Objective
Studies of the dynamical process of biological samples benefits from real-time imaging microscopy which can provide wide-field high resolution with sufficient material contrast. Hence, fluorescence-based microscopy has become one of the essential tools of modern biology. However, fluorescence techniques as other optical tool suffer from a fundamental resolution limit due to the wave nature of light. While resolution is denoted by the ability to discern different objects, much effort has been devoted to improve the spatial resolution of far-field fluorescence microscopy and it has spurred the emergence of many innovative techniques. Stimulated Emission Depletion (STED) microscopy has shown the best lateral resolution among the far-field techniques. However, based on point scanning, it is too slow to catch fast molecular dynamics in an image time series. Photo-activated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM) are equally powerful techniques that strictly spoken do not improve the spatial resolution, but the localization precision to pinpoint single molecules. The major drawback is that image acquisition is time consuming and thus makes them even less compatible for fast real-time imaging. Henceforth, the lifesciences community is still missing a microscopy technique that provides both high speed and super-resolution. To hurdle these limitations, we propose a fluorescence method that is based on artificial mesoscopic structures that are fully biocompatible and allow for super-resolution imaging of molecular dynamics in live cell with video-rate acquisition speed. Using an artificial mesoscopic structure one can manipulate light and design new components not previously realized such as a “perfect lens”. It has the potential for a breakthrough in biotechnology and may close the gap between Electron Microscopy (EM) and Fluorescence Microscopy (FM) even further to link structure and function of a biomolecule respectively.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules
- natural sciences physical sciences optics microscopy super resolution microscopy
- natural sciences physical sciences optics microscopy electron microscopy
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2011-IIF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
97070 Wuerzburg
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.