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Overcoming the frontiers of biomolecular studies on human history and adaptation using palaeoproteomics

Project description

Palaeoproteomics for hominid evolution

A few decades ago, ancient DNA sequencing revolutionised research on the evolution and migration of archaic humans. More recent advances in palaeoproteomics (mass spectrometry-based ancient protein sequencing) combined with bioinformatic solutions have enabled similar progress in knowledge from molecular-based evolutionary reconstructions for species that became extinct millions of years ago. The EU-funded BACKWARD project aims to tackle the long-debated phylogenetic reconstruction of African and Asian hominins by developing and deploying a new generation of palaeoproteomic workflows. It will use state-of-the-art mass spectrometry and bioinformatics to address the phylogenetic relationships between South African early hominins and the hominid palaeobiodiversity in Southeast Asia. The project will bring substantial advances in palaeoanthropology, palaeontology, palaeoecology and archaeology.

Objective

BACKWARD will address major unsettled debates about African and Asian extinct hominid phylogeny, by developing and deploying a new generation of palaeoproteomic workflows, relying on the most advanced mass spectrometry and bioinformatic solutions currently available. Ancient DNA (aDNA) sequencing revolutionised our knowledge on evolution, migration and admixture of archaic and anatomically modern humans. However, no hominid aDNA older than ~0.4 million years has been retrieved yet. Ancient proteins instead survive much longer than aDNA, enabling molecular-based phylogeny beyond the limits of aDNA degradation. Recently, mass spectrometry (MS)-based ancient protein sequencing, i.e. palaeoproteomics, convincingly demonstrated its transformative value, enabling molecular-based evolutionary reconstructions for species that went extinct millions of years ago. BACKWARD will use palaeoproteomics to address: (i) the phylogenetic relationships among South African early hominins, and (ii) the hominid palaeobiodiversity in Southeast Asia; two topics debated for generations, and further complicated by recent finds. This knowledge will also provide the evolutionary scaffolding needed to correctly identify and correlate the series of processes that defined human brain expansion and reorganization. BACKWARD will also screen large sets of morphologically non-informative isolated fossil fragments of bones and teeth, to identify the species and sex of the organism from which they originated. Some of these solutions will be commercially re-purposed to deliver superior performance in public and private analytic laboratories for diagnostics in forensic medicine, and in the food or pharmaceutical industry. As a key BACKWARD feature, the unique contribution provided by each participating institution will be integrated in a strong partnership to transform palaeoanthropology, palaeontology, palaeoecology and archaeology once again, as aDNA did over the last twenty years.

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Programme(s)

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Topic(s)

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Funding Scheme

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ERC-ADG - Advanced Grant

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Call for proposal

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(opens in new window) ERC-2020-ADG

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Host institution

KOBENHAVNS UNIVERSITET
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 2 499 276,00
Address
NORREGADE 10
1165 KOBENHAVN
Denmark

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Region
Danmark Hovedstaden Byen København
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 2 499 276,00

Beneficiaries (1)

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