environmentAL Toxicity chEmical mixtuRes through aN innovative platform based on aged cardiac tissue model

Project Information

ALTERNATIVE

Grant agreement ID: 101037090

DOI

10.3030/101037090

Project closed

EC signature date 2 September 2021

Start date 1 October 2021

End date 31 March 2025

Funded under

• SOCIETAL CHALLENGES - Climate action, Environment, Resource Efficiency and Raw Materials

Total cost

€ 5 499 757,25

EU contribution

€ 5 499 757,25

Coordinated by

POLITECNICO DI TORINO
Italy

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Documents, reports (5)

Draft Guidance for regulatory use of the ALTERNATIVE platform

The guidance will include a detailed characterization of the individual ALTERNATIVE platform methods and describe how the data generated with these methods should be used by regulators, either as such or as a building block of the developed IATA for cardiotoxicity (D2.4). This deliverable is linked to T7.2 and could contain policy relevant information as it relates to how regulators should use/interpret information obtained by the ALTERNATIVE platform.

Intermediate dissemination and communication report

Provides an impact assessment of the dissemination and communication activities performed in the first 18 months of the project according to the plan outlined in D8.1. It describes the major performed activities and provides an updated plan for dissemination and communication in the second half of the project. This deliverable is linked to T8.1.

Documentation of regulatory needs for cardiotoxicity methods and data which can be used for consultation of national and European PARERE networks

Current regulatory data requirements across regulations will be reviewed and contextualized with ongoing European policy initiatives, scientific concern for cardiotoxicity and options for NAM based improvements. The resulting report shall be easily readable for regulators, shall contain questions for regulators and will be disseminated within the WP leads PARERE network of regulators. The resulting feedback will be evaluated. This deliverable is linked to T2.1 and is a policy related document.

Initial dissemination and communication plan

Describes the dissemination and communication strategy for the whole project and especially the first period up to M18. It describes the goals and target audiences and outlines the major planned dissemination and communication activities for the first half of the project. This deliverable is linked to T8.1.

Toxicological evidence

Systematic reviews for the identified compounds published as monographs and/or in scientific journals. This deliverable is linked to T3.2.

Open Research Data Pilot (1)

Data management plan

Outlines the data management policy and methods applicable to ALTERNATIVE data. It details actions for the overall control of data and summarises the types of data and sources, as well as how to handle the data. This deliverable is linked to T8.2.

Publications

Peer reviewed articles (9)

A systematic review and meta-analysis of human population studies on the association between exposure to toxic environmental chemicals and left ventricular dysfunction (LVD)

Author(s): G. Donzelli, F. Sera, M.A. Morales, F. Vozzi, T. Roos, A. Schaffert, M. Paparella, S. Murugadoss, B. Mertens, R. Gehring, N. Linzalone
Published in: Environmental Research, Issue 249, 2024, Page(s) 118429, ISSN 0013-9351
Publisher: Academic Press
DOI: 10.1016/j.envres.2024.118429

Use of Polyesters in Fused Deposition Modeling for Biomedical Applications

Author(s): Gianluca Ciardelli, Monica Boffito, Arianna Grivet-Brancot
Published in: Macromolecular bioscience, 2022, Page(s) -
Publisher: Wiley Online Library
DOI: 10.1002/mabi.202200039

Artificial Intelligence and Machine Learning Methods to Evaluate Cardiotoxicity following the Adverse Outcome Pathway Frameworks

Author(s): Edoardo Luca Viganò, Davide Ballabio, Alessandra Roncaglioni
Published in: Toxics, Issue 12, 2024, Page(s) 87, ISSN 2305-6304
Publisher: MDPI
DOI: 10.3390/toxics12010087

P14-03 Addressing cardiotoxicity in environmental chemical regulation: an integrated approach to cardiotoxicity testing

Author(s): A. Schaffert, S. Murugadoss, B. Mertens, M. Paparella
Published in: Toxicology Letters, Issue 399, 2024, Page(s) S226-S227, ISSN 0378-4274
Publisher: Elsevier BV
DOI: 10.1016/j.toxlet.2024.07.556

Cardiac tissue engineering: Multiple approaches and potential applications

Author(s): Ilaria Gisone, Antonella Cecchettini, Elisa Ceccherini, Elisa Persiani, Maria Aurora Morales, Federico Vozzi
Published in: Frontiers in Bioengineering and Biotechnology, Issue 3, 2022, Page(s) 1, ISSN 2296-4185
Publisher: Frontiers
DOI: 10.3389/fbioe.2022.980393

CEC01-01 Introduction into the AOP framework: Integrating Theory and Practice in AOP Development

Author(s): A. Schaffert, S. Murugadoss
Published in: Toxicology Letters, Issue 399, 2024, Page(s) S4, ISSN 0378-4274
Publisher: Elsevier BV
DOI: 10.1016/j.toxlet.2024.07.014

Ligand-based prediction of hERG-mediated cardiotoxicity based on the integration of different machine learning techniques

Author(s): Pietro Delre, Giovanna J. Lavado, Giuseppe Lamanna, Michele Saviano, Alessandra Roncaglioni, Emilio Benfenati, Giuseppe Felice Nagiatordi, Domenico Gadaleta
Published in: Frontiers in Pharmacology, Issue 13, 2022, Page(s) 1-13, ISSN 1663-9812
Publisher: Frontiers Media S.A.
DOI: 10.3389/fphar.2022.951083

Protocol for a Systematic Review and Meta-Analysis of Observational Studies on the Association of Exposure to Toxic Environmental Pollutants and Left Ventricular Dysfunction

Author(s): Nunzia Linzalone, Maria Aurora Morales, Federico Vozzi, Gabriele Donzelli
Published in: International Journal of Environmental Research and Public Health, Issue 19, 2022, Page(s) 1-8, ISSN 1661-7827
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijerph19127482

Linking nanomaterial-induced mitochondrial dysfunction to existing adverse outcome pathways for chemicals

Author(s): Sivakumar Murugadoss, Birgit Mertens, Martin Paparella, Alexandra Schaffert
Published in: Alternatives to Animal Experimentation, Issue Vol. 41 No. 1 (2024), 2024, Page(s) 14, ISSN 1868-8551
Publisher: ALTEX
DOI: 10.14573/altex.2305011

Conference proceedings (23)

Designing 3D bioengineered in vitro cardiac tissue models as reliable tools for the evaluation of chemical cardiotoxicity

Author(s): Monica Boffito, Susanna Sartori, Valeria Chiono, Federico Vozzi, Arianna Grivet-Brancot, Rossella Laurano,Roberta Pappalardo, Gianluca Ciardelli
Published in: ALTEX proceedings, Issue Volume 10, page 64, No. 2, (2022), 2022
Publisher: ALTEX Edition
DOI: 10.5281/zenodo.8367045

A Comprehensive Approach to Cardiotoxicity Assessment using Adverse Outcome Pathways

Author(s): A. Schaffert, S. Murugadoss, B. Mertens, T. Roos, R. Gehring, N. Linzalone, G. Donzelli, M. Paparella
Published in: ALTEX Proceedings, 2024
Publisher: ALTEX Edition
DOI: 10.58847/ap2402

Engineering 3D in vitro cardiac tissue models for cardiotoxicity assessment

Author(s): Vozzi, F., Boffito, M., Gisone, I., Cecchettini, A., Del Ry, S., Cabiati, M., Sartori, S., Laurano, R., Grivet-Brancot, A., Pappalardo, R., & Ciardelli, G.
Published in: 2022
Publisher: Biofabrication 2022
DOI: 10.5281/zenodo.8177565

Custom-made poly(ester urethane) tailored for 3D printing by Fused Deposition Modelling

Author(s): Gianluca Ciardelli, Andrea Alliaud, Ariana Grivet Brancot, Rosella Laurano, Susanna Sartori, Moncia Boffito
Published in: TERMIS, 2023, Page(s) 843
Publisher: TERMIS 2023 conference
DOI: 10.5281/zenodo.8186421

Innovative non-animal based testing platform to detect chemical-induced cardiotoxicity - A roadmap for regulatory uptake

Author(s): Sivakumar Murugadoss, Alexandra Schaffert, Ronette Gehring, Tom Roos, Nunzia Linzalone, Gabriele Donzelli, Martin Paparella, Birgit Mertens
Published in: 2023
Publisher: ASPIS Open Symposium 2023
DOI: 10.5281/zenodo.11382201

Tissue replica generation: the multifaceted contributions of cells and biomaterials

Author(s): Federico Vozzi
Published in: 2023
Publisher: ESB2023
DOI: 10.5281/zenodo.11367853

BIOENGINEERED 3D CARDIAC TISSUE MODEL FOR CARDIOTOXICITY STUDIES

Author(s): Ilaria Gisone, Andrea Alliaud, Elisa Persiani, Rossella Laurano, Elisa Ceccherini, Maria Aurora Morales, Monica Boffito, Antonella Cecchettini, Gianluca Ciardelli
Published in: Abstracts of the 2nd Microphysiological Systems World Summit 2023, 2023
Publisher: Altex
DOI: 10.58847/ap.2301

Design of 3D bioengineered cardiac tissue models for the evaluation of chemical cardiotoxicity

Author(s): Ciardelli, Gianluca; Grivet-Brancot, Arianna; Chiono, Valeria; Sartori, Susanne, & Boffito, Monica
Published in: 2022
Publisher: ESB 2022 conference
DOI: 10.5281/zenodo.8178217

ALTERNATIVE platform: an innovative tool for chemical cardiotoxicity detection

Author(s): Gianluca Ciardelli, Susanna Sartori, Andrea Alliaud, Arianna Grivet-Brancot, Monica Boffito, Silvia Del Ry, Manuela Cabiati, Elisa Persiani, Elisa Ceccherini, Antonella Cecchettini, Federico Vozzi
Published in: 2024
Publisher: Zenodo
DOI: 10.5281/zenodo.11353657

Multi-omics fingerprint of in vitro bioengineered heart-on-chip platform

Author(s): Ilaria Gisone, Maria Guirro, Lorena García, Nerea Abasolo, Federico Vozzi, Nuria Canela, Salvador Fernández Arroyo
Published in: 2024
Publisher: Zenodo
DOI: 10.5281/zenodo.11400752

3D printed PU/GelMA biphasic scaffold as cardiac tissue model.

Author(s): Susanna Sartori, Andrea Alliaud, Monica Boffito, Gianluca Ciardelli, Arianna Grivet-Brancot
Published in: 2024
Publisher: Zenodo
DOI: 10.5281/zenodo.11353780

The role of physiologically based kinetic modeling in interpreting the results of in vitro cell-based toxicity assays

Author(s): Ronette Gehring
Published in: ESB2022 Main abstract book, 2022, Page(s) 333
Publisher: ESB2022 conference
DOI: 10.5281/zenodo.8185809

Engineering of poly(urethane)-based porous constructs for cardiac tissue model design

Author(s): Andrea Alliaud, Monica Boffito, Rossella Laurano, Susanna Sartori, Gianluca Ciardelli
Published in: 2024
Publisher: Zenodo
DOI: 10.5281/zenodo.11350948

Proteomics reveals mechanisms of metabolic disruptive effects of emerging di-(2-ethylhexyl) phthalate substitutes

Author(s): Alexandra Schaffert, Isabel Karkossa, Elke Ueberham, Rita Schlichting, Katharina Walter, Josi Arnold, Matthias Blüher, John T. Heiker, Jörg Lehmann, Martin Wabitsch, Beate I. Escher, Martin von Bergen, Kristin Schubert
Published in: ALTEX proceedings, Issue Volume 10, No. 2, (2022), 2022, Page(s) 202, ISSN 2194-0479
Publisher: ALTEX
DOI: 10.5281/zenodo.8367087

Evidenze sull'associazione tra l'esposizione a sostanze tossiche ambientali e disfunzione ventricolare sinistra: protocollo per una revisione sistematica e meta-analisi di letteratura.

Author(s): Nunzia Linzalone, Gabriele Donzelli, Maria Aurora Morales, Federico Vozzi
Published in: 2022
Publisher: XLVI Convegno dell’Associazione Italiana di Epidemiologia. Decisioni in contesti di incertezza: il ruolo dell'epidemiologia
DOI: 10.5281/zenodo.8177446

Collagen-derived biomaterials in tissue engineering and organ modelling

Author(s): Ciardelli, Gianluca; Boffito, Monica; Tonda-Turo, Chiara; & Chiono, Valeria
Published in: 2022
Publisher: ESIB CONFERENCE
DOI: 10.5281/zenodo.8177934

3D printed PU/GelMA biphasic scaffold for detecting the cardiotoxicity of chemicals

Author(s): Susanna Sartori, Gianluca Ciardelli, Andrea Alliaud, Monica Boffito, Federico Vozzi, Ilaria Gisone, Antonella Cecchettini, Manuela Cabiati, Letizia Guiducci, Silvia Del Ry
Published in: 2022
Publisher: Zenodo
DOI: 10.5281/zenodo.11352529

LIFTING THE BURDEN FROM ANIMALS AND THE HEART: A COMPREHENSIVE APPROACH TO CARDIOTOXICITY EVALUATION

Author(s): Alexandra Schaffert, Sivakumar Murugadoss, Birgit Mertens, Tom Roos, Ronette Gehring, Nunzia Linzalone, Gabriele Donzelli, Martin Paparella
Published in: 2023
Publisher: Austrian 3R Center
DOI: 10.5281/zenodo.11350443

Co-culture of hiPSC-CMs and ECs to mimic cardiac tissue

Author(s): Ilaria Gisone, Manuela Cabiati, Elisa Ceccherini, Elisa Persiani, Letizia Guiducci, Antonella Cecchettini, Monica Boffito, Rossella Laurano, Gianluca Ciardelli, Federico Vozzi
Published in: 2023
Publisher: SIOoC
DOI: 10.5281/zenodo.11368020

From Data Exploration to Predictive Models: Advanced ML and AI Techniques for Cardiotoxicity Analysis

Author(s): Edoardo Viganò, Alessandra Roncaglioni
Published in: biomedical science and engineering, Issue Volume 4, No. 1, 2023, ISSN 2531-9892
Publisher: Pagepress
DOI: 10.4081/bse.226

ALTERNATIVE - Horizon 2020 project for building an innovative platform to assess the cardiotoxicity of chemicals

Author(s): Gianluca Ciardelli
Published in: 2022
Publisher: ESB 2022 conference
DOI: 10.5281/zenodo.11279640

Microfluidics for the pharmacological characterization of compounds on neurotransmitters transporters

Author(s): Francesca Romana Brugnoli, Benjamin Garlan
Published in: 2024
Publisher: Zenodo
DOI: 10.5281/zenodo.11350781

Reducing the Burden on Animals and the Heart: An Evidence-Based Approach to Cardiotoxicity

Author(s): Alexandra Schaffert, Sivakumar Murugadoss, Martin Paparella, Birgit Mertens, Tom Roos, Ronette Gehring, Nunzia Linzalone, Gabriele Donzelli
Published in: Abstracts of the 12th World Congress on Alternatives and Animal Use in the Life Sciences, Niagara Falls 2023, 2023
Publisher: ALTEX
DOI: 10.58847/ap.2302

Other (11)

White paper on REGULATION OF CHEMICAL EXPOSURES AND CARDIAC HEALTH: CURRENT GAPS AND FUTURE SOLUTIONS

Author(s): A. Schaffert, S. Murugadoss, M. Paprella, B. Mertens, A. Gavras, P. Mohnani
Published in: 2023
Publisher: Zenodo
DOI: 10.5281/zenodo.10021900

TAILOR-MADE POLYMERS: AN ADDITIONAL DEGREE OF FREEDOM IN THE TUNING OF MECHANICAL PROPERTIES IN TISSUE MODELING

Author(s): Gianluca Ciardelli
Published in: 2022
Publisher: Zenodo
DOI: 10.5281/zenodo.12705562

The role of physiologically-based kinetic (PBK) models within the new approach methodologies framework for predicting cardiotoxicity

Author(s): Ronette Gehring, Tom Roos, Zhicheng Zhang
Published in: 2022
Publisher: EUSAAT congress
DOI: 10.5281/zenodo.11281251

ALTERNATIVE project flyer

Author(s): Pooja Mohnani
Published in: 2021
Publisher: ALTERNATIVE

White paper on MPS

Author(s): Susanna Sartori, Federico Vozzi, Gianluca Ciardelli, Monica Boffito, Tommaso Sbrana, Miguel Berenguel Alonso, Salvador Fernández Arroyo, Adam Anglart
Published in: 2022
Publisher: ALTERNATIVE project
DOI: 10.5281/zenodo.7350571

Innovative non-animal based testing platform to detect chemical-induced cardiotoxicity - A roadmap for regulatory uptake

Author(s): Sivakumar Murugadoss, Birgit Mertens, Martin Paparella, Ronette Gehring, Nunzia Linzalone, Tom Roos, Alexandra Schaffert, Gabriele Donzelli
Published in: 2023
Publisher: EUROTOX 2023
DOI: 10.5281/zenodo.11381430

Achieving zero pollution by 2050 needs regulatory change: a call for policy support of New Approach Methodologies (NAMs)

Author(s): Martin Paparella, Alexandra Schaffert
Published in: 2024
Publisher: Zenodo
DOI: 10.5281/zenodo.13378369

Monitoring environmental parameters in microfluidic cell culture

Author(s): C. Betterelli Giuliano, L. Muiznieks, M. Kopf
Published in: 2022
Publisher: Zenodo
DOI: 10.5281/zenodo.14923212

Cardiotoxicity of chemicals: Current regulatory guidelines, knowledge gaps, and needs

Author(s): Alexandra Schaffert, Sivakumar Murugadoss, Birgit Mertens, Martin Paparella
Published in: 2023, Page(s) 337-340
Publisher: ALTEX
DOI: 10.14573/altex.2301121

WHITE PAPER ON ARTIFICIAL INTELLIGENCE (AI) AND MACHINE LEARNING (ML) APPLIED TO TOXICOLOGY FOR HUMAN HEALTH PROTECTION WITH A FOCUS ON EPIDEMIOLOGY

Author(s): N. Linzalone, F. Vozzi, B. Mertens
Published in: 2025
Publisher: Zenodo
DOI: 10.5281/zenodo.14832230

Stimulating the regulatory acceptance of a novel platform developed for the assessment of chemical-induced cardiotoxicity

Author(s): Sivakumar Murugadoss, Birgit Mertens, Martin Paparella, Ronette Gehring, Alexandra Schaffert, Tom Roos, Nunzia Linzalone
Published in: BELTOX ANNUAL MEETING 2022 ON THE CURRENT STATE OF KNOWLEDGE OF THE TOXICOLOGY AND ECOTOXICOLOGY OF PFAS, 2024
Publisher: Beltox
DOI: 10.5281/zenodo.11381282

Non-peer reviewed articles (1)

S11-03 New Approach Methodologies for cardiac toxicity assessment: the ALTERNATIVE perspective

Author(s): F. Vozzi
Published in: Toxicology Letters, Issue 399, 2024, Page(s) S25, ISSN 0378-4274
Publisher: Elsevier BV
DOI: 10.1016/j.toxlet.2024.07.079

Book chapters (1)

Artificial Intelligence Methods for Evaluating Mitochondrial Dysfunction: Exploring Various Chemical Notations Suitable for Neural Language Processing Models

Author(s): Edoardo Luca Viganò, Erika Colombo, Davide Ballabio, Alessandra Roncaglioni
Published in: Lecture Notes in Computer Science, AI in Drug Discovery, 2024, Page(s) 116-131
Publisher: Springer Nature Switzerland
DOI: 10.1007/978-3-031-72381-0_10