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Reinventing a smart, circular and competitive textile industry with advanced myco-fibres

Periodic Reporting for period 3 - MY-FI (Reinventing a smart, circular and competitive textile industry with advanced myco-fibres)

Berichtszeitraum: 2023-11-01 bis 2024-10-31

The MY-FI project set out to develop an innovative, high-value, and environmentally responsible biobased material. By leveraging a biobased approach, MY-FI transformed low-value substrates into valuable materials using mild, eco-friendly processes designed to minimize resource inputs—such as energy, water, land, and chemicals—while reducing waste and avoiding toxic compounds to ensure safer manufacturing practices.
The project employed two biofabrication technologies, which involved collaborating with the mycelium, as a pivotal living partner. This process enables the binding and transformation of substrates into a novel class of materials with broad applications, including the fashion and automotive industries.
In particular, MY-FI successfully reached pilot scale for both processes, demonstrating significant progress. The Surface Liquid Fermentation achieved a pilot-scale production capacity of 1,900 m2 of material per year, meeting many industrial standards for both fashion and automotive applications. Moreover, 14 prototypes have been developed in the fashion and automotive sectors. The process proved particularly successful, showcasing the potential to grow mycelium-based materials in virtually any size and shape with minimal waste. Its sustainable, scalable, and cost-competitive nature makes it suitable for industrial-level production.
In the meantime, the Dynamic Liquid Fermentation process reached a scale of 1,500L but the resulting material did not yet achieve sufficient quality for broad applications. However, the process demonstrated clear potential for upscaling, laying the groundwork for further research to improve material properties.
In general,the Surface Liquid Fermentation process has opened doors to a new generation of biobased materials and an emerging industry in Europe, reducing dependency on non-EU countries by utilizing local, residual, and regenerative resources. Beyond its environmental benefits, the MY-FI project fosters job creation in rural areas tied to the bioeconomy. In addition to its technological advancements, the project developed policy guidelines for decision-makers to support the growth of biobased industries that have been translated into six languages (English, German, French, Italian, Spanish, Romanian) (Annex of D8.6).
MY-FI successfully upscaled biofabrication processes and tested the materials across different sectors. Over the past year, the project focused on finalizing the upscaling of the Dynamic Liquid Fermentation protocol and analyzing the properties of various materials, including the one from the Surface Liquid Fermentation. The Dynamic Liquid Fermentation process reached a scale of 1,500L, despite the low quality of the material. Further experimentation is required to achieve a high-quality product. Meanwhile, the Surface Liquid Fermentation material demonstrated satisfactory performance and significant advancements compared to the raw material, making it suitable for prototyping activities in both the fashion and automotive industries. The project developed 12 prototypes in the fashion industry and 2 in the automotive industry.
Thanks to improvements in the wet process, water usage has been reduced, and a wastewater treatment system has been implemented. This supports the industrial scale-up of the Surface Liquid Fermentation process and its market exploitation. The pilot scale of 1,900m²/year has been successfully achieved, paving the way for industrial upscaling.
Biodegradability tests were conducted on the finished material. Both Dynamic and Surface Liquid Fermentation materials were tested under industrial composting and marine conditions. Additionally, partners evaluated microplastic release; however, the test had several limitations, preventing a fully reliable analysis of the materials. The Life Cycle Assessment (LCA) and Cost Analysis (LCC) for both processes, including wet processing, were also performed. The LCA results were highly promising, while the LCC highlighted the need for greater efficiency and economies of scale. Further research is required to achieve full material circularity.
IPR management and an extended patent analysis were conducted, covering all processes and products developed within MY-FI. Additionally, the project finalized 19 Key Exploitable Results (KERs). Business Plans for all partners were completed, and for industrial partners, a Financial Analysis was carried out, estimating CAPEX and OPEX, which contributed to the Profit and Loss analysis. The results indicate promising potential for future investment. To complete the Business Landscape, Market Segmentation and Competitor Analysis were included, providing a comprehensive view of the market, its segmentation, competitors, and their activities. The market entry of mycelium-based materials represents the largest share among next-generation plant-derived materials and is expected to grow.
The final MY-FI conference took place in Milan during the ECOSYSTEX Conference, where the prototypes were showcased. BOND created over 12 prototypes, including complex designs such as a jacket and a dress, while CRF and VW AG developed a headrest and a dashboard fascia. The Surface Liquid Fermentation material demonstrated its versatility across industries and its potential for market entry.
Three scientific papers have been published, and Guidelines for policymakers have also been released. The project has been widely disseminated through the MY-FI website, social media channels, newsletters, and participation in conferences and events. The Stakeholder Advisory Board, comprising key stakeholders, has met three times since the project’s inception, focusing on key results related to biofabrication, post-processing, and prototyping.
The main takeaway is that the material is highly promising for the market, with stakeholders considering it a strong starting point for a new generation of materials across various industries. The tactile and visual qualities of the raw material are particularly noteworthy and should be further emphasized.
MY-FI went beyond the state of the art, demonstrating that:
- Biofabrication processes are innovative, requiring low water, energy, and land use, no chemicals, and can be upscaled in different and remote areas by valorizing low-value substrates.
- These new materials can be used in high-demand value chains such as fashion and automotive.
- The processes—particularly Surface Liquid Fermentation—can be scaled up to an industrial level.
- There is significant market potential for these materials.
- Consumers are increasingly demanding novel materials, and the industry is ready to meet these needs.
- Brands and designers are eager to adopt new materials.
- These novel materials offer a competitive advantage in terms of environmental performance and social/ethical impact.
- These new processes fosters job creation in rural areas tied to the bioeconomy

MY-FI marks the beginning of a new era of bio-based materials rooted in biological processes. They have the potential to transform the economy by making supply chains more local and ethical. This revolution requires industry engagement, and MY-FI has demonstrated that the sector is ready to take part.
Bioreactor used for mycelium dynamic liquid fermentation
Raw mycelium material obtained with surface liquid fermentation
Raw mycelium material obtained with dynamic liquid fermentation
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