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Engineered microbial factories for CO2 exploitation in an integrated waste treatment platform

Deliverables

Social impact and public acceptance

This report will describe the main social implicationsbarriers of the deployment of the ENGICOIN technologies and describe how to address them in an effective manner

Environmental Impact (LCA & REACH)

This report will show the results of the LCE and REACH analyses on the ENGICOIN processes and materials

Communication material

Project communication material including visual identity and basic project rules for communication

Report on final dissemination workshop and training event

Report on the final EU level workshop and training with presentation of the project achievements to the general public policy makers main stakeholders and students

Dissemination plan update

Update of the awareness and dissemination plan outlining the target audiences, key messages, communication channels, implementation timing and role and responsibilities of partners

ENGICOIN business and exploitation plan

Detailed plan on the ENGICOIN business and exploitation strategy containing identification of project exploitable results market analysis exploitation purposes operational processes impact plan and measurable indicators

Final report on dissemination activities

Report on project dissemination activities media relations and outreach

Training seminars (1)

Development of training courses for students and professionals

Pre-normative and regulatory impacts

Some prenormative and regulatory implications of the ENGICOIN technologies will be discussed in this report

Dissemination plan

Awareness and dissemination plan outlining the target audiences, key messages, communication channels, implementation timing and role and responsibilities of partners

Training seminars (2)

Development of training courses for students and professionals

Project website

Publication of the ENGICOIN project public website (with information on the projects, partners profiles and feeds from relevant social networks) and internal repository for document sharing

Production & distribution of an educational material on digital support

Educational material on the final event to be distributed both via digital support USB memory to training participants and available for download from the project web page

Publications

Providing new insights on the biphasic lifestyle of the predatory bacterium Bdellovibrio bacteriovorus through genome-scale metabolic modeling.

Author(s): Cristina Herencias; Sergio Salgado-Briegas; María Auxiliadora Prieto; Juan Nogales
Published in: PLoS Computational Biology, Issue 1, 2020, ISSN 1553-734X
Publisher: Public Library of Science
DOI: 10.1371/journal.pcbi.1007646

When microbial biotechnology meets material engineering.

Author(s): Ana M. Hernández-Arriaga; Cristina Campano; Virginia Rivero-Buceta; M. Auxiliadora Prieto
Published in: Microbial Biotechnology, Issue 2, 2021, ISSN 1751-7907
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/1751-7915.13975

Computational Analysis of Dynamic Light Exposure of Unicellular Algal Cells in a Flat-Panel Photobioreactor to Support Light-Induced CO2 Bioprocess Development.

Author(s): Nicolo' Santi Vasile; Alessandro Cordara; Giulia Usai; Giulia Usai; Angela Re
Published in: Front Microbiol, Issue 2, 2021, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2021.639482

On the use of oxygenic photosynthesis for the sustainable production of commodity chemicals

Author(s): Adam A. Pérez, Que Chen, Hugo Pineda Hernández, Filipe Branco dos Santos, Klaas J. Hellingwerf
Published in: Physiologia Plantarum, Issue 166/1, 2019, Page(s) 413-427, ISSN 0031-9317
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/ppl.12946

Environmental impacts and limitations of third-generation biobutanol: Life cycle assessment of n-butanol produced by genetically engineered cyanobacteria

Author(s): Astrid Nilsson, Kiyan Shabestary, Miguel Brandão, Elton P. Hudson
Published in: Journal of Industrial Ecology, 2019, ISSN 1687-9708
Publisher: Hindawi Publishing Corporation
DOI: 10.1111/jiec.12843

InducibleCRISPR/Cas9AllowsforMultiplexedandRapidly SegregatedSingle-TargetGenomeEditinginSynechocystisSp.PCC 6803

Author(s): Ivana Cengic, Inés C.Canadas, Nigel P.Minton and Elton P.Hudson
Published in: acs synsthetic biology, 2022, ISSN 2161-5063
Publisher: American Chemical Society
DOI: 10.1021/acssynbio.2c00375

Slow Protein Turnover Explains Limited Protein-Level Response to Diurnal Transcriptional Oscillations in Cyanobacteria

Author(s): Jan Karlsen, Johannes Asplund-Samuelsson, Michael Jahn, Dóra Vitay and Elton P. Hudson
Published in: Frontiers in Microbiology, 2021, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2021.657379

Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes

Author(s): Lun Yao, Kiyan Shabestary, Sara M. Björk, Johannes Asplund-Samuelsson, Haakan N. Joensson, Michael Jahn & Elton P. Hudson
Published in: Nature Communications, 2020, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-020-15491-7

Response of the thylakoid proteome of Synechocystis sp. PCC 6803 to photohinibitory intensities of orange-red light

Author(s): Alessandro Cordara, Marcello Manfredi, Pascal van Alphen, Emilio Marengo, Raffaele Pirone, Guido Saracco, Filipe Branco dos Santos, Klaas J. Hellingwerf, Cristina Pagliano
Published in: Plant Physiology and Biochemistry, Issue 132, 2018, Page(s) 524-534, ISSN 0981-9428
Publisher: Elsevier BV
DOI: 10.1016/j.plaphy.2018.10.002

Analysis of the light intensity dependence of the growth of Synechocystis and of the light distribution in a photobioreactor energized by 635 nm light

Author(s): Alessandro Cordara, Angela Re, Cristina Pagliano, Pascal Van Alphen, Raffaele Pirone, Guido Saracco, Filipe Branco dos Santos, Klaas Hellingwerf, Nicolò Vasile
Published in: PeerJ, Issue 6, 2018, Page(s) e5256, ISSN 2167-8359
Publisher: PeerJ
DOI: 10.7717/peerj.5256

A Novel Redox-Sensing Histidine Kinase That Controls Carbon Catabolite Repression in Azoarcus sp. CIB

Author(s): J. Andrés Valderrama, Helena Gómez-Álvarez, Zaira Martín-Moldes, M. Álvaro Berbís, F. Javier Cañada, Gonzalo Durante-Rodríguez, Eduardo Díaz
Published in: mBio, Issue 10/2, 2019, ISSN 2150-7511
Publisher: ASM Journals
DOI: 10.1128/mBio.00059-19

Targeted Repression of Essential Genes To Arrest Growth and Increase Carbon Partitioning and Biofuel Titers in Cyanobacteria

Author(s): Kiyan Shabestary, Josefine Anfelt, Emil Ljungqvist, Michael Jahn, Lun Yao, Elton P. Hudson
Published in: ACS Synthetic Biology, Issue 7/7, 2018, Page(s) 1669-1675, ISSN 2161-5063
Publisher: American Chemical Society
DOI: 10.1021/acssynbio.8b00056

ArxA From Azoarcus sp. CIB, an Anaerobic Arsenite Oxidase From an Obligate Heterotrophic and Mesophilic Bacterium

Author(s): Gonzalo Durante-Rodríguez, Helga Fernández-Llamosas, Elena Alonso-Fernandes, María Nieves Fernández-Muñiz, Riansares Muñoz-Olivas, Eduardo Díaz, Manuel Carmona
Published in: Frontiers in Microbiology, Issue 10, 2019, ISSN 1664-302X
Publisher: Frontiers Media
DOI: 10.3389/fmicb.2019.01699

Further Insights into the Architecture of the PN Promoter That Controls the Expression of the bzd Genes in Azoarcus

Author(s): Durante-Rodríguez, Gutiérrez-del-Arroyo, Vélez, Díaz, Carmona
Published in: Genes, Issue 10/7, 2019, Page(s) 489, ISSN 2073-4425
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/genes10070489

Cycling between growth and production phases increases cyanobacteria bioproduction of lactate

Author(s): Kiyan Shabestary, Hugo Pineda Hernández, Rui Miao, Emil Ljungqvist, Olivia Hallman, Emil Sporre, Filipe Branco dos Santos, Elton P. Hudson
Published in: Metabolic Engineering, 2021, ISSN 1096-7176
Publisher: Academic Press
DOI: 10.1016/j.ymben.2021.09.010

Special Issue: Genetics of Biodegradation and Bioremediation

Author(s): Eduardo Santero, Eduardo Díaz
Published in: Genes, Issue 11/4, 2020, Page(s) 441, ISSN 2073-4425
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/genes11040441

Channeling Anabolic Side Products toward the Production of Nonessential Metabolites: Stable Malate Production in Synechocystis sp. PCC6803.

Author(s): Beatrice Battaglino; Beatrice Battaglino; Wei Du; Cristina Pagliano; Joeri A. Jongbloets; Angela Re; Guido Saracco; Filipe Branco dos Santos
Published in: ACS Synth Biol, Issue 2, 2021, ISSN 2161-5063
Publisher: American Chemical Society
DOI: 10.1021/acssynbio.1c00440

Synthetic metabolic pathways for conversion of CO2 into secreted short to medium chain hydrocarbons using cyanobacteria Metabolic engineering

Author(s): Ian S. Yunus, Josefine Anfelt, Emil Sporre b, Rui Miao, Elton P. Hudson, Patrik R. Jones
Published in: Metabolic Engineering, 2022, ISSN 1096-7176
Publisher: Academic Press
DOI: 10.1016/j.ymben.2022.01.017

Governing plant-centred eating at the urban scale in the UK: The Sustainable Food Cities network and the reframing of dietary biopower

Author(s): Carol Morris; Minna Kaljonen; Eleanor Hadley Kershaw
Published in: The geographical journal, Issue 2, 2021, ISSN 1475-4959
Publisher: Royal Geographical Society
DOI: 10.1111/geoj.12388

Providing new insights on the byphasic lifestyle of the predatory bacterium Bdellovibrio bacteriovorus through genome-scale metabolic modeling

Author(s): C Herencias; MA Prieto; J Nogales
Published in: PLoS Computational Biology, Issue 3, 2020, ISSN 1553-734X
Publisher: Public Library of Science
DOI: 10.1101/2020.01.13.904078

Exploiting Day- and Night-Time Metabolism of Synechocystis sp. PCC 6803 for Fitness-Coupled Fumarate Production around the Clock

Author(s): Wei Du, Joeri A. Jongbloets, Max Guillaume, Bram van de Putte, Beatrice Battaglino, Klaas J. Hellingwerf, Filipe Branco dos Santos
Published in: ACS Synthetic Biology, Issue 8/10, 2019, Page(s) 2263-2269, ISSN 2161-5063
Publisher: American Chemical Society
DOI: 10.1021/acssynbio.9b00289

Establishing Mixotrophic Growth of Cupriavidus necator H16 on CO2 and Volatile Fatty Acids. Fermentation

Author(s): Kamran Jawed,Victor Uhunoma Irorer, Rajesh Reddy Bommareddy,Nigel P. Minton and Katalin Kovács
Published in: Fermentation, 2022, ISSN 2311-5637
Publisher: Fermenation
DOI: 10.3390/fermentation8030125

Synthetic Biology in Cyanobacteria and Applications for Biotechnology

Author(s): Elton P. Hudson
Published in: 2021, ISBN 9783527347148
Publisher: wiley
DOI: 10.1002/9783527824908.ch5

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