Repurposing small RNA from ciliates for genome editing: single-molecule study

Información del proyecto

MIGHTY_RNA

Identificador del acuerdo de subvención: 819299

DOI

10.3030/819299

Proyecto cerrado

Fecha de la firma de la CE 18 Diciembre 2018

Fecha de inicio 1 Mayo 2019

Fecha de finalización 30 Abril 2024

Financiado con arreglo a

• EXCELLENT SCIENCE - European Research Council (ERC)

Coste total

€ 1 792 850,00

Aportación de la UE

€ 1 792 850,00

Coordinado por

TECHNISCHE UNIVERSITEIT DELFT
Netherlands

Periodic Reporting for period 4 - MIGHTY_RNA (Repurposing small RNA from ciliates for genome editing: single-molecule study)

Período documentado: 2023-11-01 hasta 2024-04-30

Resumen del contexto y de los objetivos generales del proyecto

The “MIGHTY_RNA” project addressed the critical need for more precise and efficient genome editing techniques, which are essential for advancing the understanding and treatment of genetic disorders, cancers, and other diseases. Improved genome editing methods are vital for society as they hold the potential to revolutionize healthcare, enhance food security, and foster innovations in personalized medicine, ultimately improving the quality of life and driving economic growth. The project aimed to elucidate the molecular mechanisms of DNA modification and apply these insights to genome editing. Specifically, it sought to elucidate the functional modes of Argonaute-mediated target recognition by developing a new single-molecule technique named SPARXS and to expand the study to include other genome editing systems.

Trabajo realizado desde el comienzo del proyecto hasta el final del período abarcado por el informe y los principales resultados hasta la fecha

From the outset, the project focused on developing innovative methodologies and exploring new genome editing systems. The high throughput single-molecule fluorescence technique, SPARXS, was successfully developed, significantly enhancing the capacity for precise molecular studies. This breakthrough was well-documented and accepted by leading scientific journals, including Science. This research expanded to include the examination of other novel proteins, using single-molecule FRET to understand their functional aspects. These studies have been published in high-impact journals like Nature Communications, providing valuable insights into genome editing. Throughout the project, the findings were disseminated through seminars, international conferences, and publications, promoting knowledge transfer.

Avances que van más allá del estado de la técnica e impacto potencial esperado (incluida la repercusión socioeconómica y las implicaciones sociales más amplias del proyecto hasta la fecha)

The project has significantly advanced the state of the art in genome editing. The development of SPARXS represents a major breakthrough, enabling high-throughput and precise observations of molecular interactions, with wide-ranging applications in medical diagnostics and therapeutic development. The exploration has opened new avenues for genome editing research, providing new insights into novel proteins’ biophysics and potential applications. These advancements are expected to continue driving innovation in genome editing, positioning Europe to lead future research and foster collaborations. Ultimately, the project’s achievements contribute to a future where genetic diseases can be better understood and treated, improving health outcomes and quality of life globally.

Small RNA-directed DNA elimination