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Content archived on 2024-05-29

VALIDATION OF GENES POTENTIALLY RESPONSIBLE FOR THE TOLERANCE AND HEAVY METAL HYPERACCUMULATION ABILITY IN HIGHER PLANTS USING QTL MAPPING AND THE TOOLS OF MOLECULAR EVOLUTION

Objective

A rare class of plants named hyperaccumulators can tolerate heavy metals and also accumulate such metals to extremely high concentrations in their shoots. Classical techniques of screening have given clues to isolated genes involved in metal tolerance and homeostasis. However, their direct relationship with metal adaptation has not yet been established and the major genes of tolerance remain to be identified. Within the Arabidopsis genus, A. halleri has been shown to tolerate and hyperaccumulate Zn constitu tively while other species do not tolerate Zn (A. thaliana, A. lyrata). Interspecific crosses between A. halleri and A. lyrata, led to the mapping of three QTL regions for Zn tolerance. Within A. halleri species, a wide range of quantitative variation for tolerance has been measured among populations covering the natural area of distribution of the species. Genotypes that showed high and low tolerance were isolated from metallicolous and non-metallicolous populations respectively. We propose to use this exc eptional biological material to map candidate genes, to identify which of them are localised in QTL regions of tolerance, and to test, through the analysis of sequence variation among alleles, which genes are involved in the metal-tolerance adaptation at t he inter- and intra-specific level. This multidisciplinary approach integrates results obtained at the molecular, functional and ecological levels and will offer new perspectives on the study of the molecular and evolutionary processes responsible for meta l tolerance. This project will contribute to a better understanding of metal tolerance, hyperaccummulation and homeostasis. We believe that this action will help to increase the level of excellence of European scientists and also aid in the development of an EU Research Unit with an internationally recognized expertise in molecular and evolutionary aspects of heavy metal tolerance in plants.

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Keywords

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Topic(s)

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Call for proposal

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FP6-2004-MOBILITY-5
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Funding Scheme

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EIF - Marie Curie actions-Intra-European Fellowships

Coordinator

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
EU contribution
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Total cost

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