ADDovenom: Novel Snakebite Therapy Platform of Unparalleled Efficacy, Safety and Affordability

Información del proyecto

ADDovenom

Identificador del acuerdo de subvención: 899670

DOI

10.3030/899670

Proyecto cerrado

Fecha de la firma de la CE 26 Mayo 2020

Fecha de inicio 1 Octubre 2020

Fecha de finalización 31 Marzo 2025

Financiado con arreglo a

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coste total

€ 3 675 558,75

Aportación de la UE

€ 3 675 558,75

Coordinado por

UNIVERSITY OF BRISTOL
United Kingdom

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Documents, reports (15)

List of protein / peptide components for 8 African snake venoms and their relative abundance identified by MS

List of protein peptide components for 8 African snake venoms and their relative abundance identified by MS

White paper 1
List of identified toxins and their predicted pharmacological activity in the venoms (8 snakes)
List of sequences, PTMs, disulphide bridges and isoelectric points of the venom components (2 snakes)

List of sequences PTMs disulphide bridges and isoelectric points of the venom components 2 snakes

Pharmokinetic characterisation of ADDomer/ADDobodies in mice
Validated protocols for the production of ADDobodies in high-throughput and milligram amounts

Validated protocols for the production of ADDobodies in highthroughput and milligram amounts

Determination of venom toxicity (LD50) for each snake species

Determination of venom toxicity LD50 for each snake species

In process assays for ADDomers
Demonstration of safety of ADDomer/ADDobodies in mice
List of sequences, PTMs, disulphide bridges and isoelectric points of the venom components (8 snakes)
List of protein / peptide components for 2 African snake venoms and their relative abundance identified by MS

List of protein peptide components for 2 African snake venoms and their relative abundance identified by MS

Validated protocols for the production of toxins/ epitope strings

Validated protocols for the production of toxins epitope strings

In vitro functional ability of ADDomers/ADDobodies to neutralise toxin-function
List of identified toxins and their predicted pharmacological activity in the venoms (2 snakes)

List of identified toxins and their predicted pharmacological activity in the venoms 2 snakes

Determination of ADDomers/ADDobodies to neutralize venom-induced lethality using in vivo ED50 assays

Websites, patent fillings, videos etc. (2)

First version of the website
Toxin database

Open Research Data Pilot (1)

Data management plan

Publicaciones

Peer reviewed articles (9)

Virus-like particles displaying conserved toxin epitopes stimulate polyspecific, murine antibody responses capable of snake venom recognition

Autores: Stefanie K. Menzies, Charlotte A. Dawson, Edouard Crittenden, Rebecca J. Edge, Steven R. Hall, Jaffer Alsolaiss, Mark C. Wilkinson, Nicholas R. Casewell, Robert A. Harrison, Stuart Ainsworth
Publicado en: Scientific Reports, Edición 12, 2022, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-022-13376-x

Dual Proteomics Strategies to Dissect and Quantify the Components of Nine Medically Important African Snake Venoms

Autores: Damien Redureau, Fernanda Gobbi Amorim, Thomas Crasset, Imre Berger, Christiane Schaffitzel, Stefanie Kate Menzies, Nicholas R. Casewell, Loïc Quinton
Publicado en: Toxins, Edición 17, 2025, Página(s) 243, ISSN 2072-6651
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins17050243

Virus-like particles displaying conserved toxin epitopes stimulate polyspecific, murine antibody responses capable of snake venom recognition

Autores: Stefanie K. Menzies; Charlotte A. Dawson; Edouard Crittenden; Rebecca J. Edge; Steven R. Hall; Jaffer Alsolaiss; Mark C. Wilkinson; Nicholas R. Casewell; Robert A. Harrison; Stuart Ainsworth
Publicado en: Scientific Reports, 2022, ISSN 0028-0836
Editor: Nature Publishing Group
DOI: 10.1038/s41598-022-13376-x

De novo designed proteins neutralize lethal snake venom toxins

Autores: Susana Vázquez Torres, Melisa Benard Valle, Stephen P. Mackessy, Stefanie K. Menzies, Nicholas R. Casewell, Shirin Ahmadi, Nick J. Burlet, Edin Muratspahić, Isaac Sappington, Max D. Overath, Esperanza Rivera-de-Torre, Jann Ledergerber, Andreas H. Laustsen, Kim Boddum, Asim K. Bera, Alex Kang, Evans Brackenbrough, Iara A. Cardoso, Edouard P. Crittenden, Rebecca J. Edge, Justin Decarreau, Robert J
Publicado en: Nature, Edición 639, 2025, Página(s) 225-231, ISSN 0028-0836
Editor: Nature Publishing Group
DOI: 10.1038/s41586-024-08393-x

Engineering the ADDobody protein scaffold for generation of high-avidity ADDomer super-binders

Autores: Dora Buzas, Huan Sun, Christine Toelzer, Sathish K. N. Yadav, Ufuk Borucu, Gunjan Gautam, Kapil Gupta, Josh Bufton, Julien Capin, Richard B. Sessions, Frederic Garzoni, Imre Berger, Christiane Schaffitzel
Publicado en: Structure, 2023, ISSN 0969-2126
Editor: Cell Press
DOI: 10.1101/2023.09.09.556966

Next-Generation Sequencing for Venomics: Application of Multi-Enzymatic Limited Digestion for Inventorying the Snake Venom Arsenal

Autores: Fernanda Gobbi Amorim, Damien Redureau, Thomas Crasset, Lou Freuville, Dominique Baiwir, Stefanie K. Menzies, Nicholas R. Casewell, Loïc Quinton
Publicado en: Toxins, Edición 15(6), 2023, Página(s) 357, ISSN 2072-6651
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins15060357

Preclinical evaluation of the neutralising efficacy of three antivenoms against the venoms of the recently taxonomically partitioned E. ocellatus and E. romani

Autores: Rebecca J. Edge, Amy E. Marriott, Molly Keen, Tiffany Xie, Edouard P. Crittenden, Charlotte A. Dawson, Mark C. Wilkinson, Wolfgang Wüster, Nicholas R. Casewell, Stuart Ainsworth, Stefanie K. Menzies
Publicado en: PLOS Neglected Tropical Diseases, Edición 19, 2025, Página(s) e0013371, ISSN 1935-2735
Editor: Public Library of Science (PLoS)
DOI: 10.1371/journal.pntd.0013371

In vitro generated antibodies guide thermostable ADDomer nanoparticle design for nasal vaccination and passive immunization against SARS-CoV-2

Autores: Dora Buzas, Adrian H Bunzel, Oskar Staufer, Emily J Milodowski, Grace L Edmunds, Joshua C Bufton, Beatriz V Vidana Mateo, Sathish K N Yadav, Kapil Gupta, Charlotte Fletcher, Maia K Williamson, Alexandra Harrison, Ufuk Borucu, Julien Capin, Ore Francis, Georgia Balchin, Sophie Hall, Mirella V Vega, Fabien Durbesson, Srikanth Lingappa, Renaud Vincentelli, Joe Roe, Linda Wooldridge, Rachel Burt, Ross
Publicado en: Antibody Therapeutics, Edición tbad024, 2023, ISSN 2516-4236
Editor: Oxford Academic
DOI: 10.1093/abt/tbad024

ADDovenom: Thermostable Protein-Based ADDomer Nanoparticles as New Therapeutics for Snakebite Envenoming

Autores: Stefanie K. Menzies, Raquel A. Garcia, Fernanda Gobbi Amorim, Iara Aimê Cardoso,Camille Abada, Thomas Crasset, Rebecca J. Edge, Sophie Hall, Damien Redureau, Richard Stenner, Johara Stringari, António Roldão, Paula M.vAlves, Robert A. Harrison, Renaud Vincentelli, Huan Sun, Imre Berger, Loïc Quinton, Nicholas R. Casewell, Christiane Schaffitzel
Publicado en: Toxins, Edición 15(12), 2023, Página(s) 673, ISSN 2072-6651
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins15120673