ENable LIGHT- and synthetic biology-driven volumetric bioprinting of functional human tissues

Información del proyecto

ENLIGHT

Identificador del acuerdo de subvención: 964497

DOI

10.3030/964497

Proyecto cerrado

Fecha de la firma de la CE 22 Enero 2021

Fecha de inicio 1 Mayo 2021

Fecha de finalización 30 Abril 2025

Financiado con arreglo a

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coste total

€ 3 594 925,00

Aportación de la UE

€ 3 594 925,00

Coordinado por

UNIVERSITAIR MEDISCH CENTRUM UTRECHT
Netherlands

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Websites, patent fillings, videos etc. (1)

ENLIGHT logo and website

The ENLIGHT logo and first version of the project website will be delivered at M2Task involved T62

Documents, reports (2)

Infographs describing the project and its impact

After the first year infographs of the project and its impact will be made public through the website The infographs will be updated on the website throughout the projectTask involved T62

Management handbook

A management handbook will be written including templates meeting schedules and a detailed planning of activities as well as decisionmaking processesTasks involved T71 and T72

Publicaciones

Peer reviewed articles (37)

Smartphone-Flashlight-Mediated Remote Control of Rapid Insulin Secretion Restores Glucose Homeostasis in Experimental Type-1 Diabetes

Autores: Mansouri, Maysam; Xue, Shuai; Hussherr, Marie-Didiée; Strittmatter, Tobias; Camenisch, Gieri; Fussenegger, Martin
Publicado en: Small, Edición 4, 2021, ISSN 1613-6829
Editor: Wiley
DOI: 10.1002/smll.202101939

3D<i>in vitro</i>modeling of the exocrine pancreatic unit using tomographic volumetric bioprinting

Autores: Viola Sgarminato, Jorge Madrid-Wolff, Antoine Boniface, Gianluca Ciardelli, Chiara Tonda-Turo, Christophe Moser
Publicado en: Biofabrication, 2024, ISSN 1758-5090
Editor: IOP Publishing Ltd
DOI: 10.1101/2023.01.23.525142

Light from Afield: Fast, High-Resolution, and Layer-Free Deep Vat 3D Printing

Autores: Chansoria, Parth; id_orcid0000-0002-6107-6848; Rizzo, Riccardo; id_orcid0000-0001-8297-6776; Rütsche, Dominic; Liu, Hao; Delrot, Paul; Zenobi-Wong, Marcy; id_orcid0000-0002-8522-9909
Publicado en: Chemical Reviews, 124 (14), Edición 1, 2024, ISSN 0009-2665
Editor: American Chemical Society

Bioprinting of Human Liver‐Derived Epithelial Organoids for Toxicity Studies

Autores: Manon C. Bouwmeester, Paulina N. Bernal, Loes A. Oosterhoff, Monique E. van Wolferen, Vivian Lehmann, Monique Vermaas, Maj‐Britt Buchholz, Quentin C. Peiffer, Jos Malda, Luc J. W. van der Laan, Nynke I. Kramer, Kerstin Schneeberger, Riccardo Levato, Bart Spee
Publicado en: Macromolecular Bioscience, Edición 21, 2023, ISSN 1616-5187
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/mabi.202100327

Liver Organoids: Updates on Disease Modeling and Biomedical Applications

Autores: Carmen Caiazza, Silvia Parisi, Massimiliano Caiazzo
Publicado en: Biology, Edición 10, 2024, Página(s) 835, ISSN 2079-7737
Editor: MDPI AG
DOI: 10.3390/biology10090835

Liver Organoids: Updates on Disease Modeling and Biomedical Applications.

Autores: Caiazza, Carmen; Parisi, Silvia; Caiazzo, Massimiliano; Afd Pharmaceutics; Pharmaceutics
Publicado en: Biology, Edición 1, 2021, ISSN 2079-7737
Editor: MDPI
DOI: 10.3390/biology10090835

Volumetric Bioprinting of Organoids and Optically Tuned Hydrogels to Build Liver-Like Metabolic Biofactories

Autores: Paulina; Manon; Jorge; Marc; Sammy; Nuria; Gabriel; Roos-Anne; Monique; Luc; Paul; Damien; Jos; Christophe; Bart; Riccardo
Publicado en: Advanced Materials, Edición 1, 2022, ISSN 1521-4095
Editor: Wiley-VCH
DOI: 10.5281/zenodo.6380100

Receptor-mediated transcytosis of nanobodies targeting the heparin-binding EGF-like growth factor in human blood-brain barrier models

Autores: Boning Qiu, Sara Pompe, Katerina T. Xenaki, Alessia Di Maggio, Clara Belinchón Moreno, Paul M.P. van Bergen en Henegouwen, Enrico Mastrobattista, Sabrina Oliveira, Massimiliano Caiazzo
Publicado en: Journal of Controlled Release, Edición 383, 2025, Página(s) 113852, ISSN 0168-3659
Editor: Elsevier BV
DOI: 10.1016/j.jconrel.2025.113852

Nitroglycerin-responsive gene switch for the on-demand production of therapeutic proteins

Autores: Mohamed Mahameed, Shuai Xue, Benjamin Danuser, Ghislaine Charpin-El Hamri, Mingqi Xie, Martin Fussenegger
Publicado en: Nature Biomedical Engineering, 2025, ISSN 2157-846X
Editor: Springer Science and Business Media LLC
DOI: 10.1038/s41551-025-01350-7

Glucose‐Operated Widget (GLOW) for Closed‐Loop Optogenetic Glycemic Control

Autores: Debasis Maity, Preetam Guha Ray, Martin Fussenegger
Publicado en: Advanced Materials, Edición 36, 2024, ISSN 0935-9648
Editor: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202408537

Light-based vat-polymerization bioprinting

Autores: Riccardo Levato, Oksana Dudaryeva, Carlos Ezio Garciamendez-Mijares, Bruce E. Kirkpatrick, Riccardo Rizzo, Jacob Schimelman, Kristi S. Anseth, Shaochen Chen, Marcy Zenobi-Wong, Yu Shrike Zhang
Publicado en: Nature Reviews Methods Primers, Edición 3, 2024, ISSN 2662-8449
Editor: Nature
DOI: 10.1038/s43586-023-00231-0

Light-based vat-polymerization bioprinting

Autores: Riccardo Levato; Oksana Dudaryeva; Carlos Ezio Garciamendez-Mijares; Bruce E. Kirkpatrick; Riccardo Rizzo; Jacob Schimelman; Kristi S. Anseth; Shaochen Chen; Marcy Zenobi-Wong; Yu Shrike Zhang
Publicado en: Nature Reviews Methods Primers, 3, Edición 1, 2023, ISSN 2662-8449
Editor: Nature
DOI: 10.1038/s43586-023-00231-0

Shaping Synthetic Multicellular and Complex Multimaterial Tissues via Embedded Extrusion-Volumetric Printing of Microgels

Autores: Davide Ribezzi, Marième Gueye, Sammy Florczak, Franziska Dusi, Dieuwke de Vos, Francesca Manente, Andreas Hierholzer, Martin Fussenegger, Massimiliano Caiazzo, Torsten Blunk, Jos Malda, Riccardo Levato
Publicado en: Advanced Materials, 2023, ISSN 0935-9648
Editor: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202301673

Engineering electrogenetic interfaces for mammalian cell control

Autores: Maysam Mansouri, Martin Fussenegger
Publicado en: Cell Chemical Biology, Edición 32, 2025, Página(s) 521-528, ISSN 2451-9456
Editor: Elsevier BV
DOI: 10.1016/j.chembiol.2025.01.003

A review of materials used in tomographic volumetric additive manufacturing

Autores: Jorge Madrid-Wolff, Joseph Toombs, Riccardo Rizzo, Paulina Nuñez Bernal, Dominique Porcincula, Rebecca Walton, Bin Wang, Frederik Kotz-Helmer, Yi Yang, David Kaplan, Yu Shrike Zhang, Marcy Zenobi-Wong, Robert R. McLeod, Bastian Rapp, Johanna Schwartz, Maxim Shusteff, Hayden Talyor, Riccardo Levato, Christophe Moser
Publicado en: MRS Communications, Edición 13, 2023, Página(s) 764-785, ISSN 2159-6867
Editor: Springer Science and Business Media LLC
DOI: 10.1557/s43579-023-00447-x

Smart-watch-programmed green-light-operated percutaneous control of therapeutic transgenes

Autores: Maysam Mansouri, Marie-Didiée Hussherr, Tobias Strittmatter, Peter Buchmann, Shuai Xue, Gieri Camenisch, Martin Fussenegger
Publicado en: Nature Communications, Edición 12/1, 2021, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-021-23572-4

Enhancing cell‐based therapies with synthetic gene circuits responsive to molecular stimuli

Autores: Silvia Galvan, Ana P. Teixeira, Martin Fussenegger
Publicado en: Biotechnology and Bioengineering, Edición 121, 2025, Página(s) 2987-3000, ISSN 0006-3592
Editor: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.28770

Bioprinting and synthetic biology approaches to engineer functional endocrine pancreatic constructs

Autores: Davide Ribezzi, Pere Català, Cataldo Pignatelli, Antonio Citro, Riccardo Levato
Publicado en: Trends in Biotechnology, 2025, ISSN 0167-7799
Editor: Elsevier BV
DOI: 10.1016/j.tibtech.2025.03.005

Delivery Systems in Neuronal Direct Cell Reprogramming

Autores: Giulia Redi, Filomena Del Piano, Sara Cappellini, Martina Paladino, Anne den Breejen, Marcel H.A.M. Fens, Massimiliano Caiazzo
Publicado en: Cellular Reprogramming, 2025, ISSN 2152-4971
Editor: Mary Ann Liebert Inc.
DOI: 10.1089/cell.2025.0008

Brain Organoids: Filling the Need for a Human Model of Neurological Disorder

Autores: Philip Jalink, Massimiliano Caiazzo
Publicado en: Biology, Edición 10, 2025, Página(s) 740, ISSN 2079-7737
Editor: MDPI
DOI: 10.3390/biology10080740

A review of materials used in tomographic volumetric additive manufacturing

Autores: Jorge Madrid-Wolff; Joseph Toombs; Riccardo Rizzo; Paulina Nuñez Bernal; Dominique Porcincula; Rebecca Walton; Bin Wang; Frederik Kotz-Helmer; Yi Yang; David Kaplan; Yu Shrike Zhang; Marcy Zenobi-Wong; Robert R. McLeod; Bastian Rapp; Johanna Schwartz; Maxim Shusteff; Hayden Talyor; Riccardo Levato; Christophe Moser
Publicado en: MRS Communications, Edición 3, 2023, ISSN 2159-6867
Editor: Springer Link
DOI: 10.1557/s43579-023-00447-x

MicroRNA Roles in Cell Reprogramming Mechanisms

Autores: Pascale, Emilia; Caiazza, Carmen; Paladino, Martina; Parisi, Silvia; Passaro, Fabiana; Caiazzo, Massimiliano
Publicado en: Cells, Edición 3, 2022, ISSN 2073-4409
Editor: MDPI
DOI: 10.3390/cells11060940

Breaking the resolution limits of 3D bioprinting: future opportunities and present challenges

Autores: Tommaso Zandrini, Sammy Florczak, Riccardo Levato, and Aleksandr Ovsianikov
Publicado en: Trends in Biotechnology, 2022, ISSN 0167-7799
Editor: Elsevier BV
DOI: 10.1016/j.tibtech.2022.10.009

Induced pluripotent stem cell-derived and directly reprogrammed neurons to study neurodegenerative diseases: The impact of aging signatures

Autores: Simona Aversano, Carmen Caiazza, Massimiliano Caiazzo
Publicado en: Frontiers in Aging Neuroscience, 2022, ISSN 1663-4365
Editor: Frontiers Research Foundation
DOI: 10.3389/fnagi.2022.1069482

Levodopa-loaded nanoparticles for the treatment of Parkinson's disease

Autores: Emile F. van Vliet, Maarten J. Knol, Raymond M. Schiffelers, Massimiliano Caiazzo, Marcel H.A.M. Fens
Publicado en: Journal of Controlled Release, Edición 360, 2023, Página(s) 212-224, ISSN 0168-3659
Editor: Elsevier BV
DOI: 10.1016/j.jconrel.2023.06.026

Volumetric Bioprinting of Organoids and Optically Tuned Hydrogels to Build Liver-Like Metabolic Biofactor

Autores: Paulina Nuñez Bernal,Manon Bouwmeester,Jorge Madrid-Wolff,Marc Falandt,Sammy Florczak,Nuria Ginés Rodriguez,Yang Li,Gabriel Größbacher,Roos-Anne Samsom,Monique van Wolferen,Luc J. W. van der Laan,Paul Delrot,Damien Loterie,Jos Malda,Christophe Moser,Bart Spee,Riccardo Levato
Publicado en: Advanced Materials, 2022, ISSN 0935-9648
Editor: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202110054

Programming Delayed Dissolution Into Sacrificial Bioinks For Dynamic Temporal Control of Architecture within 3D-Bioprinted Constructs

Autores: Bram G. Soliman, Alessia Longoni, Mian Wang, Wanlu Li, Paulina N. Bernal, Alessandro Cianciosi, Gabriella C.J. Lindberg, Jos Malda, Juergen Groll, Tomasz Jungst, Riccardo Levato, Jelena Rnjak-Kovacina, Tim B. F. Woodfield, Yu Shrike Zhang, Khoon S. Lim
Publicado en: Advanced Functional Materials, 2023, ISSN 1616-301X
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/adfm.202210521

Design of programmable post-translational switch control platform for on-demand protein secretion in mammalian cells

Autores: Maysam Mansouri, Preetam Guha Ray, Nik Franko, Shuai Xue, Martin Fussenegger
Publicado en: Nucleic Acids Research, 2023, ISSN 1362-4962
Editor: Oxford University Press
DOI: 10.1093/nar/gkac916

Controlling Light in Scattering Materials for Volumetric Additive Manufacturing

Autores: Madrid-Wolff, Jorge; Boniface, Antoine; Loterie, Damien; Delrot, Paul; Moser, Christophe
Publicado en: Advanced Science, Edición 1, 2022, ISSN 2198-3844
Editor: Wiley
DOI: 10.1002/advs.202105144

Therapeutic cell engineering: designing programmable synthetic genetic circuits in mammalian cells

Autores: Maysam Mansouri, Martin Fussenegger
Publicado en: Protein &amp; Cell, Edición 13, 2023, Página(s) 476-489, ISSN 1674-8018
Editor: Oxford University Press (OUP)
DOI: 10.1007/s13238-021-00876-1

Small‐Molecule Regulators for Gene Switches to Program Mammalian Cell Behaviour

Autores: Maysam Mansouri, Martin Fussenegger
Publicado en: ChemBioChem, Edición 25, 2025, ISSN 1439-4227
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202300717

Light from Afield: Fast, High-Resolution, and Layer-Free Deep Vat 3D Printing

Autores: Parth Chansoria, Riccardo Rizzo, Dominic Rütsche, Hao Liu, Paul Delrot, Marcy Zenobi-Wong
Publicado en: Chemical Reviews, Edición 124, 2024, Página(s) 8787-8822, ISSN 0009-2665
Editor: American Chemical Society
DOI: 10.1021/acs.chemrev.4c00134

Engineering receptors in the secretory pathway for orthogonal signalling control

Autores: Mohamed Mahameed, Pengli Wang, Shuai Xue & Martin Fussenegger
Publicado en: Nature Communications, 2022, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-022-35161-0

Volumetric Printing Across Melt Electrowritten Scaffolds Fabricates Multi-Material Living Constructs with Tunable Architecture and Mechanics

Autores: Gabriel Größbacher, Michael Bartolf-Kopp, Csaba Gergely, Paulina Núñez Bernal, Sammy Florczak, Mylène de Ruijter, Núria Ginés Rodriguez, Jürgen Groll, Jos Malda, Tomasz Jungst, Riccardo Levato
Publicado en: Advanced Materials, 2023, ISSN 0935-9648
Editor: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202300756

Inverse Rendering for Tomographic Volumetric Additive Manufacturing

Autores: Baptiste Nicolet, Felix Wechsler, Jorge Madrid-Wolff, Christophe Moser, Wenzel Jakob
Publicado en: ACM Transactions on Graphics, Edición 43, 2024, Página(s) 1-17, ISSN 0730-0301
Editor: Association for Computing Machinary, Inc.
DOI: 10.1145/3687924

The road ahead in materials and technologies for volumetric 3D printing

Autores: Paulina Nunez Bernal, Sammy Florczak, Sebastian Inacker, Xiao Kuang, Jorge Madrid-Wolff, Martin Regehly, Stefan Hecht, Yu Shrike Zhang, Christophe Moser, Riccardo Levato
Publicado en: Nature Reviews Materials, 2025, ISSN 2058-8437
Editor: Springer Science and Business Media LLC
DOI: 10.1038/s41578-025-00785-3

Biofabricating the vascular tree in engineered bone tissue

Autores: Leanne de Silva, Paulina N. Bernal, AJW Rosenberg, Jos Malda, Riccardo Levato, Debby Gawlitta
Publicado en: Acta Biomaterialia, 2023, Página(s) 250-268, ISSN 1742-7061
Editor: Elsevier BV
DOI: 10.1016/j.actbio.2022.08.051

Other (1)

Exploring RRI in Bioprinted Organoids: Reflections from the ENLIGHT project

Autores: Simone, Angela; Pellizzone, Anna
Publicado en: Edición 1, 2025
Editor: Zenodo
DOI: 10.5281/zenodo.15372410

Book chapters (1)

Posttranslational Remote Control Mediated by Physical Inducers for Rapid Protein Release in Engineered Mammalian Cells

Autores: Maysam Mansouri, Martin Fussenegger
Publicado en: Methods in Molecular Biology, Mammalian Synthetic Systems, 2024, Página(s) 233-241, ISBN 978-1-0716-3718-0
Editor: Springer US
DOI: 10.1007/978-1-0716-3718-0_15

Non-peer reviewed articles (1)

Multi‐material Volumetric Bioprinting and Plug‐and‐play Suspension Bath Biofabrication via Bioresin Molecular Weight Tuning and via Multiwavelength Alignment Optics

Autores: Davide Ribezzi, Jan‐Philip Zegwaart, Thomas Van Gansbeke, Aitor Tejo‐Otero, Sammy Florczak, Joska Aerts, Paul Delrot, Andreas Hierholzer, Martin Fussenegger, Jos Malda, Jos Olijve, Riccardo Levato
Publicado en: Advanced Materials, Edición 37, 2025, ISSN 0935-9648
Editor: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202409355