Objective
Objectives and content of the project During seed germination in plants, the nitrogen supplied to the growing embryo is mostly in form of peptides transported from the endosperm. These small peptides are a consequence of proteolytic activities present in the seed endosperm. The objective of this project is to study this peptide transport process starting through the cloning and characterization of wheat peptide transporter by functional complementation in well-characterized yeast mutants. This project will let understand how the wheat embryo obtain the nitrogenous compounds, required for growth, from protein storage reserves in the endosperm of the seed. We plan to carry out: 1. Isolation of wheat cDNA clones by functional complementation of yeast peptide transport mutants. 2. Sequence analysis of the cDNA selected. 3. Characterization of wheat peptide transporter in yeast transformants. 4. Site-specific mutagenesis and analysis in yeast transformants. 5. Expression pattern of wheat transporter in wheat. Training content (objective, benefit and expected impact) Molecular cloning of higher plant genes by functional complementation in yeast mutants is a powerful and original tool which allows us to circumvent the problems associated with traditional techniques. We propose, using this approach, the isolation and characterization of wheat peptide transporter genes. The results obtained in this work can yield substancial information regarding the role that they can play in normal plant growth and development which can open new perspectives in the field of biotechnological applications. On the other hand, this is an ambitious project that will allow me to apply, in Dr. Cejudo's laboratory, all the scientific techniques and skill acquired during my postdoctoral stay in France working on Saccharomyces cerevisiae. Links with industry/ industrial relevance This is an industrially relevant proposal because of the important applications of this crop plant. The host institution, particularly the Dr. Cejudo's lab, has ongoing collaboration in the subject of the project with the french company Rhone- Poulenc. This collaboration is financed by the Spain Programme for Collaboration betwen Industry and Public Research Centers .
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences biochemistry biomolecules proteins
- agricultural sciences agriculture, forestry, and fisheries agriculture
- medical and health sciences clinical medicine embryology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
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Coordinator
54006 Thessaloniki
Greece
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.