Project description
Bioprinting bladder tissue
Certain conditions, including inflammation and cancer, require augmentation of the urinary bladder. However, the conventional approach of using bowel tissue suffers from risks and post-surgery complications. The EU-funded UroPrint project proposes to engineer bladder tissue for autologous transplantation using laser-induced forward transfer (LIFT) bioprinting technology. Using a prototype bioprinter, researchers will graft primary urothelial and smooth muscle cells onto a natural scaffold ensuring high cell viability and mimicking the function of the human bladder. UroPrint is expected to improve clinical outcomes and the quality of life of patients by reducing hospitalisation.
Objective
A number of conditions, including trauma, inflammation, incontinence, overactive bladder, renal impairments, neurological disorders (like spinal cord injury or spina bifida) and cancer, require bladder augmentation. For almost a century now, the majority of cystoplasties utilize bowel segments (enterocystoplasty). This, almost a century years old, gold standard practice bears numerous risks and complications affecting the majority of patients, thus compromising the quality of life while burdening the health care systems. This has fuelled efforts towards the development of engineered bladder tissue. Advancements in bioprinting technologies are increasingly employed in regenerative medicine but mostly in smaller and less complicated tissues. UroPrint proposes the use of Laser Induced Forward Transfer (LIFT) to generate bladder tissue for autologous transplantation that would meet the biological, mechanical and functional properties of human bladder. To this end, primary urothelial and smooth muscle cells will be obtained from healthy donors and expanded in fully Good Medical Practice compliant methodologies. These will be combined with novel natural autologous scaffold material obtained from platelet lysates. Then, a novel approach in the generation of bladder transplant will be utilized, combining intestine denudation and in vivo printing during surgery using a novel prototype LIFT printer that achieved high spatial resolution (<10 μm), high cell viability (>95%) single-urothelial cells.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. This project's classification has been validated by the project's team.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. This project's classification has been validated by the project's team.
Keywords
Programme(s)
Call for proposal
(opens in new window) H2020-FETOPEN-2018-2020
See other projects for this callSub call
H2020-FETOPEN-2018-2019-2020-01
Funding Scheme
RIA - Research and Innovation actionCoordinator
115 27 Athina
Greece