Objective
The main objective of the project is to apply and further develop a multiplex DNA detection system for identification of unauthorized genetically modified organisms (GMOs) in European food and feed chains. The relevance of this is underscored by recent incidents with unapproved Bt10 maize, Bt73 rice and LL62 rice, which showed that lab-testing is unavoidable for maintenance of national and EU laws. This requires a reliable, cost-effective detection method. Real time PCR meets sensitivity demands up to a 0.1% level. However, detection and identification of GMOs in food is a time-consuming and expensive puzzle in many cases, when many subsequent real time PCR reactions have to be performed for identification. Padlockprobe, ligation-based multiplex detection does provide a method that can potentially meet all the demands of unauthorized GM detection. It is a three step reaction sequence starting with the detection of all possible targets in a single ligation reaction. Subsequent steps involve amplification by PCR and identification by microarray hybridization. The project has four distinct phases: 1) Identification of the DNA target sequences for padlock probe design. The combination of all available sequence information will be used for the building of a model for unauthorized GMO detection. This model will identify the most informative elements as well as any hiatus in sequence information; 2) Design of padlock probes for the DNA targets. This requires examination of secondary structures compromising the efficiency of the probes, and similarity to known DNA sequences resulting in non-specific reactions. 3) Testing of the padlockprobes in the lab. The most important parameters will be sensitivity, the relation between the sensitivity and the number of targets, and the specificity; 4) Validation of the developed method. Dissemination will occur throughout the project through presentations at (inter)national symposia and papers in peer-reviewed scientific journals.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2007-2-1-IEF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
6708 PB Wageningen
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.