A multidisciplinary training network for the bioinspired development of glycomimetics tuning the Siglec-Sialoglycan axis

Project Information

GLYTUNES

Grant agreement ID: 956758

DOI

10.3030/956758

Project closed

EC signature date 13 August 2020

Start date 1 March 2021

End date 28 February 2025

Funded under

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Total cost

€ 3 744 223,56

EU contribution

€ 3 744 223,56

Coordinated by

UNIVERSITA DEGLI STUDI DI NAPOLI FEDERICO II
Italy

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Open Research Data Pilot (1)

Data Management Plan

Documents, reports (4)

Recruitment
Min. 40 publications in peer-reviewed journals
SB and SAB established
Dissemination activities to the scientific community:

Websites, patent fillings, videos etc. (1)

Website

Publications

Peer reviewed articles (18)

Insights into Siglec‐7 Binding to Gangliosides: NMR Protein Assignment and the Impact of Ligand Flexibility

Author(s): Cristina Di Carluccio, Luis Padilla‐Cortés, Marta Tiemblo‐Martìn, Giulia Roxana Gheorghita, Rosario Oliva, Linda Cerofolini, Alessandro Antonio Masi, Celeste Abreu, Hsin‐Kai Tseng, Antonio Molinaro, Pompea Del Vecchio, Ondřej Vaněk, Chun‐Cheng Lin, Roberta Marchetti, Marco Fragai, Alba Silipo
Published in: Advanced Science, 2025, ISSN 2198-3844
Publisher: Wiley
DOI: 10.1002/advs.202415782

Quantifying Siglec-sialylated ligand interactions: a versatile ¹⁹F-T₂ CPMG filtered competitive NMR displacement assay

Author(s): Unai Atxabal, Andrea Fernández, Maria Jesús Moure, Klaudia Sobczak, Corwin Nycholat, Verónica Almeida-Marrero, Iker Oyenarte, James C. Paulson, Andrés de la Escosura, Tomás Torres, Niels C. Reichardt, Jesús Jiménez-Barbero, June Ereño-Orbea
Published in: Chemical Science, Issue 15, 2024, Page(s) 10612-10624, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/d4sc01723d

Site-Selective Functionalized PD-1 Mutant for a Modular Immunological Activity against Cancer Cells

Author(s): Silvia Fallarini, Linda Cerofolini, Maria Salobehaj, Domenico Rizzo, Giulia Roxana Gheorghita, Giulia Licciardi, Daniela Eloisa Capialbi, Valerio Zullo, Andrea Sodini, Cristina Nativi*, and Marco Fragai*
Published in: Biomacromolecules, Issue 24, 2023, Page(s) 5428–5437, ISSN 1526-4602
Publisher: American Chemical Society
DOI: 10.1021/acs.biomac.3c00893

Structural insights into Siglec-15 reveal glycosylation dependency for its interaction with T cells through integrin CD11b

Author(s): Maria Pia Lenza, Leire Egia-Mendikute, Asier Antoñana-Vildosola, Cátia O. Soares, Helena Coelho, Francisco Corzana, Alexandre Bosch, Prodhi Manisha, Jon Imanol Quintana, Iker Oyenarte, Luca Unione, María Jesús Moure, Mikel Azkargorta, Unai Atxabal, Klaudia Sobczak, Felix Elortza, James D. Sutherland, Rosa Barrio, Filipa Marcelo, Jesús Jiménez-Barbero, Asis Palazon & June Ereño-Orbea
Published in: Nature Communications, 2023, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-023-39119-8

Combining Solid‐State NMR with Structural and Biophysical Techniques to Design Challenging Protein‐Drug Conjugates

Author(s): Linda Cerofolini; Kristian Vasa; Elisa Bianconi; Maria Salobehaj; Giulia Cappelli; Alice Bonciani; Giulia Licciardi; Anna Pérez‐Ràfols; Luis Padilla‐Cortés; Sabrina Antonacci; Domenico Rizzo; Enrico Ravera; Caterina Viglianisi; Vito Calderone; Giacomo Parigi; Claudio Luchinat; Antonio Macchiarulo; Stefano Menichetti; Marco Fragai
Published in: Angewandte Chemie - International Edition, 2023, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/ange.202303202

UV light-induced spatial loss of sialic acid capping using a photoactivatable sialyltransferase inhibitor

Author(s): Sam J. Moons; Daniël L.A.H. Hornikx; Mikkel K. M. Aasted; Johan F.A. Pijnenborg; Matteo Calzari; Paul B. White; Yoshiki Narimatsu; Henrik Clausen; Hans H. Wandall; Thomas J. Boltje; Christian Büll
Published in: RCS Chemical Biology, Issue 4, 2023, ISSN 2633-0679
Publisher: Royal Society of Chemistry
DOI: 10.1039/d3cb00006k

The sialic acid-Siglec immune checkpoint: an opportunity to enhance immune responses and therapy effectiveness in melanoma

Author(s): Magali Coccimiglio, Fabrizio Chiodo, Yvette van Kooyk
Published in: British Journal of Dermatology, 2024, ISSN 1365-2133
Publisher: Oxford Academic
DOI: 10.1093/bjd/ljad517

Molecular Insights into O-Linked Sialoglycans Recognition by the Siglec-Like SLBR-N (SLBRUB10712) of Streptococcus gordonii

Author(s): Cristina Di Carluccio, Linda Cerofolini, Miguel Moreira, Frédéric Rosu, Luis Padilla-Cortés, Giulia Roxana Gheorghita, Zhuojia Xu, Abhishek Santra, Hai Yu, Shinji Yokoyama, Taylor E. Gray, Chris D. St. Laurent, Yoshiyuki Manabe, Xi Chen, Koichi Fukase, Matthew S. Macauley, Antonio Molinaro, Tiehai Li, Barbara A. Bensing, Roberta Marchetti*, Valérie Gabelica, Marco Fragai, and Alba Silipo*
Published in: ACS Cent. Sci., 2024, Page(s) 447–459, ISSN 2374-7951
Publisher: ACS Publications
DOI: 10.1021/acscentsci.3c01598

Mammalian cell-based production of glycans, glycopeptides and glycomodules

Author(s): Thapakorn Jaroentomeechai, Richard Karlsson, Felix Goerdeler, Fallen Kai Yik Teoh, Magnus Nørregaard Grønset, Dylan de Wit, Yen-Hsi Chen, Sanae Furukawa, Venetia Psomiadou, Ramon Hurtado-Guerrero, Elena Ethel Vidal-Calvo, Ali Salanti, Thomas J. Boltje, Leendert J. van den Bos, Christian Wunder, Ludger Johannes, Katrine T. Schjoldager, Hiren J. Joshi, Rebecca L. Miller, Henrik Clausen, Sergey Y.
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-024-53738-9

Engineering the sialome of mammalian cells with sialic acid mimetics

Author(s): Daniël L A H Hornikx , Eline A Visser , Venetia Psomiadou , Christian Büll , Thomas J Boltje
Published in: STAR Protocols, 2023, ISSN 2666-1667
Publisher: Elsevier Inc
DOI: 10.1016/j.xpro.2023.102330

Sialic acids in infection and their potential use in detection and protection against pathogens

Author(s): Simone Dedola, Sanaz Ahmadipour,a Peterson de Andrade, Alexander N. Baker, Andrew N. Boshra, Simona Chessa, Matthew I. Gibson, Pedro J. Hernando, Irina M. Ivanova,b Jessica E. Lloyd,María J. Marín, O Alexandra J. Munro-Clark, Giulia Pergolizzi, Sarah-Jane Richards, Iakovia Ttofi, Ben A. Wagstaffa and Robert A. Field
Published in: RSC Chemical Biology, 2023, ISSN 2633-0679
Publisher: Royal Society of Chemistry
DOI: 10.1039/d3cb00155e

The lipooligosaccharide of the gut symbiont Akkermansia muciniphila exhibits a remarkable structure and TLR signaling capacity

Author(s): Pilar Garcia-Vello, Hanne L. P. Tytgat, Janneke Elzinga, Matthias Van Hul, Hubert Plovier, Marta Tiemblo-Martin, Patrice D. Cani, Simone Nicolardi, Marco Fragai, Cristina De Castro, Flaviana Di Lorenzo, Alba Silipo, Antonio Molinaro, Willem M. de Vos
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-024-52683-x

Structure and properties of the exopolysaccharide isolated from Flavobacterium sp. Root935

Author(s): Marta Tiemblo-Martín, Valeria Pistorio, Pia Saake, Lisa Mahdi, María Asunción Campanero-Rhodes, Rocco Di Girolamo, Cristina Di Carluccio, Roberta Marchetti, Antonio Molinaro, Dolores Solís, Alga Zuccaro, Alba Silipo
Published in: Carbohydrate Polymers, Issue 343, 2024, Page(s) 122433, ISSN 0144-8617
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.carbpol.2024.122433

Solid-state NMR – a complementary technique for protein framework characterization

Author(s): Cerofolini, Linda; Ramberg, Kiefer O.; Padilla, Luis C.; Antonik, Paweł; Ravera, Enrico; Luchinat, Claudio; Fragai, Marco; Crowley, Peter
Published in: Chemical Communications, Issue 59, 2023, Page(s) 776-779, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/d2cc05725e

Deciphering the Chemical Language of the Immunomodulatory Properties of <i>Veillonella parvula</i> Lipopolysaccharide

Author(s): Molly Dorothy Pither, Emanuela Andretta, Giuseppe Rocca, Fabio Balzarini, Alejandra Matamoros‐Recio, Roberta Colicchio, Paola Salvatore, Yvette van Kooyk, Alba Silipo, Francesca Granucci, Sonsoles Martin‐Santamaria, Fabrizio Chiodo, Antonio Molinaro, Flaviana Di Lorenzo
Published in: Angewandte Chemie International Edition, Issue 63, 2024, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202401541

Parametric Analysis of Donor Activation for Glycosylation Reactions

Author(s): Mei‐Huei Lin, Yan‐Ting Kuo, José Danglad‐Flores, Eric T. Sletten, Peter H. Seeberger
Published in: Chemistry – A European Journal, Issue 30, 2024, ISSN 0947-6539
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/chem.202400479

A photoaffinity glycan-labeling approach to investigate immunoglobulin glycan-binding partners

Author(s): Miles D Holborough-Kerkvliet; Greta Mucignato; Sam J Moons; Venetia Psomiadou; Rohit S R Konada; Nichole J Pedowitz; Matthew R Pratt; Theresa Kissel; Carolien A M Koeleman; Rayman T N Tjokrodirijo; Petrus A van Veelen; Thomas Huizinga; Karin A J van Schie; Manfred Wuhrer; Jennifer J Kohler; Kimberly M Bonger; Thomas J Boltje; Reinaldus E M Toes
Published in: Glycobiology, Issue 9, 2023, Page(s) 732–744, ISSN 1460-2423
Publisher: Oxford University Press
DOI: 10.1093/glycob/cwad055

The human gut Bacteroides eggerthii expresses a new galactofuranose-containing lipooligosaccharide with weak immunostimulatory properties

Author(s): Marta Tiemblo Martín, Magali Coccimiglio, Emanuela Andretta, Luca De Simone Carone, Andrew Bell, Tania Gerpe-Amor, Cristina Di Carluccio, Antonio Molinaro, Yvette van Kooyk, Nathalie Juge, Fabrizio Chiodo, Flaviana Di Lorenzo, Alba Silipo
Published in: Carbohydrate Polymers, Issue 348, 2024, Page(s) 122833, ISSN 0144-8617
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.carbpol.2024.122833