ENable LIGHT- and synthetic biology-driven volumetric bioprinting of functional human tissues

Project Information

ENLIGHT

Grant agreement ID: 964497

DOI

10.3030/964497

Project closed

EC signature date 22 January 2021

Start date 1 May 2021

End date 30 April 2025

Funded under

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Total cost

€ 3 594 925,00

EU contribution

€ 3 594 925,00

Coordinated by

UNIVERSITAIR MEDISCH CENTRUM UTRECHT
Netherlands

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Websites, patent fillings, videos etc. (1)

ENLIGHT logo and website

The ENLIGHT logo and first version of the project website will be delivered at M2Task involved T62

Documents, reports (2)

Infographs describing the project and its impact

After the first year infographs of the project and its impact will be made public through the website The infographs will be updated on the website throughout the projectTask involved T62

Management handbook

A management handbook will be written including templates meeting schedules and a detailed planning of activities as well as decisionmaking processesTasks involved T71 and T72

Publications

Peer reviewed articles (37)

Smartphone-Flashlight-Mediated Remote Control of Rapid Insulin Secretion Restores Glucose Homeostasis in Experimental Type-1 Diabetes

Author(s): Mansouri, Maysam; Xue, Shuai; Hussherr, Marie-Didiée; Strittmatter, Tobias; Camenisch, Gieri; Fussenegger, Martin
Published in: Small, Issue 4, 2021, ISSN 1613-6829
Publisher: Wiley
DOI: 10.1002/smll.202101939

3D<i>in vitro</i>modeling of the exocrine pancreatic unit using tomographic volumetric bioprinting

Author(s): Viola Sgarminato, Jorge Madrid-Wolff, Antoine Boniface, Gianluca Ciardelli, Chiara Tonda-Turo, Christophe Moser
Published in: Biofabrication, 2024, ISSN 1758-5090
Publisher: IOP Publishing Ltd
DOI: 10.1101/2023.01.23.525142

Light from Afield: Fast, High-Resolution, and Layer-Free Deep Vat 3D Printing

Author(s): Chansoria, Parth; id_orcid0000-0002-6107-6848; Rizzo, Riccardo; id_orcid0000-0001-8297-6776; Rütsche, Dominic; Liu, Hao; Delrot, Paul; Zenobi-Wong, Marcy; id_orcid0000-0002-8522-9909
Published in: Chemical Reviews, 124 (14), Issue 1, 2024, ISSN 0009-2665
Publisher: American Chemical Society

Bioprinting of Human Liver‐Derived Epithelial Organoids for Toxicity Studies

Author(s): Manon C. Bouwmeester, Paulina N. Bernal, Loes A. Oosterhoff, Monique E. van Wolferen, Vivian Lehmann, Monique Vermaas, Maj‐Britt Buchholz, Quentin C. Peiffer, Jos Malda, Luc J. W. van der Laan, Nynke I. Kramer, Kerstin Schneeberger, Riccardo Levato, Bart Spee
Published in: Macromolecular Bioscience, Issue 21, 2023, ISSN 1616-5187
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/mabi.202100327

Liver Organoids: Updates on Disease Modeling and Biomedical Applications

Author(s): Carmen Caiazza, Silvia Parisi, Massimiliano Caiazzo
Published in: Biology, Issue 10, 2024, Page(s) 835, ISSN 2079-7737
Publisher: MDPI AG
DOI: 10.3390/biology10090835

Liver Organoids: Updates on Disease Modeling and Biomedical Applications.

Author(s): Caiazza, Carmen; Parisi, Silvia; Caiazzo, Massimiliano; Afd Pharmaceutics; Pharmaceutics
Published in: Biology, Issue 1, 2021, ISSN 2079-7737
Publisher: MDPI
DOI: 10.3390/biology10090835

Volumetric Bioprinting of Organoids and Optically Tuned Hydrogels to Build Liver-Like Metabolic Biofactories

Author(s): Paulina; Manon; Jorge; Marc; Sammy; Nuria; Gabriel; Roos-Anne; Monique; Luc; Paul; Damien; Jos; Christophe; Bart; Riccardo
Published in: Advanced Materials, Issue 1, 2022, ISSN 1521-4095
Publisher: Wiley-VCH
DOI: 10.5281/zenodo.6380100

Receptor-mediated transcytosis of nanobodies targeting the heparin-binding EGF-like growth factor in human blood-brain barrier models

Author(s): Boning Qiu, Sara Pompe, Katerina T. Xenaki, Alessia Di Maggio, Clara Belinchón Moreno, Paul M.P. van Bergen en Henegouwen, Enrico Mastrobattista, Sabrina Oliveira, Massimiliano Caiazzo
Published in: Journal of Controlled Release, Issue 383, 2025, Page(s) 113852, ISSN 0168-3659
Publisher: Elsevier BV
DOI: 10.1016/j.jconrel.2025.113852

Nitroglycerin-responsive gene switch for the on-demand production of therapeutic proteins

Author(s): Mohamed Mahameed, Shuai Xue, Benjamin Danuser, Ghislaine Charpin-El Hamri, Mingqi Xie, Martin Fussenegger
Published in: Nature Biomedical Engineering, 2025, ISSN 2157-846X
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/s41551-025-01350-7

Glucose‐Operated Widget (GLOW) for Closed‐Loop Optogenetic Glycemic Control

Author(s): Debasis Maity, Preetam Guha Ray, Martin Fussenegger
Published in: Advanced Materials, Issue 36, 2024, ISSN 0935-9648
Publisher: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202408537

Light-based vat-polymerization bioprinting

Author(s): Riccardo Levato, Oksana Dudaryeva, Carlos Ezio Garciamendez-Mijares, Bruce E. Kirkpatrick, Riccardo Rizzo, Jacob Schimelman, Kristi S. Anseth, Shaochen Chen, Marcy Zenobi-Wong, Yu Shrike Zhang
Published in: Nature Reviews Methods Primers, Issue 3, 2024, ISSN 2662-8449
Publisher: Nature
DOI: 10.1038/s43586-023-00231-0

Light-based vat-polymerization bioprinting

Author(s): Riccardo Levato; Oksana Dudaryeva; Carlos Ezio Garciamendez-Mijares; Bruce E. Kirkpatrick; Riccardo Rizzo; Jacob Schimelman; Kristi S. Anseth; Shaochen Chen; Marcy Zenobi-Wong; Yu Shrike Zhang
Published in: Nature Reviews Methods Primers, 3, Issue 1, 2023, ISSN 2662-8449
Publisher: Nature
DOI: 10.1038/s43586-023-00231-0

Shaping Synthetic Multicellular and Complex Multimaterial Tissues via Embedded Extrusion-Volumetric Printing of Microgels

Author(s): Davide Ribezzi, Marième Gueye, Sammy Florczak, Franziska Dusi, Dieuwke de Vos, Francesca Manente, Andreas Hierholzer, Martin Fussenegger, Massimiliano Caiazzo, Torsten Blunk, Jos Malda, Riccardo Levato
Published in: Advanced Materials, 2023, ISSN 0935-9648
Publisher: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202301673

Engineering electrogenetic interfaces for mammalian cell control

Author(s): Maysam Mansouri, Martin Fussenegger
Published in: Cell Chemical Biology, Issue 32, 2025, Page(s) 521-528, ISSN 2451-9456
Publisher: Elsevier BV
DOI: 10.1016/j.chembiol.2025.01.003

A review of materials used in tomographic volumetric additive manufacturing

Author(s): Jorge Madrid-Wolff, Joseph Toombs, Riccardo Rizzo, Paulina Nuñez Bernal, Dominique Porcincula, Rebecca Walton, Bin Wang, Frederik Kotz-Helmer, Yi Yang, David Kaplan, Yu Shrike Zhang, Marcy Zenobi-Wong, Robert R. McLeod, Bastian Rapp, Johanna Schwartz, Maxim Shusteff, Hayden Talyor, Riccardo Levato, Christophe Moser
Published in: MRS Communications, Issue 13, 2023, Page(s) 764-785, ISSN 2159-6867
Publisher: Springer Science and Business Media LLC
DOI: 10.1557/s43579-023-00447-x

Smart-watch-programmed green-light-operated percutaneous control of therapeutic transgenes

Author(s): Maysam Mansouri, Marie-Didiée Hussherr, Tobias Strittmatter, Peter Buchmann, Shuai Xue, Gieri Camenisch, Martin Fussenegger
Published in: Nature Communications, Issue 12/1, 2021, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-23572-4

Enhancing cell‐based therapies with synthetic gene circuits responsive to molecular stimuli

Author(s): Silvia Galvan, Ana P. Teixeira, Martin Fussenegger
Published in: Biotechnology and Bioengineering, Issue 121, 2025, Page(s) 2987-3000, ISSN 0006-3592
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.28770

Bioprinting and synthetic biology approaches to engineer functional endocrine pancreatic constructs

Author(s): Davide Ribezzi, Pere Català, Cataldo Pignatelli, Antonio Citro, Riccardo Levato
Published in: Trends in Biotechnology, 2025, ISSN 0167-7799
Publisher: Elsevier BV
DOI: 10.1016/j.tibtech.2025.03.005

Delivery Systems in Neuronal Direct Cell Reprogramming

Author(s): Giulia Redi, Filomena Del Piano, Sara Cappellini, Martina Paladino, Anne den Breejen, Marcel H.A.M. Fens, Massimiliano Caiazzo
Published in: Cellular Reprogramming, 2025, ISSN 2152-4971
Publisher: Mary Ann Liebert Inc.
DOI: 10.1089/cell.2025.0008

Brain Organoids: Filling the Need for a Human Model of Neurological Disorder

Author(s): Philip Jalink, Massimiliano Caiazzo
Published in: Biology, Issue 10, 2025, Page(s) 740, ISSN 2079-7737
Publisher: MDPI
DOI: 10.3390/biology10080740

A review of materials used in tomographic volumetric additive manufacturing

Author(s): Jorge Madrid-Wolff; Joseph Toombs; Riccardo Rizzo; Paulina Nuñez Bernal; Dominique Porcincula; Rebecca Walton; Bin Wang; Frederik Kotz-Helmer; Yi Yang; David Kaplan; Yu Shrike Zhang; Marcy Zenobi-Wong; Robert R. McLeod; Bastian Rapp; Johanna Schwartz; Maxim Shusteff; Hayden Talyor; Riccardo Levato; Christophe Moser
Published in: MRS Communications, Issue 3, 2023, ISSN 2159-6867
Publisher: Springer Link
DOI: 10.1557/s43579-023-00447-x

MicroRNA Roles in Cell Reprogramming Mechanisms

Author(s): Pascale, Emilia; Caiazza, Carmen; Paladino, Martina; Parisi, Silvia; Passaro, Fabiana; Caiazzo, Massimiliano
Published in: Cells, Issue 3, 2022, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/cells11060940

Breaking the resolution limits of 3D bioprinting: future opportunities and present challenges

Author(s): Tommaso Zandrini, Sammy Florczak, Riccardo Levato, and Aleksandr Ovsianikov
Published in: Trends in Biotechnology, 2022, ISSN 0167-7799
Publisher: Elsevier BV
DOI: 10.1016/j.tibtech.2022.10.009

Induced pluripotent stem cell-derived and directly reprogrammed neurons to study neurodegenerative diseases: The impact of aging signatures

Author(s): Simona Aversano, Carmen Caiazza, Massimiliano Caiazzo
Published in: Frontiers in Aging Neuroscience, 2022, ISSN 1663-4365
Publisher: Frontiers Research Foundation
DOI: 10.3389/fnagi.2022.1069482

Levodopa-loaded nanoparticles for the treatment of Parkinson's disease

Author(s): Emile F. van Vliet, Maarten J. Knol, Raymond M. Schiffelers, Massimiliano Caiazzo, Marcel H.A.M. Fens
Published in: Journal of Controlled Release, Issue 360, 2023, Page(s) 212-224, ISSN 0168-3659
Publisher: Elsevier BV
DOI: 10.1016/j.jconrel.2023.06.026

Volumetric Bioprinting of Organoids and Optically Tuned Hydrogels to Build Liver-Like Metabolic Biofactor

Author(s): Paulina Nuñez Bernal,Manon Bouwmeester,Jorge Madrid-Wolff,Marc Falandt,Sammy Florczak,Nuria Ginés Rodriguez,Yang Li,Gabriel Größbacher,Roos-Anne Samsom,Monique van Wolferen,Luc J. W. van der Laan,Paul Delrot,Damien Loterie,Jos Malda,Christophe Moser,Bart Spee,Riccardo Levato
Published in: Advanced Materials, 2022, ISSN 0935-9648
Publisher: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202110054

Programming Delayed Dissolution Into Sacrificial Bioinks For Dynamic Temporal Control of Architecture within 3D-Bioprinted Constructs

Author(s): Bram G. Soliman, Alessia Longoni, Mian Wang, Wanlu Li, Paulina N. Bernal, Alessandro Cianciosi, Gabriella C.J. Lindberg, Jos Malda, Juergen Groll, Tomasz Jungst, Riccardo Levato, Jelena Rnjak-Kovacina, Tim B. F. Woodfield, Yu Shrike Zhang, Khoon S. Lim
Published in: Advanced Functional Materials, 2023, ISSN 1616-301X
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/adfm.202210521

Design of programmable post-translational switch control platform for on-demand protein secretion in mammalian cells

Author(s): Maysam Mansouri, Preetam Guha Ray, Nik Franko, Shuai Xue, Martin Fussenegger
Published in: Nucleic Acids Research, 2023, ISSN 1362-4962
Publisher: Oxford University Press
DOI: 10.1093/nar/gkac916

Controlling Light in Scattering Materials for Volumetric Additive Manufacturing

Author(s): Madrid-Wolff, Jorge; Boniface, Antoine; Loterie, Damien; Delrot, Paul; Moser, Christophe
Published in: Advanced Science, Issue 1, 2022, ISSN 2198-3844
Publisher: Wiley
DOI: 10.1002/advs.202105144

Therapeutic cell engineering: designing programmable synthetic genetic circuits in mammalian cells

Author(s): Maysam Mansouri, Martin Fussenegger
Published in: Protein &amp; Cell, Issue 13, 2023, Page(s) 476-489, ISSN 1674-8018
Publisher: Oxford University Press (OUP)
DOI: 10.1007/s13238-021-00876-1

Small‐Molecule Regulators for Gene Switches to Program Mammalian Cell Behaviour

Author(s): Maysam Mansouri, Martin Fussenegger
Published in: ChemBioChem, Issue 25, 2025, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202300717

Light from Afield: Fast, High-Resolution, and Layer-Free Deep Vat 3D Printing

Author(s): Parth Chansoria, Riccardo Rizzo, Dominic Rütsche, Hao Liu, Paul Delrot, Marcy Zenobi-Wong
Published in: Chemical Reviews, Issue 124, 2024, Page(s) 8787-8822, ISSN 0009-2665
Publisher: American Chemical Society
DOI: 10.1021/acs.chemrev.4c00134

Engineering receptors in the secretory pathway for orthogonal signalling control

Author(s): Mohamed Mahameed, Pengli Wang, Shuai Xue & Martin Fussenegger
Published in: Nature Communications, 2022, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-022-35161-0

Volumetric Printing Across Melt Electrowritten Scaffolds Fabricates Multi-Material Living Constructs with Tunable Architecture and Mechanics

Author(s): Gabriel Größbacher, Michael Bartolf-Kopp, Csaba Gergely, Paulina Núñez Bernal, Sammy Florczak, Mylène de Ruijter, Núria Ginés Rodriguez, Jürgen Groll, Jos Malda, Tomasz Jungst, Riccardo Levato
Published in: Advanced Materials, 2023, ISSN 0935-9648
Publisher: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202300756

Inverse Rendering for Tomographic Volumetric Additive Manufacturing

Author(s): Baptiste Nicolet, Felix Wechsler, Jorge Madrid-Wolff, Christophe Moser, Wenzel Jakob
Published in: ACM Transactions on Graphics, Issue 43, 2024, Page(s) 1-17, ISSN 0730-0301
Publisher: Association for Computing Machinary, Inc.
DOI: 10.1145/3687924

The road ahead in materials and technologies for volumetric 3D printing

Author(s): Paulina Nunez Bernal, Sammy Florczak, Sebastian Inacker, Xiao Kuang, Jorge Madrid-Wolff, Martin Regehly, Stefan Hecht, Yu Shrike Zhang, Christophe Moser, Riccardo Levato
Published in: Nature Reviews Materials, 2025, ISSN 2058-8437
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/s41578-025-00785-3

Biofabricating the vascular tree in engineered bone tissue

Author(s): Leanne de Silva, Paulina N. Bernal, AJW Rosenberg, Jos Malda, Riccardo Levato, Debby Gawlitta
Published in: Acta Biomaterialia, 2023, Page(s) 250-268, ISSN 1742-7061
Publisher: Elsevier BV
DOI: 10.1016/j.actbio.2022.08.051

Other (1)

Exploring RRI in Bioprinted Organoids: Reflections from the ENLIGHT project

Author(s): Simone, Angela; Pellizzone, Anna
Published in: Issue 1, 2025
Publisher: Zenodo
DOI: 10.5281/zenodo.15372410

Book chapters (1)

Posttranslational Remote Control Mediated by Physical Inducers for Rapid Protein Release in Engineered Mammalian Cells

Author(s): Maysam Mansouri, Martin Fussenegger
Published in: Methods in Molecular Biology, Mammalian Synthetic Systems, 2024, Page(s) 233-241, ISBN 978-1-0716-3718-0
Publisher: Springer US
DOI: 10.1007/978-1-0716-3718-0_15

Non-peer reviewed articles (1)

Multi‐material Volumetric Bioprinting and Plug‐and‐play Suspension Bath Biofabrication via Bioresin Molecular Weight Tuning and via Multiwavelength Alignment Optics

Author(s): Davide Ribezzi, Jan‐Philip Zegwaart, Thomas Van Gansbeke, Aitor Tejo‐Otero, Sammy Florczak, Joska Aerts, Paul Delrot, Andreas Hierholzer, Martin Fussenegger, Jos Malda, Jos Olijve, Riccardo Levato
Published in: Advanced Materials, Issue 37, 2025, ISSN 0935-9648
Publisher: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202409355