Objective
A major challenge in post genome biology is the functional assignment of gene products. An important goal is the identification and characterization of DNA binding proteins – especially transcription factors – and of their specific target sequences. Recent strategies for determining protein-DNA binding specificities combine experimental high throughput data with bioinformatics algorithms providing encouraging results. Structure based approaches are particularly promising, as they can predict previously undetected binding sites and open the road to the rational design of novel regulatory molecules. This project aims at expanding our current knowledge of protein-DNA binding modes. We will use structural bioinformatics methods for predicting the structure and specificity of DNA binding proteins, focusing in particular on natively unfolded protein regions (flexible segments that do not assume a fixed conformation in the native state, but become ordered upon binding) which are frequently observed in DNA binding proteins. We will first develop a general method for predicting the atomic coordinates of the DNA bound conformation of these proteins combining fragment based methods for protein structure prediction and novel DNA-protein specific energy potentials. Predicted complexes will be subsequently used to predict DNA binding sites in genomes and the accuracy assessed on the basis of available experimental reference data. The results of this project will improve our understanding of the molecular details of protein-DNA interactions, assist the annotation of genomes, and prioritize experiments aimed at verifying the predicted specificities. On a more general level, this research will further advance knowledge in the field of macromolecular interactions, a central problem in systems biology.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-IEF-2008
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
WC1E 6BT LONDON
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.