshold concentration for Alzheimer’s disease diagnosis in clinically-relevant volumes of sample

Autores: Alessandro Surpi, Mauro Murgia, Sonia López-Amoedo, Manuel A. González-Gómez, Yolanda Piñeiro, José Rivas, Valeria Perugini, Matteo Santin, Tomás Sobrino, Pierpaolo Greco, Francisco Campos, Valentin Alek Dediu
Publicado en: Journal of Nanobiotechnology, Edición 21, Article number: 329 (2023), 2023, ISSN 1477-3155
Editor: BioMed Central
DOI: 10.1186/s12951-023-02095-8

Biocompatible magnetic gelatin nanoparticles with enhanced MRI contrast performance prepared by single-step desolvation method

Autores: C Teijeiro-Valiño, M A González Gómez, S Yáñez, P García Acevedo, A Arnosa Prieto, S Belderbos, W Gsell, U Himmelreich, Y Piñeiro, J Rivas
Publicado en: Nano Express, Edición 2/2, 2021, Página(s) 020011, ISSN 2632-959X
Editor: https://ioppublishing.org/
DOI: 10.1088/2632-959x/abf58e

Impact of the flame retardant 2,2’4,4’-tetrabromodiphenyl ether (PBDE-47) in THP-1 macrophage-like cell function via small extracellular vesicles

Autores: Valeria Longo, Noemi Aloi, Alessandra Longo, Giorgia Adamo, Antonella Bongiovanni and Paolo Colombo
Publicado en: Front. Immunol., 06 January 2023 Sec. Molecular Innate Immunity, Edición Volume 13 - 2022, 2023, ISSN 1664-3224
Editor: Frontiers
DOI: 10.3389/fimmu.2022.1069207

Role of Dipolar Interactions on the Determination of the Effective Magnetic Anisotropy in Iron Oxide Nanoparticles

Autores: Pelayo García-Acevedo, Manuel A. González-Gómez, Ángela Arnosa-Prieto, Lisandra de Castro-Alves, Yolanda Piñeiro, and José Rivas
Publicado en: Advanced Sciences, Edición Volume 10, Edición 5, February 14, 2023, 2023, Página(s) 2203397, ISSN 2198-3844
Editor: Wiley-VCH GmbH
DOI: 10.1002/advs.202203397

Multiparametric Orthogonal Characterization of Extracellular Vesicles by Liquid Chromatography Combined with In-Line Light Scattering and Fluorescence Detection

Autores: Karl Normak, Marcell Papp, Michael Ullmann, Carolina Paganini, Mauro Manno, Antonella Bongiovanni, Paolo Bergese and Paolo Arosio
Publicado en: Analytical Chemistry, Edición Volume 95, Edición 33, August 22, 2023, 2023, Página(s) 12195-12574, ISSN 0003-2700
Editor: American Chemical Society
DOI: 10.1021/acs.analchem.3c02108

High-Yield Separation of Extracellular Vesicles Using Programmable Zwitterionic Coacervates

Autores: Carolina Paganini, Umberto Capasso Palmiero,* Sabrina Picciotto, Alessandro Molinelli, Ilaria Porello, Giorgia Adamo, Mauro Manno, Antonella Bongiovanni, and Paolo Arosio
Publicado en: Small, Edición 16136810, 2022, ISSN 1613-6810
Editor: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.202204736

Comparative effects of extracellular vesicles and liposomal nanocarriers on bleomycin-induced stress in A549 human adenocarcinoma cells

Autores: Thomas Conlon, Maximilian Schaaf, Ana Mateos-Maroto, Sabrina Picciotto, Svenja Morsbach, Giorgia Adamo, Shutian Si, Ingo Lieberwirth, Christine Rosenauer, Katharina Landfester, Antonella Bongiovanni, Nicolas Touzet
Publicado en: Biomedicine & Pharmacotherapy, Edición 187, 2025, Página(s) 118081, ISSN 0753-3322
Editor: Elsevier Masson
DOI: 10.1016/j.biopha.2025.118081

Synthesis and Characterization of a Biocompatible Nanoplatform Based on Silica-Embedded SPIONs Functionalized with Polydopamine

Autores: Miriam Romano, Manuel Antonio González Gómez, Pamela Santonicola, Noemi Aloi, Svenja Offer, Jana Pantzke, Samuele Raccosta, Valeria Longo, Alessandro Surpi, Silvia Alacqua, Giuseppina Zampi, Valentin Alek Dediu, Bernhard Michalke, Ralf Zimmerman, Mauro Manno, Yolanda Piñeiro, Paolo Colombo, Elia Di Schiavi, José Rivas, Paolo Bergese, and Sebastiano Di Bucchianico
Publicado en: ACS Biomaterials Science & Engineering, Edición 2023, 9, 1, 2023, Página(s) 303–317, ISSN 2373-9878
Editor: American Chemical Society
DOI: 10.1021/acsbiomaterials.2c00946

Extracellular Vesicles From Microalgae: Uptake Studies in Human Cells and Caenorhabditis elegans

Autores: Picciotto; Santonicola; Paterna; Rao; Raccosta; Romancino; Noto; Touzet; Manno; Di Schiavi; Bongiovanni; Adamo
Publicado en: Frontiers in bioengineering and biotechnology - Sec. Nanobiotechnology, Edición Volume 10 - 24 March 2022, 2022, ISSN 2296-4185
Editor: Frontiers
DOI: 10.3389/fbioe.2022.830189

An alternative in vitro model considering cell-cell interactions in fiber-induced pulmonary fibrosis

Autores: Jana Pantzke and Svenja Offer and Elias J. Zimmermann and Evelyn Kuhn and Thorsten Streibel and Sebastian Oeder and Sebastiano Di Bucchianico and Ralf Zimmermann
Publicado en: Toxicology Mechanisms and Methods, 2022, ISSN 1537-6516
Editor: Taylor & Francis
DOI: 10.1080/15376516.2022.2156008

Fluorescent Magnetic Mesoporous Nanoprobes for Biotechnological Enhancement Procedures in Gene Therapy

Autores: González-Gómez MA, Seco-Gudiña R, García-Acevedo P, Arnosa-Prieto Á, de Castro-Alves L, Piñeiro Y, Rivas J.
Publicado en: Magnetochemistry, Edición Magnetochemistry 2023, 9(3), 2023, Página(s) 67, ISSN 2312-7481
Editor: Magnetochemistry
DOI: 10.3390/magnetochemistry9030067

Probing the coverage of nanoparticles by biomimetic membranes through nanoplasmonics

Autores: Cardellini, Jacopo; Ridolfi, Andrea; Donati, Melissa; Giampietro, Valentina; Severi, Mirko; Brucale, Marco; Valle, Francesco; Bergese, Paolo; Montis, Costanza; Caselli, Lucrezia; Berti, Debora
Publicado en: Journal of Colloid and Interface Science, Edición Volume 640, 15 June 2023, 2023, Página(s) Pages 100-109, ISSN 0021-9797
Editor: Academic Press
DOI: 10.1016/j.jcis.2023.02.073

Malignant‐ascites‐derived small extracellular vesicles in advanced ovarian cancer patients: insights into the dynamics of the extracellular matrix

Autores: Barbara Bortot, Maura Apollonio, Enrico Rampazzo, Francesco Valle, Marco Brucale, Andrea Ridolfi, Blendi Ura, Riccardo Addobbati, Giovanni Di Lorenzo, Federico Romano, Francesca Buonomo, Chiara Ripepi, Giuseppe Ricci, Stefania Biffi
Publicado en: Molecular Oncology, Edición Volume15, Edición12 December 2021, 2021, Página(s) Pages 3596-3614, ISSN 1574-7891
Editor: Elsevier BV
DOI: 10.1002/1878-0261.13110

Large‐scale production of extracellular vesicles: Report on the “massivEVs” ISEV workshop

Autores: Lucia Paolini; Marta Monguió‐Tortajada; Marta Costa; Fabio Antenucci; Mario Barilani; Marta Clos‐Sansalvador; André Cronemberger Andrade; Tom A. P. Driedonks; Sara Giancaterino; Stephanie M. Kronstadt; Rachel R. Mizenko; Muhammad Nawaz; Xabier Osteikoetxea; Carla Pereira; Surya Shrivastava; Anders Toftegaard Boysen; Simonides Immanuel van de Wakker; Martijn J. C. van Herwijnen; Xiaoqin Wang;
Publicado en: Paolini , L , Monguió-Tortajada , M , Costa , M , Antenucci , F , Barilani , M , Clos-Sansalvador , M , Andrade , A C , Driedonks , T A P , Giancaterino , S , Kronstadt , S M , Mizenko , R R , Nawaz , M , Osteikoetxea , X , Pereira , C , Shrivastava , S , Boysen , A T , van de Wakker , S I , van Herwijnen , M J C , Wang , X , Watson , D C , Gimona , M , Kaparakis-Liaskos , M , Konstantinov , K ,, Edición 16, 2022, ISSN 2768-2811
Editor: Journal of Extracellular Biology
DOI: 10.1002/jex2.63

Bioactivity of autotrophic and mixotrophic Tetraselmis chuii (CCAP 66/21B) extracts against H2O2-mediated stress in A549 cells.

Autores: Thomas Conlon, Helen Herbert, Eva Campion, Nicolas Touzet
Publicado en: Process Biochemistry, Edición 155, 2025, Página(s) 117-127, ISSN 1359-5113
Editor: Elsevier Applied Science
DOI: 10.1016/j.procbio.2025.05.007

Effect of glycerol and H3PO4 on the bioactivity and degradability of rod-like SBA-15 particles with active surface for bone tissue engineering applications

Autores: Zulema Vargas-Osorio, Artem Klotschan, Marcela Arango-Ospina, Yolanda Piñeiro, Liliana Liverani, José Rivas, Martin Michálek, Dušan Galusek, Aldo R.Boccaccini
Publicado en: Microporous and Mesoporous Materials, Edición Volume 329, January 2022, 2021, Página(s) 111543, ISSN 1387-1811
Editor: Elsevier BV
DOI: 10.1016/j.micromeso.2021.111543

Isolation of Extracellular Vesicles From Microalgae: A Renewable and Scalable Bioprocess

Autores: Angela Paterna; Estella Rao; Giorgia Adamo; Samuele Raccosta; Sabrina Picciotto; Daniele Romancino; Rosina Noto; Nicolas Touzet; Antonella Bongiovanni; Mauro Manno
Publicado en: Frontiers in bioengineering and biotechnology - Sec. Nanobiotechnology, Edición Volume 10 - 14 March 2022, 2022, ISSN 2296-4185
Editor: Frontiers
DOI: 10.3389/fbioe.2022.836747

On the interaction and nanoplasmonics of gold nanoparticles and lipoproteins

Autores: Andrea Zendrini, Jacopo Cardellini, Roberto Frigerio, Marianna Bertoni, Debora Berti, Paolo Bergese
Publicado en: JCIS Open, Special issue for 11th Colloids Conference, Edición Volume 11, October 2023, Article 100088, 2023, ISSN 2666-934X
Editor: Elsevier
DOI: 10.1016/j.jciso.2023.100088

The biomolecular corona of extracellular nanoparticles holds new promises for advancing clinical molecular diagnostics

Autores: Annalisa Radeghieri and Paolo Bergese
Publicado en: Expert Review of Molecular Diagnostics, 2023, ISSN 1744-8352
Editor: Taylor & Francis
DOI: 10.1080/14737159.2023.2215927

Extracellular Vesicles Analysis in the COVID-19 Era: Insights on Serum Inactivation Protocols towards Downstream Isolation and Analysis

Autores: Roberto Frigerio, Angelo Musicò, Marco Brucale, Andrea Ridolfi, Silvia Galbiati, Riccardo Vago, Greta Bergamaschi, Anna Maria Ferretti, Marcella Chiari, Francesco Valle, Alessandro Gori, Marina Cretich
Publicado en: Cells, Edición 10/3, 2021, Página(s) 544, ISSN 2073-4409
Editor: Cells
DOI: 10.3390/cells10030544

Stiffness of Fluid and Gel Phase Lipid Nanovesicles: Weighting the Contributions of Membrane Bending Modulus and Luminal Pressurization

Autores: Andrea Ridolfi, Lucrezia Caselli, Matteo Baldoni, Costanza Montis, Francesco Mercuri, Debora Berti, Francesco Valle, Marco Brucale
Publicado en: Langmuir, 2021, ISSN 0743-7463
Editor: American Chemical Society
DOI: 10.1021/acs.langmuir.1c01660

Surface functionalization of extracellular vesicle nanoparticles with antibodies: a first study on the protein corona “variable”

Autores: Angelo Musicò, Rossella Zenatelli, Miriam Romano, Andrea Zendrini, Silvia Alacqua, Selene Tassoni, Lucia Paolini, Chiara Urbinati, Marco Rusnati, Paolo Bergese, Giuseppe Pomarico, and Annalisa Radeghieri
Publicado en: Nanoscale Advances, 5, Edición Edición 18, 2023, 2023, Página(s) 4703-4717, ISSN 2516-0230
Editor: ROYAL SOCIETY OF CHEMISTRY
DOI: 10.1039/d3na00280b

A plasmon-based nanoruler to probe the mechanical properties of synthetic and biogenic nanosized lipid vesicles

Autores: Lucrezia Caselli, Andrea Ridolfi, Jacopo Cardellini, Lewis Sharpnack, Lucia Paolini, Marco Brucale, Francesco Valle, Costanza Montis, Paolo Bergese, Debora Berti
Publicado en: Nanoscale Horizons, Edición 6/7, 2021, Página(s) 543-550, ISSN 2055-6756
Editor: Nanoscale Horizons
DOI: 10.1039/d1nh00012h

Extracellular vesicles from the microalga Tetraselmis chuii are biocompatible and exhibit unique bone tropism along with antioxidant and anti-inflammatory properties

Autores: Giorgia Adamo, Pamela Santonicola, Sabrina Picciotto, Paola Gargano, Aldo Nicosia, Valeria Longo, Noemi Aloi, Daniele P. Romancino, Angela Paterna, Estella Rao, Samuele Raccosta, Rosina Noto, Monica Salamone, Irene Deidda, Salvatore Costa, Caterina Di Sano, Giuseppina Zampi, Svenja Morsbach, Katharina Landfester, Paolo Colombo, Mingxing Wei, Paolo Bergese, Nicolas Touzet, Mauro Manno, Elia Di Schi
Publicado en: Communications Biology, Edición 7, 2024, ISSN 2399-3642
Editor: Communications Biology (Nature)
DOI: 10.1038/s42003-024-06612-9