Dopaminergic neurons and the reward system in fish: a functional neuroanatomical multidisciplinary study

Información del proyecto

FISHDOPA

Identificador del acuerdo de subvención: 660477

DOI

10.3030/660477

Proyecto cerrado

Fecha de la firma de la CE 7 Abril 2015

Fecha de inicio 1 Marzo 2016

Fecha de finalización 28 Febrero 2018

Financiado con arreglo a

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Coste total

€ 185 857,20

Aportación de la UE

€ 185 857,20

Coordinado por

GOETEBORGS UNIVERSITET
Sweden

Objetivo

The current proposal aims at elucidating the role of the dopaminergic (DA) system in regulation and control of reward systems. The study of DA regulation is of great importance since it has been known to regulate reward, as well as other vital neurological functions and several neuropsychological illnesses, but the underlying molecular mechanisms are still not fully understood. With the emerging technologies of in vivo imaging, optogenetics and transgenic techniques, it is today possible to better study the molecular mechanisms underlying reward behaviour. To this end, the zebrafish (Danio rerio) is an excellent model system where these tools are amenable for complex network analyses. It is believed that, as in mammals, DA neurons provide a central role in signal and response to salient stimuli. Therefore in this project we plan to 1. Establish which forebrain DA neuronal populations, brain targets and signalling molecules are active during reward conditions and
2. Establish a causal relationship between these areas and the control of reward-motivated behaviour in fish. We will conduct this research by pinpointing DA neuron populations active during reward situations and their target areas by in vivo calcium imaging analysis and corroborating established focus brain networks by quantifying c-Fos and brain-derived neurotrophic factor (BDNF) expression by means of in situ hybridization (WP1). Thereafter, we will elucidate target molecules that are important during reward processing in established networks, by laser microdissection and a targeted and non-targeted approach, i.e. qPCR and RNA sequencing, respectively (WP2). Finally we will demonstrate causality between focus brain networks and reward oriented behaviour by optical and genetical regulation, i.e. optogenetics, of neural population activity (WP3). Extrapolating information obtained from these results could help predict neurobiological principles that could be the basis for therapeutic interventions.

Ámbito científico (EuroSciVoc)

• ciencias naturales › ciencias biológicas › neurobiología
• ciencias naturales › ciencias químicas › química inorgánica › metales alcalinotérreos
• ciencias naturales › matemáticas › matemáticas puras › análisis matemático › análisis funcional
• ciencias naturales › ciencias biológicas › zoología › mamalogía
• ciencias naturales › ciencias físicas › óptica › física del láser

Programa(s)

• H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions PROGRAMA PRINCIPAL
  
• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
  

Tema(s)

• MSCA-IF-2014-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF)
  

Régimen de financiación

MSCA-IF-EF-ST - Standard EF

Convocatoria de propuestas

H2020-MSCA-IF-2014

Coordinador

GOETEBORGS UNIVERSITET

• Aportación neta de la UEn: € 185 857,20
• Dirección: VASAPARKEN ; 405 30 Goeteborg ; Suecia
• Región: Södra Sverige › Västsverige › Västra Götalands län
• Tipo de actividad: Higher or Secondary Education Establishments
• Coste total: € 185 857,20