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ADDovenom: Novel Snakebite Therapy Platform of Unparalleled Efficacy, Safety and Affordability

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

List of protein / peptide components for 8 African snake venoms and their relative abundance identified by MS (opens in new window)

List of protein peptide components for 8 African snake venoms and their relative abundance identified by MS

White paper 1 (opens in new window)
List of identified toxins and their predicted pharmacological activity in the venoms (8 snakes) (opens in new window)
List of sequences, PTMs, disulphide bridges and isoelectric points of the venom components (2 snakes) (opens in new window)

List of sequences PTMs disulphide bridges and isoelectric points of the venom components 2 snakes

Pharmokinetic characterisation of ADDomer/ADDobodies in mice (opens in new window)
Validated protocols for the production of ADDobodies in high-throughput and milligram amounts (opens in new window)

Validated protocols for the production of ADDobodies in highthroughput and milligram amounts

Determination of venom toxicity (LD50) for each snake species (opens in new window)

Determination of venom toxicity LD50 for each snake species

In process assays for ADDomers (opens in new window)
Demonstration of safety of ADDomer/ADDobodies in mice (opens in new window)
List of sequences, PTMs, disulphide bridges and isoelectric points of the venom components (8 snakes) (opens in new window)
List of protein / peptide components for 2 African snake venoms and their relative abundance identified by MS (opens in new window)

List of protein peptide components for 2 African snake venoms and their relative abundance identified by MS

Validated protocols for the production of toxins/ epitope strings (opens in new window)

Validated protocols for the production of toxins epitope strings

In vitro functional ability of ADDomers/ADDobodies to neutralise toxin-function (opens in new window)
List of identified toxins and their predicted pharmacological activity in the venoms (2 snakes) (opens in new window)

List of identified toxins and their predicted pharmacological activity in the venoms 2 snakes

Determination of ADDomers/ADDobodies to neutralize venom-induced lethality using in vivo ED50 assays (opens in new window)

Publications

Dual Proteomics Strategies to Dissect and Quantify the Components of Nine Medically Important African Snake Venoms (opens in new window)

Author(s): Damien Redureau, Fernanda Gobbi Amorim, Thomas Crasset, Imre Berger, Christiane Schaffitzel, Stefanie Kate Menzies, Nicholas R. Casewell, Loïc Quinton
Published in: Toxins, Issue 17, 2025, Page(s) 243, ISSN 2072-6651
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins17050243

Virus-like particles displaying conserved toxin epitopes stimulate polyspecific, murine antibody responses capable of snake venom recognition (opens in new window)

Author(s): Stefanie K. Menzies; Charlotte A. Dawson; Edouard Crittenden; Rebecca J. Edge; Steven R. Hall; Jaffer Alsolaiss; Mark C. Wilkinson; Nicholas R. Casewell; Robert A. Harrison; Stuart Ainsworth
Published in: Scientific Reports, 2022, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-022-13376-x

De novo designed proteins neutralize lethal snake venom toxins (opens in new window)

Author(s): Susana Vázquez Torres, Melisa Benard Valle, Stephen P. Mackessy, Stefanie K. Menzies, Nicholas R. Casewell, Shirin Ahmadi, Nick J. Burlet, Edin Muratspahić, Isaac Sappington, Max D. Overath, Esperanza Rivera-de-Torre, Jann Ledergerber, Andreas H. Laustsen, Kim Boddum, Asim K. Bera, Alex Kang, Evans Brackenbrough, Iara A. Cardoso, Edouard P. Crittenden, Rebecca J. Edge, Justin Decarreau, Robert J
Published in: Nature, Issue 639, 2025, Page(s) 225-231, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-024-08393-x

Engineering the ADDobody protein scaffold for generation of high-avidity ADDomer super-binders (opens in new window)

Author(s): Dora Buzas, Huan Sun, Christine Toelzer, Sathish K. N. Yadav, Ufuk Borucu, Gunjan Gautam, Kapil Gupta, Josh Bufton, Julien Capin, Richard B. Sessions, Frederic Garzoni, Imre Berger, Christiane Schaffitzel
Published in: Structure, 2023, ISSN 0969-2126
Publisher: Cell Press
DOI: 10.1101/2023.09.09.556966

Next-Generation Sequencing for Venomics: Application of Multi-Enzymatic Limited Digestion for Inventorying the Snake Venom Arsenal (opens in new window)

Author(s): Fernanda Gobbi Amorim, Damien Redureau, Thomas Crasset, Lou Freuville, Dominique Baiwir, Stefanie K. Menzies, Nicholas R. Casewell, Loïc Quinton
Published in: Toxins, Issue 15(6), 2023, Page(s) 357, ISSN 2072-6651
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins15060357

In vitro generated antibodies guide thermostable ADDomer nanoparticle design for nasal vaccination and passive immunization against SARS-CoV-2 (opens in new window)

Author(s): Dora Buzas, Adrian H Bunzel, Oskar Staufer, Emily J Milodowski, Grace L Edmunds, Joshua C Bufton, Beatriz V Vidana Mateo, Sathish K N Yadav, Kapil Gupta, Charlotte Fletcher, Maia K Williamson, Alexandra Harrison, Ufuk Borucu, Julien Capin, Ore Francis, Georgia Balchin, Sophie Hall, Mirella V Vega, Fabien Durbesson, Srikanth Lingappa, Renaud Vincentelli, Joe Roe, Linda Wooldridge, Rachel Burt, Ross
Published in: Antibody Therapeutics, Issue tbad024, 2023, ISSN 2516-4236
Publisher: Oxford Academic
DOI: 10.1093/abt/tbad024

ADDovenom: Thermostable Protein-Based ADDomer Nanoparticles as New Therapeutics for Snakebite Envenoming (opens in new window)

Author(s): Stefanie K. Menzies, Raquel A. Garcia, Fernanda Gobbi Amorim, Iara Aimê Cardoso,Camille Abada, Thomas Crasset, Rebecca J. Edge, Sophie Hall, Damien Redureau, Richard Stenner, Johara Stringari, António Roldão, Paula M.vAlves, Robert A. Harrison, Renaud Vincentelli, Huan Sun, Imre Berger, Loïc Quinton, Nicholas R. Casewell, Christiane Schaffitzel
Published in: Toxins, Issue 15(12), 2023, Page(s) 673, ISSN 2072-6651
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins15120673

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