Molecular machines based on coiled-coil protein origami

Project Information

MaCChines

Grant agreement ID: 787115

DOI

10.3030/787115

Project closed

EC signature date 31 August 2018

Start date 1 September 2018

End date 31 August 2024

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 2 497 125,00

EU contribution

€ 2 497 125,00

Coordinated by

KEMIJSKI INSTITUT
Slovenia

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (19)

The art of designed coiled-coils for the regulation of mammalian cells

Author(s): Tjaša Plaper, Erik Rihtar, Taja Železnik Ramuta, Vida Forstnerič, Vid Jazbec, Filip Ivanovski, Mojca Benčina, Roman Jerala
Published in: Cell Chemical Biology, Issue 31, 2024, Page(s) 1460-1472, ISSN 2451-9456
Publisher: Springer Nature
DOI: 10.1016/j.chembiol.2024.06.001

Coiled Coils as Versatile Modules for Mammalian Cell Regulation

Author(s): Estera Merljak, Anja Golob-Urbanc, Tjaša Plaper, Roman Jerala
Published in: Synthetic Biology and Engineering, Issue 1, 2024, Page(s) 1-10, ISSN 2958-9053
Publisher: SCIEPublish
DOI: 10.35534/sbe.2023.10006

Subunits of an E3 Ligase Complex as Degrons for Efficient Degradation of Cytosolic, Nuclear, and Membrane Proteins

Author(s): Anže Verbič, Tina Lebar, Arne Praznik, Roman Jerala
Published in: ACS Synthetic Biology, Issue 13, 2024, Page(s) 792-803, ISSN 2161-5063
Publisher: American Chemical Society
DOI: 10.1021/acssynbio.3c00588

Designed allosteric protein logic

Author(s): Tjaša Plaper, Estera Merljak, Tina Fink, Tadej Satler, Ajasja Ljubetič, Duško Lainšček, Vid Jazbec, Mojca Benčina, Sintija Stevanoska, Sašo Džeroski, Roman Jerala
Published in: Cell Discovery, Issue 10, 2024, ISSN 2056-5968
Publisher: Springer Nature
DOI: 10.1038/s41421-023-00635-y

A nanobody toolbox targeting dimeric coiled-coilmodules for functionalization of designedprotein origami structures

Author(s): Andreja Majerle, San Hadži, Jana Aupič, Tadej Satler, Fabio Lapenta, Žiga Strmšek, Jurij Lah, Remy Loris, and Roman Jerala,
Published in: Proc Natl Acad Sci USA, 2021, ISSN 1091-6490
Publisher: National Academy of Science
DOI: 10.1073/pnas.2021899118

Coiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editing

Author(s): Duško Lainšček, Vida Forstnerič, Veronika Mikolič, Špela Malenšek, Peter Pečan, Mojca Benčina, Matjaž Sever, Helena Podgornik & Roman Jerala
Published in: Nature Communications, 2022, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-022-31386-1

Engineering a scalable and orthogonal platform for synthetic communication in mammalian cells

Author(s): Anna-Maria Makri Pistikou, Glenn A. O. Cremers, Bryan L. Nathalia, Theodorus J. Meuleman, Bas W. A. Bögels, Bruno V. Eijkens, Anne de Dreu, Maarten T. H. Bezembinder, Oscar M. J. A. Stassen, Carlijn C. V. Bouten, Maarten Merkx, Roman Jerala, Tom F. A. de Greef
Published in: Nature Communications, Issue 14, 2023, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-023-42810-5

Programmable de novo designed coiled coil-mediated phase separation in mammalian cells

Author(s): Maruša Ramšak, Dominique A. Ramirez, Loren E. Hough, Michael R. Shirts, Sara Vidmar, Kristina Eleršič Filipič, Gregor Anderluh, Roman Jerala
Published in: Nature Communications, Issue 14, 2023, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-023-43742-w

A multiplexed bacterial two-hybrid for rapid characterization of protein–protein interactions and iterative protein design

Author(s): W. Clifford Boldridge, Ajasja Ljubetič, Hwangbeom Kim, Nathan Lubock, Dániel Szilágyi, Jonathan Lee, Andrej Brodnik, Roman Jerala, Sriram Kosuri
Published in: Nature Communications, Issue 14, 2023, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-023-38697-x

A tunable orthogonal coiled-coil interaction toolbox for engineering mammalian cells

Author(s): Tina Lebar, Duško Lainšček, Estera Merljak, Jana Aupič, Roman Jerala
Published in: Nature Chemical Biology, 2020, ISSN 1552-4450
Publisher: Nature Publishing Group
DOI: 10.1038/s41589-019-0443-y

Design of fast proteolysis-based signaling and logic circuits in mammalian cells

Author(s): Tina Fink, Jan Lonzarić, Arne Praznik, Tjaša Plaper, Estera Merljak, Katja Leben, Nina Jerala, Tina Lebar, Žiga Strmšek, Fabio Lapenta, Mojca Benčina, Roman Jerala
Published in: Nature Chemical Biology, Issue 15/2, 2019, Page(s) 115-122, ISSN 1552-4450
Publisher: Nature Publishing Group
DOI: 10.1038/s41589-018-0181-6

Crystal Structure of <i>de Novo</i> Designed Coiled-Coil Protein Origami Triangle

Author(s): Tadej Satler, San Hadži, Roman Jerala
Published in: Journal of the American Chemical Society, Issue 145, 2023, Page(s) 16995-17000, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.3c05531

Regulation of protein secretion through chemicalregulation of endoplasmic reticulum retentionsignal cleavage

Author(s): Arne Praznik, Tina Fink, Nik Franko, Jan Lonzarić, Mojca Benčina, Nina Jerala, Tjaša Plaper, Samo Roškar & Roman Jerala
Published in: Nature Communications, 2022, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-022-28971-9

A Nanoscaffolded Spike-RBD Vaccine Provides Protectionagainst SARS-CoV-2 with Minimal Anti-Scaffold Response

Author(s): Duško Lainšček, Tina Fink, Vida Forstnerič, Iva Hafner-Bratkovič, Sara Orehek, Žiga Strmšek, Mateja Manček-Keber, Peter Pečan, Hana Esih, Špela Malenšek, Jana Aupič, Petra Dekleva, Tjaša Plaper, Sara Vidmar, Lucija Kadunc, Mojca Benčina, Neža Omersa, Gregor Anderluh, Florence Pojer, Kelvin Lau, David Hacker, Bruno E. Correia, David Peterhoff, Ralf Wagner, Valter Bergant, Alexander H
Published in: Vaccines, 2021, ISSN 2076-393X
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/vaccines9050431

Metal ion–regulated assembly of designedmodular protein cages

Author(s): Jana Aupič, Fabio Lapenta, Žiga Strmšek, Estera Merljak, Tjaša Plaper, Roman Jerala
Published in: Science Advances, 2022, ISSN 2375-2548
Publisher: AAAS
DOI: 10.1126/sciadv.abm8243

Preorganized cyclic modules facilitate the self-assembly of protein nanostructures

Author(s): Jaka Snoj, Fabio Lapenta, Roman Jerala
Published in: Chemical Science, Issue 15, 2024, Page(s) 3673-3686, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/d3sc06658d

Designed folding pathway of modular coiled-coil-based proteins

Author(s): Jana Aupič, Žiga Strmšek, Fabio Lapenta, David Pahovnik, Tomaž Pisanski, Igor Drobnak, Ajasja Ljubetič, Roman Jerala
Published in: Nature Communications, Issue 12/1, 2021, Page(s) 1-12, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-21185-5

Self-assembly and regulation of protein cages from pre-organised coiled-coil modules

Author(s): Fabio Lapenta, Jana Aupič, Marco Vezzoli, Žiga Strmšek, Stefano Da Vela, Dmitri I. Svergun, José María Carazo, Roberto Melero, Roman Jerala
Published in: Nature Communications, Issue 12/1, 2021, Page(s) 1-12, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-21184-6

Segmentation strategy of de novo designed four-helical bundles expands protein oligomerization modalities for cell regulation

Author(s): Estera Merljak, Benjamin Malovrh, Roman Jerala
Published in: Nature Communications, Issue 14, 2023, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-023-37765-6

Intellectual Property Rights

Patent (3)

REGULATION OF PROTEIN FUNCTION BY INSERTION OF INTERACTION PEPTIDES

Application/Publication number: 20 22085613
Date: 2022-12-13
Applicant(s): KEMIJSKI INSTITUT

VACCINES BASED ON AN ANTIGEN PROTEIN FUSED TO A NANOSTRUCTURING SCAFFOLD

Application/Publication number: 20 21073632
Date: 2021-08-26
Applicant(s): KEMIJSKI INSTITUT

COILED-COIL MEDIATED TETHERING OF CRISPR-CAS AND EXONUCLEASES FOR ENHANCED GENOME EDITING

Application/Publication number: 19 192490
Date: 2019-08-20
Applicant(s): KEMIJSKI INSTITUT