Self-organization of the cilium: the role of the tubulin code

Project Information

CiliaTubulinCode

Grant agreement ID: 819826

DOI

10.3030/819826

Project closed

EC signature date 6 February 2019

Start date 1 March 2019

End date 31 October 2024

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 986 406,00

EU contribution

€ 1 986 406,00

Coordinated by

FONDAZIONE HUMAN TECHNOPOLE
Italy

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (17)

An In-depth Guide to the Ultrastructural Expansion Microscopy (U-ExM) of Chlamydomonas reinhardtii.

Author(s): Klena N, Maltinti G, Batman U, Pigino G, Guichard P, Hamel V.
Published in: Bio-Protocols, 2023, ISSN 2331-8325
Publisher: The Authors; exclusive licensee Bio-protocol LLC
DOI: 10.21769/bioprotoc.4792

The structural basis of intraflagellar transport at a glance

Author(s): Mareike A. Jordan, Gaia Pigino
Published in: Journal of Cell Science, Issue 00219533, 2021, Page(s) jcs247163, ISSN 0021-9533
Publisher: The Company of Biologists Ltd.
DOI: 10.1242/jcs.247163

In situ architecture of the ciliary base reveals the stepwise assembly of intraflagellar transport trains.

Author(s): van den Hoek H, Klena N, Jordan MA, Alvarez Viar Gonzalo, Righetto RD, Schaffer M, Erdmann PS, Wan W, Geimer S, Plitzko JM, Baumeister W, Pigino Gaia, Hamel V, Guichard P, Engel BD.
Published in: Science, Issue 00368075, 2022, Page(s) 543-548, ISSN 0036-8075
Publisher: American Association for the Advancement of Science
DOI: 10.1126/science.abm6704

The molecular structure of mammalian primary cilia revealed by cryo-electron tomography

Author(s): Petra Kiesel, Gonzalo Alvarez Viar, Nikolai Tsoy, Riccardo Maraspini, Peter Gorilak, Vladimir Varga, Alf Honigmann, Gaia Pigino
Published in: Nature Structural & Molecular Biology, 2020, ISSN 1545-9993
Publisher: Nature Publishing Group
DOI: 10.1038/s41594-020-0507-4

Tubulin tyrosination/detyrosination regulate the affinity and sorting of intraflagellar transport trains on axonemal microtubule doublets

Author(s): Aditya Chhatre, Ludek Stepanek, Adrian Pascal Nievergelt, Gonzalo Alvarez Viar, Stefan Diez, Gaia Pigino
Published in: Nature Communications, Issue 16, 2025, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-025-56098-0

The molecular structure of IFT-A and IFT-B in anterograde intraflagellar transport trains

Author(s): Samuel E Lacey, Helen E Foster, Gaia Pigino
Published in: Nature Structural Molecular Biology, 2023, ISSN 1545-9993
Publisher: Nature Publishing Group
DOI: 10.1038/s41594-022-00905-5

Extensive structural rearrangement of intraflagellar transport trains underpins bidirectional cargo transport

Author(s): Samuel E. Lacey, Andrea Graziadei, Gaia Pigino
Published in: Cell, Issue 187, 2024, Page(s) 4621-4636.e18, ISSN 0092-8674
Publisher: Cell Press
DOI: 10.1016/j.cell.2024.06.041

Tubulin glycylation controls axonemal dynein activity, flagellar beat, and male fertility

Author(s): Sudarshan Gadadhar, Gonzalo Alvarez Viar, Jan Niklas Hansen, An Gong, Aleksandr Kostarev, Côme Ialy-Radio, Sophie Leboucher, Marjorie Whitfield, Ahmed Ziyyat, Aminata Touré, Luis Alvarez, Gaia Pigino, Carsten Janke
Published in: Science, Issue 371/6525, 2021, Page(s) all, ISSN 0036-8075
Publisher: American Association for the Advancement of Science
DOI: 10.1126/science.abd4914

Structural Biology of Cilia and Intraflagellar Transport

Author(s): Nikolai Klena, Gaia Pigino
Published in: Annual Review of Cell and Developmental Biology, 2022, ISSN 1081-0706
Publisher: Annual Reviews, Inc.
DOI: 10.1146/annurev-cellbio-120219-034238

Tubulin posttranslational modifications through the lens of new technologies

Author(s): Gonzalo Alvarez Viar, Gaia Pigino
Published in: Current Opinion in Cell Biology, Issue 88, 2024, Page(s) 102362, ISSN 0955-0674
Publisher: Elsevier BV
DOI: 10.1016/j.ceb.2024.102362

In vivo imaging shows continued association of several IFT-A, IFT-B and dynein complexes while IFT trains U-turn at the tip

Author(s): Jenna L. Wingfield, Betlehem Mekonnen, Ilaria Mengoni, Peiwei Liu,Mareike Jordan, Dennis Diener, Gaia Pigino , Karl Lechtreck
Published in: Journal of Cell Science, Issue 00219533, 2021, Page(s) jcs259010, ISSN 0021-9533
Publisher: The Company of Biologists Ltd.
DOI: 10.1242/jcs.259010

Protocol for precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas

Author(s): Adrian Pascal Nievergelt, Dennis Ray Diener, Aliona Bogdanova, Thomas Brown, Gaia Pigino
Published in: STAR Protocols, 2024, ISSN 2666-1667
Publisher: Elsevier
DOI: 10.1016/j.xpro.2023.102774

Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas

Author(s): Adrian Pascal Nievergelt, Dennis Ray Diener, Aliona Bogdanova, Thomas Brown, Gaia Pigino
Published in: Cell Reports Methods, 2023, ISSN 0346-251X
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.crmeth.2023.100562

Conversion of anterograde into retrograde trains is an intrinsic property of intraflagellar transport.

Author(s): Nievergelt Adrian, Zykov Ilia, Diener Diener, Chhatre Aditya, Buchholz TimOliver, Delling Markus, Diez Stefan, Jug Florian, Štěpánek Ludek, Pigino Gaia.
Published in: Current Biology, Issue 09609822, 2022, Page(s) 4071-4078, ISSN 0960-9822
Publisher: Cell Press
DOI: 10.1016/j.cub.2022.07.033

Structure, interaction and nervous connectivity of beta cell primary cilia

Author(s): Andreas Müller, Nikolai Klena, Song Pang, Leticia Elizabeth Galicia Garcia, Oleksandra Topcheva, Solange Aurrecoechea Duran, Davud Sulaymankhil, Monika Seliskar, Hassan Mziaut, Eyke Schöniger, Daniela Friedland, Nicole Kipke, Susanne Kretschmar, Carla Münster, Jürgen Weitz, Marius Distler, Thomas Kurth, Deborah Schmidt, Harald F. Hess, C. Shan Xu, Gaia Pigino, Michele Solimena
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-024-53348-5

Ccdc113/Ccdc96 complex, a novel regulator of ciliary beating that connects radial spoke 3 to dynein g and the nexin link

Author(s): Rafał Bazan, Adam Schröfel, Ewa Joachimiak, Martyna Poprzeczko, Gaia Pigino, Dorota Wloga
Published in: PLOS Genetics, Issue 17/3, 2021, Page(s) e1009388, ISSN 1553-7404
Publisher: Public Library of Science
DOI: 10.1371/journal.pgen.1009388

Protofilament-specific nanopatterns of tubulin post-translational modifications regulate the mechanics of ciliary beating

Author(s): Gonzalo Alvarez Viar, Nikolai Klena, Fabrizio Martino, Adrian Pascal Nievergelt, Davide Bolognini, Paola Capasso, Gaia Pigino
Published in: Current Biology, Issue 34, 2025, Page(s) 4464-4475.e9, ISSN 0960-9822
Publisher: Cell Press
DOI: 10.1016/j.cub.2024.08.021

Other (1)

The Tubulin Nano-Code: a protofilament-specific pattern of tubulin post-translational modifications regulates ciliary beating mechanics

Author(s): G. Alvarez Viar, N. Klena, F. Martino, A. Nievergelt, G. Pigino
Published in: BioRxiv, 2023
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2023.06.28.546853