CC-LEGO: robust protein blocks to build cages and layers

Información del proyecto

CC-LEGO

Identificador del acuerdo de subvención: 792305

DOI

10.3030/792305

Proyecto cerrado

Fecha de la firma de la CE 26 Febrero 2018

Fecha de inicio 1 Septiembre 2018

Fecha de finalización 31 Agosto 2022

Financiado con arreglo a

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Coste total

€ 250 774,20

Aportación de la UE

€ 250 774,20

Coordinado por

KEMIJSKI INSTITUT
Slovenia

Objetivo

The design of novel proteins offers unprecedented advantages for human health, such as improved vaccines, drug delivery and novel therapies (e.g. a flu inhibitor, treatment of Alzheimer’s). The state of the art of de novo self-assembling protein nanomaterials is based on the design of protein-protein interfaces. However, due to the complex interplay of numerous weak interactions at the interface the success rate of designed assemblies is currently below 10%. This proposal aims to increase the success rate of designing large protein assemblies, by using coiled-coils (CC) at the interface. CCs are some of the best understood protein motifs and can be designed with high success rates (>50%). A set of well-defined building blocks (CC-LEGOs) will be created by rigidly fusing CC bundles and natural oligomerization domains. Due to precise control of crossing angles enabled by the rigid fusion we expect that CC-LEGO structures will have the high design success rate and high solution homogeneity needed for further applications. A toolbox of CC-LEGO blocks will be designed and experimentally tested, capable of forming cages (tetrahedra, cubes, dodecahedra) as well as open 2D lattices.

The experienced researcher (ER) will gain new programming and wet lab skills for protein design through performing world-class research at one of the world’s top universities in a group of a world leader in this field. The expected high impact publications and networking opportunities, coupled with the acquired soft skills in project writing, management, leadership and communication will ensure ER’s competitiveness and enable a successful start of his independent career.
Another important impact of the project is the transfer the protein design know-how from the University of Washington to the Slovenian and regional research ecosystem, increasing its excellence. This will be supported by the beneficiary in Slovenia, where most of the wet-lab and computational infrastructure is already in place.

Ámbito científico (EuroSciVoc)

• ciencias médicas y de la salud › medicina básica › neurología › demencia › alzheimer
• ciencias naturales › ciencias biológicas › bioquímica › biomoléculas › proteínas
• ciencias médicas y de la salud › medicina básica › farmacología y farmacia › medicamento › vacuna
• ciencias naturales › ciencias biológicas › ecología › ecosistemas
• ingeniería y tecnología › nanotecnología › nanomateriales

Palabras clave

• de novo protein design
• coiled coils
• protein assemblies

Programa(s)

• H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions PROGRAMA PRINCIPAL
  
• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
  

Tema(s)

• MSCA-IF-2017 - Individual Fellowships
  

Régimen de financiación

MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

Convocatoria de propuestas

H2020-MSCA-IF-2017

Coordinador

KEMIJSKI INSTITUT

• Aportación neta de la UEn: € 250 774,20
• Dirección: HAJDRIHOVA 19 ; 1000 Ljubljana ; Eslovenia
• Región: Slovenija › Zahodna Slovenija › Osrednjeslovenska
• Tipo de actividad: Research Organisations
• Coste total: € 250 774,20

Socios (1)

Socio

UNIVERSITY OF WASHINGTON

Estados Unidos

• Aportación neta de la UEn: € 0,00
• Dirección: BROOKLYN AVENUE NE 4333 ; 98195 9472 Seattle Wa
• Tipo de actividad: Higher or Secondary Education Establishments
• Coste total: € 172 130,40