Objective
PULSE: Polyphosphate Utilization in Living Systems of Mammals and their Effects. Here, we propose to uncover how mammalian systems utilize polyphosphate (polyP) and what roles it may play in health. First, we introduce a novel approach to understand how polyP levels affect cellular activity. We propose measuring metabolic activity, transcriptional differences, and morphological conditions induced by manipulating the abundance of polyP in specific subcellular compartments. To do this, we have developed a system to express either polyphosphate kinase (PPK) or exopolyphosphatases (PPX) in specific compartments to increase or decrease the polyP levels, respectively.
We further propose to interrogate the role polyP plays in liver health and acute liver damage caused by acetaminophen (APAP) toxicity. We will use a novel mouse model with ectopic expression of PPX to decrease the level of polyP present in the liver to determine the effect of polyP abundance on liver tissue response to acute APAP injury. We will analyze the pathology of the tissue through multiple methods including serum biomarkers, histopathology, immunohistochemistry, intravital multi-photon imaging, and pulsed-infrared laser-based tissue proteomics. In addition, using human patient samples from the UKE facility, we can analyze polyP levels in human tissue based on human-specific pathologies, allowing us to expand our study to include patient-relevant data.
At the conclusion of this project, we will substantially expand the baseline understanding of how mammalian systems utilize polyP. With the work from our mouse model, we will have a clearer understanding of what role polyP plays in the health of mammals. The additional data we will generate from human patient samples will allow us direct understanding into the role of polyP in human health as well.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences basic medicine pathology
- natural sciences biological sciences zoology mammalogy
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2025-PF
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
20251 Hamburg
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.