Infrastructure for NMR, EM and X-rays for translational research

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Second report on the interaction with industrial parties

Report on the interaction with industrial parties (EMBL-GR)

Delivery of 370 days of access as High-end Data Acquisition to SONNMRLSF at UU
Second report on outreach activities
Start of TA to HT integrated fragment screening in Grenoble

Start of TA to HT integrated fragment screening in Grenoble (EMBL-GR)

Secondary screening of a set of derivatives around an initial hit making use of the EU-OPENSCREEN library
Delivery of 145 days of access to NMR at CEITEC
Reports on feasibility studies on the promising translational targets
Setup for lower background for diffraction data on membrane proteins
Delivery of 100 days of access as Enhanced Support to SONNMRLSF at UU
At least two articles for the general public
Report on Coordination activities with Virtual Research Environments
Storage and queuing facilities at ESRF
120 samples through “Structural Audit”
Report on novel biophysical methods
Hiring a Project Manager
Protocols and tools for using ssNMR to study protein complexes associated with cellular compartments
Report on Coordination activities with PDBe and Elixir
Delivery of 120 samples through the Structural Audit procedure at NKI
Report on a model for future structural biology access in Europe
Delivery of 305 days of High-End Data Acquisition to NMR at BMRZ
TA access to HT crystallization and crystal mounting

TA access to HT crystallization and crystal mounting (EMBL-GR)

Delivery of 75 days of access to Cryo-EM at CSIC
First report on the interaction with industrial parties

Report on the interaction with industrial parties (EMBL-GR)

Report on financial sustainability
Delivery of 2389 hours of access to Beam MX at Diamond
Second report on conferences and meetings with iNEXT representation

Second report on conferences and meetings with iNEXT representation (EMBL-HH)

Report on the organization of TDC workshops
Access to NMR probe(s) optimized for the investigation of membrane proteins (binding studies/,screening)
Start of TA to integrated screening for focused libraries at EMBL-GR/ESRF
Workflow for EM-based determination of membrane protein structures

Workflow for EM-based determination of membrane protein structures (EMBL-HD)

Delivery of 78 days of full service ‘Protein to XTAL’at EMBL-HH
Data management system for ligand and fragment screening, extending ISPyB
Protocols for membrane protein structure determination by solid-state NMR
Protocols for correlative fluorescent labeling of proteins in vitro and in situ
Delivery of 130 days of access to cryo-EM at CEITEC
Second summary report on networking activities

Second summary report on networking activities (EMBL-HH)

Draft sustainability plan
Report on the attendance to EMBO and FEBS meetings

Report on the attendance to EMBO, FEBS and similar meetings

Automatic EM Image Processing workflow in the absence of structural flexibility
Automation of the on-line SEC setups at the partner SAXS beamlines
First summary report on networking activities

First summary report on networking activities (EMBL-HH)

Integrated submission system operational
Protocols and tools for protein interactions in mammalian cells by NMR
Delivery of 144 days of access to Titan KRIO cyro-EM at NeCEN
Delivery of 76 days of full service ‘Protein to SAXS at EMBL-HH
Delivery of 192 supervised access days to EMCF at EMBL-HD
Protocols for data acquisition for correlative CLEXM
Storage and queuing facilities at Diamond
Delivery of 5450 supervised access hours to ALMF at EMBL-HD
Report on comparative analysis of data models in all disciplines and strategy for integration
Delivery of 110 days of access to NMR at RALF-NMR
Delivery of 36 days of accessto Integrated fragment screening by MX at EMBL-GR
Report on the organization of annual user meetings
Delivery of 1555 hours of access to MX beamtime at EMBL-HH
Delivery of 14 days of access to Integrated compound screening (focused libraries) by MX at EMBL-GR
Protocols for RNA sample optimization and DNA/RNA delivery into bacterial and mammalian cells. for in-cell NMR studies
Access to an integrated miniaturized HT-SEC-SAXS set-up

Access to an integrated miniaturized HT-SEC-SAXS set-up (EMBL-HH)

Management Guidelines
Delivery of 70 days of access to Integrated Macromolecular Crystallography service at EMBL-GR
Optimized protocols for reconstitution of membrane proteins

Optimized protocols for reconstitution of membrane proteins (EMBL-HH)

Automated SAXS pipeline for membrane proteins, integrated into ISPyB

Automated SAXS pipeline for membrane proteins, integrated into ISPyB (EMBL-HH)

Automated pipelines for NMR-based structure determination accessible online
Delivery of 10 days of Structural Audit to NMR at BMRZ
Delivery of 2720 hours of access to Beam MX/SAXS at SOLEIL
Start of TA to HT integrated fragment screening at Diamond
Fully automatic screening, large scale X-ray data collection pipelines
Start of TA access to HT fragment screening by NMR
Delivery of 10 days of access as Structural Audit to SONNMRLSF at UU
Delivery of 100 days of Enhanced Support to NMR at BMRZ
Delivery of 212 days of access to NMR at FVB-FMP
Report on demands of the user community
Representation on RI bodies or Committees
Protocols for preparing ssNMR samples in bacterial and mammalian cell fractions and cells
15 structure validation projects through biophysics facility
Characterization of 120 “SAXS x-audit” samples at EMBL-HH
Delivery of 346 days of High-End Data Acquisition to NMR at CERM/CIRMMP
SONICC-based centring of membrane protein crystals
CRIMS support for ligand and fragment screening

CRIMS support for ligand and fragment screening (EMBL-GR)

Data management system for a EM workflows, extending ISPyB
Fragment library distributed to partners
Delivery of 775 hours of access to SAXS beamtime at EMBL-HH
Summary report on networking activities with industry

Summary report on networking activities with industry (EMBL-GR)

First report on outreach activities
First report on conferences and meetings with iNEXT representation

First report on conferences and meetings with iNEXT representation (EMBL-HH)

Delivery of access for 60 mail-in SAXS sessions at Diamond
Delivery of 30 samples analysed for Structural Audit at CSIC

Delivery of 30 samples analysed for Structural Audit at CSIC

Optimized workflows for ligand screening by NMR
Best practice workflows for cryo electron tomography specimen preparation
Fluorophore knock in cell lines for structural studies

Fluorophore knock in cell lines for structural studies (EMBL-HD)

Protocols for cooperation with ESFRI BMS RIs
5 integrated structure validation projects through combined access in biophysics and imaging
Delivery of access to 10 screening campaigns at Diamond
Delivery of 20 projects for biophysical characterisation at NKI
Delivery of 10 days of Structural Audit to NMR at CERM/CIRMMP
Delivery of 750 hours of access to the BioMAX beamline at MAXIV
Delivery of 100 days of Enhanced Support to NMR at CERM/CIRMMP
15 structure validation projects through advanced imaging facility
Delivery of 110 days of access to cryo-EM at Diamond

Publications

Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo

Author(s): Ajasja Ljubetič, Fabio Lapenta, Helena Gradišar, Igor Drobnak, Jana Aupič, Žiga Strmšek, Duško Lainšček, Iva Hafner-Bratkovič, Andreja Majerle, Nuša Krivec, Mojca Benčina, Tomaž Pisanski, Tanja Ćirković Veličković, Adam Round, José María Carazo, Roberto Melero, Roman Jerala
Published in: Nature Biotechnology, Issue 35(11), 2017, Page(s) 1094-1101, ISSN 1087-0156
Publisher: Nature Publishing Group
DOI: 10.1038/nbt.3994

Structural Basis for Polyproline-Mediated Ribosome Stalling and Rescue by the Translation Elongation Factor EF-P

Author(s): Paul Huter, Stefan Arenz, Lars V. Bock, Michael Graf, Jan Ole Frister, Andre Heuer, Lauri Peil, Agata L. Starosta, Ingo Wohlgemuth, Frank Peske, Jiří Nováček, Otto Berninghausen, Helmut Grubmüller, Tanel Tenson, Roland Beckmann, Marina V. Rodnina, Andrea C. Vaiana, Daniel N. Wilson
Published in: Molecular Cell, Issue 68/3, 2017, Page(s) 515-527.e6, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2017.10.014

Characterization of the Conjugation Pattern in Large Polysaccharide-Protein Conjugates by NMR Spectroscopy

Author(s): Stefano Giuntini, Evita Balducci, Linda Cerofolini, Enrico Ravera, Marco Fragai, Francesco Berti, Claudio Luchinat
Published in: Angewandte Chemie International Edition, Issue 56/47, 2017, Page(s) 14997-15001, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201709274

Structural Snapshots of α-1,3-Galactosyltransferase with Native Substrates: Insight into the Catalytic Mechanism of Retaining Glycosyltransferases

Author(s): David Albesa-Jové, M. Ángela Sainz-Polo, Alberto Marina, Marcelo E. Guerin
Published in: Angewandte Chemie International Edition, Issue 56/47, 2017, Page(s) 14853-14857, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201707922

Enhanced Pru p 3 IgE-binding activity by selective free fatty acid-interaction

Author(s): Pawel Dubiela, Roberta Aina, Dominika Polak, Sabine Geiselhart, Piotr Humeniuk, Barbara Bohle, Stefano Alessandri, Rebecca Del Conte, Francesca Cantini, Tomasz Borowski, Merima Bublin, Karin Hoffmann-Sommergruber
Published in: Journal of Allergy and Clinical Immunology, Issue 140/6, 2017, Page(s) 1728-1731.e10, ISSN 0091-6749
Publisher: Mosby Inc.
DOI: 10.1016/j.jaci.2017.06.016

Impact of Azidohomoalanine Incorporation on Protein Structure and Ligand Binding

Author(s): Florian Lehner, Denis Kudlinzki, Christian Richter, Henrike M. Müller-Werkmeister, Katharina B. Eberl, Jens Bredenbeck, Harald Schwalbe, Robert Silvers
Published in: ChemBioChem, Issue 18/23, 2017, Page(s) 2340-2350, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201700437

A molecular chaperone activity of CCS restores the maturation of SOD1 fALS mutants

Author(s): Enrico Luchinat, Letizia Barbieri, Lucia Banci
Published in: Scientific Reports, Issue 7/1, 2017, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-017-17815-y

Structural basis for genome wide recognition of 5-bp GC motifs by SMAD transcription factors

Author(s): Pau Martin-Malpartida, Marta Batet, Zuzanna Kaczmarska, Regina Freier, Tiago Gomes, Eric Aragón, Yilong Zou, Qiong Wang, Qiaoran Xi, Lidia Ruiz, Angela Vea, José A. Márquez, Joan Massagué, Maria J. Macias
Published in: Nature Communications, Issue 8/1, 2017, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-017-02054-6

Structure of outer membrane protein G in lipid bilayers

Author(s): Joren S. Retel, Andrew J. Nieuwkoop, Matthias Hiller, Victoria A. Higman, Emeline Barbet-Massin, Jan Stanek, Loren B. Andreas, W. Trent Franks, Barth-Jan van Rossum, Kutti R. Vinothkumar, Lieselotte Handel, Gregorio Giuseppe de Palma, Benjamin Bardiaux, Guido Pintacuda, Lyndon Emsley, Werner Kühlbrandt, Hartmut Oschkinat
Published in: Nature Communications, Issue 8/1, 2017, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-017-02228-2

Structural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase

Author(s): Sina Reckel, Charlotte Gehin, Delphine Tardivon, Sandrine Georgeon, Tim Kükenshöner, Frank Löhr, Akiko Koide, Lena Buchner, Alejandro Panjkovich, Aline Reynaud, Sara Pinho, Barbara Gerig, Dmitri Svergun, Florence Pojer, Peter Güntert, Volker Dötsch, Shohei Koide, Anne-Claude Gavin, Oliver Hantschel
Published in: Nature Communications, Issue 8/1, 2017, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-017-02313-6

Atomic structural details of a protein grafted onto gold nanoparticles

Author(s): Stefano Giuntini, Linda Cerofolini, Enrico Ravera, Marco Fragai, Claudio Luchinat
Published in: Scientific Reports, Issue 7/1, 2017, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-017-18109-z

Molecular mechanism for the subversion of the retromer coat by the Legionella effector RidL

Author(s): Miguel Romano-Moreno, Adriana L. Rojas, Chad D. Williamson, David C. Gershlick, María Lucas, Michail N. Isupov, Juan S. Bonifacino, Matthias P. Machner, Aitor Hierro
Published in: Proceedings of the National Academy of Sciences, Issue 114/52, 2017, Page(s) E11151-E11160, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1715361115

Long-range paramagnetic NMR data can provide a closer look on metal coordination in metalloproteins

Author(s): Linda Cerofolini, Tommaso Staderini, Stefano Giuntini, Enrico Ravera, Marco Fragai, Giacomo Parigi, Roberta Pierattelli, Claudio Luchinat
Published in: JBIC Journal of Biological Inorganic Chemistry, Issue 23/1, 2018, Page(s) 71-80, ISSN 0949-8257
Publisher: Springer Verlag
DOI: 10.1007/s00775-017-1511-y

Intracellular metal binding and redox behavior of human DJ-1

Author(s): Letizia Barbieri, Enrico Luchinat, Lucia Banci
Published in: JBIC Journal of Biological Inorganic Chemistry, Issue 23/1, 2018, Page(s) 61-69, ISSN 0949-8257
Publisher: Springer Verlag
DOI: 10.1007/s00775-017-1509-5

Dynamic control of RSK complexes by phosphoswitch-based regulation

Author(s): Gergő Gógl, Beáta Biri-Kovács, Ádám L. Póti, Henrietta Vadászi, Bálint Szeder, Andrea Bodor, Gitta Schlosser, András Ács, Lilla Turiák, László Buday, Anita Alexa, László Nyitray, Attila Reményi
Published in: The FEBS Journal, Issue 285/1, 2018, Page(s) 46-71, ISSN 1742-464X
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/febs.14311

Combined 1H-Detected Solid-State NMR Spectroscopy and Electron Cryotomography to Study Membrane Proteins across Resolutions in Native Environments

Author(s): Baker LA, Sinnige T, Schellenberger P, de Keyzer J, Siebert CA, Driessen AJM, Baldus M, Grünewald K
Published in: Structure, Issue 26/1, 2018, Page(s) 161-170.e3, ISSN 0969-2126
Publisher: Elsevier

Structural basis of RNA polymerase III transcription initiation

Author(s): Guillermo Abascal-Palacios, Ewan Phillip Ramsay, Fabienne Beuron, Edward Morris, Alessandro Vannini
Published in: Nature, Issue 553/7688, 2018, Page(s) 301-306, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/nature25441

1 H NMR Spectroscopy of [FeFe] Hydrogenase: Insight into the Electronic Structure of the Active Site

Author(s): Sigrun Rumpel, Enrico Ravera, Constanze Sommer, Edward Reijerse, Christophe Farès, Claudio Luchinat, Wolfgang Lubitz
Published in: Journal of the American Chemical Society, Issue 140/1, 2017, Page(s) 131-134, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.7b11196

Structural plasticity of the N-terminal capping helix of the TPR domain of kinesin light chain

Author(s): The Quyen Nguyen, Mélanie Chenon, Fernando Vilela, Christophe Velours, Magali Aumont-Nicaise, Jessica Andreani, Paloma F. Varela, Paola Llinas, Julie Ménétrey
Published in: PLOS ONE, Issue October 16, 2017, 2017, Page(s) 1-20, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0186354.s004

Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research

Author(s): Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
Published in: Structure, Issue 26, 2018, Page(s) 345-355, ISSN 0969-2126
Publisher: Cell Press
DOI: 10.1016/j.str.2018.01.007

CHROMIXS: automatic and interactive analysis of chromatography-coupled small-angle X-ray scattering data

Author(s): Alejandro Panjkovich, Dmitri I Svergun
Published in: Bioinformatics, Issue 28 December 2017, 2017, Page(s) 1-3, ISSN 1367-4803
Publisher: Oxford University Press
DOI: 10.1093/bioinformatics/btx846

Automated harvesting and processing of protein crystals through laser photoablation

Author(s): Ulrich Zander, Guillaume Hoffmann, Irina Cornaciu, Jean-Pierre Marquette, Gergely Papp, Christophe Landret, Gaël Seroul, Jérémy Sinoir, Martin Röwer, Frank Felisaz, Sonia Rodriguez-Puente, Vincent Mariaule, Peter Murphy, Magali Mathieu, Florent Cipriani, José Antonio Márquez
Published in: Acta Crystallographica Section D Structural Biology, Issue 72/4, 2016, Page(s) 454-466, ISSN 2059-7983
Publisher: John Wiley & Sons Ltd
DOI: 10.1107/S2059798316000954

RavN is a member of a previously unrecognized group of Legionella pneumophila E3 ubiquitin ligases

Author(s): Yi-Han Lin, María Lucas, Timothy R. Evans, Guillermo Abascal-Palacios, Alexandra G. Doms, Nicole A. Beauchene, Adriana L. Rojas, Aitor Hierro, Matthias P. Machner
Published in: PLOS Pathogens, Issue 14/2, 2018, Page(s) e1006897, ISSN 1553-7374
Publisher: PLOS
DOI: 10.1371/journal.ppat.1006897

An allosteric binding site of the α7 nicotinic acetylcholine receptor revealed in a humanized acetylcholine-binding protein

Author(s): Florian Delbart, Marijke Brams, Fabian Gruss, Sam Noppen, Steve Peigneur, Sandro Boland, Patrick Chaltin, Jose Brandao-Neto, Frank von Delft, Wouter G. Touw, Robbie P. Joosten, Sandra Liekens, Jan Tytgat, Chris Ulens
Published in: Journal of Biological Chemistry, Issue 293/7, 2018, Page(s) 2534-2545, ISSN 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.M117.815316

Evaluation of the Stability of DNA i-Motifs in the Nuclei of Living Mammalian Cells

Author(s): Simon Dzatko, Michaela Krafcikova, Robert Hänsel-Hertsch, Tomas Fessl, Radovan Fiala, Tomas Loja, Daniel Krafcik, Jean-Louis Mergny, Silvie Foldynova-Trantirkova, Lukas Trantirek
Published in: Angewandte Chemie International Edition, Issue 57/8, 2018, Page(s) 2165-2169, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201712284

A family of unconventional deubiquitinases with modular chain specificity determinants

Author(s): Thomas Hermanns, Christian Pichlo, Ilka Woiwode, Karsten Klopffleisch, Katharina F. Witting, Huib Ovaa, Ulrich Baumann, Kay Hofmann
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-03148-5

Multi-channel in situ dynamic light scattering instrumentation enhancing biological small-angle X-ray scattering experiments at the PETRA III beamline P12

Author(s): Sven Falke, Karsten Dierks, Clement Blanchet, Melissa Graewert, Florent Cipriani, Rob Meijers, Dmitri Svergun, Christian Betzel
Published in: Journal of Synchrotron Radiation, Issue 25/2, 2018, Page(s) 361-372, ISSN 1600-5775
Publisher: IUCr
DOI: 10.1107/S1600577517017568

Homology-based hydrogen bond information improves crystallographic structures in the PDB

Author(s): Bart van Beusekom, Wouter G. Touw, Mahidhar Tatineni, Sandeep Somani, Gunaretnam Rajagopal, Jinquan Luo, Gary L. Gilliland, Anastassis Perrakis, Robbie P. Joosten
Published in: Protein Science, Issue 27/3, 2018, Page(s) 798-808, ISSN 0961-8368
Publisher: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.3353

Solution NMR structure of yeast Rcf1, a protein involved in respiratory supercomplex formation

Author(s): Shu Zhou, Pontus Pettersson, Jingjing Huang, Johannes Sjöholm, Dan Sjöstrand, Régis Pomès, Martin Högbom, Peter Brzezinski, Lena Mäler, Pia Ädelroth
Published in: Proceedings of the National Academy of Sciences, Issue 115/12, 2018, Page(s) 3048-3053, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1712061115

Multispecific Substrate Recognition in a Proton-Dependent Oligopeptide Transporter.

Author(s): Martinez Molledo M, Quistgaard EM, Flayhan A, Pieprzyk J, Löw C
Published in: Structure, Issue Mar 6;26(3), 2018, Page(s) 467-476, ISSN 0969-2126
Publisher: Elsevier

A multidisciplinary study of 3-(β- d -glucopyranosyl)-5-substituted-1,2,4-triazole derivatives as glycogen phosphorylase inhibitors: Computation, synthesis, crystallography and kinetics reveal new potent inhibitors

Author(s): Sándor Kun, Jaida Begum, Efthimios Kyriakis, Evgenia C.V. Stamati, Thomas A. Barkas, Eszter Szennyes, Éva Bokor, Katalin E. Szabó, George A. Stravodimos, Ádám Sipos, Tibor Docsa, Pál Gergely, Colin Moffatt, Myrto S. Patraskaki, Maria C. Kokolaki, Alkistis Gkerdi, Vassiliki T. Skamnaki, Demetres D. Leonidas, László Somsák, Joseph M. Hayes
Published in: European Journal of Medicinal Chemistry, Issue 147, 2018, Page(s) 266-278, ISSN 0223-5234
Publisher: Elsevier BV
DOI: 10.1016/j.ejmech.2018.01.095

Structural model of human dUTPase in complex with a novel proteinaceous inhibitor

Author(s): Kinga Nyíri, Haydyn D. T. Mertens, Borbála Tihanyi, Gergely N. Nagy, Bianka Kőhegyi, Judit Matejka, Matthew J. Harris, Judit E. Szabó, Veronika Papp-Kádár, Veronika Németh-Pongrácz, Olivér Ozohanics, Károly Vékey, Dmitri I. Svergun, Antoni J. Borysik, Beáta G. Vértessy
Published in: Scientific Reports, Issue 8/1, 2018, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-018-22145-8

Structures of Teneurin adhesion receptors reveal an ancient fold for cell-cell interaction

Author(s): Verity A. Jackson, Dimphna H. Meijer, Maria Carrasquero, Laura S. van Bezouwen, Edward D. Lowe, Colin Kleanthous, Bert J. C. Janssen, Elena Seiradake
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-03460-0

Structural changes of TasA in biofilm formation of Bacillus subtilis

Author(s): Anne Diehl, Yvette Roske, Linda Ball, Anup Chowdhury, Matthias Hiller, Noel Molière, Regina Kramer, Daniel Stöppler, Catherine L. Worth, Brigitte Schlegel, Martina Leidert, Nils Cremer, Natalja Erdmann, Daniel Lopez, Heike Stephanowitz, Eberhard Krause, Barth-Jan van Rossum, Peter Schmieder, Udo Heinemann, Kürşad Turgay, Ümit Akbey, Hartmut Oschkinat
Published in: Proceedings of the National Academy of Sciences, Issue 115/13, 2018, Page(s) 3237-3242, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1718102115

1H, 13C, and 15N chemical shift assignments of a G-quadruplex forming sequence within the KRAS proto-oncogene promoter region

Author(s): Julien Marquevielle, M. V. Vasantha Kumar, Jean-Louis Mergny, Gilmar F. Salgado
Published in: Biomolecular NMR Assignments, Issue 12/1, 2018, Page(s) 123-127, ISSN 1874-2718
Publisher: Springer Verlag
DOI: 10.1007/s12104-017-9793-0

A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein–RNA Interactions

Author(s): Po-chia Chen, Pawel Masiewicz, Vladimir Rybin, Dmitri Svergun, Janosch Hennig
Published in: ACS Combinatorial Science, Issue 20/4, 2018, Page(s) 197-202, ISSN 2156-8952
Publisher: American Chemical Society
DOI: 10.1021/acscombsci.8b00007

Structural characterization of the intrinsically disordered domain of Mycobacterium tuberculosis protein tyrosine kinase A

Author(s): Anna Niesteruk, Marie Hutchison, Sridhar Sreeramulu, Hendrik R.A. Jonker, Christian Richter, Rupert Abele, Christoph Bock, Harald Schwalbe
Published in: FEBS Letters, Issue 592/7, 2018, Page(s) 1233-1245, ISSN 0014-5793
Publisher: Elsevier BV
DOI: 10.1002/1873-3468.13022

RPAP3 provides a flexible scaffold for coupling HSP90 to the human R2TP co-chaperone complex

Author(s): Fabrizio Martino, Mohinder Pal, Hugo Muñoz-Hernández, Carlos F. Rodríguez, Rafael Núñez-Ramírez, David Gil-Carton, Gianluca Degliesposti, J. Mark Skehel, S. Mark Roe, Chrisostomos Prodromou, Laurence H. Pearl, Oscar Llorca
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-03942-1

The cysteine-reactive small molecule ebselen facilitates effective SOD1 maturation

Author(s): Michael J. Capper, Gareth S. A. Wright, Letizia Barbieri, Enrico Luchinat, Eleonora Mercatelli, Luke McAlary, Justin J. Yerbury, Paul M. O’Neill, Svetlana V. Antonyuk, Lucia Banci, S. Samar Hasnain
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-04114-x

Functional interaction of low-homology FRPs from different cyanobacteria with Synechocystis OCP

Author(s): Yury B. Slonimskiy, Eugene G. Maksimov, Evgeny P. Lukashev, Marcus Moldenhauer, Cy M. Jeffries, Dmitri I. Svergun, Thomas Friedrich, Nikolai N. Sluchanko
Published in: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Issue 1859/5, 2018, Page(s) 382-393, ISSN 0005-2728
Publisher: Elsevier BV
DOI: 10.1016/j.bbabio.2018.03.001

Integrated beamline control and data acquisition for small-angle X-ray scattering at the P12 BioSAXS beamline at PETRAIII storage ring DESY

Author(s): Nelly R. Hajizadeh, Daniel Franke, Dmitri I. Svergun
Published in: Journal of Synchrotron Radiation, Issue 25/3, 2018, Page(s) 906-914, ISSN 1600-5775
Publisher: IUCr
DOI: 10.1107/S1600577518005398

Consensus Bayesian assessment of protein molecular mass from solution X-ray scattering data

Author(s): Nelly R. Hajizadeh, Daniel Franke, Cy M. Jeffries, Dmitri I. Svergun
Published in: Scientific Reports, Issue 8/1, 2018, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-018-25355-2

Structure of the hexagonal surface layer on Caulobacter crescentus cells

Author(s): Tanmay A. M. Bharat, Danguole Kureisaite-Ciziene, Gail G. Hardy, Ellen W. Yu, Jessica M. Devant, Wim J. H. Hagen, Yves V. Brun, John A. G. Briggs, Jan Löwe
Published in: Nature Microbiology, Issue 2, 2017, Page(s) 17059, ISSN 2058-5276
Publisher: Springer Nature
DOI: 10.1038/nmicrobiol.2017.59

The structure of FIV reverse transcriptase and its implications for non-nucleoside inhibitor resistance

Author(s): Meytal Galilee, Akram Alian
Published in: PLOS Pathogens, Issue 14/1, 2018, Page(s) e1006849, ISSN 1553-7374
Publisher: PLOS
DOI: 10.1371/journal.ppat.1006849

NMR-Based Plasma Metabolomics at Set Intervals in Newborn Dairy Calves with Severe Sepsis

Author(s): Abdullah Basoglu, Ismail Sen, Gaia Meoni, Leonardo Tenori, Amir Naseri
Published in: Mediators of Inflammation, Issue 2018, 2018, Page(s) 1-12, ISSN 0962-9351
Publisher: Hindawi Publishing Corporation
DOI: 10.1155/2018/8016510

iNEXT: a European facility network to stimulate translational structural biology

Author(s): iNEXT Consortium
Published in: FEBS Letters, 2018, ISSN 0014-5793
Publisher: Elsevier BV
DOI: 10.1002/1873-3468.13062

Complete assignment of Ala, Ile, Leu, Met and Val methyl groups of human blood group A and B glycosyltransferases using lanthanide-induced pseudocontact shifts and methyl–methyl NOESY

Author(s): Friedemann Flügge, Thomas Peters
Published in: Journal of Biomolecular NMR, Issue 70/4, 2018, Page(s) 245-259, ISSN 0925-2738
Publisher: Kluwer Academic Publishers
DOI: 10.1007/s10858-018-0183-4

Engineering l -asparaginase for spontaneous formation of calcium phosphate bioinspired microreactors

Author(s): Alexandra Louka, Irina Matlahov, Stefano Giuntini, Linda Cerofolini, Andrea Cavallo, Serena Pillozzi, Enrico Ravera, Marco Fragai, Annarosa Arcangeli, Ayyalusamy Ramamoorthy, Gil Goobes, Claudio Luchinat
Published in: Physical Chemistry Chemical Physics, Issue 20/18, 2018, Page(s) 12719-12726, ISSN 1463-9076
Publisher: Royal Society of Chemistry
DOI: 10.1039/c8cp00419f

Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S

Author(s): Beatriz Trastoy, Erik Klontz, Jared Orwenyo, Alberto Marina, Lai-Xi Wang, Eric J. Sundberg, Marcelo E. Guerin
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-04300-x

Targeting RNA structure in SMN2 reverses spinal muscular atrophy molecular phenotypes

Author(s): Amparo Garcia-Lopez, Francesca Tessaro, Hendrik R. A. Jonker, Anna Wacker, Christian Richter, Arnaud Comte, Nikolaos Berntenis, Roland Schmucki, Klas Hatje, Olivier Petermann, Gianpaolo Chiriano, Remo Perozzo, Daniel Sciarra, Piotr Konieczny, Ignacio Faustino, Guy Fournet, Modesto Orozco, Ruben Artero, Friedrich Metzger, Martin Ebeling, Peter Goekjian, Benoît Joseph, Harald Schwalbe, Leonardo Sca
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-04110-1

Crystal structure of thebaine 6-O-demethylase from the morphine biosynthesis pathway

Author(s): Anna Kluza, Ewa Niedzialkowska, Katarzyna Kurpiewska, Zuzanna Wojdyla, Matthew Quesne, Ewa Kot, Przemyslaw J. Porebski, Tomasz Borowski
Published in: Journal of Structural Biology, Issue 202/3, 2018, Page(s) 229-235, ISSN 1047-8477
Publisher: Academic Press
DOI: 10.1016/j.jsb.2018.01.007

Enabling STD-NMR fragment screening using stabilized native GPCR: A case study of adenosine receptor

Author(s): Sébastien Igonet, Claire Raingeval, Erika Cecon, Maja Pučić-Baković, Gordan Lauc, Olivier Cala, Maciej Baranowski, Javier Perez, Ralf Jockers, Isabelle Krimm, Anass Jawhari
Published in: Scientific Reports, Issue 8/1, 2018, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-018-26113-0

Generation and validation of homozygous fluorescent knock-in cells using CRISPR–Cas9 genome editing

Author(s): Birgit Koch, Bianca Nijmeijer, Moritz Kueblbeck, Yin Cai, Nike Walther, Jan Ellenberg
Published in: Nature Protocols, Issue 13/6, 2018, Page(s) 1465-1487, ISSN 1754-2189
Publisher: Nature Publishing Group
DOI: 10.1038/nprot.2018.042

Quantitative mapping of fluorescently tagged cellular proteins using FCS-calibrated four-dimensional imaging

Author(s): Antonio Z Politi, Yin Cai, Nike Walther, M Julius Hossain, Birgit Koch, Malte Wachsmuth, Jan Ellenberg
Published in: Nature Protocols, Issue 13/6, 2018, Page(s) 1445-1464, ISSN 1754-2189
Publisher: Nature Publishing Group
DOI: 10.1038/nprot.2018.040

Insights into catalysis and function of phosphoribosyl-linked serine ubiquitination

Author(s): Sissy Kalayil, Sagar Bhogaraju, Florian Bonn, Donghyuk Shin, Yaobin Liu, Ninghai Gan, Jérôme Basquin, Paolo Grumati, Zhao-Qing Luo, Ivan Dikic
Published in: Nature, Issue 557/7707, 2018, Page(s) 734-738, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-018-0145-8

Correlative microscopy for structural microbiology

Author(s): Stuart C Howes, Roman I Koning, Abraham J Koster
Published in: Current Opinion in Microbiology, Issue 43, 2018, Page(s) 132-138, ISSN 1369-5274
Publisher: Elsevier BV
DOI: 10.1016/j.mib.2018.01.009

Advances in the cellular structural biology of nucleic acids

Author(s): Ilektra-Chara Giassa, Jan Rynes, Tomas Fessl, Silvie Foldynova-Trantirkova, Lukas Trantirek
Published in: FEBS Letters, Issue 592/12, 2018, Page(s) 1997-2011, ISSN 0014-5793
Publisher: Elsevier BV
DOI: 10.1002/1873-3468.13054

Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions

Author(s): Daniel Franke, Cy M. Jeffries, Dmitri I. Svergun
Published in: Biophysical Journal, Issue 114/11, 2018, Page(s) 2485-2492, ISSN 0006-3495
Publisher: Biophysical Society
DOI: 10.1016/j.bpj.2018.04.018

Biophysical and structural characterization of the putative nickel chaperone CooT from Carboxydothermus hydrogenoformans

Author(s): M. Alfano, J. Pérard, R. Miras, P. Catty, C. Cavazza
Published in: JBIC Journal of Biological Inorganic Chemistry, Issue 23/5, 2018, Page(s) 809-817, ISSN 0949-8257
Publisher: Springer Verlag
DOI: 10.1007/s00775-018-1576-2

Influence of pH on structure and stability of the sweet protein MNEI

Author(s): Roberta Spadaccini, Serena Leone, Michele Fortunato Rega, Christian Richter, Delia Picone
Published in: FEBS Letters, Issue Sept 29, 2016, 2016, ISSN 0014-5793
Publisher: Elsevier BV
DOI: 10.1002/1873-3468.12437

Cross-membranes orchestrate compartmentalization and morphogenesis in Streptomyces

Author(s): Katherine Celler, Roman I. Koning, Joost Willemse, Abraham J. Koster, Gilles P. van Wezel
Published in: Nature Communications, Issue 7, 2016, Page(s) ncomms11836, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/ncomms11836

Subcompartmentalization by cross-membranes during early growth of Streptomyces hyphae

Author(s): Paula Yagüe, Joost Willemse, Roman I. Koning, Beatriz Rioseras, María T. López-García, Nathaly Gonzalez-Quiñonez, Carmen Lopez-Iglesias, Pavel V. Shliaha, Adelina Rogowska-Wrzesinska, Abraham J. Koster, Ole N. Jensen, Gilles P. van Wezel, Ángel Manteca
Published in: Nature Communications, Issue 7, 2016, Page(s) 12467, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/ncomms12467

Structural Characterization of the Interaction of the Fibroblast Growth Factor Receptor with a Small Molecule Allosteric Inhibitor

Author(s): Franziska Kappert, Sridhar Sreeramulu, Hendrik R. A. Jonker, Christian Richter, Vladimir V. Rogov, Ewgenij Proschak, Bruno Hargittay, Krishna Saxena, Harald Schwalbe
Published in: Chemistry - A European Journal, Issue 24/31, 2018, Page(s) 7861-7865, ISSN 0947-6539
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/chem.201801770

Dynamic kinetochore size regulation promotes microtubule capture and chromosome biorientation in mitosis

Author(s): Carlos Sacristan, Misbha Ud Din Ahmad, Jenny Keller, Job Fermie, Vincent Groenewold, Eelco Tromer, Alexander Fish, Roberto Melero, José María Carazo, Judith Klumperman, Andrea Musacchio, Anastassis Perrakis, Geert JPL Kops
Published in: Nature Cell Biology, Issue 20/7, 2018, Page(s) 800-810, ISSN 1465-7392
Publisher: Nature Publishing Group
DOI: 10.1038/s41556-018-0130-3

Direct shape determination of intermediates in evolving macromolecular solutions from small-angle scattering data

Author(s): Petr V. Konarev, Dmitri I. Svergun
Published in: IUCrJ, Issue 5/4, 2018, Page(s) 402-409, ISSN 2052-2525
Publisher: IUCrJ
DOI: 10.1107/S2052252518005900

Smaller capillaries improve the small-angle X-ray scattering signal and sample consumption for biomacromolecular solutions

Author(s): Martin A. Schroer, Clement E. Blanchet, Andrey Yu. Gruzinov, Melissa A. Gräwert, Martha E. Brennich, Nelly R. Hajizadeh, Cy M. Jeffries, Dmitri I. Svergun
Published in: Journal of Synchrotron Radiation, Issue 25/4, 2018, Page(s) 1113-1122, ISSN 1600-5775
Publisher: IUCrJ
DOI: 10.1107/S1600577518007907

Paradoxically, Most Flexible Ligand Binds Most Entropy-Favored: Intriguing Impact of Ligand Flexibility and Solvation on Drug–Kinase Binding

Author(s): Barbara Wienen-Schmidt, Hendrik R. A. Jonker, Tobias Wulsdorf, Hans-Dieter Gerber, Krishna Saxena, Denis Kudlinzki, Sridhar Sreeramulu, Giacomo Parigi, Claudio Luchinat, Andreas Heine, Harald Schwalbe, Gerhard Klebe
Published in: Journal of Medicinal Chemistry, Issue 61/14, 2018, Page(s) 5922-5933, ISSN 0022-2623
Publisher: American Chemical Society
DOI: 10.1021/acs.jmedchem.8b00105

The domain architecture of PtkA, the first tyrosine kinase from Mycobacterium tuberculosis , differs from the conventional kinase architecture

Author(s): Anna Niesteruk, Hendrik R. A. Jonker, Christian Richter, Verena Linhard, Sridhar Sreeramulu, Harald Schwalbe
Published in: Journal of Biological Chemistry, Issue 293/30, 2018, Page(s) 11823-11836, ISSN 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.RA117.000120

Visualization of translation termination intermediates trapped by the Apidaecin 137 peptide during RF3-mediated recycling of RF1

Author(s): Michael Graf, Paul Huter, Cristina Maracci, Miroslav Peterek, Marina V. Rodnina, Daniel N. Wilson
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-05465-1

Recognition of Complex Core-Fucosylated N-Glycans by a Mini Lectin

Author(s): Aurore Cabanettes, Lukas Perkams, Carolina Spies, Carlo Unverzagt, Annabelle Varrot
Published in: Angewandte Chemie International Edition, Issue 57/32, 2018, Page(s) 10178-10181, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201805165

1H-detected solid-state NMR of proteins entrapped in bioinspired silica: a new tool for biomaterials characterization

Author(s): Enrico Ravera, Linda Cerofolini, Tommaso Martelli, Alexandra Louka, Marco Fragai, Claudio Luchinat
Published in: Scientific Reports, Issue 6, 2016, Page(s) 27851, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/srep27851

Aromatic Cluster Sensor of Protein Folding: Near-UV Electronic Circular Dichroism Bands Assigned to Fold Compactness

Author(s): Viktor Farkas, Imre Jákli, Gábor K. Tóth, András Perczel
Published in: Chemistry - A European Journal, Issue 22/39, 2016, Page(s) 13871-13883, ISSN 0947-6539
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/chem.201602455

Emergence of a Homo sapiens-specific gene family and chromosome 16p11.2 CNV susceptibility

Author(s): Xander Nuttle, Giuliana Giannuzzi, Michael H. Duyzend, Joshua G. Schraiber, Iñigo Narvaiza, Peter H. Sudmant, Osnat Penn, Giorgia Chiatante, Maika Malig, John Huddleston, Chris Benner, Francesca Camponeschi, Simone Ciofi-Baffoni, Holly A. F. Stessman, Maria C. N. Marchetto, Laura Denman, Lana Harshman, Carl Baker, Archana Raja, Kelsi Penewit, Nicolette Janke, W. Joyce Tang, Mario Ventura, Lucia B
Published in: Nature, Issue 536/7615, 2016, Page(s) 205-209, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/nature19075

Mitochondrial Bol1 and Bol3 function as assembly factors for specific iron-sulfur proteins

Author(s): Marta A Uzarska, Veronica Nasta, Benjamin D Weiler, Farah Spantgar, Simone Ciofi-Baffoni, Maria Rosaria Saviello, Leonardo Gonnelli, Ulrich Mühlenhoff, Lucia Banci, Roland Lill
Published in: eLife, Issue 5, 2016, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/eLife.16673

Plasma metabolomics in calves with acute bronchopneumonia

Author(s): Abdullah Basoglu, Nuri Baspinar, Leonardo Tenori, Alessia Vignoli, Ramazan Yildiz
Published in: Metabolomics, Issue 12/8, 2016, ISSN 1573-3882
Publisher: Springer Verlag
DOI: 10.1007/s11306-016-1074-x

EGFR Dynamics Change during Activation in Native Membranes as Revealed by NMR

Author(s): Mohammed Kaplan, Siddarth Narasimhan, Cecilia de Heus, Deni Mance, Sander van Doorn, Klaartje Houben, Dušan Popov-Čeleketić, Reinier Damman, Eugene A. Katrukha, Purvi Jain, Willie J.C. Geerts, Albert J.R. Heck, Gert E. Folkers, Lukas C. Kapitein, Simone Lemeer, Paul M.P. van Bergen en Henegouwen, Marc Baldus
Published in: Cell, Issue 167, 2016, ISSN 0092-8674
Publisher: Cell Press
DOI: 10.1016/j.cell.2016.10.038

Structural analysis of a signal peptide inside the ribosome tunnel by DNP MAS NMR

Author(s): S. Lange, W. T. Franks, N. Rajagopalan, K. Do ring, M. A. Geiger, A. Linden, B.-J. van Rossum, G. Kramer, B. Bukau, H. Oschkinat
Published in: Science Advances, Issue 2/8, 2016, Page(s) e1600379-e1600379, ISSN 2375-2548
Publisher: AAAS
DOI: 10.1126/sciadv.1600379

Resolution of ab initio shapes determined from small-angle scattering

Author(s): Anne T. Tuukkanen, Gerard J. Kleywegt, Dmitri I. Svergun
Published in: IUCrJ, Issue 3/6, 2016, Page(s) 440-447, ISSN 2052-2525
Publisher: International Union of Crystallography
DOI: 10.1107/S2052252516016018

Studying assembly of the BAM complex in native membranes by cellular solid-state NMR spectroscopy

Author(s): Cecilia Pinto, Deni Mance, Manon Julien, Mark Daniels, Markus Weingarth, Marc Baldus
Published in: Journal of Structural Biology, 2017, ISSN 1047-8477
Publisher: Academic Press
DOI: 10.1016/j.jsb.2017.11.015

Experimental and computational framework for a dynamic protein atlas of human cell division

Author(s): Yin Cai, M. Julius Hossain, Jean-Karim Hériché, Antonio Z. Politi, Nike Walther, Birgit Koch, Malte Wachsmuth, Bianca Nijmeijer, Moritz Kueblbeck, Marina Martinic-Kavur, Rene Ladurner, Stephanie Alexander, Jan-Michael Peters, Jan Ellenberg
Published in: Nature, Issue 561/7723, 2018, Page(s) 411-415, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-018-0518-z

Formation of the β-barrel assembly machinery complex in lipid bilayers as seen by solid-state NMR

Author(s): Cecilia Pinto, Deni Mance, Tessa Sinnige, Mark Daniëls, Markus Weingarth, Marc Baldus
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-06466-w

Insight into small molecule binding to the neonatal Fc receptor by X-ray crystallography and 100 kHz magic-angle-spinning NMR

Author(s): Daniel Stöppler, Alex Macpherson, Susanne Smith-Penzel, Nicolas Basse, Fabien Lecomte, Hervé Deboves, Richard D. Taylor, Tim Norman, John Porter, Lorna C. Waters, Marta Westwood, Ben Cossins, Katharine Cain, James White, Robert Griffin, Christine Prosser, Sebastian Kelm, Amy H. Sullivan, David Fox, Mark D. Carr, Alistair Henry, Richard Taylor, Beat H. Meier, Hartmut Oschkinat, Alastair D. Lawson
Published in: PLOS Biology, Issue 16/5, 2018, Page(s) e2006192, ISSN 1545-7885
Publisher: PLoS
DOI: 10.1371/journal.pbio.2006192

Structural basis for antibiotic resistance mediated by the Bacillus subtilis ABCF ATPase VmlR

Author(s): Caillan Crowe-McAuliffe, Michael Graf, Paul Huter, Hiraku Takada, Maha Abdelshahid, Jiří Nováček, Victoriia Murina, Gemma C. Atkinson, Vasili Hauryliuk, Daniel N. Wilson
Published in: Proceedings of the National Academy of Sciences, Issue 115/36, 2018, Page(s) 8978-8983, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1808535115

Multivalency regulates activity in an intrinsically disordered transcription factor

Author(s): Sarah Clark, Janette B Myers, Ashleigh King, Radovan Fiala, Jiri Novacek, Grant Pearce, Jörg Heierhorst, Steve L Reichow, Elisar J Barbar
Published in: eLife, Issue 7, 2018, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/eLife.36258

Urease Inhibition in the Presence of N -( n -Butyl)thiophosphoric Triamide, a Suicide Substrate: Structure and Kinetics

Author(s): Luca Mazzei, Michele Cianci, Umberto Contaldo, Francesco Musiani, Stefano Ciurli
Published in: Biochemistry, Issue 56/40, 2017, Page(s) 5391-5404, ISSN 0006-2960
Publisher: American Chemical Society
DOI: 10.1021/acs.biochem.7b00750

Structural and functional studies of the metalloregulator Fur identify a promoter-binding mechanism and its role in Francisella tularensis virulence

Author(s): J. Pérard, S. Nader, M. Levert, L. Arnaud, P. Carpentier, C. Siebert, F. Blanquet, C. Cavazza, P. Renesto, D. Schneider, M. Maurin, J. Coves, S. Crouzy, I. Michaud-Soret
Published in: Communications Biology, Issue 1/1, 2018, ISSN 2399-3642
Publisher: Springer Nature
DOI: 10.1038/s42003-018-0095-6

OCP–FRP protein complex topologies suggest a mechanism for controlling high light tolerance in cyanobacteria

Author(s): Nikolai N. Sluchanko, Yury B. Slonimskiy, Evgeny A. Shirshin, Marcus Moldenhauer, Thomas Friedrich, Eugene G. Maksimov
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-06195-0

The Regulation of Axon Diameter: From Axonal Circumferential Contractility to Activity-Dependent Axon Swelling

Author(s): Ana Rita Costa, Rita Pinto-Costa, Sara Castro Sousa, Mónica Mendes Sousa
Published in: Frontiers in Molecular Neuroscience, Issue 11, 2018, ISSN 1662-5099
Publisher: Frontiers Research Foundation
DOI: 10.3389/fnmol.2018.00319

Rapid access to RNA resonances by proton-detected solid-state NMR at >100 kHz MAS

Author(s): Alexander Marchanka, Jan Stanek, Guido Pintacuda, Teresa Carlomagno
Published in: Chemical Communications, Issue 54/65, 2018, Page(s) 8972-8975, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/c8cc04437f

Architecture of the complete oxygen-sensing FixL-FixJ two-component signal transduction system

Author(s): Gareth S. A. Wright, Akane Saeki, Takaaki Hikima, Yoko Nishizono, Tamao Hisano, Misaki Kamaya, Kohei Nukina, Hideo Nishitani, Hiro Nakamura, Masaki Yamamoto, Svetlana V. Antonyuk, S. Samar Hasnain, Yoshitsugu Shiro, Hitomi Sawai
Published in: Science Signaling, Issue 11/525, 2018, Page(s) eaaq0825, ISSN 1937-9145
Publisher: American Association for the Advancement of Science
DOI: 10.1126/scisignal.aaq0825

Disordered Peptides Looking for Their Native Environment: Structural Basis of CB1 Endocannabinoid Receptor Binding to Pepcans

Author(s): Alessandro Emendato, Remo Guerrini, Erika Marzola, Hans Wienk, Rolf Boelens, Serena Leone, Delia Picone
Published in: Frontiers in Molecular Biosciences, Issue 5, 2018, ISSN 2296-889X
Publisher: Frontiers
DOI: 10.3389/fmolb.2018.00100

NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family

Author(s): Alix Tröster, Stephanie Heinzlmeir, Benedict-Tilman Berger, Santosh L. Gande, Krishna Saxena, Sridhar Sreeramulu, Verena Linhard, Amir H. Nasiri, Michael Bolte, Susanne Müller, Bernhard Kuster, Guillaume Médard, Denis Kudlinzki, Harald Schwalbe
Published in: ChemMedChem, Issue 13/16, 2018, Page(s) 1629-1633, ISSN 1860-7179
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/cmdc.201800398

Structural basis of RNA polymerase I stalling at UV light-induced DNA damage

Author(s): Marta Sanz-Murillo, Jun Xu, Georgiy A. Belogurov, Olga Calvo, David Gil-Carton, María Moreno-Morcillo, Dong Wang, Carlos Fernández-Tornero
Published in: Proceedings of the National Academy of Sciences, Issue 115/36, 2018, Page(s) 8972-8977, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1802626115

On the Implication of Water on Fragment-to-Ligand Growth in Kinase Binding Thermodynamics

Author(s): Barbara Wienen-Schmidt, Tobias Wulsdorf, Hendrik R. A. Jonker, Krishna Saxena, Denis Kudlinzki, Verena Linhard, Sridhar Sreeramulu, Andreas Heine, Harald Schwalbe, Gerhard Klebe
Published in: ChemMedChem, Issue 13/18, 2018, Page(s) 1988-1996, ISSN 1860-7179
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/cmdc.201800438

Structure of Type-I Mycobacterium tuberculosis fatty acid synthase at 3.3 Å resolution

Author(s): Nadav Elad, Szilvia Baron, Yoav Peleg, Shira Albeck, Jacob Grunwald, Gal Raviv, Zippora Shakked, Oren Zimhony, Ron Diskin
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-06440-6

Homology-based loop modeling yields more complete crystallographic protein structures

Author(s): Bart van Beusekom, Krista Joosten, Maarten L. Hekkelman, Robbie P. Joosten, Anastassis Perrakis
Published in: IUCrJ, Issue 5/5, 2018, Page(s) 585-594, ISSN 2052-2525
Publisher: IUCr
DOI: 10.1107/s2052252518010552

Comment on “Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water”

Author(s): Gustavo Fuertes, Niccolo Banterle, Kiersten M. Ruff, Aritra Chowdhury, Rohit V. Pappu, Dmitri I. Svergun, Edward A. Lemke
Published in: Science, Issue 361/6405, 2018, Page(s) eaau8230, ISSN 0036-8075
Publisher: American Association for the Advancement of Science
DOI: 10.1126/science.aau8230

Tripeptide binding in a proton-dependent oligopeptide transporter

Author(s): Maria Martinez Molledo, Esben M. Quistgaard, Christian Löw
Published in: FEBS Letters, Issue 592/19, 2018, Page(s) 3239-3247, ISSN 0014-5793
Publisher: Elsevier BV
DOI: 10.1002/1873-3468.13246

Structural and biochemical characterization of the Cutibacterium acnes exo-β-1,4-mannosidase that targets the N-glycan core of host glycoproteins

Author(s): Tom Reichenbach, Dayanand Kalyani, Rosaria Gandini, Olov Svartström, Henrik Aspeborg, Christina Divne
Published in: PLOS ONE, Issue 13/9, 2018, Page(s) e0204703, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0204703

Multivariate Control of Transcript to Protein Variability in Single Mammalian Cells

Author(s): Doris Popovic, Birgit Koch, Moritz Kueblbeck, Jan Ellenberg, Lucas Pelkmans
Published in: Cell Systems, Issue 7/4, 2018, Page(s) 398-411.e6, ISSN 2405-4712
Publisher: Elsevier
DOI: 10.1016/j.cels.2018.09.001

Cryo-EM structures of human STEAP4 reveal mechanism of iron(III) reduction

Author(s): Wout Oosterheert, Laura S. van Bezouwen, Remco N. P. Rodenburg, Joke Granneman, Friedrich Förster, Andrea Mattevi, Piet Gros
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-06817-7

Subtomogram averaging of COPII assemblies reveals how coat organization dictates membrane shape

Author(s): Joshua Hutchings, Viktoriya Stancheva, Elizabeth A. Miller, Giulia Zanetti
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-06577-4

Molecular Recognition of a Thomsen-Friedenreich Antigen Mimetic Targeting Human Galectin-3

Author(s): Sabrina Santarsia, Ana Sofia Grosso, Filipa Trovão, Jesús Jiménez-Barbero, Ana Luísa Carvalho, Cristina Nativi, Filipa Marcelo
Published in: ChemMedChem, Issue 13/19, 2018, Page(s) 2030-2036, ISSN 1860-7179
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/cmdc.201800525

The first single particle analysis Map Challenge: A summary of the assessments

Author(s): J. Bernard Heymann, Roberto Marabini, Mohsen Kazemi, Carlos Oscar S. Sorzano, Maya Holmdahl, Joshua H. Mendez, Scott M. Stagg, Slavica Jonic, Eugene Palovcak, Jean-Paul Armache, Jianhua Zhao, Yifan Cheng, Grigore Pintilie, Wah Chiu, Ardan Patwardhan, Jose-Maria Carazo
Published in: Journal of Structural Biology, Issue 204/2, 2018, Page(s) 291-300, ISSN 1047-8477
Publisher: Academic Press
DOI: 10.1016/j.jsb.2018.08.010

Recombinant production of A1S_0222 from Acinetobacter baumannii ATCC 17978 and confirmation of its DNA-(adenine N6)-methyltransferase activity

Author(s): Ulrike Blaschke, Beneditta Suwono, Sachli Zafari, Ingo Ebersberger, Evelyn Skiebe, Cy M. Jeffries, Dmitri I. Svergun, Gottfried Wilharm
Published in: Protein Expression and Purification, Issue 151, 2018, Page(s) 78-85, ISSN 1046-5928
Publisher: Academic Press
DOI: 10.1016/j.pep.2018.06.009

IBA57 Recruits ISCA2 to Form a [2Fe-2S] Cluster-Mediated Complex

Author(s): Spyridon Gourdoupis, Veronica Nasta, Vito Calderone, Simone Ciofi-Baffoni, Lucia Banci
Published in: Journal of the American Chemical Society, Issue 140/43, 2018, Page(s) 14401-14412, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.8b09061

DNA recognition by Arabidopsis transcription factors ABI3 and NGA1

Author(s): Giedrius Sasnauskas, Elena Manakova, Kęstutis Lapėnas, Kotryna Kauneckaitė, Virginijus Siksnys
Published in: The FEBS Journal, Issue 285/21, 2018, Page(s) 4041-4059, ISSN 1742-464X
Publisher: Blackwell Publishing Inc.
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Probing the Architecture of a Multi-PDZ Domain Protein: Structure of PDZK1 in Solution

Author(s): Hajizadeh NR, Pieprzyk J, Skopintsev P, Flayhan A, Svergun DI, Löw C
Published in: Structure, Issue 26/11, 2018, Page(s) 1522-1533.e5, ISSN 0969-2126
Publisher: Elsevier
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Structure of Mutant Hepatitis B Core Protein Capsids with Premature Secretion Phenotype

Author(s): Bettina Böttcher, Michael Nassal
Published in: Journal of Molecular Biology, Issue 430/24, 2018, Page(s) 4941-4954, ISSN 0022-2836
Publisher: Academic Press
DOI: 10.1016/j.jmb.2018.10.018

Map challenge: Analysis using a pair comparison method based on Fourier shell correlation

Author(s): R. Marabini, M. Kazemi, C.O.S. Sorzano, J.M. Carazo
Published in: Journal of Structural Biology, Issue 204/3, 2018, Page(s) 527-542, ISSN 1047-8477
Publisher: Academic Press
DOI: 10.1016/j.jsb.2018.09.009

A tumor-targeted trimeric 4-1BB-agonistic antibody induces potent anti-tumor immunity without systemic toxicity

Author(s): Marta Compte, Seandean Lykke Harwood, Ines G. Muñoz, Rocio Navarro, Manuela Zonca, Gema Perez-Chacon, Ainhoa Erce-Llamazares, Nekane Merino, Antonio Tapia-Galisteo, Angel M. Cuesta, Kasper Mikkelsen, Eduardo Caleiras, Natalia Nuñez-Prado, M. Angela Aznar, Simon Lykkemark, Jorge Martínez-Torrecuadrada, Ignacio Melero, Francisco J. Blanco, Jorge Bernardino de la Serna, Juan M. Zapata, Laura Sanz,
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-07195-w

Function and Interactions of ERCC1-XPF in DNA Damage Response

Author(s): Maryam Faridounnia, Gert Folkers, Rolf Boelens
Published in: Molecules, Issue 23/12, 2018, Page(s) 3205, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules23123205

Assessing protein conformational landscapes: integration of DEER data in Maximum Occurrence analysis

Author(s): Lucia Gigli, Witold Andrałojć, Arina Dalaloyan, Giacomo Parigi, Enrico Ravera, Daniella Goldfarb, Claudio Luchinat
Published in: Physical Chemistry Chemical Physics, Issue 20/43, 2018, Page(s) 27429-27438, ISSN 1463-9076
Publisher: Royal Society of Chemistry
DOI: 10.1039/c8cp06195e

Local and Global Dynamics in Intrinsically Disordered Synuclein

Author(s): Nasrollah Rezaei-Ghaleh, Giacomo Parigi, Andrea Soranno, Andrea Holla, Stefan Becker, Benjamin Schuler, Claudio Luchinat, Markus Zweckstetter
Published in: Angewandte Chemie International Edition, Issue 57/46, 2018, Page(s) 15262-15266, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201808172

Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR

Author(s): Wing Ying Chow, Rui Li, Ieva Goldberga, David G. Reid, Rakesh Rajan, Jonathan Clark, Hartmut Oschkinat, Melinda J. Duer, Robert Hayward, Catherine M. Shanahan
Published in: Chemical Communications, Issue 54/89, 2018, Page(s) 12570-12573, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/c8cc04960b

Using Scipion for stream image processing at Cryo-EM facilities

Author(s): J. Gómez-Blanco, J.M. de la Rosa-Trevín, R. Marabini, L. del Cano, A. Jiménez, M. Martínez, R. Melero, T. Majtner, D. Maluenda, J. Mota, Y. Rancel, E Ramírez-Aportela, J.L. Vilas, M. Carroni, S. Fleischmann, E. Lindahl, A.W. Ashton, M. Basham, D.K. Clare, K. Savage, C.A. Siebert, G.G. Sharov, C.O.S. Sorzano, P. Conesa, J.M. Carazo
Published in: Journal of Structural Biology, Issue 204/3, 2018, Page(s) 457-463, ISSN 1047-8477
Publisher: Academic Press
DOI: 10.1016/j.jsb.2018.10.001

Allosteric Inhibition of a Mammalian Lectin

Author(s): Jonas Aretz, Upendra R. Anumala, Felix F. Fuchsberger, Narges Molavi, Nandor Ziebart, Hengxi Zhang, Marc Nazaré, Christoph Rademacher
Published in: Journal of the American Chemical Society, Issue 140/44, 2018, Page(s) 14915-14925, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.8b08644

β 2 -Type Amyloidlike Fibrils of Poly- l -glutamic Acid Convert into Long, Highly Ordered Helices upon Dissolution in Dimethyl Sulfoxide

Author(s): Sylwia Berbeć, Robert Dec, Dmitry Molodenskiy, Beata Wielgus-Kutrowska, Christian Johannessen, Agnieszka Hernik-Magoń, Fernando Tobias, Agnieszka Bzowska, Grzegorz Ścibisz, Timothy A. Keiderling, Dmitri Svergun, Wojciech Dzwolak
Published in: The Journal of Physical Chemistry B, Issue 122/50, 2018, Page(s) 11895-11905, ISSN 1520-6106
Publisher: American Chemical Society
DOI: 10.1021/acs.jpcb.8b08308

Biochemical and structural studies reveal differences and commonalities among cap-snatching endonucleases from segmented negative-strand RNA viruses

Author(s): Tobias Holm, Janine-Denise Kopicki, Carola Busch, Silke Olschewski, Maria Rosenthal, Charlotte Uetrecht, Stephan Günther, Sophia Reindl
Published in: Journal of Biological Chemistry, Issue 293/51, 2018, Page(s) 19686-19698, ISSN 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.ra118.004373

Protein Glycosylation through Sulfur Fluoride Exchange (SuFEx) Chemistry: The Key Role of a Fluorosulfate Thiolactoside

Author(s): Alberto Marra, Jiajia Dong, Tiancheng Ma, Stefano Giuntini, Elisa Crescenzo, Linda Cerofolini, Marco Martinucci, Claudio Luchinat, Marco Fragai, Cristina Nativi, Alessandro Dondoni
Published in: Chemistry - A European Journal, Issue 24/71, 2018, Page(s) 18981-18987, ISSN 0947-6539
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/chem.201803912

Effects of Boron Supplementation on Peripartum Dairy Cows' Health.

Author(s): Abdullah Basoglu, Nuri Baspinar, Leonardo Tenori, Alessia Vignoli, Erdem Gulersoy
Published in: Biological Trace Element Research, 2017, ISSN 0163-4984
Publisher: Humana Press, Inc.
DOI: 10.1007/s12011-017-0971-9

Direct assessment of substrate binding to the Neurotransmitter:Sodium Symporter LeuT by solid state NMR

Author(s): Simon Erlendsson, Kamil Gotfryd, Flemming Hofmann, Larsen Jonas, Sigurd Mortensen, Michel-Andreas Geiger, Barth-Jan van Rossum, Hartmut Oschkinat, Ulrik Gether, Kaare Teilum, Claus J Loland
Published in: eLife, 2017, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/eLife.19314.001

Cryo-EM structure of the spinach chloroplast ribosome reveals the location of plastid-specific ribosomal proteins and extensions

Author(s): Michael Graf, Stefan Arenz, Paul Huter, Alexandra Dönhöfer, Jiří Nováček, Daniel N. Wilson
Published in: Nucleic Acids Research, 2017, Page(s) gkw1272, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkw1272

Structural Mechanism for Cargo Recognition by the Retromer Complex

Author(s): Marıa Lucas, David C. Gershlick, Ander Vidaurrazaga, Adriana L. Rojas, Juan S. Bonifacino, Aitor Hierro
Published in: Cell, 2016, ISSN 0092-8674
Publisher: Cell Press
DOI: 10.1016/j.cell.2016.10.056

Structure determination of a major facilitator peptide transporter: Inward facing PepTSt from Streptococcus thermophilus crystallized in space group P3121

Author(s): Esben M. Quistgaard, Maria Martinez Molledo, Christian Löw
Published in: PLOS one, 2017, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0173126

[4Fe-4S] Cluster Assembly in Mitochondria and Its Impairment by Copper

Author(s): Diego Brancaccio, Angelo Gallo, Mario Piccioli, Ettore Novellino, Simone Ciofi-Baffoni, Lucia Banci
Published in: Journal of the American Chemical Society, 2016, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.6b09567

Gelified Biofluids for High-Resolution Magic Angle Spinning 1H NMR Analysis: The Case of Urine

Author(s): Panteleimon G. Takis, Leonardo Tenori, Enrico Ravera, Claudio Luchinat
Published in: Analytical Chemistry, 2017, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.6b04318

Three-Dimensional Structure of Full-Length NtrX, an Unusual Member of the NtrC Family of Response Regulators

Author(s): Ignacio Fernández, Irina Cornaciu, Mariela del Carmen Carrica, Emiko Uchikawa, Guillaume Hoffmann, Rodrigo Sieira, José Antonio Márquez, Fernando A. Goldbaum
Published in: Journal of Molecular Biology, 2017, ISSN 0022-2836
Publisher: Academic Press
DOI: 10.1016/j.jmb.2016.12.022

Decision-Making in Cascade Complexes Harboring crRNAs of Altered Length

Author(s): Inga Songailiene, Marius Rutkauskas, Tomas Sinkunas, Elena Manakova, Sabine Wittig, Carla Schmidt, Virginijus Siksnys, Ralf Seidel
Published in: Cell Reports, Issue 28/12, 2019, Page(s) 3157-3166.e4, ISSN 2211-1247
Publisher: Cell Press
DOI: 10.1016/j.celrep.2019.08.033

Engineered Carbonic Anhydrase VI-Mimic Enzyme Switched the Structure and Affinities of Inhibitors

Author(s): Justina Kazokaitė, Visvaldas Kairys, Joana Smirnovienė, Alexey Smirnov, Elena Manakova, Martti Tolvanen, Seppo Parkkila, Daumantas Matulis
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-019-49094-0

Cryo-EM structure of pleconaril-resistant rhinovirus-B5 complexed to the antiviral OBR-5-340 reveals unexpected binding site

Author(s): Jiri Wald, Marion Pasin, Martina Richter, Christin Walther, Neann Mathai, Johannes Kirchmair, Vadim A. Makarov, Nikolaus Goessweiner-Mohr, Thomas C. Marlovits, Irene Zanella, Antonio Real-Hohn, Nuria Verdaguer, Dieter Blaas, Michaela Schmidtke
Published in: Proceedings of the National Academy of Sciences, Issue 116/38, 2019, Page(s) 19109-19115, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1904732116

What are the methodological and theoretical prospects for paramagnetic NMR in structural biology? A glimpse into the crystal ball

Author(s): Enrico Ravera, Giacomo Parigi, Claudio Luchinat
Published in: Journal of Magnetic Resonance, Issue 306, 2019, Page(s) 173-179, ISSN 1090-7807
Publisher: Academic Press
DOI: 10.1016/j.jmr.2019.07.027

Atomic structure of the Epstein-Barr virus portal

Author(s): Cristina Machón, Montserrat Fàbrega-Ferrer, Daming Zhou, Ana Cuervo, José L. Carrascosa, David I. Stuart, Miquel Coll
Published in: Nature Communications, Issue 10/1, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-019-11706-8

Monitoring DNA–Ligand Interactions in Living Human Cells Using NMR Spectroscopy

Author(s): Michaela Krafcikova, Simon Dzatko, Coralie Caron, Anton Granzhan, Radovan Fiala, Tomas Loja, Marie-Paule Teulade-Fichou, Tomas Fessl, Robert Hänsel-Hertsch, Jean-Louis Mergny, Silvie Foldynova-Trantirkova, Lukas Trantirek
Published in: Journal of the American Chemical Society, Issue 141/34, 2019, Page(s) 13281-13285, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.9b03031

Crystal structures of cholera toxin in complex with fucosylated receptors point to importance of secondary binding site

Author(s): Joel B. Heim, Vesna Hodnik, Julie E. Heggelund, Gregor Anderluh, Ute Krengel
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-019-48579-2

Detection of nucleic acids and other low abundance components in native bone and osteosarcoma extracellular matrix by isotope enrichment and DNP-enhanced NMR

Author(s): Ieva Goldberga, Rui Li, Wing Ying Chow, David G. Reid, Ulyana Bashtanova, Rakesh Rajan, Anna Puszkarska, Hartmut Oschkinat, Melinda J. Duer
Published in: RSC Advances, Issue 9/46, 2019, Page(s) 26686-26690, ISSN 2046-2069
Publisher: Royal Society of Chemistry
DOI: 10.1039/c9ra03198g

Molecular determinants of the mechanism and substrate specificity of Clostridium difficile proline-proline endopeptidase-1

Author(s): Christian Pichlo, Linda Juetten, Fabian Wojtalla, Magdalena Schacherl, Dolores Diaz, Ulrich Baumann
Published in: Journal of Biological Chemistry, Issue 294/30, 2019, Page(s) 11525-11535, ISSN 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.ra119.009029

Structures of T7 bacteriophage portal and tail suggest a viral DNA retention and ejection mechanism

Author(s): Ana Cuervo, Montserrat Fàbrega-Ferrer, Cristina Machón, José Javier Conesa, Francisco J. Fernández, Rosa Pérez-Luque, Mar Pérez-Ruiz, Joan Pous, M. Cristina Vega, José L. Carrascosa, Miquel Coll
Published in: Nature Communications, Issue 10/1, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-019-11705-9

Validation of electron microscopy initial models via small angle X-ray scattering curves

Author(s): Amaya Jiménez, Slavica Jonic, Tomas Majtner, Joaquín Otón, Jose Luis Vilas, David Maluenda, Javier Mota, Erney Ramírez-Aportela, Marta Martínez, Yaiza Rancel, Joan Segura, Ruben Sánchez-García, Roberto Melero, Laura del Cano, Pablo Conesa, Lars Skjaerven, Roberto Marabini, Jose M Carazo, Carlos Oscar S Sorzano
Published in: Bioinformatics, Issue 35/14, 2018, Page(s) 2427-2433, ISSN 1367-4803
Publisher: Oxford University Press
DOI: 10.1093/bioinformatics/bty985

Integrative Approaches in Structural Biology: A More Complete Picture from the Combination of Individual Techniques

Author(s): Linda Cerofolini, Marco Fragai, Enrico Ravera, Christoph A. Diebolder, Ludovic Renault, Vito Calderone
Published in: Biomolecules, Issue 9/8, 2019, Page(s) 370, ISSN 2218-273X
Publisher: -
DOI: 10.3390/biom9080370

Structural Basis of Transcription: RNA Polymerase Backtracking and Its Reactivation

Author(s): Mo’men Abdelkareem, Charlotte Saint-André, Maria Takacs, Gabor Papai, Corinne Crucifix, Xieyang Guo, Julio Ortiz, Albert Weixlbaumer
Published in: Molecular Cell, Issue 75/2, 2019, Page(s) 298-309.e4, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2019.04.029

Molecular tuning of farnesoid X receptor partial agonism

Author(s): Daniel Merk, Sridhar Sreeramulu, Denis Kudlinzki, Krishna Saxena, Verena Linhard, Santosh L. Gande, Fabian Hiller, Christina Lamers, Ewa Nilsson, Anna Aagaard, Lisa Wissler, Niek Dekker, Krister Bamberg, Manfred Schubert-Zsilavecz, Harald Schwalbe
Published in: Nature Communications, Issue 10/1, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-019-10853-2

High-throughput stability screening for detergent-solubilized membrane proteins

Author(s): Vadim Kotov, Kim Bartels, Katharina Veith, Inokentijs Josts, Udaya K. Tiruttani Subhramanyam, Christian Günther, Jörg Labahn, Thomas C. Marlovits, Isabel Moraes, Henning Tidow, Christian Löw, Maria M. Garcia-Alai
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-019-46686-8

DNP-Supported Solid-State NMR Spectroscopy of Proteins Inside Mammalian Cells

Author(s): Siddarth Narasimhan, Stephan Scherpe, Alessandra Lucini Paioni, Johan van der Zwan, Gert E. Folkers, Huib Ovaa, Marc Baldus
Published in: Angewandte Chemie International Edition, Issue 58/37, 2019, Page(s) 12969-12973, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
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Joint X-ray/NMR structure refinement of multidomain/multisubunit systems

Author(s): Azzurra Carlon, Enrico Ravera, Giacomo Parigi, Garib N. Murshudov, Claudio Luchinat
Published in: Journal of Biomolecular NMR, Issue 73/6-7, 2019, Page(s) 265-278, ISSN 0925-2738
Publisher: Kluwer Academic Publishers
DOI: 10.1007/s10858-018-0212-3

High-throughput peptide-MHC complex generation and kinetic screenings of TCRs with peptide-receptive HLA-A*02:01 molecules

Author(s): Andreas Moritz, Raghavendra Anjanappa, Claudia Wagner, Sebastian Bunk, Martin Hofmann, Gabriele Pszolla, Ankur Saikia, Maria Garcia-Alai, Rob Meijers, Hans-Georg Rammensee, Sebastian Springer, Dominik Maurer
Published in: Science Immunology, Issue 4/37, 2019, Page(s) eaav0860, ISSN 2470-9468
Publisher: -
DOI: 10.1126/sciimmunol.aav0860

Empty peptide-receptive MHC class I molecules for efficient detection of antigen-specific T cells

Author(s): Sunil Kumar Saini, Tripti Tamhane, Raghavendra Anjanappa, Ankur Saikia, Sofie Ramskov, Marco Donia, Inge Marie Svane, Søren Nyboe Jakobsen, Maria Garcia-Alai, Martin Zacharias, Rob Meijers, Sebastian Springer, Sine Reker Hadrup
Published in: Science Immunology, Issue 4/37, 2019, Page(s) eaau9039, ISSN 2470-9468
Publisher: -
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Absolute quantification of cohesin, CTCF and their regulators in human cells

Author(s): Johann Holzmann, Antonio Z Politi, Kota Nagasaka, Merle Hantsche-Grininger, Nike Walther, Birgit Koch, Johannes Fuchs, Gerhard Dürnberger, Wen Tang, Rene Ladurner, Roman R Stocsits, Georg A Busslinger, Béla Novák, Karl Mechtler, Iain Finley Davidson, Jan Ellenberg, Jan-Michael Peters
Published in: eLife, Issue 8, 2019, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.46269

A novel druggable interprotomer pocket in the capsid of rhino- and enteroviruses

Author(s): Rana Abdelnabi, James A. Geraets, Yipeng Ma, Carmen Mirabelli, Justin W. Flatt, Aušra Domanska, Leen Delang, Dirk Jochmans, Timiri Ajay Kumar, Venkatesan Jayaprakash, Barij Nayan Sinha, Pieter Leyssen, Sarah J. Butcher, Johan Neyts
Published in: PLOS Biology, Issue 17/6, 2019, Page(s) e3000281, ISSN 1545-7885
Publisher: -
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Determining cellular CTCF and cohesin abundances to constrain 3D genome models

Author(s): Claudia Cattoglio, Iryna Pustova, Nike Walther, Jaclyn J Ho, Merle Hantsche-Grininger, Carla J Inouye, M Julius Hossain, Gina M Dailey, Jan Ellenberg, Xavier Darzacq, Robert Tjian, Anders S Hansen
Published in: eLife, Issue 8, 2019, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.40164

Author(s): Arne Raasakka, Salla Ruskamo, Robert Barker, Oda C. Krokengen, Guro H. Vatne, Cecilie K. Kristiansen, Erik I. Hallin, Maximilian W. A. Skoda, Ulrich Bergmann, Hanna Wacklin-Knecht, Nykola C. Jones, Søren V. Hoffmann, Petri Kursula
Published in: PLOS ONE, Issue 14/6, 2019, Page(s) e0216833, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0216833

An Emerin LEM-Domain Mutation Impairs Cell Response to Mechanical Stress

Author(s): Nada Essawy, Camille Samson, Ambre Petitalot, Sophie Moog, Anne Bigot, Isaline Herrada, Agathe Marcelot, Ana-Andreea Arteni, Catherine Coirault, Sophie Zinn-Justin
Published in: Cells, Issue 8/6, 2019, Page(s) 570, ISSN 2073-4409
Publisher: -
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Protective Dps–DNA co‐crystallization in stressed cells: an in vitro structural study by small‐angle X‐ray scattering and cryo‐electron tomography

Author(s): Liubov A. Dadinova, Yurii M. Chesnokov, Roman A. Kamyshinsky, Ivan A. Orlov, Maxim V. Petoukhov, Andrey A. Mozhaev, Ekaterina Yu. Soshinskaya, Vassili N. Lazarev, Valentin A. Manuvera, Anton S. Orekhov, Alexander L. Vasiliev, Eleonora V. Shtykova
Published in: FEBS Letters, Issue 593/12, 2019, Page(s) 1360-1371, ISSN 0014-5793
Publisher: Elsevier BV
DOI: 10.1002/1873-3468.13439

The structure of a membrane adenylyl cyclase bound to an activated stimulatory G protein

Author(s): Chao Qi, Simona Sorrentino, Ohad Medalia, Volodymyr M. Korkhov
Published in: Science, Issue 364/6438, 2019, Page(s) 389-394, ISSN 0036-8075
Publisher: American Association for the Advancement of Science
DOI: 10.1126/science.aav0778

Side chain to main chain hydrogen bonds stabilize a polyglutamine helix in a transcription factor

Author(s): Albert Escobedo, Busra Topal, Micha B. A. Kunze, Juan Aranda, Giulio Chiesa, Daniele Mungianu, Ganeko Bernardo-Seisdedos, Bahareh Eftekharzadeh, Margarida Gairí, Roberta Pierattelli, Isabella C. Felli, Tammo Diercks, Oscar Millet, Jesús García, Modesto Orozco, Ramon Crehuet, Kresten Lindorff-Larsen, Xavier Salvatella
Published in: Nature Communications, Issue 10/1, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-019-09923-2

A Tail-Based Mechanism Drives Nucleosome Demethylation by the LSD2/NPAC Multimeric Complex

Author(s): Chiara Marabelli, Biagina Marrocco, Simona Pilotto, Sagar Chittori, Sarah Picaud, Sara Marchese, Giuseppe Ciossani, Federico Forneris, Panagis Filippakopoulos, Guy Schoehn, Daniela Rhodes, Sriram Subramaniam, Andrea Mattevi
Published in: Cell Reports, Issue 27/2, 2019, Page(s) 387-399.e7, ISSN 2211-1247
Publisher: Cell Press
DOI: 10.1016/j.celrep.2019.03.061

Structural characterization of a protein adsorbed on aluminum hydroxide adjuvant in vaccine formulation

Author(s): Linda Cerofolini, Stefano Giuntini, Enrico Ravera, Claudio Luchinat, Francesco Berti, Marco Fragai
Published in: npj Vaccines, Issue 4/1, 2019, ISSN 2059-0105
Publisher: -
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The carbon monoxide dehydrogenase accessory protein CooJ is a histidine-rich multidomain dimer containing an unexpected Ni(II)-binding site

Author(s): Marila Alfano, Julien Pérard, Philippe Carpentier, Christian Basset, Barbara Zambelli, Jennifer Timm, Serge Crouzy, Stefano Ciurli, Christine Cavazza
Published in: Journal of Biological Chemistry, Issue 294/19, 2019, Page(s) 7601-7614, ISSN 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.ra119.008011

Structural Basis for the Action of an All-Purpose Transcription Anti-termination Factor

Author(s): Ferdinand Krupp, Nelly Said, Yong-Heng Huang, Bernhard Loll, Jörg Bürger, Thorsten Mielke, Christian M.T. Spahn, Markus C. Wahl
Published in: Molecular Cell, Issue 74/1, 2019, Page(s) 143-157.e5, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2019.01.016

High-Resolution Crystal Structure of Endoplasmic Reticulum Aminopeptidase 1 with Bound Phosphinic Transition-State Analogue Inhibitor

Author(s): Petros Giastas, Margarete Neu, Paul Rowland, Efstratios Stratikos
Published in: ACS Medicinal Chemistry Letters, Issue 10/5, 2019, Page(s) 708-713, ISSN 1948-5875
Publisher: American Chemical Society
DOI: 10.1021/acsmedchemlett.9b00002

New insights into the tetrameric family of the Fur metalloregulators

Author(s): S. Nader, J. Pérard, P. Carpentier, L. Arnaud, S. Crouzy, I. Michaud-Soret
Published in: BioMetals, Issue 32/3, 2019, Page(s) 501-519, ISSN 0966-0844
Publisher: Kluwer Academic Publishers
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MXCuBE2 : the dawn of MXCuBE Collaboration

Author(s): Marcus Oscarsson, Antonia Beteva, David Flot, Elspeth Gordon, Matias Guijarro, Gordon Leonard, Sean McSweeney, Stephanie Monaco, Christoph Mueller-Dieckmann, Max Nanao, Didier Nurizzo, Alexander N. Popov, David von Stetten, Olof Svensson, Vicente Rey-Bakaikoa, Idrissou Chado, Leonard M. G. Chavas, Laurent Gadea, Patrick Gourhant, Tatiana Isabet, Pierre Legrand, Martin Savko, Serena Sirigu, William
Published in: Journal of Synchrotron Radiation, Issue 26/2, 2019, Page(s) 393-405, ISSN 1600-5775
Publisher: -
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Cryo-EM fibril structures from systemic AA amyloidosis reveal the species complementarity of pathological amyloids

Author(s): Falk Liberta, Sarah Loerch, Matthies Rennegarbe, Angelika Schierhorn, Per Westermark, Gunilla T. Westermark, Bouke P. C. Hazenberg, Nikolaus Grigorieff, Marcus Fändrich, Matthias Schmidt
Published in: Nature Communications, Issue 10/1, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-019-09033-z

Cryo-EM structure of a light chain-derived amyloid fibril from a patient with systemic AL amyloidosis

Author(s): Lynn Radamaker, Yin-Hsi Lin, Karthikeyan Annamalai, Stefanie Huhn, Ute Hegenbart, Stefan O. Schönland, Günter Fritz, Matthias Schmidt, Marcus Fändrich
Published in: Nature Communications, Issue 10/1, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-019-09032-0

Structure of Prototypic Peptide Transporter DtpA from E. coli in Complex with Valganciclovir Provides Insights into Drug Binding of Human PepT1

Author(s): Yonca Ural-Blimke, Ali Flayhan, Jan Strauss, Vasileios Rantos, Kim Bartels, Rolf Nielsen, Els Pardon, Jan Steyaert, Jan Kosinski, Esben M. Quistgaard, Christian Löw
Published in: Journal of the American Chemical Society, Issue 141/6, 2019, Page(s) 2404-2412, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.8b11343

Design, Synthesis and Biological Evaluation of Isoxazole-Based CK1 Inhibitors Modified with Chiral Pyrrolidine Scaffolds

Author(s): Andreas Luxenburger, Dorian Schmidt, Chiara Ianes, Christian Pichlo, Marc Krüger, Thorsten von Drathen, Elena Brunstein, Graeme Gainsford, Ulrich Baumann, Uwe Knippschild, Christian Peifer
Published in: Molecules, Issue 24/5, 2019, Page(s) 873, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules24050873

A post-translational modification of human Norovirus capsid protein attenuates glycan binding

Author(s): Alvaro Mallagaray, Robert Creutznacher, Jasmin Dülfer, Philipp H. O. Mayer, Lena Lisbeth Grimm, Jose Maria Orduña, Esben Trabjerg, Thilo Stehle, Kasper D. Rand, Bärbel S. Blaum, Charlotte Uetrecht, Thomas Peters
Published in: Nature Communications, Issue 10/1, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-019-09251-5

Impact of lipid binding on the tertiary structure and allergenic potential of Jug r 3, the non-specific lipid transfer protein from walnut

Author(s): Pawel Dubiela, Rebecca Del Conte, Francesca Cantini, Tomasz Borowski, Roberta Aina, Christian Radauer, Merima Bublin, Karin Hoffmann-Sommergruber, Stefano Alessandri
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-019-38563-1

A framework for tracer-based metabolism in mammalian cells by NMR

Author(s): Raquel Saborano, Zuhal Eraslan, Jennie Roberts, Farhat L. Khanim, Patricia F. Lalor, Michelle A. C. Reed, Ulrich L. Günther
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-018-37525-3

Building and rebuilding N-glycans in protein structure models

Author(s): Bart van Beusekom, Natasja Wezel, Maarten L. Hekkelman, Anastassis Perrakis, Paul Emsley, Robbie P. Joosten
Published in: Acta Crystallographica Section D Structural Biology, Issue 75/4, 2019, Page(s) 416-425, ISSN 2059-7983
Publisher: -
DOI: 10.1107/s2059798319003875

Structural basis for assembly of vertical single β-barrel viruses

Author(s): Isaac Santos-Pérez, Diego Charro, David Gil-Carton, Mikel Azkargorta, Felix Elortza, Dennis H. Bamford, Hanna M. Oksanen, Nicola G. A. Abrescia
Published in: Nature Communications, Issue 10/1, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-019-08927-2

Real-Time Insights into Biological Events: In-Cell Processes and Protein-Ligand Interactions

Author(s): Linda Cerofolini, Stefano Giuntini, Letizia Barbieri, Matteo Pennestri, Anna Codina, Marco Fragai, Lucia Banci, Enrico Luchinat, Enrico Ravera
Published in: Biophysical Journal, Issue 116/2, 2019, Page(s) 239-247, ISSN 0006-3495
Publisher: Biophysical Society
DOI: 10.1016/j.bpj.2018.11.3132

Cadmium effects on superoxide dismutase 1 in human cells revealed by NMR

Author(s): Panagis Polykretis, Francesca Cencetti, Chiara Donati, Enrico Luchinat, Lucia Banci
Published in: Redox Biology, Issue 21, 2019, Page(s) 101102, ISSN 2213-2317
Publisher: Elsevier BV
DOI: 10.1016/j.redox.2019.101102

A 2.8-Angstrom-Resolution Cryo-Electron Microscopy Structure of Human Parechovirus 3 in Complex with Fab from a Neutralizing Antibody

Author(s): Aušra Domanska, Justin W. Flatt, Joonas J. J. Jukonen, James A. Geraets, Sarah J. Butcher
Published in: Journal of Virology, Issue 93/4, 2019, ISSN 0022-538X
Publisher: American Society for Microbiology
DOI: 10.1128/jvi.01597-18

Regulation of Human Hsc70 ATPase and Chaperone Activities by Apg2: Role of the Acidic Subdomain

Author(s): Yovana Cabrera, Leire Dublang, José Angel Fernández-Higuero, David Albesa-Jové, Maria Lucas, Ana Rosa Viguera, Marcelo E. Guerin, Jose M.G. Vilar, Arturo Muga, Fernando Moro
Published in: Journal of Molecular Biology, Issue 431/2, 2019, Page(s) 444-461, ISSN 0022-2836
Publisher: Academic Press
DOI: 10.1016/j.jmb.2018.11.026

Utilization of Staphylococcal Immune Evasion Protein Sbi as a Novel Vaccine Adjuvant

Author(s): Yi Yang, Catherine R. Back, Melissa A. Gräwert, Ayla A. Wahid, Harriet Denton, Rebecca Kildani, Joshua Paulin, Kristin Wörner, Wolgang Kaiser, Dmitri I. Svergun, Asel Sartbaeva, Andrew G. Watts, Kevin J. Marchbank, Jean M. H. van den Elsen
Published in: Frontiers in Immunology, Issue 9, 2019, ISSN 1664-3224
Publisher: -
DOI: 10.3389/fimmu.2018.03139

Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopy

Author(s): C. O. S. Sorzano, A. Jiménez, J. Mota, J. L. Vilas, D. Maluenda, M. Martínez, E. Ramírez-Aportela, T. Majtner, J. Segura, R. Sánchez-García, Y. Rancel, L. del Caño, P. Conesa, R. Melero, S. Jonic, J. Vargas, F. Cazals, Z. Freyberg, J. Krieger, I. Bahar, R. Marabini, J. M. Carazo
Published in: Acta Crystallographica Section F Structural Biology Communications, Issue 75/1, 2019, Page(s) 19-32, ISSN 2053-230X
Publisher: John Wiley and Sons Ltd
DOI: 10.1107/s2053230x18015108

Metal cofactors trafficking and assembly in the cell: a molecular view

Author(s): Francesca Camponeschi, Lucia Banci
Published in: Pure and Applied Chemistry, Issue 91/2, 2019, Page(s) 231-245, ISSN 0033-4545
Publisher: International Union of Pure and Applied Chemistry
DOI: 10.1515/pac-2018-0720

Conformational switch in the ribosomal protein S1 guides unfolding of structured RNAs for translation initiation

Author(s): Nusrat Shahin Qureshi, Jasleen Kaur Bains, Sridhar Sreeramulu, Harald Schwalbe, Boris Fürtig
Published in: Nucleic Acids Research, 2018, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gky746

Correlative cryo super-resolution light and electron microscopy on mammalian cells using fluorescent proteins

Author(s): Maarten W. Tuijtel, Abraham J. Koster, Stefan Jakobs, Frank G. A. Faas, Thomas H. Sharp
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-018-37728-8

GM130 and p115 play a key role in the organisation of the early secretory pathway during skeletal muscle differentiation

Author(s): Emiliana Giacomello, Paolo Ronchi, Rainer Pepperkok
Published in: Journal of Cell Science, Issue 132/2, 2019, Page(s) jcs222083, ISSN 0021-9533
Publisher: The Company of Biologists Ltd.
DOI: 10.1242/jcs.222083

Shedding Light on the Interaction of Human Anti-Apoptotic Bcl-2 Protein with Ligands through Biophysical and in Silico Studies

Author(s): Joao Ramos, Jayaraman Muthukumaran, Filipe Freire, João Paquete-Ferreira, Ana Otrelo-Cardoso, Dmitri Svergun, Alejandro Panjkovich, Teresa Santos-Silva
Published in: International Journal of Molecular Sciences, Issue 20/4, 2019, Page(s) 860, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms20040860

NMR Characterization of Long‐Range Contacts in Intrinsically Disordered Proteins from Paramagnetic Relaxation Enhancement in 13 C Direct‐Detection Experiments

Author(s): Borja Mateos, Robert Konrat, Roberta Pierattelli, Isabella C. Felli
Published in: ChemBioChem, Issue 20/3, 2018, Page(s) 335-339, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201800539

3DBIONOTES v3.0: crossing molecular and structural biology data with genomic variations

Author(s): Joan Segura, Ruben Sanchez-Garcia, C O S Sorzano, J M Carazo
Published in: Bioinformatics, Issue 35/18, 2019, Page(s) 3512-3513, ISSN 1367-4803
Publisher: Oxford University Press
DOI: 10.1093/bioinformatics/btz118

DeepRes : a new deep-learning- and aspect-based local resolution method for electron-microscopy maps

Author(s): Erney Ramírez-Aportela, Javier Mota, Pablo Conesa, Jose Maria Carazo, Carlos Oscar S. Sorzano
Published in: IUCrJ, Issue 6/6, 2019, ISSN 2052-2525
Publisher: -
DOI: 10.1107/s2052252519011692

Automatic local resolution-based sharpening of cryo-EM maps

Author(s): Erney Ramírez-Aportela, Jose Luis Vilas, Alisa Glukhova, Roberto Melero, Pablo Conesa, Marta Martínez, David Maluenda, Javier Mota, Amaya Jiménez, Javier Vargas, Roberto Marabini, Patrick M Sexton, Jose Maria Carazo, Carlos Oscar S Sorzano
Published in: Bioinformatics, 2019, ISSN 1367-4803
Publisher: Oxford University Press
DOI: 10.1093/bioinformatics/btz671

Advances in image processing for single-particle analysis by electron cryomicroscopy and challenges ahead

Author(s): J.L. Vilas, N. Tabassum, J. Mota, D. Maluenda, A. Jiménez-Moreno, T. Majtner, J.M. Carazo, S.T. Acton, C.O.S. Sorzano
Published in: Current Opinion in Structural Biology, Issue 52, 2018, Page(s) 127-145, ISSN 0959-440X
Publisher: Elsevier BV
DOI: 10.1016/j.sbi.2018.11.004

Correction: Structural plasticity of the N-terminal capping helix of the TPR domain of kinesin light chain

Author(s): The Quyen Nguyen, Mélanie Chenon, Fernando Vilela, Christophe Velours, Magali Aumont-Nicaise, Jessica Andreani, Paloma F. Varela, Paola Llinas, Julie Ménétrey
Published in: PLOS ONE, Issue 13/5, 2018, Page(s) e0197193, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0197193

Advances in cryo-electron tomography for biology and medicine

Author(s): Roman I. Koning, Abraham J. Koster, Thomas H. Sharp
Published in: Annals of Anatomy - Anatomischer Anzeiger, Issue 217, 2018, Page(s) 82-96, ISSN 0940-9602
Publisher: Elsevier BV
DOI: 10.1016/j.aanat.2018.02.004

NMR Study of Rcf2 Reveals an Unusual Dimeric Topology in Detergent Micelles

Author(s): Shu Zhou, Pontus Pettersson, Peter Brzezinski, Pia Ädelroth, Lena Mäler
Published in: ChemBioChem, Issue 19/5, 2018, Page(s) 444-447, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201700664

XTEND: Extending the depth of field in cryo soft X-ray tomography

Author(s): Joaquín Otón, Eva Pereiro, José J. Conesa, Francisco J. Chichón, Daniel Luque, Javier M. Rodríguez, Ana J. Pérez-Berná, Carlos Oscar S. Sorzano, Joanna Klukowska, Gabor T. Herman, Javier Vargas, Roberto Marabini, José L. Carrascosa, José M. Carazo
Published in: Scientific Reports, Issue 7, 2017, Page(s) 45808, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/srep45808

Solution NMR Structure of a Ligand/Hybrid-2-G-Quadruplex Complex Reveals Rearrangements that Affect Ligand Binding

Author(s): Julia Wirmer-Bartoschek, Lars Erik Bendel, Hendrik R. A. Jonker, J. Tassilo Grün, Francesco Papi, Carla Bazzicalupi, Luigi Messori, Paola Gratteri, Harald Schwalbe
Published in: Angewandte Chemie International Edition, 2017, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201702135

ATSAS 2.8 : a comprehensive data analysis suite for small-angle scattering from macromolecular solutions

Author(s): D. Franke, M. V. Petoukhov, P. V. Konarev, A. Panjkovich, A. Tuukkanen, H. D. T. Mertens, A. G. Kikhney, N. R. Hajizadeh, J. M. Franklin, C. M. Jeffries, D. I. Svergun
Published in: Journal of Applied Crystallography, Issue 50/4, 2017, ISSN 1600-5767
Publisher: IUCr/Wiley
DOI: 10.1107/S1600576717007786

Crabrolin, a natural antimicrobial peptide: structural properties

Author(s): Massimiliano Aschi, Argante Bozzi, Carla Luzi, Nadia Bouchemal, Marco Sette
Published in: Journal of Peptide Science, Issue June 5, 2017, ISSN 1075-2617
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/psc.3013

Mistakes in translation: Reflections on mechanism

Author(s): Yizhou Liu, Joshua S. Sharp, Duc H-T. Do, Richard A. Kahn, Harald Schwalbe, Florian Buhr, James H. Prestegard
Published in: PLOS ONE, Issue 12/6, 2017, Page(s) e0180566, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0180566

Structural insights into the molecular function of human [2Fe-2S] BOLA1-GRX5 and [2Fe-2S] BOLA3-GRX5 complexes

Author(s): Veronica Nasta, Andrea Giachetti, Simone Ciofi-Baffoni, Lucia Banci
Published in: Biochimica et Biophysica Acta (BBA) - General Subjects, Issue 1861/8, 2017, Page(s) 2119-2131, ISSN 0304-4165
Publisher: Elsevier BV
DOI: 10.1016/j.bbagen.2017.05.005

A Spring-Loaded Mechanism Governs the Clamp-like Dynamics of the Skp Chaperone

Author(s): Daniel A. Holdbrook, Björn M. Burmann, Roland G. Huber, Maxim V. Petoukhov, Dmitri I. Svergun, Sebastian Hiller, Peter J. Bond
Published in: Structure, Issue 25/7, 2017, Page(s) 1079-1088.e3, ISSN 0969-2126
Publisher: Cell Press
DOI: 10.1016/j.str.2017.05.018

Paramagnetic Properties of a Crystalline Iron–Sulfur Protein by Magic-Angle Spinning NMR Spectroscopy

Author(s): Andrea Bertarello, Tobias Schubeis, Carmelo Fuccio, Enrico Ravera, Marco Fragai, Giacomo Parigi, Lyndon Emsley, Guido Pintacuda, Claudio Luchinat
Published in: Inorganic Chemistry, Issue 56/11, 2017, Page(s) 6624-6629, ISSN 0020-1669
Publisher: American Chemical Society
DOI: 10.1021/acs.inorgchem.7b00674

Combining NMR and small angle X-ray scattering for the study of biomolecular structure and dynamics

Author(s): Haydyn D.T. Mertens, Dmitri I. Svergun
Published in: Archives of Biochemistry and Biophysics, Issue 628, 2017, Page(s) 33-41, ISSN 0003-9861
Publisher: Academic Press
DOI: 10.1016/j.abb.2017.05.005

Anthranilate phosphoribosyltransferase from the hyperthermophilic archaeon Thermococcus kodakarensis shows maximum activity with zinc and forms a unique dimeric structure

Author(s): Sumera Perveen, Naeem Rashid, Xiao-Feng Tang, Tadayuki Imanaka, Anastassios C. Papageorgiou
Published in: FEBS Open Bio, Issue 7/8, 2017, Page(s) 1217-1230, ISSN 2211-5463
Publisher: Elsevier BV
DOI: 10.1002/2211-5463.12264

A Novel Approach to Data Collection for Difficult Structures: Data Management for Large Numbers of Crystals with the BLEND Software

Author(s): Anastasia Mylona, Stephen Carr, Pierre Aller, Isabel Moraes, Richard Treisman, Gwyndaf Evans, James Foadi
Published in: Crystals, Issue 7/8, 2017, Page(s) 242, ISSN 2073-4352
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cryst7080242

Anamorsin/Ndor1 Complex Reduces [2Fe–2S]-MitoNEET via a Transient Protein–Protein Interaction

Author(s): Francesca Camponeschi, Simone Ciofi-Baffoni, Lucia Banci
Published in: Journal of the American Chemical Society, Issue 139/28, 2017, Page(s) 9479-9482, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.7b05003

An antimicrobial peptide that inhibits translation by trapping release factors on the ribosome

Author(s): Tanja Florin, Cristina Maracci, Michael Graf, Prajwal Karki, Dorota Klepacki, Otto Berninghausen, Roland Beckmann, Nora Vázquez-Laslop, Daniel N Wilson, Marina V Rodnina, Alexander S Mankin
Published in: Nature Structural & Molecular Biology, Issue 24/9, 2017, Page(s) 752-757, ISSN 1545-9993
Publisher: Nature Publishing Group
DOI: 10.1038/nsmb.3439

Lipid-like Peptides can Stabilize Integral Membrane Proteins for Biophysical and Structural Studies

Author(s): Katharina Veith, Maria Martinez Molledo, Yasser Almeida Hernandez, Inokentijs Josts, Julius Nitsche, Christian Löw, Henning Tidow
Published in: ChemBioChem, Issue 18/17, 2017, Page(s) 1735-1742, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201700235

Evaluation of 15N-detected H–N correlation experiments on increasingly large RNAs

Author(s): Robbin Schnieders, Christian Richter, Sven Warhaut, Vanessa de Jesus, Sara Keyhani, Elke Duchardt-Ferner, Heiko Keller, Jens Wöhnert, Lars T. Kuhn, Alexander L. Breeze, Wolfgang Bermel, Harald Schwalbe, Boris Fürtig
Published in: Journal of Biomolecular NMR, Issue 69/1, 2017, Page(s) 31-44, ISSN 0925-2738
Publisher: Kluwer Academic Publishers
DOI: 10.1007/s10858-017-0132-7

An effective introduction to structural crystallography using 1D Gaussian atoms

Author(s): Emily Smith, Gwyndaf Evans, James Foadi
Published in: European Journal of Physics, Issue 38/6, 2017, Page(s) 065501, ISSN 0143-0807
Publisher: Institute of Physics Publishing
DOI: 10.1088/1361-6404/aa8188

Saposin-Lipoprotein Scaffolds for Structure Determination of Membrane Transporters

Author(s): Joseph A. Lyons, Andreas Bøggild, Poul Nissen, Jens Frauenfeld
Published in: Methods in Enzymology, Issue 19/07/2017, 2017, Page(s) 85-99
Publisher: Elsevier
DOI: 10.1016/bs.mie.2017.06.035

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