ADDovenom: Novel Snakebite Therapy Platform of Unparalleled Efficacy, Safety and Affordability

Informations projet

ADDovenom

N° de convention de subvention: 899670

DOI

10.3030/899670

Projet clôturé

Date de signature de la CE 26 Mai 2020

Date de début 1 Octobre 2020

Date de fin 31 Mars 2025

Financé au titre de

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coût total

€ 3 675 558,75

Contribution de l’UE

€ 3 675 558,75

Coordonné par

UNIVERSITY OF BRISTOL
United Kingdom

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Livrables

Documents, reports (15)

List of protein / peptide components for 8 African snake venoms and their relative abundance identified by MS

List of protein peptide components for 8 African snake venoms and their relative abundance identified by MS

White paper 1
List of identified toxins and their predicted pharmacological activity in the venoms (8 snakes)
List of sequences, PTMs, disulphide bridges and isoelectric points of the venom components (2 snakes)

List of sequences PTMs disulphide bridges and isoelectric points of the venom components 2 snakes

Pharmokinetic characterisation of ADDomer/ADDobodies in mice
Validated protocols for the production of ADDobodies in high-throughput and milligram amounts

Validated protocols for the production of ADDobodies in highthroughput and milligram amounts

Determination of venom toxicity (LD50) for each snake species

Determination of venom toxicity LD50 for each snake species

In process assays for ADDomers
Demonstration of safety of ADDomer/ADDobodies in mice
List of sequences, PTMs, disulphide bridges and isoelectric points of the venom components (8 snakes)
List of protein / peptide components for 2 African snake venoms and their relative abundance identified by MS

List of protein peptide components for 2 African snake venoms and their relative abundance identified by MS

Validated protocols for the production of toxins/ epitope strings

Validated protocols for the production of toxins epitope strings

In vitro functional ability of ADDomers/ADDobodies to neutralise toxin-function
List of identified toxins and their predicted pharmacological activity in the venoms (2 snakes)

List of identified toxins and their predicted pharmacological activity in the venoms 2 snakes

Determination of ADDomers/ADDobodies to neutralize venom-induced lethality using in vivo ED50 assays

Websites, patent fillings, videos etc. (2)

First version of the website
Toxin database

Open Research Data Pilot (1)

Data management plan

Publications

Peer reviewed articles (9)

Virus-like particles displaying conserved toxin epitopes stimulate polyspecific, murine antibody responses capable of snake venom recognition

Auteurs: Stefanie K. Menzies, Charlotte A. Dawson, Edouard Crittenden, Rebecca J. Edge, Steven R. Hall, Jaffer Alsolaiss, Mark C. Wilkinson, Nicholas R. Casewell, Robert A. Harrison, Stuart Ainsworth
Publié dans: Scientific Reports, Numéro 12, 2022, ISSN 2045-2322
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-022-13376-x

Dual Proteomics Strategies to Dissect and Quantify the Components of Nine Medically Important African Snake Venoms

Auteurs: Damien Redureau, Fernanda Gobbi Amorim, Thomas Crasset, Imre Berger, Christiane Schaffitzel, Stefanie Kate Menzies, Nicholas R. Casewell, Loïc Quinton
Publié dans: Toxins, Numéro 17, 2025, Page(s) 243, ISSN 2072-6651
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins17050243

Virus-like particles displaying conserved toxin epitopes stimulate polyspecific, murine antibody responses capable of snake venom recognition

Auteurs: Stefanie K. Menzies; Charlotte A. Dawson; Edouard Crittenden; Rebecca J. Edge; Steven R. Hall; Jaffer Alsolaiss; Mark C. Wilkinson; Nicholas R. Casewell; Robert A. Harrison; Stuart Ainsworth
Publié dans: Scientific Reports, 2022, ISSN 0028-0836
Éditeur: Nature Publishing Group
DOI: 10.1038/s41598-022-13376-x

De novo designed proteins neutralize lethal snake venom toxins

Auteurs: Susana Vázquez Torres, Melisa Benard Valle, Stephen P. Mackessy, Stefanie K. Menzies, Nicholas R. Casewell, Shirin Ahmadi, Nick J. Burlet, Edin Muratspahić, Isaac Sappington, Max D. Overath, Esperanza Rivera-de-Torre, Jann Ledergerber, Andreas H. Laustsen, Kim Boddum, Asim K. Bera, Alex Kang, Evans Brackenbrough, Iara A. Cardoso, Edouard P. Crittenden, Rebecca J. Edge, Justin Decarreau, Robert J
Publié dans: Nature, Numéro 639, 2025, Page(s) 225-231, ISSN 0028-0836
Éditeur: Nature Publishing Group
DOI: 10.1038/s41586-024-08393-x

Engineering the ADDobody protein scaffold for generation of high-avidity ADDomer super-binders

Auteurs: Dora Buzas, Huan Sun, Christine Toelzer, Sathish K. N. Yadav, Ufuk Borucu, Gunjan Gautam, Kapil Gupta, Josh Bufton, Julien Capin, Richard B. Sessions, Frederic Garzoni, Imre Berger, Christiane Schaffitzel
Publié dans: Structure, 2023, ISSN 0969-2126
Éditeur: Cell Press
DOI: 10.1101/2023.09.09.556966

Next-Generation Sequencing for Venomics: Application of Multi-Enzymatic Limited Digestion for Inventorying the Snake Venom Arsenal

Auteurs: Fernanda Gobbi Amorim, Damien Redureau, Thomas Crasset, Lou Freuville, Dominique Baiwir, Stefanie K. Menzies, Nicholas R. Casewell, Loïc Quinton
Publié dans: Toxins, Numéro 15(6), 2023, Page(s) 357, ISSN 2072-6651
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins15060357

Preclinical evaluation of the neutralising efficacy of three antivenoms against the venoms of the recently taxonomically partitioned E. ocellatus and E. romani

Auteurs: Rebecca J. Edge, Amy E. Marriott, Molly Keen, Tiffany Xie, Edouard P. Crittenden, Charlotte A. Dawson, Mark C. Wilkinson, Wolfgang Wüster, Nicholas R. Casewell, Stuart Ainsworth, Stefanie K. Menzies
Publié dans: PLOS Neglected Tropical Diseases, Numéro 19, 2025, Page(s) e0013371, ISSN 1935-2735
Éditeur: Public Library of Science (PLoS)
DOI: 10.1371/journal.pntd.0013371

In vitro generated antibodies guide thermostable ADDomer nanoparticle design for nasal vaccination and passive immunization against SARS-CoV-2

Auteurs: Dora Buzas, Adrian H Bunzel, Oskar Staufer, Emily J Milodowski, Grace L Edmunds, Joshua C Bufton, Beatriz V Vidana Mateo, Sathish K N Yadav, Kapil Gupta, Charlotte Fletcher, Maia K Williamson, Alexandra Harrison, Ufuk Borucu, Julien Capin, Ore Francis, Georgia Balchin, Sophie Hall, Mirella V Vega, Fabien Durbesson, Srikanth Lingappa, Renaud Vincentelli, Joe Roe, Linda Wooldridge, Rachel Burt, Ross
Publié dans: Antibody Therapeutics, Numéro tbad024, 2023, ISSN 2516-4236
Éditeur: Oxford Academic
DOI: 10.1093/abt/tbad024

ADDovenom: Thermostable Protein-Based ADDomer Nanoparticles as New Therapeutics for Snakebite Envenoming

Auteurs: Stefanie K. Menzies, Raquel A. Garcia, Fernanda Gobbi Amorim, Iara Aimê Cardoso,Camille Abada, Thomas Crasset, Rebecca J. Edge, Sophie Hall, Damien Redureau, Richard Stenner, Johara Stringari, António Roldão, Paula M.vAlves, Robert A. Harrison, Renaud Vincentelli, Huan Sun, Imre Berger, Loïc Quinton, Nicholas R. Casewell, Christiane Schaffitzel
Publié dans: Toxins, Numéro 15(12), 2023, Page(s) 673, ISSN 2072-6651
Éditeur: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins15120673