Objective The overall objectives of the INTEGRA project is to develop new genetic engineering tools that will permit targeted integration of a transgene into the genome of the desired eukaryotic organism. This project will allow the easy and rapid generation of transgenic knock out or knock in small and large animals without the need of embryonic stem cells. This will have visible repercussions on fundamental and applied research particularly in pharmaceutics agronomy and food sciences.At the same time it will strengthen European research in the field of gene therapy with the development of safe viral vectors based on non integrative or targeted integration vectors adequate for clinical use. We plan to meet the objectives by two alternative means. The first one will combine the use of a lentiviral episomal vector with a phage site specific integration system to direct the integration of the transgene into a unique locus in the host genome. The alternative method will use lentiviral vectors for which the integrase specificity will be modified HIV integrases will be generated whose DNA binding domain will be replaced by a synthetic DNA binding domain able to recognize any given DNA sequence of 9 or 18 base pairs. Such specific integrase will be incorporated into a gene transfer vector system that will allow the genetic modification of somatic cells and zygotes.These technologies will be used by the partners to generate transgenic livestock with modified traits improved growth traits disease resistance and to generate sensor animals to monitor contamination of livestock by toxic agents. On the other hand gene therapy preclinical studies will be performed using the secured vectors Specific ethical and societal implications of the project will be anticipated through the participation of European stakeholders. The overall INTEGRA project clearly cuts across the Thematic Priority 1 and 5 and the Science and Society programme of FP6. Fields of science agricultural sciencesagriculture, forestry, and fisheriesagricultureagronomy Keywords Knock Knock out livestock and ethics food food safety livestock out recombinase safety sciences site social social sciences and ethics specific specific recombinase targeted targeted site Programme(s) FP6-POLICIES - Policy support: Specific activities covering wider field of research under the Focusing and Integrating Community Research programme 2002-2006. Topic(s) NEST-2004-ADV - Adventure activities Call for proposal FP6-2004-NEST-C-1 See other projects for this call Funding Scheme STREP - Specific Targeted Research Project Coordinator CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE EU contribution No data Address 3 rue Michel Ange PARIS France See on map Links Website Opens in new window Total cost No data Participants (8) Sort alphabetically Sort by EU Contribution Expand all Collapse all CENTRE DE REGULACIO GENOMICA Spain EU contribution No data Address Passeig Maritim, 37-49 BARCELONA See on map Links Website Opens in new window Total cost No data ROSLIN INSTITUTE United Kingdom EU contribution No data Address Roslin Midlothian ROSLIN See on map Links Website Opens in new window Total cost No data THE UNIVERSITY OF COPENHAGEN Denmark EU contribution No data Address Norregade 10 COPENHAGEN See on map Links Website Opens in new window Total cost No data FONDATION ASILE DES AVEUGLES Switzerland EU contribution No data Address 15, av. de France LAUSANNE See on map Links Website Opens in new window Total cost No data THE UNIVERSITY OF EDINBURGH United Kingdom EU contribution No data Address Old College, South Bridge EDINBURGH See on map Links Website Opens in new window Total cost No data INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE France EU contribution No data Address 101 rue de Tolbiac PARIS See on map Links Website Opens in new window Total cost No data UNIVERSITÉ DE STRASBOURG France EU contribution No data Address Blaise Pascal 4 STRASBOURG See on map Links Website Opens in new window Total cost No data UNIVERSITÉ PARIS SUD XI France EU contribution No data Address 15 rue Georges Clémenceau ORSAY See on map Links Website Opens in new window Total cost No data
