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MEMBRANE PROTEINS OF ELECTROACTIVE BACTERIA PROBED AT LIPID LAYERS ONTO MODIFIED ELECTRODES

Objective

MELBA aims at developing an efficient and versatile electrochemical platform for probing the charge transfer properties, electronic or protonic, of membrane proteins extracted from Gram negative electroactive bacteria. This 2 years research project will be conducted at the Université de Rennes 1, UR1 France, including a six months Secondment at Universidade Nova de Lisboa, UNL Portugal. Carbon electrodes will be covalently modified with pH-responsive electrophores through surface modification techniques, WP1 Task1. Lipid layers will be deposited onto the modified electrodes to create an artificial membrane as a host for extracted membrane proteins, WP1 Task2. The experienced researcher Dr. Lebègue will learn protein expression, extraction, purification and characterization during the six months Secondment at UNL, WP2 Task3. Finally extracted proteins will be incorporated into the artificial membrane deposited onto the modified electrodes and their electronic and/or protonic charge transfer properties will be characterized, WP2 Task4.
The ambition of MELBA is to understand the coupling of extracellular electron transfer with proton transport in Gram (-) electroactive bacteria. The outcome of MELBA will be useful to optimize potential microbial electrochemical technologies based on electroactive bacteria and will be applied to the characterization of the charge transfer properties of any membrane protein. Within MELBA, 3 oral communications will be given at international conferences, 5 high impact papers will be published in the field of bioelectrochemistry and 5 formal public engagement actions have been scheduled. MELBA will allow Dr. Lebègue to develop and extend her expertise in electrochemistry, materials science and surface modification to microbiology and biochemistry. Dr. Lebègue will enter the field of microbial electrochemistry and acquire a unique and solid interdisciplinary profile, required to compete for permanent academic positions and grants in Europe.

Fields of science (EuroSciVoc)

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Topic(s)

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MSCA-IF-EF-ST - Standard EF

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Call for proposal

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(opens in new window) H2020-MSCA-IF-2016

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Coordinator

UNIVERSITE DE RENNES I
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 185 076,00
Address
RUE DU THABOR 2
35065 RENNES CEDEX
France

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Region
Bretagne Bretagne Ille-et-Vilaine
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 185 076,00
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