Compact Cell-Imaging Device to provide insight into the cellular origins of diseases and to aid in the development of novel therapeutics

Informazioni relative al progetto

CoCID

ID dell’accordo di sovvenzione: 101017116

DOI

10.3030/101017116

Progetto chiuso

Data della firma CE 4 Dicembre 2020

Data di avvio 1 Gennaio 2021

Data di completamento 31 Maggio 2025

Finanziato da

• INDUSTRIAL LEADERSHIP - Leadership in enabling and industrial technologies - Information and Communication Technologies (ICT)

Costo totale

€ 5 686 215,00

Contributo UE

€ 5 686 215,00

Coordinato da

UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLIN
Ireland

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Open Research Data Pilot (6)

Imaging of HEV-infected ex vivo porcine precision cut liver slices
Structural differences in HEV-infected pig and human cell lines
Correlation of the kinetics normal cell homeostasis of viral antigen and genomic RNA with restoration of

Correlation of the kinetics of viral antigen and genomic RNA with restoration of normal cell homeostasis

3D model of infected lung epithelial cell
Calibration table of x-ray absorption coefficient for adherent cells.

The table will provide calibration value to translated x-ray linear absorption coefficient obtained with lab-based SXM with respect to the synchrotron source for EM grid sample holders and limited angle tomography.

Calibration table of x-ray absorption coefficient for suspended cells.

The table will provide calibration value to translated x-ray linear absorption coefficient obtained with lab-based SXM with respect to the synchrotron source for cells in suspension and full rotation tomography.

Demonstrators, pilots, prototypes (1)

Fully tested in-line FM

Fully validated FM as indicated through bench tests and inline imaging of fluorescent nanospheres

Pubblicazioni

Peer reviewed articles (32)

Gap junctions and connexin hemichannels both contribute to the electrical properties of retinal pigment epithelium

Autori: Julia Fadjukov, Sophia Wienbar, Satu Hakanen, Vesa Aho, Maija Vihinen-Ranta, Teemu O. Ihalainen, Gregory W. Schwartz, Soile Nymark
Pubblicato in: Journal of General Physiology, Numero 154, 2023, ISSN 0022-1295
Editore: Rockefeller University Press
DOI: 10.1085/jgp.202112916

Lipid monitoring of Chlorella vulgaris using non-invasive near-infrared spectral imaging

Autori: Salli Pääkkönen, Ilkka Pölönen, Marco Calderini, Aliisa Yli-Tuomola, Visa Ruokolainen, Maija Vihinen-Ranta, Pauliina Salmi
Pubblicato in: Journal of Applied Phycology, Numero 37, 2025, Pagina/e 205-219, ISSN 0921-8971
Editore: Kluwer Academic Publishers
DOI: 10.1007/s10811-024-03397-6

Soft X-Ray Tomography Has Evolved into a Powerful Tool for Revealing Cell Structures

Autori: Venera Weinhardt, Carolyn Larabell
Pubblicato in: Annual Review of Analytical Chemistry, 2025, ISSN 1936-1327
Editore: Annual Reviews, Inc.
DOI: 10.1146/annurev-anchem-071124-093849

Three-Dimensional Remodeling of SARS-CoV2-Infected Cells Revealed by Cryogenic Soft X-ray Tomography

Autori: Victoria Castro, Ana Joaquina Pérez-Berna, Gema Calvo, Eva Pereiro*, and Pablo Gastaminza
Pubblicato in: ACS Nano, Numero 2023, 17, 22, 2023, Pagina/e 22708–22721, ISSN 1936-0851
Editore: American Chemical Society
DOI: 10.1021/acsnano.3c07265

Iterative immunostaining combined with expansion microscopy and image processing reveals nanoscopic network organization of nuclear lamina

Autori: Elina Mäntylä, Toni Montonen, Lucio Azzari, Salla Mattola, Markus Hannula, Maija Vihinen-Ranta, Jari Hyttinen, Minnamari Vippola, Alessandro Foi, Soile Nymark, Teemu O. Ihalainen
Pubblicato in: Molecular Biology of the Cell, Numero 34, 2023, ISSN 1059-1524
Editore: American Society for Cell Biology
DOI: 10.1091/mbc.e22-09-0448

Creation of ordered 3D tubes out of DNA origami lattices

Autori: Johannes M. Parikka, Heini Järvinen, Karolina Sokołowska, Visa Ruokolainen, Nemanja Markešević, Ashwin K. Natarajan, Maija Vihinen-Ranta, Anton Kuzyk, Kosti Tapio, J. Jussi Toppari
Pubblicato in: Nanoscale, Numero 15, 2024, Pagina/e 7772-7780, ISSN 2040-3364
Editore: Royal Society of Chemistry
DOI: 10.1039/d2nr06001a

Hepatitis C virus‐induced differential transcriptional traits in host cells after persistent infection elimination by direct‐acting antivirals in cell culture

Autori: Victoria Castro, Gema Calvo, Juan Carlos Oliveros, Sofía Pérez‐del‐Pulgar, Pablo Gastaminza
Pubblicato in: Journal of Medical Virology, Numero 96, 2024, ISSN 0146-6615
Editore: John Wiley & Sons Inc.
DOI: 10.1002/jmv.29787

Herpesvirus-Induced Manipulation of the Nucleolus

Autori: Simon Leclerc, Sergey Kapishnikov, Visa Ruokolainen, Inka Huusko, Salla Mattola, David Rogers, Stephen O´Connor, Vesa Aho, Kenneth Fahy, M Vihinen-Ranta
Pubblicato in: Microscopy and Microanalysis, Numero 30, 2024, ISSN 1431-9276
Editore: Cambridge University Press
DOI: 10.1093/mam/ozae044.1050

Monitoring reversion of hepatitis C virus-induced cellular alterations by direct-acting antivirals using cryo soft X-ray tomography and infrared microscopy

Autori: Ana J. Pérez-Berná; Nuria Benseny-Cases; María José Cubillas Rodríguez; Ricardo Valcárcel; José L. Carrascosa; Pablo Gastaminza; Eva Pereiro
Pubblicato in: Acta Crystallographica Section D: Structural Biology, Numero 1, 2021, ISSN 2059-7983
Editore: IUCr
DOI: 10.1107/s2059798321009955

Infection-induced chromatin modifications facilitate translocation of herpes simplex virus capsids to the inner nuclear membrane

Autori: Vesa Aho ,Sami Salminen, Salla Mattola, Alka Gupta, Felix Flomm, Beate Sodeik, Jens B. Bosse, Maija Vihinen-Ranta
Pubblicato in: PLOS PATHOGENS, 2021, ISSN 1553-7366
Editore: Public Library of Science
DOI: 10.1371/journal.ppat.1010132

A coronavirus assembly inhibitor that targets the viral membrane protein

Autori: Manon Laporte, Dirk Jochmans, Dorothée Bardiot, Lowiese Desmarets, Oliver J. Debski-Antoniak, Giulia Mizzon, Rana Abdelnabi, Pieter Leyssen, Winston Chiu, Zhikuan Zhang, Norimichi Nomura, Sandro Boland, Umeharu Ohto, Yannick Stahl, Jurgen Wuyts, Steven De Jonghe, Annelies Stevaert, Martijn J. van Hemert, Brenda W. Bontes, Patrick Wanningen, G. J. Mirjam Groenewold, Aneta Zegar, Katarzyna Owczarek
Pubblicato in: Nature, Numero 640, 2025, Pagina/e 514-523, ISSN 0028-0836
Editore: Nature Publishing Group
DOI: 10.1038/s41586-025-08773-x

Soft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells

Autori: Jian-Hua Chen,Bieke Vanslembrouck, Axel Ekman, Vesa Aho, Carolyn A. Larabell, Mark A. Le Gros, Maija Vihinen-Ranta, Venera Weinhardt
Pubblicato in: Viruses, 2022, ISSN 1999-4915
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v14122651

Soft X-ray tomography reveals variations in B. subtilis biofilm structure upon tasA deletion

Autori: Anthoula Chatzimpinou, Anne Diehl, A. Tobias Harhoff, Kristina Driller, Bieke Vanslembrouck, Jian-Hua Chen, Kristaps Kairišs, Valentina Loconte, Mark A. Le Gros, Carolyn Larabell, Kürşad Turgay, Hartmut Oschkinat, Venera Weinhardt
Pubblicato in: npj Biofilms and Microbiomes, Numero 11, 2025, ISSN 2055-5008
Editore: Springer Science and Business Media LLC
DOI: 10.1038/s41522-025-00659-0

Compact Cell Imaging Device (CoCID) to provide insights into the cellular origins of viral infections

Autori: Kenneth Fahy, Venera Weinhardt, Maija Vihinen-Ranta, Nicola Fletcher, Dunja Skoko, Eva Pereiro, Pablo Gastaminza, Ralf Bartenschlager, Dimitri Scholz, Axel Ekman, Tony McEnroe
Pubblicato in: Journal of Physics: Photonics, 2021, ISSN 2515-7647
Editore: IOP Publishing
DOI: 10.1088/2515-7647/abfc5a

Progression of herpesvirus infection is inhibited by calcium reporter.

Autori: Kunnas, Kari; Vihinen-Ranta, Maija; Leclerc, Simon
Pubblicato in: microPublication biology, Numero 11, 2024, ISSN 2578-9430
Editore: Caltech Library

A protocol for full-rotation soft X-ray tomography of single cells

Autori: Jian-Hua Chen, Bieke Vanslembrouck, Valentina Loconte, Axel Ekman, Mirko Cortese, Ralf Bartenschlager, Gerry McDermott, Carolyn A. Larabell, Mark A. Le Gros, and Venera Weinhardt
Pubblicato in: STAR Protocols, 2022, ISSN 2666-1667
Editore: Elsevier
DOI: 10.1016/j.xpro.2022.101176

Functional roles of the membrane-associated AAV protein MAAP

Autori: Lionel Galibert, Amira Hyvönen, Reetta A. E. Eriksson, Salla Mattola, Vesa Aho, Sami Salminen, Justin D. Albers, Sanna K. Peltola, Saija Weman, Tiina Nieminen, Seppo Ylä-Herttuala, Hanna P. Lesch, Maija Vihinen-Ranta, Kari J. Airenne
Pubblicato in: Scientific Reports, Numero 11, 2023, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-021-01220-7

Parvovirus nonstructural protein 2 interacts with chromatin-regulating cellular proteins

Autori: Salla Mattola, Kari Salokas, Vesa Aho, Elina Mäntylä, Sami Salminen, Satu Hakanen, Einari A. Niskanen, Julija Svirskaite, Teemu O. Ihalainen, Kari J. Airenne, Minna Kaikkonen-Määttä, Colin R. Parrish, Markku Varjosalo, Maija Vihinen-Ranta
Pubblicato in: PLOS Pathogens, Numero 18, 2022, Pagina/e e1010353, ISSN 1553-7374
Editore: Public Library of Science (PLoS)
DOI: 10.1371/journal.ppat.1010353

<scp>CELS</scp>‐<scp>3D</scp>—Cutting edge light source for exciting fluorescence in microtome‐based <scp>3D</scp> microscopy and targeted correlative microscopy

Autori: Vladyslav Denyshchenko, Christopher Evans, Tiina O'Neill, Jakub Krstev, Emilia Filipczak, Silas O'Toole, Vladimir Lobaskin, Dimitri Scholz
Pubblicato in: Journal of Anatomy, Numero 246, 2025, Pagina/e 782-797, ISSN 0021-8782
Editore: Blackwell Publishing Inc.
DOI: 10.1111/joa.14164

Enhanced hepatitis E virus infection of polarised hepatocytes in vitro

Autori: Hannah M. Brown, Julien Marlet, Nancy León-Janampa, Denys Brand, Nicola F. Fletcher
Pubblicato in: Scientific Reports, Numero 15, 2025, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-025-92164-9

Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells.

Autori: Valentina Loconte; Valentina Loconte; Jian-Hua Chen; Jian-Hua Chen; Mirko Cortese; Axel Ekman; Mark A. Le Gros; Mark A. Le Gros; Carolyn A. Larabell; Carolyn A. Larabell; Ralf Bartenschlager; Ralf Bartenschlager; Venera Weinhardt; Venera Weinhardt
Pubblicato in: Cell Reports Methods, Numero 2, 2021, ISSN 2667-2375
Editore: Cell Press
DOI: 10.1016/j.crmeth.2021.100117

Automated 3D cytoplasm segmentation in soft X-ray tomography

Autori: Ayse Erozan, Philipp D. Lösel, Vincent Heuveline, Venera Weinhardt
Pubblicato in: iScience, Numero 27, 2025, Pagina/e 109856, ISSN 2589-0042
Editore: Elsevier BV
DOI: 10.1016/j.isci.2024.109856

Dehydration as alternative sample preparation for soft X‐ray tomography

Autori: Anthoula Chatzimpinou, Charlotta Funaya, David Rogers, Stephen O'Connor, Sergey Kapishnikov, Paul Sheridan, Kenneth Fahy, Venera Weinhardt
Pubblicato in: Journal of Microscopy, Numero 291, 2023, Pagina/e 248-255, ISSN 0022-2720
Editore: Blackwell Publishing Inc.
DOI: 10.1111/jmi.13214

Nuclear entry and egress of parvoviruses

Autori: Salla Mattola, Vesa Aho, Luisa F. Bustamante‐Jaramillo, Edoardo Pizzioli, Michael Kann, Maija Vihinen‐Ranta
Pubblicato in: Molecular Microbiology, Numero 118, 2023, Pagina/e 295-308, ISSN 0950-382X
Editore: Blackwell Publishing Inc.
DOI: 10.1111/mmi.14974

Extending Imaging Volume in Soft X‐Ray Tomography

Autori: Axel Ekman, Jian-Hua Chen, Bieke Vanslembrouck, Valentina Loconte, Carolyn A. Larabell, Mark A. Le Gros, Venera Weinhardt
Pubblicato in: Advanced Photonics Research, Numero 4, 2024, ISSN 2699-9293
Editore: Wiley
DOI: 10.1002/adpr.202200142

Imaging of Virus-Infected Cells with Soft X-ray Tomography

Autori: Damià Garriga, Francisco Javier Chichón, Bárbara M. Calisto, Diego S. Ferrero, Pablo Gastaminza, Eva Pereiro and Ana Joaquina Pérez-Berna
Pubblicato in: Viruses, Numero Monthly, 2021, ISSN 1999-4915
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v13112109

Persistence of Human Bocavirus 1 in Tonsillar Germinal Centers and Antibody-Dependent Enhancement of Infection

Autori: Man Xu, Maria Fernanda Perdomo, Salla Mattola, Lari Pyöriä, Mari Toppinen, Jianming Qiu, Maija Vihinen-Ranta, Klaus Hedman, Johanna Nokso-Koivisto, Leena-Maija Aaltonen, Maria Söderlund-Venermo
Pubblicato in: mBio, Numero 12, 2023, ISSN 2161-2129
Editore: American Society for Microbiology
DOI: 10.1128/mbio.03132-20

Mitochondrial Reorganization in Herpesvirus-Infected Cells

Autori: S Leclerc, K Kunnas, A Ekman, E Pereiro, K Fahy, C Larabell, V Aho, V Weinhardt, M Vihinen-Ranta
Pubblicato in: Microscopy and Microanalysis, Numero 29, 2023, Pagina/e 1153-1154, ISSN 1431-9276
Editore: Cambridge University Press
DOI: 10.1093/micmic/ozad067.589

Rapid hepatitis C virus replication machinery removal after antiviral treatment with DAA monitored by multimodal imaging

Autori: Victoria Castro, Gema Calvo, Ana J. Pérez-Berná, Kevin Mamprin, Sergey Kapishnikov, David Rogers, Stephen O'Connor, Paul Sheridan, Kenneth Fahy, Eva Pereiro, Pablo Gastaminza
Pubblicato in: Structure, 2025, ISSN 0969-2126
Editore: Cell Press
DOI: 10.1016/j.str.2025.05.015

Progression of herpesvirus infection remodels mitochondrial organization and metabolism

Autori: Simon Leclerc, Alka Gupta, Visa Ruokolainen, Jian-Hua Chen, Kari Kunnas, Axel A. Ekman, Henri Niskanen, Ilya Belevich, Helena Vihinen, Paula Turkki, Ana J. Perez-Berna, Sergey Kapishnikov, Elina Mäntylä, Maria Harkiolaki, Eric Dufour, Vesa Hytönen, Eva Pereiro, Tony McEnroe, Kenneth Fahy, Minna U. Kaikkonen, Eija Jokitalo, Carolyn A. Larabell, Venera Weinhardt, Salla Mattola, Vesa Aho, Maija Vi
Pubblicato in: PLOS Pathogens, Numero 20, 2024, Pagina/e e1011829, ISSN 1553-7374
Editore: Public Library of Science (PLoS)
DOI: 10.1371/journal.ppat.1011829

G2/M checkpoint regulation and apoptosis facilitate the nuclear egress of parvoviral capsids

Autori: Salla Mattola, Elina Mäntylä, Vesa Aho, Sami Salminen, Simon Leclerc, Mikko Oittinen, Kari Salokas, Jani Järvensivu, Satu Hakanen, Teemu O Ihalainen, Keijo Viiri, Maija Vihinen-Ranta
Pubblicato in: Frontiers in Cell and Developmental Biology, Numero 10, 2022, ISSN 2296-634X
Editore: Frontiers Media SA
DOI: 10.3389/fcell.2022.1070599

Concepts to Reveal Parvovirus–Nucleus Interactions

Autori: Salla Mattola, Satu Hakanen, Sami Salminen, Vesa Aho, Elina Mäntylä, Teemu O. Ihalainen, Michael Kann, Maija Vihinen-Ranta
Pubblicato in: Viruses, Numero 13, 2025, Pagina/e 1306, ISSN 1999-4915
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v13071306

Other (2)

Alternative cell entry mechanisms for SARS-CoV-2 and multiple animal viruses

Autori: Ravi Ojha, Anmin Jiang, Elina Mäntylä, Naphak Modhira, Robert Witte, Arnaud Gaudin, Lisa De Zanetti, Rachel Gormal, Maija Vihinen-Ranta, Jason Mercer, Maarit Suomalainen, Urs F. Greber, Yohei Yamauchi, Pierre Yves-Lozach, Ari Helenius, Olli Vapalahti, Paul Young, Daniel Watterson, Frédéric A. Meunier, Merja Joensuu, Giuseppe Balistreri
Pubblicato in: bioRxiv, 2023
Editore: Cold Spring Harbor Laboratory
DOI: 10.1101/2023.07.02.547368

Demonstrating Soft X-Ray Tomography in the lab for correlative cryogenic biological imaging using X-rays and light microscopy

Autori: Stephen O’Connor, David Rogers, Maryna Kobylynska, James Geraets, Katja Thaysen, Jacob Marcus Egebjerg, Madeleen C. Brink, Louisa Herbsleb, Michaela Salakova, Leon Fuchs, Frauke Alves, Claus Feldmann, Axel Ekman, Paul Sheridan, William Fyans, Tony McEnroe, Fergal O’Reily, Kenneth Fahy, Roland A. Fleck, Daniel Wüstner, Jeremy C. Simpson, Andreas Walter, Sergey Kapishnikov
Pubblicato in: bioRxiv, 2025
Editore: Cold Spring Harbor Laboratory
DOI: 10.1101/2024.12.23.629889

Book chapters (1)

Laboratory based correlative cryo-soft X-ray tomography and cryo-fluorescence microscopy

Autori: Kenneth Fahy, Sergey Kapishnikov, Martina Donnellan, Tony McEnroe, Fergal O'Reilly, William Fyans, Paul Sheridan
Pubblicato in: Methods in Cell Biology, Correlative Light and Electron Microscopy V, 2024, Pagina/e 293-320
Editore: Elsevier
DOI: 10.1016/bs.mcb.2024.02.033