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Nanoscale Analytical Methods to gain insight into the Initiation of Short-Term Memory

Objective

-The aim of this project is to develop novel nano-bioanalytical methodologies to explore a molecular paradigm for the initiation of short-term memory (STM) based on exocytosis events. Despite the vital role in neural disorders such as Alzheimer’s disease, the molecular mechanisms of the initial step in STM formation is still mostly a mystery. Understanding STM initiation at the molecular level requires advanced analytical measurements at the level of attoliter vesicles and the synaptic cleft to monitor individual exocytosis events. This poses an extreme analytical challenge. “NAMISTMem” will develop new strategies for quantitative determination of the entire vesicle content (Objective 1), and will also provide a completely new direction of research that will measure cellular and vesicular changes in chemical endogenous factors (e.g. lipids and zinc) over time following an exocytosis event and will correlate these changes to the molecular initiation of STM (Objectives 2 and 3). These changes, which might alter the neurotransmission process, can be hypothesized to be the initial step that alters synaptic plasticity and commencement of STM. These studies will be developed based on state of the art analytical techniques including in-vivo Nanoelectrochemistry, Secondary Ion Mass Spectrometry (SIMS), NanoSIMS, and Helium Ion Microscopy on model nerve-like cells and simple animal nerve cells (Pheochromocytoma Cell and Drosophila’s brain, respectively).
Overall, the proposed methods will have immediate and broad impact in large fields of analytical and life science via pushing the small size limits of bioanalytical probes to achieve a better understanding of the neuron and STM; and will provide innovative analytical approaches for modern brain science discoveries.

Fields of science (EuroSciVoc)

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Programme(s)

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Topic(s)

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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MSCA-IF-EF-ST - Standard EF

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) H2020-MSCA-IF-2017

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Coordinator

GOETEBORGS UNIVERSITET
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 185 857,20
Address
VASAPARKEN
405 30 Goeteborg
Sweden

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Region
Södra Sverige Västsverige Västra Götalands län
Activity type
Higher or Secondary Education Establishments
Links
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 185 857,20
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