Objetivo THE TECHNIQUES OF DNA SEQUENCING ARE OF CENTRAL IMPORTANCE IN MOLECULAR BIOLOGY AND BIOTECHNOLOGY. DETERMINATION OF THE COMPLETE NUCLEOTIDE SEQUENCES OF LARGE MOLECULES BY THE PRESENT MANUAL METHODS IS, HOWEVER, A TEDIOUS AND TIME CONSUMING PROCESS AS ARE THE ITERATIVE MANUAL DETERMINATIONS OF RELATIVELY SHORT SEQUENCES.THE REPETITIVE NATURE OF THE TASK MAKES IT AN IDEAL SUBJECT FOR AUTOMATION. THE AUTOMATION OF THE PROCESS AIMED AT IN THE PRESENT PROPOSAL SHOULD SPEED UP THE DETERMINATION OF NUCLEOTIDE SEQUENCES FOR THE BENEFIT OF BOTH ACADEMIC AND INDUSTRIAL RESEARCH. Deoxyribonucleic acid (DNA) sequencing is an obvious candidate for automation allowing more rapid access to the genetic data encoded in very large DNA molecules, such as the human genome.Research was carried out into the production of fast and accurate instrument modules to automate aspects of sequence analysis. A novel machine was designed and built which incorporated a vision controlled robot to identify and select plaques and colonies on Petri dishes for automatic sampling and transfer of candidate material for further growth in individual containers. Electrophoresis and direct blotting were automated and a gas counter was built for directly imaging radiolabelled DNA sequences and mapping gels for genetic data abstraction.The first robot system (APSCIR) to image mixed randomly disposed arrays of biological specimens and to pick a particular set of these for distribution into an ordered arrangement was produced. Imaging hardware and software were developed jointly with a patented robot picking head to automate this process, which is recognized as a serious bottleneck in many DNA analysis procedures. The direct blotting electrophoresis system was developed into a key technology for the second generation of automatic mappers and sequencers and the gas counter capabilities were extended into the area of sequence and genetic analysis.DEVELOPMENT OF A FAST AND USER-FRIENDLY VISION SYSTEM FOR THE AUTOMATIC RECOGNITION AND LOCATION OF PLAQUES BY COLOUR, SHAPE AND PATTERN AND THE GUIDANCE OF A PICK-UP ROBOT TO PLAQUES CORRESPONDING TO A GIVEN SET OF USER-SELECTABLE CRITERIA. Ámbito científico natural sciencesbiological sciencesgeneticsDNAsocial sciencessociologyindustrial relationsautomationnatural sciencesbiological sciencesgeneticsnucleotidesnatural scienceschemical scienceselectrochemistryelectrophoresisnatural sciencesbiological sciencesmolecular biology Programa(s) FP1-BAP - Multiannual research action programme (EEC) in the field of biotechnology (BAP), 1985-1989 Tema(s) Data not available Convocatoria de propuestas Data not available Régimen de financiación CSC - Cost-sharing contracts Coordinador Steinbeis-Transferzentrum System- und Softwareengineering Aportación de la UE Sin datos Dirección Reichenaustraße 81c 78467 Konstanz Alemania Ver en el mapa Coste total Sin datos Participantes (3) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo Christian-Albrechts-Universität Alemania Aportación de la UE Sin datos Dirección Olshausen str. 40 24098 Kiel Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos Rutherford Appleton Laboratory (RAL) Reino Unido Aportación de la UE Sin datos Dirección Chilton OX11 0QX Didcot Ver en el mapa Coste total Sin datos University of Manchester Institute of Science and Technology (UMIST) Reino Unido Aportación de la UE Sin datos Dirección Sackville Street M60 1QD Manchester Ver en el mapa Coste total Sin datos