Brain injury in the premature born infant: stem cell regeneration research network

Project Information

PREMSTEM

Grant agreement ID: 874721

DOI

10.3030/874721

Project closed

EC signature date 6 December 2019

Start date 1 January 2020

End date 31 December 2025

Funded under

• SOCIETAL CHALLENGES - Health, demographic change and well-being

Total cost

€ 10 708 285,00

EU contribution

€ 8 999 620,00

Coordinated by

INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE
France

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Documents, reports (2)

Interim report on content elements
Open Innovation activities: Co-Creation Sessions

Publications

Peer reviewed articles (37)

Umbilical cord-mesenchymal stem cells induce a memory phenotype in CD4+ T cells.

Author(s): Sengun E, Wolfs TGAM, van Bruggen VLE, van Cranenbroek B, Simonetti ER, Ophelders D, de Jonge MI, Joosten I, van der Molen RG.
Published in: Frontiers Immunology, Issue Volume 14 - 2023, 2023, ISSN 1664-3224
Publisher: Frontiers
DOI: 10.3389/fimmu.2023.1128359

A Comprehensive Review of the Pathophysiology of Neonatal Stroke and a Critique of Current and Future Therapeutic Strategies

Author(s): Victor Mondal, Emily Ross-Munro, Gayathri K. Balasuriya, Ritu Kumari, Md. Munnaf Hossen, Mohammed Ageeli, Kate Firipis, David R. Nisbet, Glenn F. King, Richard J. Williams, Pierre Gressens, Jeanie L. Y. Cheong, Flora Y. Wong, David W. Walker, Mary Tolcos, Bobbi Fleiss
Published in: Cells, Issue 14, 2025, Page(s) 910, ISSN 2073-4409
Publisher: MDPI AG
DOI: 10.3390/cells14120910

A unique cerebellar pattern of microglia activation in a mouse model of encephalopathy of prematurity

Author(s): Klein L.; Van Steenwinckel J.; Fleiss B.; Scheuer T.; Bührer C.; Faivre V.; Bokobza C.; Lemoine S.; Blugeon C..; Schwendimann L.; Csaba Z.; Vousden D.; Lerch J.; Vernon A.; Gressens P.; Schmitz T
Published in: Glia, 2022, Page(s) 1699-1719, ISSN 1098-1136
Publisher: Wiley
DOI: 10.1002/glia.24190

Gold Mesoporous Silica-Coated Nanoparticles for Quantifying and Qualifying Mesenchymal Stem Cell Distribution; a Proof-of-Concept Study in Large Animals

Author(s): Lotte C. C. Smeets, Ezgi Sengun, Chloe Trayford, Bram van Cranenbroek, Marien I. de Jonge, Katiuscia Dallaglio, Matthias C. Hütten, Mark Schoberer, Daan R. M. G. Ophelders, Tim G. A. M. Wolfs, Renate G. van der Molen, Sabine van Rijt
Published in: ACS Applied Bio Materials, Issue 8, 2025, Page(s) 1511-1523, ISSN 2576-6422
Publisher: American Chemical Society (ACS)
DOI: 10.1021/acsabm.4c01714

Preterm birth: A neuroinflammatory origin for metabolic diseases?

Author(s): Sihao Diao, Chao Chen, Alexandre Benani, Christophe Magnan, Juliette Van Steenwinckel, Pierre Gressens, Céline Cruciani-Guglielmacci, Alice Jacquens, Cindy Bokobza
Published in: Brain, Behavior, & Immunity - Health, Issue 37, 2024, Page(s) 100745, ISSN 2666-3546
Publisher: ELSEVIER
DOI: 10.1016/j.bbih.2024.100745

Cortical Gray Matter Injury in Encephalopathy of Prematurity: Link to Neurodevelopmental Disorders

Author(s): Bobbi Fleiss, Pierre Gressens, Helen B. Stolp
Published in: Frontiers in Neurology, Issue 11, 2020, Page(s) 1-21, ISSN 1664-2295
Publisher: Frontiers Research Foundation
DOI: 10.3389/fneur.2020.00575

Therapeutic potential of stem cells for preterm infant brain damage: Can we move from the heterogeneity of preclinical and clinical studies to established therapeutics?

Author(s): Sofia Passera, Marta Boccazzi, Cindy Bokobza, Valerie Faivre, Fabio Mosca, Juliette Van Steenwinckel, Monica Fumagalli, Pierre Gressens, Bobbi Fleiss
Published in: Biochemical Pharmacology, Issue 186, 2021, Page(s) 114461, ISSN 0006-2952
Publisher: Elsevier BV
DOI: 10.1016/j.bcp.2021.114461

Human Umbilical Cord-Mesenchymal Stem Cells Promote Extracellular Matrix Remodeling in Microglia

Author(s): Marta Tiffany Lombardo, Martina Gabrielli, Florence Julien-Marsollier, Valérie Faivre, Tifenn Le Charpentier, Cindy Bokobza, Deborah D’Aliberti, Nicola Pelizzi, Camilla Halimi, Silvia Spinelli, Juliette Van Steenwinckel, Elisabetta A. M. Verderio, Pierre Gressens, Rocco Piazza, Claudia Verderio
Published in: Cells, Issue 13, 2025, Page(s) 1665, ISSN 2073-4409
Publisher: MDPI AG
DOI: 10.3390/cells13191665

miR-146b Protects the Perinatal Brain against Microglia-Induced Hypomyelination

Author(s): Bokobza C.; Joshi P.; Schang AL.; Csaba Z.; Faivre V.; Montané A.; Galland A.; Benmamar-Badel A.; Bosher E.; Lebon S.; Schwendimann L.; Mani S.; Dournaud P.; Besson V.; Fleiss B.; Gressens P.; Van Steenwinckel J.
Published in: Annals of Neurology, 2021, Page(s) 48-65, ISSN 1531-8249
Publisher: Wiley
DOI: 10.1002/ana.26263

Induction of Mitochondrial Fragmentation and Mitophagy after Neonatal Hypoxia–Ischemia

Author(s): Syam Nair, Anna-Lena Leverin, Eridan Rocha-Ferreira, Kristina S. Sobotka, Claire Thornton, Carina Mallard, Henrik Hagberg
Published in: Cells, Issue 11, 2025, Page(s) 1193, ISSN 2073-4409
Publisher: MDPI AG
DOI: 10.3390/cells11071193

Key roles of glial cells in the encephalopathy of prematurity

Author(s): Juliette Van Steenwinckel, Cindy Bokobza, Mireille Laforge, Isabelle K. Shearer, Veronique E. Miron, Rejane Rua, Samantha M. Matta, Elisa L. Hill‐Yardin, Bobbi Fleiss, Pierre Gressens
Published in: Glia, Issue 72, 2024, Page(s) 475-503, ISSN 0894-1491
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/glia.24474

A Neonatal Rodent Model of Retroorbital Vein Injection

Author(s): Eridan Rocha-Ferreira1, Syam Nair1, Owen Herrock1, Erik Axel Andersson2, Carl Joakim Ek2, Carina Mallard2, Henrik Hagberg1
Published in: Jove journal, Issue JOVE, 2024, Page(s) 1-8, ISSN 1940-087X
Publisher: MYJoVE Corporation
DOI: 10.3791/65386

Timed fetal inflammation and postnatal hypoxia cause cortical white matter injury, interneuron imbalances, and behavioral deficits in a double-hit rat model of encephalopathy of prematurity

Author(s): M.J.V. Brandt, C.M. Kosmeijer, E.J.M. Achterberg, C.G.M. de Theije, C.H.A. Nijboer
Published in: Brain, Behavior, & Immunity - Health, 2024, ISSN 2666-3546
Publisher: Elsevier Inc.
DOI: 10.1016/j.bbih.2024.100817

The immune-inflammatory response of oligodendrocytes in a murine model of preterm white matter injury: the role of TLR3 activation

Author(s): Marta Boccazzi, Juliette Van Steenwinckel, Anne-Laure Schang, Valérie Faivre, Tifenn Le Charpentier, Cindy Bokobza, Zsolt Csaba, Claudia Verderio, Marta Fumagalli, Shyamala Mani, Pierre Gressens
Published in: Cell Death & Disease, Issue 12/2, 2021, Page(s) 1-12, ISSN 2041-4889
Publisher: Nature Publishing Group
DOI: 10.1038/s41419-021-03446-9

Mesenchymal Stromal Cell-Derived Extracellular Vesicles Reduce Neuroinflammation, Promote Neural Cell Proliferation and Improve Oligodendrocyte Maturation in Neonatal Hypoxic-Ischemic Brain Injury

Author(s): Nicole Kaminski, Christian Köster, Yanis Mouloud, Verena Börger, Ursula Felderhoff-Müser, Ivo Bendix, Bernd Giebel, Josephine Herz
Published in: Frontiers in Cellular Neuroscience, Issue 14, 2020, ISSN 1662-5102
Publisher: Frontiers Research Foundation
DOI: 10.3389/fncel.2020.601176

The multiple faces of extracellular vesicles released by microglia: Where are we 10 years after?

Author(s): Martina Gabrielli, Stefano Raffaele, Marta Fumagalli, Claudia Verderio
Published in: Frontiers in Cellular Neuroscience, Issue 16, 2023, ISSN 1662-5102
Publisher: Frontiers Research Foundation
DOI: 10.3389/fncel.2022.984690

High sensitivity mapping of brain-wide functional networks in awake mice using simultaneous multi-slice fUS imaging

Author(s): Adrien Bertolo, Jeremy Ferrier, Silvia Cazzanelli, Samuel Diebolt, Mickael Tanter, Sophie Pezet, Mathieu Pernot, Bruno-Félix Osmanski, Thomas Deffieux
Published in: Imaging Neuroscience, Issue 1, 2023, Page(s) 1-18, ISSN 2837-6056
Publisher: Imaging Neuroscience
DOI: 10.1162/imag_a_00030

The Impact of Mouse Preterm Birth Induction by RU-486 on Microglial Activation and Subsequent Hypomyelination

Author(s): Morin C.; Guenoun D.; Faivre V.; Sautet I.; Schwendimann L.; Young-Ten P.; Gressens P.; Van Steenwinckel J.; Bokobza C.
Published in: IJMS, 2022, Page(s) 4867, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms23094867

G protein-coupled receptor 17 is regulated by WNT pathway during oligodendrocyte precursor cell differentiation

Author(s): Marta Boccazzi, Giulia Macchiarulo, Sophie Lebon, Justyna Janowska, Tifenn Le Charpentier, Valérie Faivre, Jennifer Hua, Davide Marangon, Davide Lecca, Marta Fumagalli, Shyamala Mani, Maria P. Abbracchio, Pierre Gressens, Anne-Laure Schang, Juliette Van Steenwinckel
Published in: Neurobiology of Disease, Issue 187, 2024, Page(s) 106315, ISSN 0969-9961
Publisher: Academic Press
DOI: 10.1016/j.nbd.2023.106315

Microglia-Mediated Neurodegeneration in Perinatal Brain Injuries

Author(s): Bobbi Fleiss, Juliette Van Steenwinckel, Cindy Bokobza, Isabelle K. Shearer, Emily Ross-Munro, Pierre Gressens
Published in: Biomolecules, Issue 11/1, 2021, Page(s) 99, ISSN 2218-273X
Publisher: MDPI
DOI: 10.3390/biom11010099

A Model of Germinal Matrix Hemorrhage in Preterm Rat Pups

Author(s): Masako Jinnai, Gabriella Koning, Gagandeep Singh-Mallah, Andrea Jonsdotter, Anna-Lena Leverin, Pernilla Svedin, Syam Nair, Satoru Takeda, Xiaoyang Wang, Carina Mallard, Carl Joakim Ek, Eridan Rocha-Ferreira, Henrik Hagberg
Published in: Frontiers in Cellular Neuroscience, Issue 14, 2020, ISSN 1662-5102
Publisher: Frontiers Research Foundation
DOI: 10.3389/fncel.2020.535320

C-section and systemic inflammation synergize to disrupt the neonatal gut microbiota and brain development in a model of prematurity

Author(s): Cécile Morin, Flora Faure, Julie Mollet, David Guenoun, Ariane Heydari-Olya, Irvin Sautet, Sihao Diao, Valérie Faivre, Julien Pansiot, Lara Tabet, Jennifer Hua, Leslie Schwendimann, Amazigh Mokhtari, Rebeca Martin-Rosique, Sead Chadi, Mireille Laforge, Charlie Demené, Andrée Delahaye-Duriez, Rochellys Diaz-Heijtz, Bobbi Fleiss, Boris Matrot, Sandrine Auger, Mickael Tanter, Juliette Van Steenwi
Published in: Brain, Behavior, and Immunity, Issue 123, 2025, Page(s) 824-837, ISSN 0889-1591
Publisher: Academic Press
DOI: 10.1016/j.bbi.2024.10.023

Preterm Brain Injury, Antenatal Triggers, and Therapeutics: Timing Is Key

Author(s): Daan R.M.G. Ophelders, Ruth Gussenhoven, Luise Klein, Reint K. Jellema, Rob J.J. Westerlaken, Matthias C. Hütten, Jeroen Vermeulen, Guido Wassink, Alistair J. Gunn, Tim G.A.M. Wolfs
Published in: Cells, Issue 9/8, 2020, Page(s) 1871, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/cells9081871

Extracellular Vesicle-Mediated Neuron–Glia Communications in the Central Nervous System

Author(s): Tsuneya Ikezu, Yongjie Yang, Claudia Verderio, Eva-Maria Krämer-Albers
Published in: The Journal of Neuroscience, Issue 44, 2024, Page(s) e1170242024, ISSN 0270-6474
Publisher: Society for Neuroscience
DOI: 10.1523/jneurosci.1170-24.2024

Neuroprotection offered by mesenchymal stem cells in perinatal brain injury: Role of mitochondria, inflammation, and reactive oxygen species

Author(s): Syam Nair, Eridan Rocha‐Ferreira, Bobbi Fleiss, Cora H Nijboer, Pierre Gressens, Carina Mallard, Henrik Hagberg
Published in: Journal of Neurochemistry, Issue 158/1, 2021, Page(s) 59-73, ISSN 0022-3042
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/jnc.15267

Prenatal inflammation exacerbates hyperoxia-induced neonatal brain injury

Author(s): Meray Serdar, Kay-Anja Walther, Markus Gallert, Karina Kempe, Stefanie Obst, Nicole Labusek, Ralf Herrmann, Josephine Herz, Ursula Felderhoff-Müser, Ivo Bendix
Published in: Journal of Neuroinflammation, Issue 22, 2025, ISSN 1742-2094
Publisher: BioMed Central
DOI: 10.1186/s12974-025-03389-4

Functional ultrasound (fUS) detects mild cerebral alterations using canonical correlation analysis denoising and dynamic functional connectivity analysis

Author(s): Flora Faure, Cindy Bokobza, David Guenoun, Juliette Van Steenwinckel, Pierre Gressens, Charlie Demené
Published in: Imaging Neuroscience, Issue 3, 2025, ISSN 2837-6056
Publisher: MIT Press
DOI: 10.1162/imag.a.128

Magnetic Isolation of Microglial Cells from Neonate Mouse for Primary Cell Cultures

Author(s): Cindy Bokobza, Alice Jacquens, Manuela Zinni, Valérie Faivre, Jennifer Hua, David Guenoun, Caroline Userovici, Shyamala Mani, Vincent Degos, Pierre Gressens, Juliette Van Steenwinckel
Published in: Journal of Visualized Experiments, 2025, ISSN 1940-087X
Publisher: MYJoVE Corporation
DOI: 10.3791/62964

Organ development in growth‐restricted fetuses in the reduced uterine perfusion pressure rat model: A vascular approach of brain, heart, and kidney

Author(s): J. Alhama‐Riba, C. M. van Kammen, K. T. Nijholt, D. Viveen, K. Amarouchi, D. Shasha, M. M. Krebber, F. E. Hoebeek, A. T. Lely, C. H. A. Nijboer, F. Terstappen
Published in: Physiological Reports, Issue 13, 2025, ISSN 2051-817X
Publisher: Wiley
DOI: 10.14814/phy2.70244

A systematic scoring system to optimise the testing of neurotherapeutics in models of perinatal brain injury, with an applied case study of human umbilical-cord MSC

Author(s): Cindy Bokobza, Clémence Réda, Syam Nair, David Guenoun, Eridan Rocha-Ferreira, Valérie Faivre, Tifenn Le Charpentier, Cora Nijboer, Caroline de Theije, Sophie Lebon, Joakim Ek, Mohamed Gaeth Hafez, Leslie Schwendimann, Médine Benchouaia, Sophie Lemoine, Giorgia Volpi, Katiuscia Dallaglio, Nicola Pelizzi, Bobbi Fleiss, Juliette Van Steenwinckel, Andrée Delahaye-Duriez, Henrik Hagberg, Pierre G
Published in: Journal of Neuroinflammation, Issue 23, 2026, ISSN 1742-2094
Publisher: BioMed Central
DOI: 10.1186/s12974-025-03593-2

A Novel Model for Simultaneous Evaluation of Hyperoxia-Mediated Brain and Lung Injury in Neonatal Rats

Author(s): Stefanie Obst, Meray Serdar, Josephine Herz, Karina Kempe, Meriem Assili, Mandana Rizazad, Dharmesh Hirani, Miguel A. Alejandre Alcazar, Stefanie Endesfelder, Marius A. Möbius, Mario Rüdiger, Ursula Felderhoff-Müser, Ivo Bendix
Published in: Cells, Issue 14, 2025, Page(s) 443, ISSN 2073-4409
Publisher: MDPI AG
DOI: 10.3390/cells14060443

Microglial Depletion, a New Tool in Neuroinflammatory Disorders: Comparison of Pharmacological Inhibitors of the <scp>CSF</scp>‐<scp>1R</scp>

Author(s): David Guenoun, Nathan Blaise, Alexandre Sellam, Julie Roupret‐Serzec, Alice Jacquens, Juliette Van Steenwinckel, Pierre Gressens, Cindy Bokobza
Published in: Glia, Issue 73, 2025, Page(s) 686-700, ISSN 0894-1491
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/glia.24664

Non-invasive characterization of pericyte dysfunction in mouse brain using functional ultrasound localization microscopy

Author(s): Jérémy H. Thalgott, Nicolas Zucker, Thomas Deffieux, Marit S. Koopman, Alexandre Dizeux, Cristina M. Avramut, Roman I. Koning, Hans-Jurgen Mager, Ton J. Rabelink, Mickaël Tanter, Franck Lebrin
Published in: Nature Biomedical Engineering, Issue 10, 2026, Page(s) 214-230, ISSN 2157-846X
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/s41551-025-01465-x

Targeting the brain 5-HT7 receptor to prevent hypomyelination in a rodent model of perinatal white matter injuries

Author(s): Cindy Bokobza, Alice Jacquens, David Guenoun, Blandine Bianco, Anne Galland, Maxime Pispisa, Alexandra Cruz, Manuela Zinni, Valérie Faivre, Anne Roumier, Sophie Lebon, Tania Vitalis, Zsolt Csaba, Tifenn Le Charpentier, Leslie Schwendimann, Pierrette Young-Ten, Vincent Degos, Patricia Monteiro, Pascal Dournaud, Pierre Gressens, Juliette Van Steenwinckel
Published in: Journal of Neural Transmission, Issue 130, 2023, Page(s) 281-297, ISSN 0300-9564
Publisher: Springer Verlag
DOI: 10.1007/s00702-022-02556-8

Hypothermia combined with extracellular vesicles from clonally expanded immortalized mesenchymal stromal cells improves neurodevelopmental impairment in neonatal hypoxic-ischemic brain injury

Author(s): Nicole Labusek 1, Parnian Ghari 1, Yanis Mouloud 2, Christian Köster 1, Eva Diesterbeck 1, Martin Hadamitzky 3, Ursula Felderhoff-Müser 1, Ivo Bendix 1, Bernd Giebel 2, Josephine Herz 4
Published in: National Library of Medicine, 2023, Page(s) 20(1):280, ISSN 1742-2094
Publisher: BioMed Central
DOI: 10.1186/s12974-023-02961-0

Quantification of brain-wide vascular resistivity via ultrafast Doppler in human neonates helps early detection of white matter injury

Author(s): Flora Faure, Jérôme Baranger, Marianne Alison, Béatrice Boutillier, Alice Frérot, Chung Lim, Grégory Planchette, Mickael Prigent, Mickaël Tanter, Olivier Baud, Valérie Biran, Charlie Demené
Published in: Journal of Cerebral Blood Flow &amp; Metabolism, 2024, ISSN 0271-678X
Publisher: Lippincott Williams & Wilkins Ltd.
DOI: 10.1177/0271678x241232197

Mesenchymal stem cells suppress NF-κB and ERK signalling while enhancing chemotaxis in CD4+ T cells

Author(s): Ezgi Sengun, Janeri Fröberg, Xuehui He, Marc Eleveld, Ruben L. Smeets, Hans J. P. M. Koenen, Katja Möller-Hackbarth, Tim G. A. M. Wolfs, Daan R. M. G. Ophelders, Martijn A. Huynen, Marien I. de Jonge, Renate G. van der Molen
Published in: Scientific Reports, Issue 15, 2025, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-025-14373-6

Book chapters (1)

Role of Microglial Modulation in Therapies for Perinatal Brain Injuries Leading to Neurodevelopmental Disorders

Author(s): Bobbi Fleiss, Pierre Gressens
Published in: Advances in Neurobiology, Microglia, 2024, Page(s) 591-606
Publisher: Springer International Publishing
DOI: 10.1007/978-3-031-55529-9_33