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GUT VIrus BRain Axis Technology In OrgaNoid Science

Project description

Complex organ-on-a-chip system for biomedical research

Organoid technology has achieved a breakthrough in biomedicine, with high potential for viral pathogenesis and antiviral research. The EU-funded GUTVIBRATIONS project will combine expertise in microfabrication, organoid technology, virology, immunology, and bioengineering to create an organ-on-a-chip system as a modular human gut-brain axis multi-organ model. This system will be built by combining 3D-printed scaffold technology with human gut and brain organoids. It will include a gut epithelial layer, immune cells, a blood-brain barrier and a brain organoid, connected by vertical stacking. The extracellular matrix (ECM) scaffold called Biosilk, a recombinant spider silk protein, will result in an in vivo like microenvironmental niche. The project aims to assess the system's commercialisation by a market analysis and a business plan.

Objective

The battle against infectious diseases is hampered by lack of therapeutic innovations due to poor understanding of disease outcome in humans. Organoid technology is a major breakthrough for medical research that has traditionally relied heavily on animal models. In our current OrganoVIR consortium, we are at the forefront in establishing human organoids as superior models for viral pathogenesis and antiviral research. The next critical step is to integrate organoid models to create a complex multi-organ ex vivo model that better mimics the human physiology. This raises several challenges such as incorporation of various cell types, medium incompatibility, validation, high throughput, and robust ways to connect mature organoid models. At the same time, the model has to be accessible, user friendly, and affordable to all end users. Therefore, this proposed GUTVIBRATIONS consortium will leverage its expertise in microfabrication, organoid technology, virology, immunology, bioengineering, and materials science to deliver an enabling organ-on-chip system.

As a demonstrator, a modular human gut-brain axis multi-organ model will be achieved for studying viral disease and treatment. This model will be built by combining an open source 3D printed scaffold technology with human gut and brain organoid models. The deliverables will include a complex gut epithelial layer, immune cell layer, blood-brain layer and brain organoid; all individually developed and subsequently connected by vertical stacking. Our unique modular approach where individual components are separately developed and validated minimizes compatibility issues. The individual models and the multi-organ model will be used for studying three different clinically relevant viral infections with high global burden and will be validated against existing clinical data to bring the system to TRL5. Routes towards commercialization will also be assessed by performing a market analysis and developing a business plan.

Fields of science (EuroSciVoc)

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Keywords

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Programme(s)

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Topic(s)

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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

RIA - Research and Innovation action

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Call for proposal

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(opens in new window) H2020-NMBP-TR-IND-2018-2020

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Coordinator

STICHTING AMSTERDAM UMC
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 2 187 375,00
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

No data

Participants (6)

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