Objective
Rainbow trout are one of the most valuable fish species farmed in the European Union, thus knowledge of its immune system is of practical importance. This project is focused on the study of interleukin 2 (IL2), a central cytokine in the regulation of T-cell responses, which stimulates proliferation of these cells and increases cytotoxicity of CD8+ T cells, natural killer, B cells, monocyte/macrophages and neutrophils. IL2 is an important adjuvant for inactivated or subunit viral vaccines in humans and may have a potential role in protection against bacterial and viral diseases. Initially expression studies of IL2 in head-kidney and spleen will be examined by PCR, after administration of different immunostimulants, since this molecule is not constitutively expressed. Next, the activity of the recombinant protein (rIL2) will be established. An antibody to IL2 will also be generated for subsequent use in ELISPOT assays. Thus the specific cell-mediated immune response in trout will be quantified by enumerating the number of cells secreting IL2 in response to antigen, establishing a relationship between the size of the IL2 response and protection post-vaccination using a model bacterial, Aeromonas salmonicida, and viral, haermorrhagic septicaemia virus, VHSV, pathogen. Further experiments will be carried out using the rIL2 to increase the effectiveness of fish vaccines by inclusion in the vaccine. Following vaccination, the A. salmonicida and VHSV antibody titres will be determined for the serum and correlations sought between the size of the antibody response, the IL2 secreting cell response and resistance. Finally, IL2 secreting cells will be induced and identified using the monoclonal antibodies to detect intracellular IL2 and sorted by FACS. The sorted cells will be characterized in terms of their transcriptome, by microarray analysis. The arrays will also be used with RNA from cells stimulated with rIL2 to get an overview of genes affected in target cells.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology virology
- medical and health sciences basic medicine immunology
- medical and health sciences basic medicine pharmacology and pharmacy pharmaceutical drugs vaccines
- natural sciences biological sciences zoology ichthyology
- natural sciences biological sciences genetics RNA
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2007-2-1-IEF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
AB24 3FX Aberdeen
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.