HARNESSING THE POWER OF NATURE THROUGH PRODUCTIVE MICROBIAL CONSORTIA IN BIOTECHNOLOGY – MEASURE, MODEL, MASTER

Project Information

PROMICON

Grant agreement ID: 101000733

DOI

10.3030/101000733

Project closed

EC signature date 3 May 2021

Start date 1 June 2021

End date 31 May 2025

Funded under

• SOCIETAL CHALLENGES - Food security, sustainable agriculture and forestry, marine, maritime and inland water research, and the bioeconomy

Total cost

€ 5 999 712,50

EU contribution

€ 5 999 712,50

Coordinated by

HELMHOLTZ-ZENTRUM FUR UMWELTFORSCHUNG GMBH - UFZ
Germany

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Documents, reports (27)

Selection and characterization of microbiomes under ecological stress
Running on-line single cell analysis platform

Running online single cell analysis platform

A set of optimized P. putida strains overproducing PHACOS from sucrose and 6-ATH.
Project Collection of OMICS data in the Research Ideas and Outcomes journal

Project collection of papers and datasets published in Research Ideas and Outcomes Journal

A set of in silico optimized synthetic microbial consortia overproducing PHA, butanol and H2 from CO2 and sunlight.
Report about Multispectral characterization of microbiome: PHA, EPS, biomass and phycobiliproteins
Rationally engineered 3D Synechococcus/P. putida PHA-producing consortium.
PROMICON Economic Analysis

Report on PROMICON Economic Analysis -intermediate (m24) and final (m48)

SOPs for the operation of the capillary biofilm reactor available for the PROMICON consortium
Quantitative MS-based metabolomics dataset

Quantitative MSbased metabolomics dataset

Future recommendations factsheets based on a detailed report and evaluation of dissemination and communication activities
Modelling of chemotrophs and cyanobacterial-rich microbiomes at (meta)genome-scale.
PROMICON LCA
Report on the phototrophs and chemotrophs optimal conditions for PPP, EPS and PHA production based on WP1 models
Connecting on-line single cell data with automatic evaluation pipelines

Connecting online single cell data with automatic evaluation pipelines

Sucrose overproducer Synechococcus strain based on deletion of competing pathways and population homogenization.
Report and user manual of AI models for measurement of M1.3 parameters from spectral images
A set of optimized P. taiwanensis VLB 120 balancer strains with enhanced c-di-GMP levels.

A set of optimized P taiwanensis VLB 120 balancer strains with enhanced cdiGMP levels

NMR-environomics dataset

NMRenvironomics dataset

Rationally engineered consortium for continuous photofermentative H2 production.
SOPs for the operation of the dual chamber system available for the PROMICON consortium
Meta-functional-environomics reconstruction of natural microbiome

Metafunctionalenvironomics reconstruction of natural microbiome

Characterization of microbial consortia from natural environments
PROMICON S-LCA
A P. putida strain suitable as labor module including sucrose metabolism and lacking negative traits.

A P putida strain suitable as labor module including sucrose metabolism and lacking negative traits

Hybrid AI/physical controller of natural microbiomes
In situ analysis of the biofilm microbiome growing in a CBR accomplished

Websites, patent fillings, videos etc. (1)

Project branding and communication channels set-up and running (logo, promotional materials, website, templates, social media)

Project branding and communication channels setup and running logo promotional materials website templates social media

Demonstrators, pilots, prototypes (1)

Proof of concept shown for operating a dual chamber membrane reactor for the production of PHACOS

Publications

Peer reviewed articles (30)

Development of an Automated Online Flow Cytometry Method to Quantify Cell Density and Fingerprint Bacterial Communities

Author(s): Juan López-Gálvez, Konstanze Schiessl, Michael D. Besmer, Carmen Bruckmann, Hauke Harms, Susann Müller
Published in: Cells, Issue 12, 2023, Page(s) 1559, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/cells12121559

New strategy for bioplastic and exopolysaccharides production: Enrichment of field microbiomes with cyanobacteria

Author(s): Beatriz Altamira-Algarra, Estel Rueda, Artai Lage, David San León, Juan F. Martínez-Blanch, Juan Nogales, Joan García, Eva Gonzalez-Flo
Published in: New Biotechnology, Issue 78, 2023, Page(s) 141-149, ISSN 1871-6784
Publisher: Elsevier BV
DOI: 10.1016/j.nbt.2023.10.008

Enhancing pigment production with cyanobacteria-rich microbiomes: Effect of light quality and exposure time

Author(s): Marta Bellver, Beatriz Altamira-Algarra, Joan García, Ivet Ferrer, Eva Gonzalez-Flo
Published in: Algal Research, Issue 83, 2024, Page(s) 103726, ISSN 2211-9264
Publisher: Elsevier BV
DOI: 10.1016/j.algal.2024.103726

Monitoring PHB production in Synechocystis sp. with hyperspectral images

Author(s): Francisco Rodríguez Lorenzo; Miguel Placer Lorenzo; Luz Herrero Castilla; Juan Antonio Álvarez Rodríguez; Sandra Iglesias; Santiago Gómez; Juan Manuel Fernández Montenegro; Estel Rueda; Rubén Diez-Montero; Joan Garcia; Eva Gonzalez-Flo
Published in: Water Science & Technology, 2022, ISSN 1996-9732
Publisher: IWA Publishing
DOI: 10.2166/wst.2022.194

Bioplastic production by harnessing cyanobacteria-rich microbiomes for long-term synthesis

Author(s): Beatriz Altamira-Algarra, Artai Lage, Ana Lucía Meléndez, Marc Arnau, Eva Gonzalez-Flo, Joan García
Published in: Science of The Total Environment, Issue 954, 2025, Page(s) 176136, ISSN 0048-9697
Publisher: Elsevier BV
DOI: 10.1016/j.scitotenv.2024.176136

Novel approaches to energize microbial biocatalysts

Author(s): Gonzalo Durante‐Rodríguez, Manuel Carmona, Eduardo Díaz
Published in: Environmental Microbiology, Issue 25, 2023, Page(s) 161-166, ISSN 1462-2912
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.16254

Toward merging bottom–up and top–down model-based designing of synthetic microbial communities

Author(s): David San León & Juan Nogales
Published in: Current Opinion in Microbiology, 2022, ISSN 1369-5274
Publisher: Elsevier BV
DOI: 10.1016/j.mib.2022.102169

Photosynthetic species composition determines bioplastics production in microbiomes: Strategy to enrich cyanobacteria PHB-producers

Author(s): Beatriz Altamira-Algarra, Artai Lage, Joan García, Eva Gonzalez-Flo
Published in: Algal Research, Issue 79, 2024, Page(s) 103469, ISSN 2211-9264
Publisher: Elsevier BV
DOI: 10.1016/j.algal.2024.103469

Cyanobacteria microbiomes for bioplastic production: Critical review of key factors and challenges in scaling from laboratory to industry set-ups

Author(s): Beatriz Altamira-Algarra, Joan Garcia, Eva Gonzalez-Flo
Published in: Bioresource Technology, Issue 422, 2025, Page(s) 132231, ISSN 0960-8524
Publisher: Elsevier BV
DOI: 10.1016/j.biortech.2025.132231

A double-staining automated flow cytometry method for real-time monitoring of bacteria in continuous bioreactors

Author(s): Juan López-Gálvez, Erik Schönfelder, Hanna Mayer, Konstanze Schiessl, Marisa O. D. Silva, Hauke Harms, Susann Müller
Published in: A double-staining automated flow cytometry method for real-time monitoring of bacteria in continuous bioreactors, 2025, ISSN 2692-8205
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2025.03.24.644962

Molecular dynamics of photosynthetic electron flow in a biophotovoltaic system

Author(s): Jianqi Yuan, Jens Appel, Kirstin Gutekunst, Bin Lai, Jens Olaf Krömer
Published in: Environmental Science and Ecotechnology, Issue 23, 2025, Page(s) 100519, ISSN 2666-4984
Publisher: Elsevier BV
DOI: 10.1016/j.ese.2024.100519

Improving PHB production in cyanobacteria: Modeling the optimal light regime for growth

Author(s): I. Perez Couñago, J.M. Fernandez Montenegro, S. Iglesias Moreira, F. Rodriguez Lorenzo, M. Placer Lorenzo, P. Villar Sola, E. Pancorbo González, J. Illade Quinteiro, L. Herrero Castilla, J.A. Álvarez Rodríguez, B. Altamira Algarra, E. Gonzalez Flo, J. Garcia, F. Guedes, M. Lopez-Garcia, S. Muiños-Landin
Published in: New Biotechnology, Issue 89, 2025, Page(s) 73-81, ISSN 1871-6784
Publisher: Elsevier BV
DOI: 10.1016/j.nbt.2025.05.005

Exploring and increased acetate biosynthesis in Synechocystis PCC 6803 through insertion of a heterologous phosphoketolase and overexpressing phosphotransacetylase

Author(s): Stamatina Roussou, Minmin Pan, Jens O. Krömer, Peter Lindblad
Published in: Metabolic Engineering, Issue 88, 2025, Page(s) 250-260, ISSN 1096-7176
Publisher: Academic Press
DOI: 10.1016/j.ymben.2025.01.008

Exploiting synergies between microbial electrochemical technologies and synthetic biology

Author(s): Bin Lai, Jens Krömer, Federico Aulenta, Hui Wu, Pablo Ivan Nikel
Published in: Microbial Biotechnology, Issue 16, 2023, Page(s) 485-488, ISSN 1751-7915
Publisher: Wiley
DOI: 10.1111/1751-7915.14208

Robust strategy for bioplastic production from cyanobacteria-enriched microbiomes: insights from gene expression and population dynamics

Author(s): Beatriz Altamira-Algarra, Lin Sun, David San León Granado, Lucía Romero-Morillo, Livia Vurro, Juan Nogales, Eva Gonzalez-Flo, Joan Garcia
Published in: Chemical Engineering Journal, Issue 517, 2025, Page(s) 164196, ISSN 1385-8947
Publisher: Elsevier BV
DOI: 10.1016/j.cej.2025.164196

Rational Design Assisted by Evolutionary Engineering Allows <scp>(De)Construction</scp> and Optimization of Complex Phenotypes in <scp><i>Pseudomonas putida</i> KT2440</scp>

Author(s): Blas Blázquez, Juan Nogales
Published in: Microbial Biotechnology, Issue 18, 2025, ISSN 1751-7915
Publisher: Wiley
DOI: 10.1111/1751-7915.70132

When microbial biotechnology meets material engineering

Author(s): Ana M. Hernández-Arriaga, Cristina Campano, Virginia Rivero-Buceta, M. Auxiliadora Prieto
Published in: Microbial Biotechnology, 2022, ISSN 1751-7915
Publisher: Willey
DOI: 10.1111/1751-7915.13975

A general deep hybrid model for bioreactor systems: Combining first principles with deep neural networks

Author(s): José Pinto, Mykaella Mestre, J.Ramos, Rafael S.Costa, Gerald Striedner, RuiOliveira
Published in: Computers & Chemical Engineering, 2022, ISSN 0098-1354
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.compchemeng.2022.107952

Cyanobacterial biofilms: from natural systems to applications

Author(s): Mahir Bozan, Hannah Berreth, Pia Lindberg, Katja Bühler
Published in: Trends in Biotechnology, 2024, ISSN 0167-7799
Publisher: Elsevier BV
DOI: 10.1016/j.tibtech.2024.08.005

Challenges, progress, and future perspectives for cyanobacterial polyhydroxyalkanoate production

Author(s): Estel Rueda, Eva Gonzalez-Flo, Soumila Mondal, Karl Forchhammer, Dulce María Arias, Katharina Ludwig, Bernhard Drosg, Ines Fritz, C. Raul Gonzalez-Esquer, Sara Pacheco, Joan García
Published in: Reviews in Environmental Science and Bio/Technology, Issue 23, 2024, Page(s) 321-350, ISSN 1569-1705
Publisher: Kluwer Academic Publishers
DOI: 10.1007/s11157-024-09689-0

A Coculture of Photoautotrophs and Hydrolytic Heterotrophs Enables Efficient Upcycling of Starch from Wastewater toward Biomass-Derived Products: Synergistic Interactions Impacting Metabolism of the Consortium

Author(s): Minmin Pan, Yiru Wang, Jens O. Krömer, Xinyu Zhu, Marie Karen Tracy Hong Lin, Irini Angelidaki
Published in: Environmental Science &amp; Technology, Issue 57, 2023, Page(s) 15523-15532, ISSN 0013-936X
Publisher: American Chemical Society
DOI: 10.1021/acs.est.3c05321

SBML2HYB: a Python interface for SBML compatible hybrid modeling

Author(s): José Pinto, Rafael S Costa, Leonardo Alexandre, João Ramos, Rui Oliveira
Published in: Bioinformatics, 2023, ISSN 1367-4803
Publisher: Oxford University Press
DOI: 10.1093/bioinformatics/btad044

Exploring simultaneous production of poly(3-hydroxybutyrate) and exopolysaccharides in cyanobacteria-rich microbiomes

Author(s): Beatriz Altamira-Algarra, Joan García, Cristiana A.V. Torres, Maria A.M. Reis, Eva Gonzalez-Flo
Published in: New Biotechnology, Issue 87, 2025, Page(s) 82-92, ISSN 1871-6784
Publisher: Elsevier BV
DOI: 10.1016/j.nbt.2025.02.008

Enhancing biodesulfurization by engineering a synthetic dibenzothiophene mineralization pathway

Author(s): Igor Martínez, Magdy El-Said Mohamed, José Luis García, Eduardo Díaz
Published in: Frontiers in Microbiology, Issue 13, 2022, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2022.987084

Engineering a Photoautotrophic Microbial Coculture toward Enhanced Biohydrogen Production

Author(s): Minmin Pan, Rodrigo Amarante Colpo, Stamatina Roussou, Chang Ding, Peter Lindblad, Jens O. Krömer
Published in: Environmental Science &amp; Technology, Issue 59, 2025, Page(s) 337-348, ISSN 0013-936X
Publisher: American Chemical Society
DOI: 10.1021/acs.est.4c08629

Ecological forces dictate microbial community assembly processes in bioreactor systems

Author(s): Shuang LiSusann Müller
Published in: Current Opinion in Biotechnology, 2023, ISSN 0958-1669
Publisher: Elsevier BV
DOI: 10.1016/j.copbio.2023.102917

Current advances in engineering cyanobacteria and their applications for photosynthetic butanol production

Author(s): Xufeng Liu, Hao Xie, Stamatina Roussou, Peter Lindblad
Published in: Current Opinion in Biotechnology, 2021, ISSN 0958-1669
Publisher: Elsevier BV
DOI: 10.1016/j.copbio.2021.07.014

Microalgae harvesting for wastewater treatment and resources recovery: A review

Author(s): Etiele Greque de Morais, Igor Carvalho Fontes Sampaio, Eva Gonzalez-Flo, Ivet Ferrer, Enrica Uggetti, Joan García
Published in: New Biotechnology, Issue 78, 2023, Page(s) 84-94, ISSN 1871-6784
Publisher: Elsevier BV
DOI: 10.1016/j.nbt.2023.10.002

Enhancing tellurite and selenite bioconversions by overexpressing a methyltransferase from <i>Aromatoleum</i> sp. CIB

Author(s): Elena Alonso‐Fernandes, Helga Fernández‐Llamosas, Irene Cano, Cristina Serrano‐Pelejero, Laura Castro, Eduardo Díaz, Manuel Carmona
Published in: Microbial Biotechnology, Issue 16, 2023, Page(s) 915-930, ISSN 1751-7915
Publisher: Willey
DOI: 10.1111/1751-7915.14162

New Insights on Metabolic Features of Bacillus subtilis Based on Multistrain Genome-Scale Metabolic Modeling

Author(s): Blas Blázquez, David San León, Antonia Rojas, Marta Tortajada, Juan Nogales
Published in: International Journal of Molecular Sciences, Issue 24, 2023, Page(s) 7091, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms24087091